Restriction map for
pF1KB7519
(Circular) (Six-base) MAPSORT of: pF1KB7519.seq Check: 7235 from: 1 to: 3539
pF1KB7519 3539 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 577 577
Size: 3539
Acc65I G'GTAC_C
Cuts at: 555 555
Size: 3539
AclI AA'CG_TT
Cuts at: 3379 3379
Size: 3539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1364 1796 2543 2811 1364
Size: 432 747 268 2092
Fragments arranged by size:
2092 747 432 268
AflIII A'CryG_T
Cuts at: 891 1995 2972 891
Size: 1104 977 1458
Fragments arranged by size:
1458 1104 977
AgeI A'CCGG_T
Cuts at: 1927 1927
Size: 3539
AlwNI CAG_nnn'CTG
Cuts at: 427 844 2411 427
Size: 417 1567 1555
Fragments arranged by size:
1567 1555 417
ApaLI G'TGCA_C
Cuts at: 2309 2309
Size: 3539
ApoI r'AATT_y
Cuts at: 207 543 2892 3148 207
Size: 336 2349 256 598
Fragments arranged by size:
2349 598 336 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
AvaI C'yCGr_G
Cuts at: 559 559
Size: 3539
BamHI G'GATC_C
Cuts at: 564 564
Size: 3539
BanI G'GyrC_C
Cuts at: 555 969 1217 1252 555
Size: 414 248 35 2842
Fragments arranged by size:
2842 414 248 35
BanII G_rGCy'C
Cuts at: 278 553 1583 278
Size: 275 1030 2234
Fragments arranged by size:
2234 1030 275
BbeI G_GCGC'C
Cuts at: 973 1221 973
Size: 248 3291
BbsI GAAGACnn'nnnn_
Cuts at: 279 279
Size: 3539
BbvCI CC'TCA_GC
Cuts at: 431 431
Size: 3539
BciVI GTATCCnnnnn_n'
Cuts at: 1437 2198 3539 1437
Size: 761 1341 1437
Fragments arranged by size:
1437 1341 761
BclI T'GATC_A
Cuts at: 1059 1059
Size: 3539
BglI GCCn_nnn'nGGC
Cuts at: 112 406 112
Size: 294 3245
BglII A'GATC_T
Cuts at: 1054 3091 1054
Size: 2037 1502
BlpI GC'TnA_GC
Cuts at: 651 651
Size: 3539
Bme1580I G_kGCm'C
Cuts at: 1164 1257 2313 1164
Size: 93 1056 2390
Fragments arranged by size:
2390 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 751 832 1167 3311 751
Size: 81 335 2144 979
Fragments arranged by size:
2144 979 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 174 1906 174
Size: 1732 1807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 355
Size: 3539
Bpu10I CC'TnA_GC
Cuts at: 431 431
Size: 3539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1594 2086 2384 715
Size: 879 492 298 1870
Fragments arranged by size:
1870 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 334 3243 334
Size: 2909 630
BsaAI yAC'GTr
Cuts at: 892 892
Size: 3539
BsaBI GATnn'nnATC
Cuts at: 6 1053 3090 6
Size: 1047 2037 455
Fragments arranged by size:
2037 1047 455
BsaHI Gr'CG_yC
Cuts at: 155 970 1218 1920 3416 155
Size: 815 248 702 1496 278
Fragments arranged by size:
1496 815 702 278 248
BsaWI w'CCGG_w
Cuts at: 728 1249 1927 2201 2348 2759 728
Size: 521 678 274 147 411 1508
Fragments arranged by size:
1508 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 454 484 454
Size: 30 3509
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 448 496 163
Size: 285 48 3206
Fragments arranged by size:
3206 285 48
BseYI C'CCAG_C
Cuts at: 964 1722 2299 2795 964
Size: 758 577 496 1708
Fragments arranged by size:
1708 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 278
Size: 3539
BsiEI CG_ry'CG
Cuts at: 104 1081 1127 2335 104
Size: 977 46 1208 1308
Fragments arranged by size:
1308 1208 977 46
BsiHKAI G_wGCw'C
Cuts at: 278 553 1331 1521 2313 278
Size: 275 778 190 792 1504
Fragments arranged by size:
1504 792 778 275 190
Bsp1286I G_dGCh'C
Cuts at: 278 553 1164 1257 1331 1521 1583 2313 278
Size: 275 611 93 74 190 62 730 1504
Fragments arranged by size:
1504 730 611 275 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2759 2759
Size: 3539
BspHI T'CATG_A
Cuts at: 290 2715 290
Size: 2425 1114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 589 803 1105 1486 589
Size: 214 302 381 2642
Fragments arranged by size:
2642 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1831 1885 3369 3534 50
Size: 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 344 1451 344
Size: 1107 2432
BsrFI r'CCGG_y
Cuts at: 1537 1718 1927 1537
Size: 181 209 3149
Fragments arranged by size:
3149 209 181
BssSI C'ACGA_G
Cuts at: 1810 2168 1810
Size: 358 3181
BstAPI GCAn_nnn'nTGC
Cuts at: 916 916
Size: 3539
BstBI TT'CG_AA
Cuts at: 1900 1900
Size: 3539
BstYI r'GATC_y
Cuts at: 1 564 1031 1054 1389 1635 2647 2733 2745
Size: 563 467 23 335 246 1012 86 12
Cuts at: 2745 3091 1
Size: 346 449
Fragments arranged by size:
1012 563 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 130 107
Size: 23 3516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1489 1670 1833 1489
Size: 181 163 3195
Fragments arranged by size:
3195 181 163
BtsI GCAGTG_nn'
Cuts at: 782 782
Size: 3539
DraI TTT'AAA
Cuts at: 538 538
Size: 3539
DrdI GACnn_nn'nnGTC
Cuts at: 1245 2103 1245
Size: 858 2681
EaeI y'GGCC_r
Cuts at: 407 1124 1298 1689 1716 3061 407
Size: 717 174 391 27 1345 885
Fragments arranged by size:
1345 885 717 391 174 27
EagI C'GGCC_G
Cuts at: 1124 1124
Size: 3539
EarI CTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1747 2057 2203 3349 1747
Size: 310 146 1146 1937
Fragments arranged by size:
1937 1146 310 146
EcoICRI GAG'CTC
Cuts at: 276 551 276
Size: 275 3264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 1364 1796 2543 2811 355
Size: 1009 432 747 268 1083
Fragments arranged by size:
1083 1009 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 678 678
Size: 3539
EcoRI G'AATT_C
Cuts at: 543 543
Size: 3539
EcoRV GAT'ATC
Cuts at: 507 507
Size: 3539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 540 1558 540
Size: 1018 2521
FspI TGC'GCA
Cuts at: 1320 1320
Size: 3539
HaeII r_GCGC'y
Cuts at: 973 1221 2243 3222 973
Size: 248 1022 979 1290
Fragments arranged by size:
1290 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1381 1411 1413 1443 1381
Size: 30 2 30 3477
Fragments arranged by size:
3477 30 30 2
HincII GTy'rAC
Cuts at: 578 578
Size: 3539
HindIII A'AGCT_T
Cuts at: 379 456 379
Size: 77 3462
KasI G'GCGC_C
Cuts at: 969 1217 969
Size: 248 3291
KpnI G_GTAC'C
Cuts at: 559 559
Size: 3539
MluI A'CGCG_T
Cuts at: 2972 2972
Size: 3539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2394 2210
Size: 184 3355
MscI TGG'CCA
Cuts at: 409 1300 409
Size: 891 2648
MslI CAynn'nnrTG
Cuts at: 1528 1655 1528
Size: 127 3412
MspA1I CmG'CkG
Cuts at: 132 193 650 964 1324 2337 2582 132
Size: 61 457 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 457 360 314 245 61
NaeI GCC'GGC
Cuts at: 1720 1720
Size: 3539
NarI GG'CG_CC
Cuts at: 970 1218 970
Size: 248 3291
NcoI C'CATG_G
Cuts at: 107 107
Size: 3539
NdeI CA'TA_TG
Cuts at: 221 221
Size: 3539
NgoMIV G'CCGG_C
Cuts at: 1718 1718
Size: 3539
NspI r_CATG'y
Cuts at: 592 1092 1999 592
Size: 500 907 2132
Fragments arranged by size:
2132 907 500
PciI A'CATG_T
Cuts at: 1995 1995
Size: 3539
PmeI GTTT'AAAC
Cuts at: 538 538
Size: 3539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 260 292 2678 2710 260
Size: 32 2386 32 1089
Fragments arranged by size:
2386 1089 32 32
PstI C_TGCA'G
Cuts at: 491 586 1271 491
Size: 95 685 2759
Fragments arranged by size:
2759 685 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3539
PvuII CAG'CTG
Cuts at: 193 964 1324 193
Size: 771 360 2408
Fragments arranged by size:
2408 771 360
RsrII CG'GwC_CG
Cuts at: 1734 1734
Size: 3539
SacI G_AGCT'C
Cuts at: 278 553 278
Size: 275 3264
SacII CC_GC'GG
Cuts at: 133 133
Size: 3539
SalI G'TCGA_C
Cuts at: 576 576
Size: 3539
SapI GCTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
SbfI CC_TGCA'GG
Cuts at: 586 586
Size: 3539
ScaI AGT'ACT
Cuts at: 3056 3056
Size: 3539
SfcI C'TryA_G
Cuts at: 33 487 582 1267 2260 2451 33
Size: 454 95 685 993 191 1121
Fragments arranged by size:
1121 993 685 454 191 95
SfiI GGCCn_nnn'nGGCC
Cuts at: 406 406
Size: 3539
SfoI GGC'GCC
Cuts at: 971 1219 971
Size: 248 3291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
SmaI CCC'GGG
Cuts at: 561 561
Size: 3539
SmlI C'TyrA_G
Cuts at: 694 1609 2101 2363 694
Size: 915 492 262 1870
Fragments arranged by size:
1870 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3539
SphI G_CATG'C
Cuts at: 592 1092 592
Size: 500 3039
StyI C'CwwG_G
Cuts at: 107 440 673 1027 107
Size: 333 233 354 2619
Fragments arranged by size:
2619 354 333 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 1255 1714 1923 1122
Size: 133 459 209 2738
Fragments arranged by size:
2738 459 209 133
TatI w'GTAC_w
Cuts at: 3054 3054
Size: 3539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3507
Tth111I GACn'n_nGTC
Cuts at: 1336 1336
Size: 3539
XbaI T'CTAG_A
Cuts at: 570 570
Size: 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1034 1034
Size: 3539
XmaI C'CCGG_G
Cuts at: 559 559
Size: 3539
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI