Restriction map for
pF1KB7521
(Circular) (Six-base) MAPSORT of: pF1KB7521.seq Check: 6787 from: 1 to: 3545
pF1KB7521 3545 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 583 583
Size: 3545
Acc65I G'GTAC_C
Cuts at: 511 561 511
Size: 50 3495
AclI AA'CG_TT
Cuts at: 3385 3385
Size: 3545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 517 1370 1802 2549 2817 115
Size: 402 853 432 747 268 843
Fragments arranged by size:
853 843 747 432 402 268
AflIII A'CryG_T
Cuts at: 412 897 2001 2978 412
Size: 485 1104 977 979
Fragments arranged by size:
1104 979 977 485
AgeI A'CCGG_T
Cuts at: 1933 1933
Size: 3545
AlwNI CAG_nnn'CTG
Cuts at: 850 2417 850
Size: 1567 1978
ApaLI G'TGCA_C
Cuts at: 2315 2315
Size: 3545
ApoI r'AATT_y
Cuts at: 144 549 2898 3154 144
Size: 405 2349 256 535
Fragments arranged by size:
2349 535 405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3545
AvaI C'yCGr_G
Cuts at: 565 565
Size: 3545
BamHI G'GATC_C
Cuts at: 570 570
Size: 3545
BanI G'GyrC_C
Cuts at: 511 561 975 1223 1258 511
Size: 50 414 248 35 2798
Fragments arranged by size:
2798 414 248 50 35
BanII G_rGCy'C
Cuts at: 559 1589 559
Size: 1030 2515
BbeI G_GCGC'C
Cuts at: 979 1227 979
Size: 248 3297
BbsI GAAGACnn'nnnn_
Cuts at: 505 505
Size: 3545
BciVI GTATCCnnnnn_n'
Cuts at: 1443 2204 3545 1443
Size: 761 1341 1443
Fragments arranged by size:
1443 1341 761
BclI T'GATC_A
Cuts at: 1065 1065
Size: 3545
BglII A'GATC_T
Cuts at: 1060 3097 1060
Size: 2037 1508
BlpI GC'TnA_GC
Cuts at: 657 657
Size: 3545
Bme1580I G_kGCm'C
Cuts at: 1170 1263 2319 1170
Size: 93 1056 2396
Fragments arranged by size:
2396 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 332 757 838 1173 3317 332
Size: 425 81 335 2144 560
Fragments arranged by size:
2144 560 425 335 81
BmtI G_CTAG'C
Cuts at: 374 374
Size: 3545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1912 1912
Size: 3545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 472 439
Size: 33 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1600 2092 2390 721
Size: 879 492 298 1876
Fragments arranged by size:
1876 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3249 3249
Size: 3545
BsaAI yAC'GTr
Cuts at: 898 898
Size: 3545
BsaBI GATnn'nnATC
Cuts at: 6 1059 3096 6
Size: 1053 2037 455
Fragments arranged by size:
2037 1053 455
BsaHI Gr'CG_yC
Cuts at: 976 1224 1926 3422 976
Size: 248 702 1496 1099
Fragments arranged by size:
1496 1099 702 248
BsaWI w'CCGG_w
Cuts at: 734 1255 1933 2207 2354 2765 734
Size: 521 678 274 147 411 1514
Fragments arranged by size:
1514 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 452 452
Size: 3545
BseYI C'CCAG_C
Cuts at: 272 970 1728 2305 2801 272
Size: 698 758 577 496 1016
Fragments arranged by size:
1016 758 698 577 496
BsiEI CG_ry'CG
Cuts at: 104 1087 1133 2341 104
Size: 983 46 1208 1308
Fragments arranged by size:
1308 1208 983 46
BsiHKAI G_wGCw'C
Cuts at: 559 1337 1527 2319 559
Size: 778 190 792 1785
Fragments arranged by size:
1785 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 559 1170 1263 1337 1527 1589 2319 559
Size: 611 93 74 190 62 730 1785
Fragments arranged by size:
1785 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2765 2765
Size: 3545
BspHI T'CATG_A
Cuts at: 2721 2721
Size: 3545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 595 809 1111 1492 595
Size: 214 302 381 2648
Fragments arranged by size:
2648 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1837 1891 3375 3540 50
Size: 1787 54 1484 165 55
Fragments arranged by size:
1787 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 196 1457 196
Size: 1261 2284
BsrFI r'CCGG_y
Cuts at: 1543 1724 1933 1543
Size: 181 209 3155
Fragments arranged by size:
3155 209 181
BssSI C'ACGA_G
Cuts at: 1816 2174 1816
Size: 358 3187
BstAPI GCAn_nnn'nTGC
Cuts at: 292 922 292
Size: 630 2915
BstBI TT'CG_AA
Cuts at: 1906 1906
Size: 3545
BstYI r'GATC_y
Cuts at: 1 570 1037 1060 1395 1641 2653 2739 2751
Size: 569 467 23 335 246 1012 86 12
Cuts at: 2751 3097 1
Size: 346 449
Fragments arranged by size:
1012 569 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3545
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1495 1676 1839 1495
Size: 181 163 3201
Fragments arranged by size:
3201 181 163
BtsI GCAGTG_nn'
Cuts at: 788 788
Size: 3545
DraI TTT'AAA
Cuts at: 544 544
Size: 3545
DrdI GACnn_nn'nnGTC
Cuts at: 1251 2109 1251
Size: 858 2687
EaeI y'GGCC_r
Cuts at: 1130 1304 1695 1722 3067 1130
Size: 174 391 27 1345 1608
Fragments arranged by size:
1608 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1130 1130
Size: 3545
EarI CTCTTCn'nnn_
Cuts at: 257 533 1568 1778 257
Size: 276 1035 210 2024
Fragments arranged by size:
2024 1035 276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1753 2063 2209 3355 127
Size: 1626 310 146 1146 317
Fragments arranged by size:
1626 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 557 557
Size: 3545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 439 472 517 1370 1802 2549 2817 115
Size: 324 33 45 853 432 747 268 843
Fragments arranged by size:
853 843 747 432 324 268 45 33
EcoO109I rG'GnC_Cy
Cuts at: 684 684
Size: 3545
EcoRI G'AATT_C
Cuts at: 144 549 144
Size: 405 3140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1564 1564
Size: 3545
FspI TGC'GCA
Cuts at: 1326 1326
Size: 3545
HaeII r_GCGC'y
Cuts at: 979 1227 2249 3228 979
Size: 248 1022 979 1296
Fragments arranged by size:
1296 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1387 1417 1419 1449 1387
Size: 30 2 30 3483
Fragments arranged by size:
3483 30 30 2
HincII GTy'rAC
Cuts at: 584 584
Size: 3545
KasI G'GCGC_C
Cuts at: 975 1223 975
Size: 248 3297
KpnI G_GTAC'C
Cuts at: 515 565 515
Size: 50 3495
MluI A'CGCG_T
Cuts at: 2978 2978
Size: 3545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2400 2216
Size: 184 3361
MscI TGG'CCA
Cuts at: 1306 1306
Size: 3545
MslI CAynn'nnrTG
Cuts at: 1534 1661 1534
Size: 127 3418
MspA1I CmG'CkG
Cuts at: 355 495 656 970 1330 2343 2588 355
Size: 140 161 314 360 1013 245 1312
Fragments arranged by size:
1312 1013 360 314 245 161 140
NaeI GCC'GGC
Cuts at: 1726 1726
Size: 3545
NarI GG'CG_CC
Cuts at: 976 1224 976
Size: 248 3297
NcoI C'CATG_G
Cuts at: 107 107
Size: 3545
NgoMIV G'CCGG_C
Cuts at: 1724 1724
Size: 3545
NheI G'CTAG_C
Cuts at: 370 370
Size: 3545
NspI r_CATG'y
Cuts at: 416 447 598 1098 2005 416
Size: 31 151 500 907 1956
Fragments arranged by size:
1956 907 500 151 31
PciI A'CATG_T
Cuts at: 412 2001 412
Size: 1589 1956
PmeI GTTT'AAAC
Cuts at: 544 544
Size: 3545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2684 2716 2684
Size: 32 3513
PstI C_TGCA'G
Cuts at: 592 1277 592
Size: 685 2860
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3545
PvuII CAG'CTG
Cuts at: 355 495 970 1330 355
Size: 140 475 360 2570
Fragments arranged by size:
2570 475 360 140
RsrII CG'GwC_CG
Cuts at: 1740 1740
Size: 3545
SacI G_AGCT'C
Cuts at: 559 559
Size: 3545
SalI G'TCGA_C
Cuts at: 582 582
Size: 3545
SapI GCTCTTCn'nnn_
Cuts at: 1568 1778 1568
Size: 210 3335
SbfI CC_TGCA'GG
Cuts at: 592 592
Size: 3545
ScaI AGT'ACT
Cuts at: 3062 3062
Size: 3545
SfcI C'TryA_G
Cuts at: 33 257 350 588 1273 2266 2457 33
Size: 224 93 238 685 993 191 1121
Fragments arranged by size:
1121 993 685 238 224 191 93
SfoI GGC'GCC
Cuts at: 977 1225 977
Size: 248 3297
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3545
SmaI CCC'GGG
Cuts at: 567 567
Size: 3545
SmlI C'TyrA_G
Cuts at: 700 1615 2107 2369 700
Size: 915 492 262 1876
Fragments arranged by size:
1876 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3545
SphI G_CATG'C
Cuts at: 598 1098 598
Size: 500 3045
SspI AAT'ATT
Cuts at: 219 219
Size: 3545
StyI C'CwwG_G
Cuts at: 107 679 1033 107
Size: 572 354 2619
Fragments arranged by size:
2619 572 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1128 1261 1720 1929 1128
Size: 133 459 209 2744
Fragments arranged by size:
2744 459 209 133
TatI w'GTAC_w
Cuts at: 3060 3060
Size: 3545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3513
Tth111I GACn'n_nGTC
Cuts at: 1342 1342
Size: 3545
XbaI T'CTAG_A
Cuts at: 576 576
Size: 3545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1040 1040
Size: 3545
XmaI C'CCGG_G
Cuts at: 565 565
Size: 3545
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI