Restriction map for
pF1KB7524
(Circular) (Six-base) MAPSORT of: pF1KB7524.seq Check: 5118 from: 1 to: 3860
pF1KB7524 3860 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 898 898
Size: 3860
Acc65I G'GTAC_C
Cuts at: 876 876
Size: 3860
AclI AA'CG_TT
Cuts at: 3700 3700
Size: 3860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1685 2117 2864 3132 241
Size: 1444 432 747 268 969
Fragments arranged by size:
1444 969 747 432 268
AflIII A'CryG_T
Cuts at: 1212 2316 3293 1212
Size: 1104 977 1779
Fragments arranged by size:
1779 1104 977
AgeI A'CCGG_T
Cuts at: 2248 2248
Size: 3860
AlwNI CAG_nnn'CTG
Cuts at: 1165 2732 1165
Size: 1567 2293
ApaLI G'TGCA_C
Cuts at: 2630 2630
Size: 3860
ApoI r'AATT_y
Cuts at: 337 864 3213 3469 337
Size: 527 2349 256 728
Fragments arranged by size:
2349 728 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
AvaI C'yCGr_G
Cuts at: 806 880 806
Size: 74 3786
BamHI G'GATC_C
Cuts at: 681 885 681
Size: 204 3656
BanI G'GyrC_C
Cuts at: 876 1290 1538 1573 876
Size: 414 248 35 3163
Fragments arranged by size:
3163 414 248 35
BanII G_rGCy'C
Cuts at: 280 874 1904 280
Size: 594 1030 2236
Fragments arranged by size:
2236 1030 594
BbeI G_GCGC'C
Cuts at: 1294 1542 1294
Size: 248 3612
BciVI GTATCCnnnnn_n'
Cuts at: 1758 2519 3860 1758
Size: 761 1341 1758
Fragments arranged by size:
1758 1341 761
BclI T'GATC_A
Cuts at: 1380 1380
Size: 3860
BglII A'GATC_T
Cuts at: 1375 3412 1375
Size: 2037 1823
BlpI GC'TnA_GC
Cuts at: 972 972
Size: 3860
Bme1580I G_kGCm'C
Cuts at: 267 1485 1578 2634 267
Size: 1218 93 1056 1493
Fragments arranged by size:
1493 1218 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1072 1153 1488 3632 1072
Size: 81 335 2144 1300
Fragments arranged by size:
2144 1300 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 3860
Bpu10I CC'TnA_GC
Cuts at: 274 274
Size: 3860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 1036 1915 2407 2705 424
Size: 612 879 492 298 1579
Fragments arranged by size:
1579 879 612 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 723 3564 723
Size: 2841 1019
BsaAI yAC'GTr
Cuts at: 1213 1213
Size: 3860
BsaBI GATnn'nnATC
Cuts at: 6 1374 3411 6
Size: 1368 2037 455
Fragments arranged by size:
2037 1368 455
BsaHI Gr'CG_yC
Cuts at: 1291 1539 2241 3737 1291
Size: 248 702 1496 1414
Fragments arranged by size:
1496 1414 702 248
BsaWI w'CCGG_w
Cuts at: 1049 1570 2248 2522 2669 3080 1049
Size: 521 678 274 147 411 1829
Fragments arranged by size:
1829 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 703 733 703
Size: 30 3830
BseYI C'CCAG_C
Cuts at: 1285 2043 2620 3116 1285
Size: 758 577 496 2029
Fragments arranged by size:
2029 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 3860
BsiEI CG_ry'CG
Cuts at: 104 1402 1448 2656 104
Size: 1298 46 1208 1308
Fragments arranged by size:
1308 1298 1208 46
BsiHKAI G_wGCw'C
Cuts at: 280 537 874 1652 1842 2634 280
Size: 257 337 778 190 792 1506
Fragments arranged by size:
1506 792 778 337 257 190
Bsp1286I G_dGCh'C
Cuts at: 267 280 537 874 1485 1578 1652 1842 1904
Size: 13 257 337 611 93 74 190 62
Cuts at: 1904 2634 267
Size: 730 1493
Fragments arranged by size:
1493 730 611 337 257 190 93 74
62 13
BspEI T'CCGG_A
Cuts at: 3080 3080
Size: 3860
BspHI T'CATG_A
Cuts at: 188 3036 188
Size: 2848 1012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 910 1124 1426 1807 910
Size: 214 302 381 2963
Fragments arranged by size:
2963 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2152 2206 3690 3855 50
Size: 2102 54 1484 165 55
Fragments arranged by size:
2102 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1772 1772
Size: 3860
BsrFI r'CCGG_y
Cuts at: 1858 2039 2248 1858
Size: 181 209 3470
Fragments arranged by size:
3470 209 181
BssSI C'ACGA_G
Cuts at: 530 598 2131 2489 530
Size: 68 1533 358 1901
Fragments arranged by size:
1901 1533 358 68
BstAPI GCAn_nnn'nTGC
Cuts at: 1237 1237
Size: 3860
BstBI TT'CG_AA
Cuts at: 2221 2221
Size: 3860
BstYI r'GATC_y
Cuts at: 1 308 681 885 1352 1375 1710 1956 2968
Size: 307 373 204 467 23 335 246 1012
Cuts at: 2968 3054 3066 3412 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 373 346 335 307 246
204 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1810 1991 2154 1810
Size: 181 163 3516
Fragments arranged by size:
3516 181 163
BtsI GCAGTG_nn'
Cuts at: 651 1103 651
Size: 452 3408
DraI TTT'AAA
Cuts at: 859 859
Size: 3860
DrdI GACnn_nn'nnGTC
Cuts at: 1566 2424 1566
Size: 858 3002
EaeI y'GGCC_r
Cuts at: 242 1445 1619 2010 2037 3382 242
Size: 1203 174 391 27 1345 720
Fragments arranged by size:
1345 1203 720 391 174 27
EagI C'GGCC_G
Cuts at: 1445 1445
Size: 3860
EarI CTCTTCn'nnn_
Cuts at: 483 1883 2093 483
Size: 1400 210 2250
Fragments arranged by size:
2250 1400 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2068 2378 2524 3670 2068
Size: 310 146 1146 2258
Fragments arranged by size:
2258 1146 310 146
EcoICRI GAG'CTC
Cuts at: 278 872 278
Size: 594 3266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1685 2117 2864 3132 241
Size: 1444 432 747 268 969
Fragments arranged by size:
1444 969 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 195 216 999 195
Size: 21 783 3056
Fragments arranged by size:
3056 783 21
EcoRI G'AATT_C
Cuts at: 864 864
Size: 3860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3860
FspI TGC'GCA
Cuts at: 604 1641 604
Size: 1037 2823
FspAI rTGC'GCAy
Cuts at: 604 604
Size: 3860
HaeII r_GCGC'y
Cuts at: 1294 1542 2564 3543 1294
Size: 248 1022 979 1611
Fragments arranged by size:
1611 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1702 1732 1734 1764 1702
Size: 30 2 30 3798
Fragments arranged by size:
3798 30 30 2
HincII GTy'rAC
Cuts at: 899 899
Size: 3860
KasI G'GCGC_C
Cuts at: 1290 1538 1290
Size: 248 3612
KpnI G_GTAC'C
Cuts at: 880 880
Size: 3860
MluI A'CGCG_T
Cuts at: 3293 3293
Size: 3860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 647 2531 2715 647
Size: 1884 184 1792
Fragments arranged by size:
1884 1792 184
MscI TGG'CCA
Cuts at: 244 1621 244
Size: 1377 2483
MslI CAynn'nnrTG
Cuts at: 1849 1976 1849
Size: 127 3733
MspA1I CmG'CkG
Cuts at: 971 1285 1645 2658 2903 971
Size: 314 360 1013 245 1928
Fragments arranged by size:
1928 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2041 2041
Size: 3860
NarI GG'CG_CC
Cuts at: 1291 1539 1291
Size: 248 3612
NcoI C'CATG_G
Cuts at: 107 107
Size: 3860
NgoMIV G'CCGG_C
Cuts at: 2039 2039
Size: 3860
NspI r_CATG'y
Cuts at: 913 1413 2320 913
Size: 500 907 2453
Fragments arranged by size:
2453 907 500
PciI A'CATG_T
Cuts at: 2316 2316
Size: 3860
PflMI CCAn_nnn'nTGG
Cuts at: 767 767
Size: 3860
PfoI T'CCnGG_A
Cuts at: 311 311
Size: 3860
PmeI GTTT'AAAC
Cuts at: 859 859
Size: 3860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3828
PpuMI rG'GwC_Cy
Cuts at: 195 216 195
Size: 21 3839
PstI C_TGCA'G
Cuts at: 907 1592 907
Size: 685 3175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3860
PvuII CAG'CTG
Cuts at: 1285 1645 1285
Size: 360 3500
RsrII CG'GwC_CG
Cuts at: 2055 2055
Size: 3860
SacI G_AGCT'C
Cuts at: 280 874 280
Size: 594 3266
SalI G'TCGA_C
Cuts at: 897 897
Size: 3860
SapI GCTCTTCn'nnn_
Cuts at: 483 1883 2093 483
Size: 1400 210 2250
Fragments arranged by size:
2250 1400 210
SbfI CC_TGCA'GG
Cuts at: 907 907
Size: 3860
ScaI AGT'ACT
Cuts at: 3377 3377
Size: 3860
SexAI A'CCwGG_T
Cuts at: 713 713
Size: 3860
SfcI C'TryA_G
Cuts at: 33 903 1588 2581 2772 33
Size: 870 685 993 191 1121
Fragments arranged by size:
1121 993 870 685 191
SfoI GGC'GCC
Cuts at: 1292 1540 1292
Size: 248 3612
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
SmaI CCC'GGG
Cuts at: 882 882
Size: 3860
SmlI C'TyrA_G
Cuts at: 439 1015 1930 2422 2684 439
Size: 576 915 492 262 1615
Fragments arranged by size:
1615 915 576 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3860
SphI G_CATG'C
Cuts at: 913 1413 913
Size: 500 3360
StyI C'CwwG_G
Cuts at: 107 994 1348 107
Size: 887 354 2619
Fragments arranged by size:
2619 887 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1443 1576 2035 2244 1443
Size: 133 459 209 3059
Fragments arranged by size:
3059 459 209 133
TatI w'GTAC_w
Cuts at: 3375 3375
Size: 3860
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 703 735 3377 3409 703
Size: 32 2642 32 1154
Fragments arranged by size:
2642 1154 32 32
Tth111I GACn'n_nGTC
Cuts at: 1657 1657
Size: 3860
XbaI T'CTAG_A
Cuts at: 891 891
Size: 3860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 319 721 1355 319
Size: 402 634 2824
Fragments arranged by size:
2824 634 402
XmaI C'CCGG_G
Cuts at: 880 880
Size: 3860
XmnI GAAnn'nnTTC
Cuts at: 155 155
Size: 3860
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI