Restriction map for
pF1KB7518
(Circular) (Six-base) MAPSORT of: pF1KB7518.seq Check: 6097 from: 1 to: 3851
pF1KB7518 3851 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 889 889
Size: 3851
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 3691 3691
Size: 3851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1676 2108 2855 3123 826
Size: 850 432 747 268 1554
Fragments arranged by size:
1554 850 747 432 268
AflIII A'CryG_T
Cuts at: 238 633 1203 2307 3284 238
Size: 395 570 1104 977 805
Fragments arranged by size:
1104 977 805 570 395
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 389 389
Size: 3851
AlwNI CAG_nnn'CTG
Cuts at: 757 1156 2723 757
Size: 399 1567 1885
Fragments arranged by size:
1885 1567 399
ApaLI G'TGCA_C
Cuts at: 2621 2621
Size: 3851
ApoI r'AATT_y
Cuts at: 143 667 679 855 3204 3460 143
Size: 524 12 176 2349 256 534
Fragments arranged by size:
2349 534 524 256 176 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 871 871
Size: 3851
BamHI G'GATC_C
Cuts at: 167 876 167
Size: 709 3142
BanI G'GyrC_C
Cuts at: 429 867 1281 1529 1564 429
Size: 438 414 248 35 2716
Fragments arranged by size:
2716 438 414 248 35
BanII G_rGCy'C
Cuts at: 335 865 1895 335
Size: 530 1030 2291
Fragments arranged by size:
2291 1030 530
BbeI G_GCGC'C
Cuts at: 1285 1533 1285
Size: 248 3603
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2510 3851 1749
Size: 761 1341 1749
Fragments arranged by size:
1749 1341 761
BclI T'GATC_A
Cuts at: 1371 1371
Size: 3851
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3851
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 963 963
Size: 3851
Bme1580I G_kGCm'C
Cuts at: 1476 1569 2625 1476
Size: 93 1056 2702
Fragments arranged by size:
2702 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1144 1479 3623 1063
Size: 81 335 2144 1291
Fragments arranged by size:
2144 1291 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 3851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 719 434
Size: 285 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 1027 1906 2398 2696 311
Size: 716 879 492 298 1466
Fragments arranged by size:
1466 879 716 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 772 3555 772
Size: 2783 1068
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 1282 1530 2232 3728 1282
Size: 248 702 1496 1405
Fragments arranged by size:
1496 1405 702 248
BsaWI w'CCGG_w
Cuts at: 1040 1561 2239 2513 2660 3071 1040
Size: 521 678 274 147 411 1820
Fragments arranged by size:
1820 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 784
Size: 3851
BseYI C'CCAG_C
Cuts at: 138 1276 2034 2611 3107 138
Size: 1138 758 577 496 882
Fragments arranged by size:
1138 882 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 3851
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2647 104
Size: 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 46
BsiHKAI G_wGCw'C
Cuts at: 865 1643 1833 2625 865
Size: 778 190 792 2091
Fragments arranged by size:
2091 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 335 865 1476 1569 1643 1833 1895 2625 335
Size: 530 611 93 74 190 62 730 1561
Fragments arranged by size:
1561 730 611 530 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 561 3027 561
Size: 2466 1385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 901 1115 1417 1798 901
Size: 214 302 381 2954
Fragments arranged by size:
2954 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2143 2197 3681 3846 50
Size: 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 407 1763 407
Size: 1356 2495
BsrFI r'CCGG_y
Cuts at: 1849 2030 2239 1849
Size: 181 209 3461
Fragments arranged by size:
3461 209 181
BsrGI T'GTAC_A
Cuts at: 792 792
Size: 3851
BssSI C'ACGA_G
Cuts at: 2122 2480 2122
Size: 358 3493
BstAPI GCAn_nnn'nTGC
Cuts at: 1228 1228
Size: 3851
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstYI r'GATC_y
Cuts at: 1 167 876 1343 1366 1701 1947 2959 3045
Size: 166 709 467 23 335 246 1012 86
Cuts at: 3045 3057 3403 1
Size: 12 346 449
Fragments arranged by size:
1012 709 467 449 346 335 246 166
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 767 767
Size: 3851
BtgI C'CryG_G
Cuts at: 107 107
Size: 3851
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1801 1982 2145 1801
Size: 181 163 3507
Fragments arranged by size:
3507 181 163
BtsI GCAGTG_nn'
Cuts at: 479 1094 479
Size: 615 3236
DraI TTT'AAA
Cuts at: 850 850
Size: 3851
DrdI GACnn_nn'nnGTC
Cuts at: 1557 2415 1557
Size: 858 2993
EaeI y'GGCC_r
Cuts at: 1436 1610 2001 2028 3373 1436
Size: 174 391 27 1345 1914
Fragments arranged by size:
1914 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 560 1874 2084 560
Size: 1314 210 2327
Fragments arranged by size:
2327 1314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2059 2369 2515 3661 2059
Size: 310 146 1146 2249
Fragments arranged by size:
2249 1146 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 719 826 1676 2108 2855 3123 434
Size: 285 107 850 432 747 268 1162
Fragments arranged by size:
1162 850 747 432 285 268 107
EcoNI CCTnn'n_nnAGG
Cuts at: 533 533
Size: 3851
EcoO109I rG'GnC_Cy
Cuts at: 817 990 817
Size: 173 3678
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
EcoRV GAT'ATC
Cuts at: 123 523 123
Size: 400 3451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 239 1870 239
Size: 1631 2220
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 1285 1533 2555 3534 1285
Size: 248 1022 979 1602
Fragments arranged by size:
1602 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 1693 1723 1725 1755 427
Size: 32 1234 30 2 30 2523
Fragments arranged by size:
2523 1234 32 30 30 2
HincII GTy'rAC
Cuts at: 156 890 156
Size: 734 3117
KasI G'GCGC_C
Cuts at: 1281 1529 1281
Size: 248 3603
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 279 2522 2706 279
Size: 2243 184 1424
Fragments arranged by size:
2243 1424 184
MscI TGG'CCA
Cuts at: 1612 1612
Size: 3851
MslI CAynn'nnrTG
Cuts at: 1840 1967 1840
Size: 127 3724
MspA1I CmG'CkG
Cuts at: 363 962 1276 1636 2649 2894 363
Size: 599 314 360 1013 245 1320
Fragments arranged by size:
1320 1013 599 360 314 245
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 1282 1530 1282
Size: 248 3603
NcoI C'CATG_G
Cuts at: 107 107
Size: 3851
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 242 325 904 1404 2311 242
Size: 83 579 500 907 1782
Fragments arranged by size:
1782 907 579 500 83
PciI A'CATG_T
Cuts at: 238 2307 238
Size: 2069 1782
PfoI T'CCnGG_A
Cuts at: 194 194
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PsiI TTA'TAA
Cuts at: 451 451
Size: 3851
PstI C_TGCA'G
Cuts at: 474 898 1583 474
Size: 424 685 2742
Fragments arranged by size:
2742 685 424
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 1276 1636 1276
Size: 360 3491
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 560 1874 2084 560
Size: 1314 210 2327
Fragments arranged by size:
2327 1314 210
SbfI CC_TGCA'GG
Cuts at: 898 898
Size: 3851
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SfcI C'TryA_G
Cuts at: 33 470 894 1579 2572 2763 33
Size: 437 424 685 993 191 1121
Fragments arranged by size:
1121 993 685 437 424 191
SfoI GGC'GCC
Cuts at: 1283 1531 1283
Size: 248 3603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 873 873
Size: 3851
SmlI C'TyrA_G
Cuts at: 326 1006 1921 2413 2675 326
Size: 680 915 492 262 1502
Fragments arranged by size:
1502 915 680 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 325 904 1404 325
Size: 579 500 2772
Fragments arranged by size:
2772 579 500
StyI C'CwwG_G
Cuts at: 107 985 1339 107
Size: 878 354 2619
Fragments arranged by size:
2619 878 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 690 1434 1567 2026 2235 690
Size: 744 133 459 209 2306
Fragments arranged by size:
2306 744 459 209 133
TatI w'GTAC_w
Cuts at: 308 792 3366 308
Size: 484 2574 793
Fragments arranged by size:
2574 793 484
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 3819
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 457 882 457
Size: 425 3426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1346 114
Size: 1232 2619
XmaI C'CCGG_G
Cuts at: 871 871
Size: 3851
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI