Restriction map for
pF1KB7529
(Circular) (Six-base) MAPSORT of: pF1KB7529.seq Check: 5401 from: 1 to: 3602
pF1KB7529 3602 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 529 529
Size: 3602
AccI GT'mk_AC
Cuts at: 640 640
Size: 3602
Acc65I G'GTAC_C
Cuts at: 618 618
Size: 3602
AclI AA'CG_TT
Cuts at: 3442 3442
Size: 3602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 490 1427 1859 2606 2874 469
Size: 21 937 432 747 268 1197
Fragments arranged by size:
1197 937 747 432 268 21
AflIII A'CryG_T
Cuts at: 954 2058 3035 954
Size: 1104 977 1521
Fragments arranged by size:
1521 1104 977
AgeI A'CCGG_T
Cuts at: 1990 1990
Size: 3602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 387 589 387
Size: 202 3400
AlwNI CAG_nnn'CTG
Cuts at: 907 2474 907
Size: 1567 2035
ApaLI G'TGCA_C
Cuts at: 2372 2372
Size: 3602
ApoI r'AATT_y
Cuts at: 606 2955 3211 606
Size: 2349 256 997
Fragments arranged by size:
2349 997 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3602
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
AvaI C'yCGr_G
Cuts at: 235 553 577 586 622 235
Size: 318 24 9 36 3215
Fragments arranged by size:
3215 318 36 24 9
BamHI G'GATC_C
Cuts at: 627 627
Size: 3602
BanI G'GyrC_C
Cuts at: 338 618 1032 1280 1315 338
Size: 280 414 248 35 2625
Fragments arranged by size:
2625 414 280 248 35
BanII G_rGCy'C
Cuts at: 182 616 1646 182
Size: 434 1030 2138
Fragments arranged by size:
2138 1030 434
BbeI G_GCGC'C
Cuts at: 342 1036 1284 342
Size: 694 248 2660
Fragments arranged by size:
2660 694 248
BbsI GAAGACnn'nnnn_
Cuts at: 457 457
Size: 3602
BciVI GTATCCnnnnn_n'
Cuts at: 1500 2261 3602 1500
Size: 761 1341 1500
Fragments arranged by size:
1500 1341 761
BclI T'GATC_A
Cuts at: 1122 1122
Size: 3602
BglII A'GATC_T
Cuts at: 1117 3154 1117
Size: 2037 1565
BlpI GC'TnA_GC
Cuts at: 714 714
Size: 3602
Bme1580I G_kGCm'C
Cuts at: 1227 1320 2376 1227
Size: 93 1056 2453
Fragments arranged by size:
2453 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 286 814 895 1230 3374 286
Size: 528 81 335 2144 514
Fragments arranged by size:
2144 528 514 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 286
Size: 3602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1657 2149 2447 778
Size: 879 492 298 1933
Fragments arranged by size:
1933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3306 3306
Size: 3602
BsaAI yAC'GTr
Cuts at: 955 955
Size: 3602
BsaBI GATnn'nnATC
Cuts at: 6 1116 3153 6
Size: 1110 2037 455
Fragments arranged by size:
2037 1110 455
BsaHI Gr'CG_yC
Cuts at: 339 1033 1281 1983 3479 339
Size: 694 248 702 1496 462
Fragments arranged by size:
1496 702 694 462 248
BsaWI w'CCGG_w
Cuts at: 530 791 1312 1990 2264 2411 2822 530
Size: 261 521 678 274 147 411 1310
Fragments arranged by size:
1310 678 521 411 274 261 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 337 478 220
Size: 117 141 3344
Fragments arranged by size:
3344 141 117
BseYI C'CCAG_C
Cuts at: 184 290 1027 1785 2362 2858 184
Size: 106 737 758 577 496 928
Fragments arranged by size:
928 758 737 577 496 106
BsiEI CG_ry'CG
Cuts at: 104 1144 1190 2398 104
Size: 1040 46 1208 1308
Fragments arranged by size:
1308 1208 1040 46
BsiHKAI G_wGCw'C
Cuts at: 616 1394 1584 2376 616
Size: 778 190 792 1842
Fragments arranged by size:
1842 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 182 616 1227 1320 1394 1584 1646 2376 182
Size: 434 611 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 434 190 93 74 62
BspEI T'CCGG_A
Cuts at: 530 2822 530
Size: 2292 1310
BspHI T'CATG_A
Cuts at: 434 2778 434
Size: 2344 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 529 652 866 1168 1549 529
Size: 123 214 302 381 2582
Fragments arranged by size:
2582 381 302 214 123
BsrBI CCG'CTC
Cuts at: 50 423 480 1894 1948 3432 3597 50
Size: 373 57 1414 54 1484 165 55
Fragments arranged by size:
1484 1414 373 165 57 55 54
BsrDI GCAATG_nn'
Cuts at: 1514 1514
Size: 3602
BsrFI r'CCGG_y
Cuts at: 1600 1781 1990 1600
Size: 181 209 3212
Fragments arranged by size:
3212 209 181
BssSI C'ACGA_G
Cuts at: 1873 2231 1873
Size: 358 3244
BstAPI GCAn_nnn'nTGC
Cuts at: 979 979
Size: 3602
BstBI TT'CG_AA
Cuts at: 1963 1963
Size: 3602
BstEII G'GTnAC_C
Cuts at: 138 138
Size: 3602
BstYI r'GATC_y
Cuts at: 1 122 627 1094 1117 1452 1698 2710 2796
Size: 121 505 467 23 335 246 1012 86
Cuts at: 2796 2808 3154 1
Size: 12 346 449
Fragments arranged by size:
1012 505 467 449 346 335 246 121
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1552 1733 1896 1552
Size: 181 163 3258
Fragments arranged by size:
3258 181 163
BtsI GCAGTG_nn'
Cuts at: 571 845 571
Size: 274 3328
DraI TTT'AAA
Cuts at: 601 601
Size: 3602
DrdI GACnn_nn'nnGTC
Cuts at: 555 1308 2166 555
Size: 753 858 1991
Fragments arranged by size:
1991 858 753
EaeI y'GGCC_r
Cuts at: 1187 1361 1752 1779 3124 1187
Size: 174 391 27 1345 1665
Fragments arranged by size:
1665 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1187 1187
Size: 3602
EarI CTCTTCn'nnn_
Cuts at: 314 1625 1835 314
Size: 1311 210 2081
Fragments arranged by size:
2081 1311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1810 2120 2266 3412 1810
Size: 310 146 1146 2000
Fragments arranged by size:
2000 1146 310 146
EcoICRI GAG'CTC
Cuts at: 614 614
Size: 3602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 469 490 1427 1859 2606 2874 286
Size: 183 21 937 432 747 268 1014
Fragments arranged by size:
1014 937 747 432 268 183 21
EcoO109I rG'GnC_Cy
Cuts at: 287 741 287
Size: 454 3148
EcoRI G'AATT_C
Cuts at: 606 606
Size: 3602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1621 1621
Size: 3602
FspI TGC'GCA
Cuts at: 1383 1383
Size: 3602
HaeII r_GCGC'y
Cuts at: 342 1036 1284 2306 3285 342
Size: 694 248 1022 979 659
Fragments arranged by size:
1022 979 694 659 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1444 1474 1476 1506 1444
Size: 30 2 30 3540
Fragments arranged by size:
3540 30 30 2
HincII GTy'rAC
Cuts at: 641 641
Size: 3602
KasI G'GCGC_C
Cuts at: 338 1032 1280 338
Size: 694 248 2660
Fragments arranged by size:
2660 694 248
KpnI G_GTAC'C
Cuts at: 622 622
Size: 3602
MluI A'CGCG_T
Cuts at: 3035 3035
Size: 3602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2457 2273
Size: 184 3418
MscI TGG'CCA
Cuts at: 1363 1363
Size: 3602
MslI CAynn'nnrTG
Cuts at: 496 1591 1718 496
Size: 1095 127 2380
Fragments arranged by size:
2380 1095 127
MspA1I CmG'CkG
Cuts at: 411 713 1027 1387 2400 2645 411
Size: 302 314 360 1013 245 1368
Fragments arranged by size:
1368 1013 360 314 302 245
NaeI GCC'GGC
Cuts at: 1783 1783
Size: 3602
NarI GG'CG_CC
Cuts at: 339 1033 1281 339
Size: 694 248 2660
Fragments arranged by size:
2660 694 248
NgoMIV G'CCGG_C
Cuts at: 1781 1781
Size: 3602
NspI r_CATG'y
Cuts at: 655 1155 2062 655
Size: 500 907 2195
Fragments arranged by size:
2195 907 500
PciI A'CATG_T
Cuts at: 2058 2058
Size: 3602
PflMI CCAn_nnn'nTGG
Cuts at: 163 163
Size: 3602
PfoI T'CCnGG_A
Cuts at: 125 263 125
Size: 138 3464
PmeI GTTT'AAAC
Cuts at: 601 601
Size: 3602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 3570
PspXI vC'TCGA_Gb
Cuts at: 577 577
Size: 3602
PstI C_TGCA'G
Cuts at: 410 649 1334 410
Size: 239 685 2678
Fragments arranged by size:
2678 685 239
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3602
PvuII CAG'CTG
Cuts at: 1027 1387 1027
Size: 360 3242
RsrII CG'GwC_CG
Cuts at: 533 1797 533
Size: 1264 2338
SacI G_AGCT'C
Cuts at: 616 616
Size: 3602
SalI G'TCGA_C
Cuts at: 639 639
Size: 3602
SapI GCTCTTCn'nnn_
Cuts at: 1625 1835 1625
Size: 210 3392
SbfI CC_TGCA'GG
Cuts at: 649 649
Size: 3602
ScaI AGT'ACT
Cuts at: 3119 3119
Size: 3602
SfcI C'TryA_G
Cuts at: 33 406 645 1330 2323 2514 33
Size: 373 239 685 993 191 1121
Fragments arranged by size:
1121 993 685 373 239 191
SfoI GGC'GCC
Cuts at: 340 1034 1282 340
Size: 694 248 2660
Fragments arranged by size:
2660 694 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
SmaI CCC'GGG
Cuts at: 624 624
Size: 3602
SmlI C'TyrA_G
Cuts at: 577 586 757 1672 2164 2426 577
Size: 9 171 915 492 262 1753
Fragments arranged by size:
1753 915 492 262 171 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3602
SphI G_CATG'C
Cuts at: 655 1155 655
Size: 500 3102
StyI C'CwwG_G
Cuts at: 736 1090 736
Size: 354 3248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 550 1185 1318 1777 1986 550
Size: 635 133 459 209 2166
Fragments arranged by size:
2166 635 459 209 133
TatI w'GTAC_w
Cuts at: 3117 3117
Size: 3602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 525 557 3119 3151 525
Size: 32 2562 32 976
Fragments arranged by size:
2562 976 32 32
Tth111I GACn'n_nGTC
Cuts at: 541 1399 541
Size: 858 2744
XbaI T'CTAG_A
Cuts at: 633 633
Size: 3602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1097 1097
Size: 3602
XhoI C'TCGA_G
Cuts at: 577 586 577
Size: 9 3593
XmaI C'CCGG_G
Cuts at: 622 622
Size: 3602
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI