Restriction map for
pF1KB7532
(Circular) (Six-base) MAPSORT of: pF1KB7532.seq Check: 5258 from: 1 to: 3881
pF1KB7532 3881 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 249 249
Size: 3881
AccI GT'mk_AC
Cuts at: 919 919
Size: 3881
Acc65I G'GTAC_C
Cuts at: 897 897
Size: 3881
AclI AA'CG_TT
Cuts at: 3721 3721
Size: 3881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1706 2138 2885 3153 742
Size: 964 432 747 268 1470
Fragments arranged by size:
1470 964 747 432 268
AflIII A'CryG_T
Cuts at: 470 1233 2337 3314 470
Size: 763 1104 977 1037
Fragments arranged by size:
1104 1037 977 763
AgeI A'CCGG_T
Cuts at: 2269 2269
Size: 3881
AleI CACnn'nnGTG
Cuts at: 292 505 292
Size: 213 3668
AlwNI CAG_nnn'CTG
Cuts at: 241 276 402 1186 2753 241
Size: 35 126 784 1567 1369
Fragments arranged by size:
1567 1369 784 126 35
ApaI G_GGCC'C
Cuts at: 515 596 610 716 515
Size: 81 14 106 3680
Fragments arranged by size:
3680 106 81 14
ApaLI G'TGCA_C
Cuts at: 2651 2651
Size: 3881
ApoI r'AATT_y
Cuts at: 885 3234 3490 885
Size: 2349 256 1276
Fragments arranged by size:
2349 1276 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3881
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
AvaI C'yCGr_G
Cuts at: 901 901
Size: 3881
BamHI G'GATC_C
Cuts at: 906 906
Size: 3881
BanI G'GyrC_C
Cuts at: 621 897 1311 1559 1594 621
Size: 276 414 248 35 2908
Fragments arranged by size:
2908 414 276 248 35
BanII G_rGCy'C
Cuts at: 515 596 610 716 895 1925 515
Size: 81 14 106 179 1030 2471
Fragments arranged by size:
2471 1030 179 106 81 14
BbeI G_GCGC'C
Cuts at: 1315 1563 1315
Size: 248 3633
BbsI GAAGACnn'nnnn_
Cuts at: 776 776
Size: 3881
BbvCI CC'TCA_GC
Cuts at: 202 647 202
Size: 445 3436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 823 857 90
Size: 34 699 34 3114
Fragments arranged by size:
3114 699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1779 2540 3881 1779
Size: 761 1341 1779
Fragments arranged by size:
1779 1341 761
BclI T'GATC_A
Cuts at: 1401 1401
Size: 3881
BglII A'GATC_T
Cuts at: 1396 3433 1396
Size: 2037 1844
BlpI GC'TnA_GC
Cuts at: 993 993
Size: 3881
Bme1580I G_kGCm'C
Cuts at: 437 493 515 596 610 716 1506 1599 2655
Size: 56 22 81 14 106 790 93 1056
Cuts at: 2655 437
Size: 1663
Fragments arranged by size:
1663 1056 790 106 93 81 56 22
14
BmrI ACTGGGnnnn_n'
Cuts at: 711 1093 1174 1509 3653 711
Size: 382 81 335 2144 939
Fragments arranged by size:
2144 939 382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 3881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 622
Size: 3881
Bpu10I CC'TnA_GC
Cuts at: 202 647 202
Size: 445 3436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1936 2428 2726 1057
Size: 879 492 298 2212
Fragments arranged by size:
2212 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3585 3585
Size: 3881
BsaAI yAC'GTr
Cuts at: 1234 1234
Size: 3881
BsaBI GATnn'nnATC
Cuts at: 6 1395 3432 6
Size: 1389 2037 455
Fragments arranged by size:
2037 1389 455
BsaHI Gr'CG_yC
Cuts at: 170 836 1312 1560 2262 3758 170
Size: 666 476 248 702 1496 293
Fragments arranged by size:
1496 702 666 476 293 248
BsaWI w'CCGG_w
Cuts at: 132 1070 1591 2269 2543 2690 3101 132
Size: 938 521 678 274 147 411 912
Fragments arranged by size:
938 912 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 219 358 507 219
Size: 139 149 3593
Fragments arranged by size:
3593 149 139
BseYI C'CCAG_C
Cuts at: 144 374 493 699 1306 2064 2641 3137 144
Size: 230 119 206 607 758 577 496 888
Fragments arranged by size:
888 758 607 577 496 230 206 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 499 227
Size: 272 3609
BsiEI CG_ry'CG
Cuts at: 104 1423 1469 2677 104
Size: 1319 46 1208 1308
Fragments arranged by size:
1319 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 617 895 1673 1863 2655 617
Size: 278 778 190 792 1843
Fragments arranged by size:
1843 792 778 278 190
Bsp1286I G_dGCh'C
Cuts at: 437 493 515 596 610 617 716 895 1506
Size: 56 22 81 14 7 99 179 611
Cuts at: 1506 1599 1673 1863 1925 2655 437
Size: 93 74 190 62 730 1663
Fragments arranged by size:
1663 730 611 190 179 99 93 81
74 62 56 22 14 7
BspEI T'CCGG_A
Cuts at: 132 3101 132
Size: 2969 912
BspHI T'CATG_A
Cuts at: 3057 3057
Size: 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 249 931 1145 1447 1828 249
Size: 682 214 302 381 2302
Fragments arranged by size:
2302 682 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2173 2227 3711 3876 50
Size: 2123 54 1484 165 55
Fragments arranged by size:
2123 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 1793
Size: 3881
BsrFI r'CCGG_y
Cuts at: 361 1879 2060 2269 361
Size: 1518 181 209 1973
Fragments arranged by size:
1973 1518 209 181
BssSI C'ACGA_G
Cuts at: 2152 2510 2152
Size: 358 3523
BstAPI GCAn_nnn'nTGC
Cuts at: 241 1258 241
Size: 1017 2864
BstBI TT'CG_AA
Cuts at: 2242 2242
Size: 3881
BstYI r'GATC_y
Cuts at: 1 906 1373 1396 1731 1977 2989 3075 3087
Size: 905 467 23 335 246 1012 86 12
Cuts at: 3087 3433 1
Size: 346 449
Fragments arranged by size:
1012 905 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 409 640 409
Size: 231 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1831 2012 2175 1831
Size: 181 163 3537
Fragments arranged by size:
3537 181 163
BtsI GCAGTG_nn'
Cuts at: 234 341 593 1124 234
Size: 107 252 531 2991
Fragments arranged by size:
2991 531 252 107
DraI TTT'AAA
Cuts at: 880 880
Size: 3881
DrdI GACnn_nn'nnGTC
Cuts at: 1587 2445 1587
Size: 858 3023
EaeI y'GGCC_r
Cuts at: 157 386 1466 1640 2031 2058 3403 157
Size: 229 1080 174 391 27 1345 635
Fragments arranged by size:
1345 1080 635 391 229 174 27
EagI C'GGCC_G
Cuts at: 1466 1466
Size: 3881
EarI CTCTTCn'nnn_
Cuts at: 774 818 1904 2114 774
Size: 44 1086 210 2541
Fragments arranged by size:
2541 1086 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 530 564 2089 2399 2545 3691 530
Size: 34 1525 310 146 1146 720
Fragments arranged by size:
1525 1146 720 310 146 34
EcoICRI GAG'CTC
Cuts at: 893 893
Size: 3881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 742 1706 2138 2885 3153 622
Size: 120 964 432 747 268 1350
Fragments arranged by size:
1350 964 747 432 268 120
EcoO109I rG'GnC_Cy
Cuts at: 298 511 512 592 593 606 713 1020 298
Size: 213 1 80 1 13 107 307 3159
Fragments arranged by size:
3159 307 213 107 80 13 1 1
EcoRI G'AATT_C
Cuts at: 885 885
Size: 3881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3881
FspI TGC'GCA
Cuts at: 1662 1662
Size: 3881
HaeII r_GCGC'y
Cuts at: 1315 1563 2585 3564 1315
Size: 248 1022 979 1632
Fragments arranged by size:
1632 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1723 1753 1755 1785 1723
Size: 30 2 30 3819
Fragments arranged by size:
3819 30 30 2
HincII GTy'rAC
Cuts at: 527 797 920 527
Size: 270 123 3488
Fragments arranged by size:
3488 270 123
KasI G'GCGC_C
Cuts at: 1311 1559 1311
Size: 248 3633
KpnI G_GTAC'C
Cuts at: 901 901
Size: 3881
MluI A'CGCG_T
Cuts at: 3314 3314
Size: 3881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2552 2736 2552
Size: 184 3697
MscI TGG'CCA
Cuts at: 1642 1642
Size: 3881
MslI CAynn'nnrTG
Cuts at: 292 475 505 1870 1997 292
Size: 183 30 1365 127 2176
Fragments arranged by size:
2176 1365 183 127 30
MspA1I CmG'CkG
Cuts at: 452 466 600 992 1306 1666 2679 2924 452
Size: 14 134 392 314 360 1013 245 1409
Fragments arranged by size:
1409 1013 392 360 314 245 134 14
NaeI GCC'GGC
Cuts at: 363 2062 363
Size: 1699 2182
NarI GG'CG_CC
Cuts at: 1312 1560 1312
Size: 248 3633
NcoI C'CATG_G
Cuts at: 409 409
Size: 3881
NgoMIV G'CCGG_C
Cuts at: 361 2060 361
Size: 1699 2182
NspI r_CATG'y
Cuts at: 474 934 1434 2341 474
Size: 460 500 907 2014
Fragments arranged by size:
2014 907 500 460
PciI A'CATG_T
Cuts at: 470 2337 470
Size: 1867 2014
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3881
PmeI GTTT'AAAC
Cuts at: 880 880
Size: 3881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3849
PpuMI rG'GwC_Cy
Cuts at: 298 298
Size: 3881
PspOMI G'GGCC_C
Cuts at: 511 592 606 712 511
Size: 81 14 106 3680
Fragments arranged by size:
3680 106 81 14
PstI C_TGCA'G
Cuts at: 336 398 683 928 1613 336
Size: 62 285 245 685 2604
Fragments arranged by size:
2604 685 285 245 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3881
PvuII CAG'CTG
Cuts at: 452 466 1306 1666 452
Size: 14 840 360 2667
Fragments arranged by size:
2667 840 360 14
RsrII CG'GwC_CG
Cuts at: 2076 2076
Size: 3881
SacI G_AGCT'C
Cuts at: 895 895
Size: 3881
SalI G'TCGA_C
Cuts at: 918 918
Size: 3881
SapI GCTCTTCn'nnn_
Cuts at: 774 1904 2114 774
Size: 1130 210 2541
Fragments arranged by size:
2541 1130 210
SbfI CC_TGCA'GG
Cuts at: 928 928
Size: 3881
ScaI AGT'ACT
Cuts at: 3398 3398
Size: 3881
SfcI C'TryA_G
Cuts at: 33 332 394 679 924 1609 2602 2793 33
Size: 299 62 285 245 685 993 191 1121
Fragments arranged by size:
1121 993 685 299 285 245 191 62
SfoI GGC'GCC
Cuts at: 1313 1561 1313
Size: 248 3633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
SmaI CCC'GGG
Cuts at: 903 903
Size: 3881
SmlI C'TyrA_G
Cuts at: 1036 1951 2443 2705 1036
Size: 915 492 262 2212
Fragments arranged by size:
2212 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3881
SphI G_CATG'C
Cuts at: 934 1434 934
Size: 500 3381
StyI C'CwwG_G
Cuts at: 409 1015 1369 409
Size: 606 354 2921
Fragments arranged by size:
2921 606 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1464 1597 2056 2265 1464
Size: 133 459 209 3080
Fragments arranged by size:
3080 459 209 133
TatI w'GTAC_w
Cuts at: 3396 3396
Size: 3881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 282 314 3398 3430 282
Size: 32 3084 32 733
Fragments arranged by size:
3084 733 32 32
Tth111I GACn'n_nGTC
Cuts at: 1678 1678
Size: 3881
XbaI T'CTAG_A
Cuts at: 912 912
Size: 3881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 226 501 585 1376 226
Size: 275 84 791 2731
Fragments arranged by size:
2731 791 275 84
XmaI C'CCGG_G
Cuts at: 901 901
Size: 3881
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI