Restriction map for
pF1KB7530
(Circular) (Six-base) MAPSORT of: pF1KB7530.seq Check: 9448 from: 1 to: 3878
pF1KB7530 3878 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 519 519
Size: 3878
AccI GT'mk_AC
Cuts at: 916 916
Size: 3878
Acc65I G'GTAC_C
Cuts at: 894 894
Size: 3878
AclI AA'CG_TT
Cuts at: 3718 3718
Size: 3878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 1703 2135 2882 3150 415
Size: 1288 432 747 268 1143
Fragments arranged by size:
1288 1143 747 432 268
AflIII A'CryG_T
Cuts at: 1230 2334 3311 1230
Size: 1104 977 1797
Fragments arranged by size:
1797 1104 977
AgeI A'CCGG_T
Cuts at: 2266 2266
Size: 3878
AlwNI CAG_nnn'CTG
Cuts at: 459 786 1183 2750 459
Size: 327 397 1567 1587
Fragments arranged by size:
1587 1567 397 327
ApaLI G'TGCA_C
Cuts at: 2648 2648
Size: 3878
ApoI r'AATT_y
Cuts at: 187 882 3231 3487 187
Size: 695 2349 256 578
Fragments arranged by size:
2349 695 578 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
AvaI C'yCGr_G
Cuts at: 178 898 178
Size: 720 3158
BamHI G'GATC_C
Cuts at: 903 903
Size: 3878
BanI G'GyrC_C
Cuts at: 389 894 1308 1556 1591 389
Size: 505 414 248 35 2676
Fragments arranged by size:
2676 505 414 248 35
BanII G_rGCy'C
Cuts at: 253 575 892 1922 253
Size: 322 317 1030 2209
Fragments arranged by size:
2209 1030 322 317
BbeI G_GCGC'C
Cuts at: 393 1312 1560 393
Size: 919 248 2711
Fragments arranged by size:
2711 919 248
BciVI GTATCCnnnnn_n'
Cuts at: 1776 2537 3878 1776
Size: 761 1341 1776
Fragments arranged by size:
1776 1341 761
BclI T'GATC_A
Cuts at: 1398 1398
Size: 3878
BglII A'GATC_T
Cuts at: 1393 3430 1393
Size: 2037 1841
BlpI GC'TnA_GC
Cuts at: 990 990
Size: 3878
Bme1580I G_kGCm'C
Cuts at: 1503 1596 2652 1503
Size: 93 1056 2729
Fragments arranged by size:
2729 1056 93
BmgBI CAC'GTC
Cuts at: 114 114
Size: 3878
BmrI ACTGGGnnnn_n'
Cuts at: 1090 1171 1506 3650 1090
Size: 81 335 2144 1318
Fragments arranged by size:
2144 1318 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 3878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 785 818 763
Size: 22 33 3823
Fragments arranged by size:
3823 33 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 1054 1933 2425 2723 409
Size: 645 879 492 298 1564
Fragments arranged by size:
1564 879 645 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3582 3582
Size: 3878
BsaAI yAC'GTr
Cuts at: 270 1231 270
Size: 961 2917
BsaBI GATnn'nnATC
Cuts at: 6 1392 3429 6
Size: 1386 2037 455
Fragments arranged by size:
2037 1386 455
BsaHI Gr'CG_yC
Cuts at: 390 1309 1557 2259 3755 390
Size: 919 248 702 1496 513
Fragments arranged by size:
1496 919 702 513 248
BsaWI w'CCGG_w
Cuts at: 743 1067 1588 2266 2540 2687 3098 743
Size: 324 521 678 274 147 411 1523
Fragments arranged by size:
1523 678 521 411 324 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 145
Size: 30 3848
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 744 744
Size: 3878
BseYI C'CCAG_C
Cuts at: 1303 2061 2638 3134 1303
Size: 758 577 496 2047
Fragments arranged by size:
2047 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1420 1466 2674 104
Size: 1316 46 1208 1308
Fragments arranged by size:
1316 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 269 892 1670 1860 2652 269
Size: 623 778 190 792 1495
Fragments arranged by size:
1495 792 778 623 190
BsmI GAATG_Cn'
Cuts at: 489 489
Size: 3878
Bsp1286I G_dGCh'C
Cuts at: 253 269 575 892 1503 1596 1670 1860 1922
Size: 16 306 317 611 93 74 190 62
Cuts at: 1922 2652 253
Size: 730 1479
Fragments arranged by size:
1479 730 611 317 306 190 93 74
62 16
BspEI T'CCGG_A
Cuts at: 3098 3098
Size: 3878
BspHI T'CATG_A
Cuts at: 3054 3054
Size: 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 519 928 1142 1444 1825 519
Size: 409 214 302 381 2572
Fragments arranged by size:
2572 409 381 302 214
BsrBI CCG'CTC
Cuts at: 50 384 2170 2224 3708 3873 50
Size: 334 1786 54 1484 165 55
Fragments arranged by size:
1786 1484 334 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1790 1790
Size: 3878
BsrFI r'CCGG_y
Cuts at: 283 1876 2057 2266 283
Size: 1593 181 209 1895
Fragments arranged by size:
1895 1593 209 181
BssSI C'ACGA_G
Cuts at: 2149 2507 2149
Size: 358 3520
BstAPI GCAn_nnn'nTGC
Cuts at: 329 516 1255 329
Size: 187 739 2952
Fragments arranged by size:
2952 739 187
BstBI TT'CG_AA
Cuts at: 416 2239 416
Size: 1823 2055
BstXI CCAn_nnnn'nTGG
Cuts at: 298 351 738 298
Size: 53 387 3438
Fragments arranged by size:
3438 387 53
BstYI r'GATC_y
Cuts at: 1 473 783 903 1370 1393 1728 1974 2986
Size: 472 310 120 467 23 335 246 1012
Cuts at: 2986 3072 3084 3430 1
Size: 86 12 346 449
Fragments arranged by size:
1012 472 467 449 346 335 310 246
120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 478 478
Size: 3878
BtgI C'CryG_G
Cuts at: 107 350 107
Size: 243 3635
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1828 2009 2172 1828
Size: 181 163 3534
Fragments arranged by size:
3534 181 163
BtsI GCAGTG_nn'
Cuts at: 303 1121 303
Size: 818 3060
DraI TTT'AAA
Cuts at: 877 877
Size: 3878
DrdI GACnn_nn'nnGTC
Cuts at: 1584 2442 1584
Size: 858 3020
EaeI y'GGCC_r
Cuts at: 285 1463 1637 2028 2055 3400 285
Size: 1178 174 391 27 1345 763
Fragments arranged by size:
1345 1178 763 391 174 27
EagI C'GGCC_G
Cuts at: 1463 1463
Size: 3878
EarI CTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 375 2086 2396 2542 3688 375
Size: 1711 310 146 1146 565
Fragments arranged by size:
1711 1146 565 310 146
EcoICRI GAG'CTC
Cuts at: 890 890
Size: 3878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 763 785 818 1703 2135 2882 3150 415
Size: 348 22 33 885 432 747 268 1143
Fragments arranged by size:
1143 885 747 432 348 268 33 22
EcoNI CCTnn'n_nnAGG
Cuts at: 333 397 333
Size: 64 3814
EcoO109I rG'GnC_Cy
Cuts at: 1017 1017
Size: 3878
EcoRI G'AATT_C
Cuts at: 882 882
Size: 3878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3878
FspI TGC'GCA
Cuts at: 1659 1659
Size: 3878
HaeII r_GCGC'y
Cuts at: 280 393 1312 1560 2582 3561 280
Size: 113 919 248 1022 979 597
Fragments arranged by size:
1022 979 919 597 248 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 712 720 744 1720 1750 1752 1782 688
Size: 24 8 24 976 30 2 30 2784
Fragments arranged by size:
2784 976 30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 405 917 405
Size: 512 3366
KasI G'GCGC_C
Cuts at: 389 1308 1556 389
Size: 919 248 2711
Fragments arranged by size:
2711 919 248
KpnI G_GTAC'C
Cuts at: 898 898
Size: 3878
MluI A'CGCG_T
Cuts at: 3311 3311
Size: 3878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 614 755 2549 2733 614
Size: 141 1794 184 1759
Fragments arranged by size:
1794 1759 184 141
MscI TGG'CCA
Cuts at: 1639 1639
Size: 3878
MslI CAynn'nnrTG
Cuts at: 112 349 1867 1994 112
Size: 237 1518 127 1996
Fragments arranged by size:
1996 1518 237 127
MspA1I CmG'CkG
Cuts at: 989 1303 1663 2676 2921 989
Size: 314 360 1013 245 1946
Fragments arranged by size:
1946 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 3878
NarI GG'CG_CC
Cuts at: 390 1309 1557 390
Size: 919 248 2711
Fragments arranged by size:
2711 919 248
NcoI C'CATG_G
Cuts at: 107 350 107
Size: 243 3635
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 3878
NspI r_CATG'y
Cuts at: 609 931 1431 2338 609
Size: 322 500 907 2149
Fragments arranged by size:
2149 907 500 322
PciI A'CATG_T
Cuts at: 2334 2334
Size: 3878
PflMI CCAn_nnn'nTGG
Cuts at: 786 786
Size: 3878
PfoI T'CCnGG_A
Cuts at: 779 779
Size: 3878
PmeI GTTT'AAAC
Cuts at: 877 877
Size: 3878
PmlI CAC'GTG
Cuts at: 270 270
Size: 3878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3846
PstI C_TGCA'G
Cuts at: 248 512 925 1610 248
Size: 264 413 685 2516
Fragments arranged by size:
2516 685 413 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3878
PvuII CAG'CTG
Cuts at: 1303 1663 1303
Size: 360 3518
RsrII CG'GwC_CG
Cuts at: 2073 2073
Size: 3878
SacI G_AGCT'C
Cuts at: 892 892
Size: 3878
SalI G'TCGA_C
Cuts at: 915 915
Size: 3878
SapI GCTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
SbfI CC_TGCA'GG
Cuts at: 512 925 512
Size: 413 3465
ScaI AGT'ACT
Cuts at: 148 648 3395 148
Size: 500 2747 631
Fragments arranged by size:
2747 631 500
SfcI C'TryA_G
Cuts at: 33 244 508 543 921 1606 2599 2790 33
Size: 211 264 35 378 685 993 191 1121
Fragments arranged by size:
1121 993 685 378 264 211 191 35
SfoI GGC'GCC
Cuts at: 391 1310 1558 391
Size: 919 248 2711
Fragments arranged by size:
2711 919 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
SmaI CCC'GGG
Cuts at: 900 900
Size: 3878
SmlI C'TyrA_G
Cuts at: 424 1033 1948 2440 2702 424
Size: 609 915 492 262 1600
Fragments arranged by size:
1600 915 609 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3878
SphI G_CATG'C
Cuts at: 609 931 1431 609
Size: 322 500 3056
Fragments arranged by size:
3056 500 322
StyI C'CwwG_G
Cuts at: 107 350 464 1012 1366 107
Size: 243 114 548 354 2619
Fragments arranged by size:
2619 548 354 243 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1461 1594 2053 2262 1461
Size: 133 459 209 3077
Fragments arranged by size:
3077 459 209 133
TatI w'GTAC_w
Cuts at: 146 646 3393 146
Size: 500 2747 631
Fragments arranged by size:
2747 631 500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 3846
Tth111I GACn'n_nGTC
Cuts at: 1675 1675
Size: 3878
XbaI T'CTAG_A
Cuts at: 909 909
Size: 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 295 1373 114
Size: 181 1078 2619
Fragments arranged by size:
2619 1078 181
XmaI C'CCGG_G
Cuts at: 898 898
Size: 3878
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI