Restriction map for
pF1KB7525
(Circular) (Six-base) MAPSORT of: pF1KB7525.seq Check: 5267 from: 1 to: 3563
pF1KB7525 3563 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 257 601 257
Size: 344 3219
Acc65I G'GTAC_C
Cuts at: 310 579 310
Size: 269 3294
AclI AA'CG_TT
Cuts at: 3403 3403
Size: 3563
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 1388 1820 2567 2835 446
Size: 942 432 747 268 1174
Fragments arranged by size:
1174 942 747 432 268
AflIII A'CryG_T
Cuts at: 452 915 2019 2996 452
Size: 463 1104 977 1019
Fragments arranged by size:
1104 1019 977 463
AgeI A'CCGG_T
Cuts at: 1951 1951
Size: 3563
AhdI GACnn_n'nnGTC
Cuts at: 180 180
Size: 3563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 506 506
Size: 3563
AlwNI CAG_nnn'CTG
Cuts at: 868 2435 868
Size: 1567 1996
ApaLI G'TGCA_C
Cuts at: 2333 2333
Size: 3563
ApoI r'AATT_y
Cuts at: 245 567 2916 3172 245
Size: 322 2349 256 636
Fragments arranged by size:
2349 636 322 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3563
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3563
AvaI C'yCGr_G
Cuts at: 583 583
Size: 3563
BamHI G'GATC_C
Cuts at: 588 588
Size: 3563
BanI G'GyrC_C
Cuts at: 310 579 993 1241 1276 310
Size: 269 414 248 35 2597
Fragments arranged by size:
2597 414 269 248 35
BanII G_rGCy'C
Cuts at: 577 1607 577
Size: 1030 2533
BbeI G_GCGC'C
Cuts at: 997 1245 997
Size: 248 3315
BciVI GTATCCnnnnn_n'
Cuts at: 1461 2222 3563 1461
Size: 761 1341 1461
Fragments arranged by size:
1461 1341 761
BclI T'GATC_A
Cuts at: 1083 1083
Size: 3563
BglII A'GATC_T
Cuts at: 1078 3115 1078
Size: 2037 1526
BlpI GC'TnA_GC
Cuts at: 675 675
Size: 3563
Bme1580I G_kGCm'C
Cuts at: 1188 1281 2337 1188
Size: 93 1056 2414
Fragments arranged by size:
2414 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 775 856 1191 3335 775
Size: 81 335 2144 1003
Fragments arranged by size:
2144 1003 335 81
BmtI G_CTAG'C
Cuts at: 553 553
Size: 3563
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 537 550 1930 537
Size: 13 1380 2170
Fragments arranged by size:
2170 1380 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 507
Size: 3563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1618 2110 2408 739
Size: 879 492 298 1894
Fragments arranged by size:
1894 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3267 3267
Size: 3563
BsaAI yAC'GTr
Cuts at: 453 916 453
Size: 463 3100
BsaBI GATnn'nnATC
Cuts at: 6 1077 3114 6
Size: 1071 2037 455
Fragments arranged by size:
2037 1071 455
BsaHI Gr'CG_yC
Cuts at: 994 1242 1944 3440 994
Size: 248 702 1496 1117
Fragments arranged by size:
1496 1117 702 248
BsaWI w'CCGG_w
Cuts at: 752 1273 1951 2225 2372 2783 752
Size: 521 678 274 147 411 1532
Fragments arranged by size:
1532 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 988 1746 2323 2819 988
Size: 758 577 496 1732
Fragments arranged by size:
1732 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1105 1151 2359 104
Size: 1001 46 1208 1308
Fragments arranged by size:
1308 1208 1001 46
BsiHKAI G_wGCw'C
Cuts at: 577 1355 1545 2337 577
Size: 778 190 792 1803
Fragments arranged by size:
1803 792 778 190
BsiWI C'GTAC_G
Cuts at: 449 449
Size: 3563
Bsp1286I G_dGCh'C
Cuts at: 577 1188 1281 1355 1545 1607 2337 577
Size: 611 93 74 190 62 730 1803
Fragments arranged by size:
1803 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2783 2783
Size: 3563
BspHI T'CATG_A
Cuts at: 473 2739 473
Size: 2266 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 613 827 1129 1510 509
Size: 104 214 302 381 2562
Fragments arranged by size:
2562 381 302 214 104
BsrBI CCG'CTC
Cuts at: 50 1855 1909 3393 3558 50
Size: 1805 54 1484 165 55
Fragments arranged by size:
1805 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1475 1475
Size: 3563
BsrFI r'CCGG_y
Cuts at: 1561 1742 1951 1561
Size: 181 209 3173
Fragments arranged by size:
3173 209 181
BsrGI T'GTAC_A
Cuts at: 395 395
Size: 3563
BssSI C'ACGA_G
Cuts at: 1834 2192 1834
Size: 358 3205
BstAPI GCAn_nnn'nTGC
Cuts at: 940 940
Size: 3563
BstBI TT'CG_AA
Cuts at: 1924 1924
Size: 3563
BstYI r'GATC_y
Cuts at: 1 219 588 1055 1078 1413 1659 2671 2757
Size: 218 369 467 23 335 246 1012 86
Cuts at: 2757 2769 3115 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 369 346 335 246 218
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1513 1694 1857 1513
Size: 181 163 3219
Fragments arranged by size:
3219 181 163
BtsI GCAGTG_nn'
Cuts at: 806 806
Size: 3563
DraI TTT'AAA
Cuts at: 562 562
Size: 3563
DrdI GACnn_nn'nnGTC
Cuts at: 1269 2127 1269
Size: 858 2705
EaeI y'GGCC_r
Cuts at: 1148 1322 1713 1740 3085 1148
Size: 174 391 27 1345 1626
Fragments arranged by size:
1626 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1148 1148
Size: 3563
EarI CTCTTCn'nnn_
Cuts at: 1586 1796 1586
Size: 210 3353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1771 2081 2227 3373 1771
Size: 310 146 1146 1961
Fragments arranged by size:
1961 1146 310 146
EcoICRI GAG'CTC
Cuts at: 575 575
Size: 3563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 507 1388 1820 2567 2835 446
Size: 61 881 432 747 268 1174
Fragments arranged by size:
1174 881 747 432 268 61
EcoNI CCTnn'n_nnAGG
Cuts at: 124 124
Size: 3563
EcoO109I rG'GnC_Cy
Cuts at: 465 702 465
Size: 237 3326
EcoRI G'AATT_C
Cuts at: 567 567
Size: 3563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 570 1582 570
Size: 1012 2551
FspI TGC'GCA
Cuts at: 1344 1344
Size: 3563
HaeII r_GCGC'y
Cuts at: 997 1245 2267 3246 997
Size: 248 1022 979 1314
Fragments arranged by size:
1314 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 190 198 222 1405 1435 1437 1467 166
Size: 24 8 24 1183 30 2 30 2262
Fragments arranged by size:
2262 1183 30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 602 602
Size: 3563
KasI G'GCGC_C
Cuts at: 993 1241 993
Size: 248 3315
KpnI G_GTAC'C
Cuts at: 314 583 314
Size: 269 3294
MluI A'CGCG_T
Cuts at: 2996 2996
Size: 3563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2418 2234
Size: 184 3379
MscI TGG'CCA
Cuts at: 1324 1324
Size: 3563
MslI CAynn'nnrTG
Cuts at: 1552 1679 1552
Size: 127 3436
MspA1I CmG'CkG
Cuts at: 674 988 1348 2361 2606 674
Size: 314 360 1013 245 1631
Fragments arranged by size:
1631 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1744 1744
Size: 3563
NarI GG'CG_CC
Cuts at: 994 1242 994
Size: 248 3315
NcoI C'CATG_G
Cuts at: 107 107
Size: 3563
NgoMIV G'CCGG_C
Cuts at: 1742 1742
Size: 3563
NheI G'CTAG_C
Cuts at: 549 549
Size: 3563
NspI r_CATG'y
Cuts at: 616 1116 2023 616
Size: 500 907 2156
Fragments arranged by size:
2156 907 500
PciI A'CATG_T
Cuts at: 2019 2019
Size: 3563
PmeI GTTT'AAAC
Cuts at: 562 562
Size: 3563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2702 2734 2702
Size: 32 3531
PstI C_TGCA'G
Cuts at: 485 610 1295 485
Size: 125 685 2753
Fragments arranged by size:
2753 685 125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3563
PvuII CAG'CTG
Cuts at: 988 1348 988
Size: 360 3203
RsrII CG'GwC_CG
Cuts at: 1758 1758
Size: 3563
SacI G_AGCT'C
Cuts at: 577 577
Size: 3563
SalI G'TCGA_C
Cuts at: 600 600
Size: 3563
SapI GCTCTTCn'nnn_
Cuts at: 1586 1796 1586
Size: 210 3353
SbfI CC_TGCA'GG
Cuts at: 610 610
Size: 3563
ScaI AGT'ACT
Cuts at: 3080 3080
Size: 3563
SfcI C'TryA_G
Cuts at: 33 254 481 606 1291 2284 2475 33
Size: 221 227 125 685 993 191 1121
Fragments arranged by size:
1121 993 685 227 221 191 125
SfoI GGC'GCC
Cuts at: 995 1243 995
Size: 248 3315
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3563
SmaI CCC'GGG
Cuts at: 585 585
Size: 3563
SmlI C'TyrA_G
Cuts at: 718 1633 2125 2387 718
Size: 915 492 262 1894
Fragments arranged by size:
1894 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3563
SphI G_CATG'C
Cuts at: 616 1116 616
Size: 500 3063
StuI AGG'CCT
Cuts at: 351 351
Size: 3563
StyI C'CwwG_G
Cuts at: 107 697 1051 107
Size: 590 354 2619
Fragments arranged by size:
2619 590 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 191 253 1146 1279 1738 1947 191
Size: 62 893 133 459 209 1807
Fragments arranged by size:
1807 893 459 209 133 62
TatI w'GTAC_w
Cuts at: 395 3078 395
Size: 2683 880
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 500 532 3080 3112 500
Size: 32 2548 32 951
Fragments arranged by size:
2548 951 32 32
Tth111I GACn'n_nGTC
Cuts at: 1360 1360
Size: 3563
XbaI T'CTAG_A
Cuts at: 594 594
Size: 3563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1058 1058
Size: 3563
XmaI C'CCGG_G
Cuts at: 583 583
Size: 3563
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
Bpu10I BsaXI BseRI BsgI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI