Restriction map for
pF1KB7527
(Circular) (Six-base) MAPSORT of: pF1KB7527.seq Check: 8505 from: 1 to: 3578
pF1KB7527 3578 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 616 616
Size: 3578
Acc65I G'GTAC_C
Cuts at: 594 594
Size: 3578
AclI AA'CG_TT
Cuts at: 3418 3418
Size: 3578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1403 1835 2582 2850 1403
Size: 432 747 268 2131
Fragments arranged by size:
2131 747 432 268
AflIII A'CryG_T
Cuts at: 930 2034 3011 930
Size: 1104 977 1497
Fragments arranged by size:
1497 1104 977
AgeI A'CCGG_T
Cuts at: 1966 1966
Size: 3578
AlwNI CAG_nnn'CTG
Cuts at: 883 2450 883
Size: 1567 2011
ApaLI G'TGCA_C
Cuts at: 2348 2348
Size: 3578
ApoI r'AATT_y
Cuts at: 378 387 582 2931 3187 378
Size: 9 195 2349 256 769
Fragments arranged by size:
2349 769 256 195 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 3578
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
AvaI C'yCGr_G
Cuts at: 598 598
Size: 3578
BamHI G'GATC_C
Cuts at: 603 603
Size: 3578
BanI G'GyrC_C
Cuts at: 594 1008 1256 1291 594
Size: 414 248 35 2881
Fragments arranged by size:
2881 414 248 35
BanII G_rGCy'C
Cuts at: 592 1622 592
Size: 1030 2548
BbeI G_GCGC'C
Cuts at: 1012 1260 1012
Size: 248 3330
BbsI GAAGACnn'nnnn_
Cuts at: 365 476 365
Size: 111 3467
BciVI GTATCCnnnnn_n'
Cuts at: 1476 2237 3578 1476
Size: 761 1341 1476
Fragments arranged by size:
1476 1341 761
BclI T'GATC_A
Cuts at: 1098 1098
Size: 3578
BglII A'GATC_T
Cuts at: 1093 3130 1093
Size: 2037 1541
BlpI GC'TnA_GC
Cuts at: 690 690
Size: 3578
Bme1580I G_kGCm'C
Cuts at: 191 1203 1296 2352 191
Size: 1012 93 1056 1417
Fragments arranged by size:
1417 1056 1012 93
BmrI ACTGGGnnnn_n'
Cuts at: 790 871 1206 3350 790
Size: 81 335 2144 1018
Fragments arranged by size:
2144 1018 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1633 2125 2423 754
Size: 879 492 298 1909
Fragments arranged by size:
1909 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3282 3282
Size: 3578
BsaAI yAC'GTr
Cuts at: 931 931
Size: 3578
BsaBI GATnn'nnATC
Cuts at: 6 1092 3129 6
Size: 1086 2037 455
Fragments arranged by size:
2037 1086 455
BsaHI Gr'CG_yC
Cuts at: 112 1009 1257 1959 3455 112
Size: 897 248 702 1496 235
Fragments arranged by size:
1496 897 702 248 235
BsaWI w'CCGG_w
Cuts at: 212 767 1288 1966 2240 2387 2798 212
Size: 555 521 678 274 147 411 992
Fragments arranged by size:
992 678 555 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 580
Size: 3578
BseYI C'CCAG_C
Cuts at: 1003 1761 2338 2834 1003
Size: 758 577 496 1747
Fragments arranged by size:
1747 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1120 1166 2374 104
Size: 1016 46 1208 1308
Fragments arranged by size:
1308 1208 1016 46
BsiHKAI G_wGCw'C
Cuts at: 592 1370 1560 2352 592
Size: 778 190 792 1818
Fragments arranged by size:
1818 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 191 592 1203 1296 1370 1560 1622 2352 191
Size: 401 611 93 74 190 62 730 1417
Fragments arranged by size:
1417 730 611 401 190 93 74 62
BspEI T'CCGG_A
Cuts at: 212 2798 212
Size: 2586 992
BspHI T'CATG_A
Cuts at: 485 2754 485
Size: 2269 1309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 842 1144 1525 628
Size: 214 302 381 2681
Fragments arranged by size:
2681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1870 1924 3408 3573 50
Size: 1820 54 1484 165 55
Fragments arranged by size:
1820 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1490 1490
Size: 3578
BsrFI r'CCGG_y
Cuts at: 1576 1757 1966 1576
Size: 181 209 3188
Fragments arranged by size:
3188 209 181
BssSI C'ACGA_G
Cuts at: 1849 2207 1849
Size: 358 3220
BstAPI GCAn_nnn'nTGC
Cuts at: 955 955
Size: 3578
BstBI TT'CG_AA
Cuts at: 1939 1939
Size: 3578
BstYI r'GATC_y
Cuts at: 1 603 1070 1093 1428 1674 2686 2772 2784
Size: 602 467 23 335 246 1012 86 12
Cuts at: 2784 3130 1
Size: 346 449
Fragments arranged by size:
1012 602 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 436 436
Size: 3578
BtgI C'CryG_G
Cuts at: 107 107
Size: 3578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1528 1709 1872 1528
Size: 181 163 3234
Fragments arranged by size:
3234 181 163
BtsI GCAGTG_nn'
Cuts at: 821 821
Size: 3578
DraI TTT'AAA
Cuts at: 383 577 383
Size: 194 3384
DrdI GACnn_nn'nnGTC
Cuts at: 1284 2142 1284
Size: 858 2720
EaeI y'GGCC_r
Cuts at: 1163 1337 1728 1755 3100 1163
Size: 174 391 27 1345 1641
Fragments arranged by size:
1641 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1163 1163
Size: 3578
EarI CTCTTCn'nnn_
Cuts at: 281 1601 1811 281
Size: 1320 210 2048
Fragments arranged by size:
2048 1320 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1786 2096 2242 3388 1786
Size: 310 146 1146 1976
Fragments arranged by size:
1976 1146 310 146
EcoICRI GAG'CTC
Cuts at: 590 590
Size: 3578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1403 1835 2582 2850 1403
Size: 432 747 268 2131
Fragments arranged by size:
2131 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 460 460
Size: 3578
EcoO109I rG'GnC_Cy
Cuts at: 717 717
Size: 3578
EcoRI G'AATT_C
Cuts at: 582 582
Size: 3578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1597 1597
Size: 3578
FspI TGC'GCA
Cuts at: 1359 1359
Size: 3578
HaeII r_GCGC'y
Cuts at: 1012 1260 2282 3261 1012
Size: 248 1022 979 1329
Fragments arranged by size:
1329 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 307 339 1420 1450 1452 1482 307
Size: 32 1081 30 2 30 2403
Fragments arranged by size:
2403 1081 32 30 30 2
HincII GTy'rAC
Cuts at: 453 617 453
Size: 164 3414
HindIII A'AGCT_T
Cuts at: 414 414
Size: 3578
KasI G'GCGC_C
Cuts at: 1008 1256 1008
Size: 248 3330
KpnI G_GTAC'C
Cuts at: 598 598
Size: 3578
MluI A'CGCG_T
Cuts at: 3011 3011
Size: 3578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2433 2249
Size: 184 3394
MscI TGG'CCA
Cuts at: 1339 1339
Size: 3578
MslI CAynn'nnrTG
Cuts at: 1567 1694 1567
Size: 127 3451
MspA1I CmG'CkG
Cuts at: 170 689 1003 1363 2376 2621 170
Size: 519 314 360 1013 245 1127
Fragments arranged by size:
1127 1013 519 360 314 245
NaeI GCC'GGC
Cuts at: 1759 1759
Size: 3578
NarI GG'CG_CC
Cuts at: 1009 1257 1009
Size: 248 3330
NcoI C'CATG_G
Cuts at: 107 107
Size: 3578
NgoMIV G'CCGG_C
Cuts at: 1757 1757
Size: 3578
NspI r_CATG'y
Cuts at: 501 631 1131 2038 501
Size: 130 500 907 2041
Fragments arranged by size:
2041 907 500 130
PciI A'CATG_T
Cuts at: 2034 2034
Size: 3578
PmeI GTTT'AAAC
Cuts at: 577 577
Size: 3578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 2717
Size: 32 3546
PstI C_TGCA'G
Cuts at: 625 1310 625
Size: 685 2893
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3578
PvuII CAG'CTG
Cuts at: 1003 1363 1003
Size: 360 3218
RsrII CG'GwC_CG
Cuts at: 1773 1773
Size: 3578
SacI G_AGCT'C
Cuts at: 592 592
Size: 3578
SalI G'TCGA_C
Cuts at: 615 615
Size: 3578
SapI GCTCTTCn'nnn_
Cuts at: 281 1601 1811 281
Size: 1320 210 2048
Fragments arranged by size:
2048 1320 210
SbfI CC_TGCA'GG
Cuts at: 625 625
Size: 3578
ScaI AGT'ACT
Cuts at: 3095 3095
Size: 3578
SfcI C'TryA_G
Cuts at: 33 326 621 1306 2299 2490 33
Size: 293 295 685 993 191 1121
Fragments arranged by size:
1121 993 685 295 293 191
SfoI GGC'GCC
Cuts at: 1010 1258 1010
Size: 248 3330
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
SmaI CCC'GGG
Cuts at: 600 600
Size: 3578
SmlI C'TyrA_G
Cuts at: 733 1648 2140 2402 733
Size: 915 492 262 1909
Fragments arranged by size:
1909 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3578
SphI G_CATG'C
Cuts at: 631 1131 631
Size: 500 3078
StyI C'CwwG_G
Cuts at: 107 712 1066 107
Size: 605 354 2619
Fragments arranged by size:
2619 605 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1161 1294 1753 1962 1161
Size: 133 459 209 2777
Fragments arranged by size:
2777 459 209 133
TatI w'GTAC_w
Cuts at: 3093 3093
Size: 3578
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3546
Tth111I GACn'n_nGTC
Cuts at: 1375 1375
Size: 3578
XbaI T'CTAG_A
Cuts at: 609 609
Size: 3578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1073 1073
Size: 3578
XmaI C'CCGG_G
Cuts at: 598 598
Size: 3578
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI