Restriction map for
pF1KB7522
(Circular) (Six-base) MAPSORT of: pF1KB7522.seq Check: 1517 from: 1 to: 3860
pF1KB7522 3860 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 898 898
Size: 3860
Acc65I G'GTAC_C
Cuts at: 876 876
Size: 3860
AclI AA'CG_TT
Cuts at: 3700 3700
Size: 3860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 766 832 1685 2117 2864 3132 311
Size: 455 66 853 432 747 268 1039
Fragments arranged by size:
1039 853 747 455 432 268 66
AflII C'TTAA_G
Cuts at: 703 703
Size: 3860
AflIII A'CryG_T
Cuts at: 651 1212 2316 3293 651
Size: 561 1104 977 1218
Fragments arranged by size:
1218 1104 977 561
AgeI A'CCGG_T
Cuts at: 2248 2248
Size: 3860
AleI CACnn'nnGTG
Cuts at: 157 157
Size: 3860
AlwNI CAG_nnn'CTG
Cuts at: 1165 2732 1165
Size: 1567 2293
ApaLI G'TGCA_C
Cuts at: 2630 2630
Size: 3860
ApoI r'AATT_y
Cuts at: 864 3213 3469 864
Size: 2349 256 1255
Fragments arranged by size:
2349 1255 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
AvaI C'yCGr_G
Cuts at: 635 880 635
Size: 245 3615
BamHI G'GATC_C
Cuts at: 788 885 788
Size: 97 3763
BanI G'GyrC_C
Cuts at: 139 876 1290 1538 1573 139
Size: 737 414 248 35 2426
Fragments arranged by size:
2426 737 414 248 35
BanII G_rGCy'C
Cuts at: 406 636 874 1904 406
Size: 230 238 1030 2362
Fragments arranged by size:
2362 1030 238 230
BbeI G_GCGC'C
Cuts at: 143 1294 1542 143
Size: 1151 248 2461
Fragments arranged by size:
2461 1151 248
BbsI GAAGACnn'nnnn_
Cuts at: 503 626 503
Size: 123 3737
BciVI GTATCCnnnnn_n'
Cuts at: 1758 2519 3860 1758
Size: 761 1341 1758
Fragments arranged by size:
1758 1341 761
BclI T'GATC_A
Cuts at: 1380 1380
Size: 3860
BglII A'GATC_T
Cuts at: 1375 3412 1375
Size: 2037 1823
BlpI GC'TnA_GC
Cuts at: 972 972
Size: 3860
Bme1580I G_kGCm'C
Cuts at: 1485 1578 2634 1485
Size: 93 1056 2711
Fragments arranged by size:
2711 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1072 1153 1488 3632 1072
Size: 81 335 2144 1300
Fragments arranged by size:
2144 1300 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 3860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 1036 1915 2407 2705 236
Size: 800 879 492 298 1391
Fragments arranged by size:
1391 879 800 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3564 3564
Size: 3860
BsaAI yAC'GTr
Cuts at: 1213 1213
Size: 3860
BsaBI GATnn'nnATC
Cuts at: 6 1374 3411 6
Size: 1368 2037 455
Fragments arranged by size:
2037 1368 455
BsaHI Gr'CG_yC
Cuts at: 140 1291 1539 2241 3737 140
Size: 1151 248 702 1496 263
Fragments arranged by size:
1496 1151 702 263 248
BsaWI w'CCGG_w
Cuts at: 1049 1570 2248 2522 2669 3080 1049
Size: 521 678 274 147 411 1829
Fragments arranged by size:
1829 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1285 2043 2620 3116 1285
Size: 758 577 496 2029
Fragments arranged by size:
2029 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1402 1448 2656 104
Size: 1298 46 1208 1308
Fragments arranged by size:
1308 1298 1208 46
BsiHKAI G_wGCw'C
Cuts at: 339 406 636 874 1652 1842 2634 339
Size: 67 230 238 778 190 792 1565
Fragments arranged by size:
1565 792 778 238 230 190 67
BsmI GAATG_Cn'
Cuts at: 457 457
Size: 3860
Bsp1286I G_dGCh'C
Cuts at: 339 406 636 874 1485 1578 1652 1842 1904
Size: 67 230 238 611 93 74 190 62
Cuts at: 1904 2634 339
Size: 730 1565
Fragments arranged by size:
1565 730 611 238 230 190 93 74
67 62
BspEI T'CCGG_A
Cuts at: 3080 3080
Size: 3860
BspHI T'CATG_A
Cuts at: 303 3036 303
Size: 2733 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 910 1124 1426 1807 910
Size: 214 302 381 2963
Fragments arranged by size:
2963 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2152 2206 3690 3855 50
Size: 2102 54 1484 165 55
Fragments arranged by size:
2102 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 202 1772 202
Size: 1570 2290
BsrFI r'CCGG_y
Cuts at: 136 1858 2039 2248 136
Size: 1722 181 209 1748
Fragments arranged by size:
1748 1722 209 181
BssSI C'ACGA_G
Cuts at: 2131 2489 2131
Size: 358 3502
BstAPI GCAn_nnn'nTGC
Cuts at: 1237 1237
Size: 3860
BstBI TT'CG_AA
Cuts at: 2221 2221
Size: 3860
BstYI r'GATC_y
Cuts at: 1 288 788 885 1352 1375 1710 1956 2968
Size: 287 500 97 467 23 335 246 1012
Cuts at: 2968 3054 3066 3412 1
Size: 86 12 346 449
Fragments arranged by size:
1012 500 467 449 346 335 287 246
97 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1810 1991 2154 1810
Size: 181 163 3516
Fragments arranged by size:
3516 181 163
BtsI GCAGTG_nn'
Cuts at: 1103 1103
Size: 3860
DraI TTT'AAA
Cuts at: 859 859
Size: 3860
DrdI GACnn_nn'nnGTC
Cuts at: 267 1566 2424 267
Size: 1299 858 1703
Fragments arranged by size:
1703 1299 858
EaeI y'GGCC_r
Cuts at: 110 1445 1619 2010 2037 3382 110
Size: 1335 174 391 27 1345 588
Fragments arranged by size:
1345 1335 588 391 174 27
EagI C'GGCC_G
Cuts at: 1445 1445
Size: 3860
EarI CTCTTCn'nnn_
Cuts at: 210 500 1883 2093 210
Size: 290 1383 210 1977
Fragments arranged by size:
1977 1383 290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2068 2378 2524 3670 2068
Size: 310 146 1146 2258
Fragments arranged by size:
2258 1146 310 146
EcoICRI GAG'CTC
Cuts at: 404 634 872 404
Size: 230 238 3392
Fragments arranged by size:
3392 238 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 766 832 1685 2117 2864 3132 311
Size: 455 66 853 432 747 268 1039
Fragments arranged by size:
1039 853 747 455 432 268 66
EcoO109I rG'GnC_Cy
Cuts at: 999 999
Size: 3860
EcoRI G'AATT_C
Cuts at: 864 864
Size: 3860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 771 1879 771
Size: 1108 2752
FspI TGC'GCA
Cuts at: 1641 1641
Size: 3860
HaeII r_GCGC'y
Cuts at: 143 1294 1542 2564 3543 143
Size: 1151 248 1022 979 460
Fragments arranged by size:
1151 1022 979 460 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 403 423 435 1702 1732 1734 1764 391
Size: 12 20 12 1267 30 2 30 2487
Fragments arranged by size:
2487 1267 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 899 899
Size: 3860
KasI G'GCGC_C
Cuts at: 139 1290 1538 139
Size: 1151 248 2461
Fragments arranged by size:
2461 1151 248
KpnI G_GTAC'C
Cuts at: 880 880
Size: 3860
MluI A'CGCG_T
Cuts at: 3293 3293
Size: 3860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 351 2531 2715 351
Size: 2180 184 1496
Fragments arranged by size:
2180 1496 184
MscI TGG'CCA
Cuts at: 1621 1621
Size: 3860
MslI CAynn'nnrTG
Cuts at: 157 195 1849 1976 157
Size: 38 1654 127 2041
Fragments arranged by size:
2041 1654 127 38
MspA1I CmG'CkG
Cuts at: 463 540 971 1285 1645 2658 2903 463
Size: 77 431 314 360 1013 245 1420
Fragments arranged by size:
1420 1013 431 360 314 245 77
NaeI GCC'GGC
Cuts at: 2041 2041
Size: 3860
NarI GG'CG_CC
Cuts at: 140 1291 1539 140
Size: 1151 248 2461
Fragments arranged by size:
2461 1151 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3860
NgoMIV G'CCGG_C
Cuts at: 2039 2039
Size: 3860
NspI r_CATG'y
Cuts at: 483 655 666 913 1413 2320 483
Size: 172 11 247 500 907 2023
Fragments arranged by size:
2023 907 500 247 172 11
PciI A'CATG_T
Cuts at: 651 2316 651
Size: 1665 2195
PmeI GTTT'AAAC
Cuts at: 859 859
Size: 3860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3828
PspXI vC'TCGA_Gb
Cuts at: 635 635
Size: 3860
PstI C_TGCA'G
Cuts at: 907 1592 907
Size: 685 3175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3860
PvuII CAG'CTG
Cuts at: 463 540 1285 1645 463
Size: 77 745 360 2678
Fragments arranged by size:
2678 745 360 77
RsrII CG'GwC_CG
Cuts at: 2055 2055
Size: 3860
SacI G_AGCT'C
Cuts at: 406 636 874 406
Size: 230 238 3392
Fragments arranged by size:
3392 238 230
SalI G'TCGA_C
Cuts at: 897 897
Size: 3860
SapI GCTCTTCn'nnn_
Cuts at: 1883 2093 1883
Size: 210 3650
SbfI CC_TGCA'GG
Cuts at: 907 907
Size: 3860
ScaI AGT'ACT
Cuts at: 3377 3377
Size: 3860
SfcI C'TryA_G
Cuts at: 33 903 1588 2581 2772 33
Size: 870 685 993 191 1121
Fragments arranged by size:
1121 993 870 685 191
SfoI GGC'GCC
Cuts at: 141 1292 1540 141
Size: 1151 248 2461
Fragments arranged by size:
2461 1151 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
SmaI CCC'GGG
Cuts at: 882 882
Size: 3860
SmlI C'TyrA_G
Cuts at: 251 635 703 1015 1930 2422 2684 251
Size: 384 68 312 915 492 262 1427
Fragments arranged by size:
1427 915 492 384 312 262 68
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3860
SphI G_CATG'C
Cuts at: 913 1413 913
Size: 500 3360
SspI AAT'ATT
Cuts at: 184 300 184
Size: 116 3744
StyI C'CwwG_G
Cuts at: 107 994 1348 107
Size: 887 354 2619
Fragments arranged by size:
2619 887 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1443 1576 2035 2244 1443
Size: 133 459 209 3059
Fragments arranged by size:
3059 459 209 133
TatI w'GTAC_w
Cuts at: 821 3375 821
Size: 2554 1306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 3828
Tth111I GACn'n_nGTC
Cuts at: 1657 1657
Size: 3860
XbaI T'CTAG_A
Cuts at: 891 891
Size: 3860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1355 1355
Size: 3860
XhoI C'TCGA_G
Cuts at: 635 635
Size: 3860
XmaI C'CCGG_G
Cuts at: 880 880
Size: 3860
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI
ZraI