Restriction map for
pF1KB7536
(Circular) (Six-base) MAPSORT of: pF1KB7536.seq Check: 2429 from: 1 to: 3884
pF1KB7536 3884 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 814 814
Size: 3884
AccI GT'mk_AC
Cuts at: 922 922
Size: 3884
Acc65I G'GTAC_C
Cuts at: 498 900 498
Size: 402 3482
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2141 2888 3156 1709
Size: 432 747 268 2437
Fragments arranged by size:
2437 747 432 268
AflIII A'CryG_T
Cuts at: 1236 2340 3317 1236
Size: 1104 977 1803
Fragments arranged by size:
1803 1104 977
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 3884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 568 568
Size: 3884
AlwNI CAG_nnn'CTG
Cuts at: 513 1189 2756 513
Size: 676 1567 1641
Fragments arranged by size:
1641 1567 676
ApaI G_GGCC'C
Cuts at: 397 475 397
Size: 78 3806
ApaLI G'TGCA_C
Cuts at: 697 2654 697
Size: 1957 1927
ApoI r'AATT_y
Cuts at: 888 3237 3493 888
Size: 2349 256 1279
Fragments arranged by size:
2349 1279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 631 904 631
Size: 273 3611
BamHI G'GATC_C
Cuts at: 909 909
Size: 3884
BanI G'GyrC_C
Cuts at: 241 253 271 384 427 498 593 900 1314
Size: 12 18 113 43 71 95 307 414
Cuts at: 1314 1562 1597 241
Size: 248 35 2528
Fragments arranged by size:
2528 414 307 248 113 95 71 43
35 18 12
BanII G_rGCy'C
Cuts at: 397 475 898 1928 397
Size: 78 423 1030 2353
Fragments arranged by size:
2353 1030 423 78
BbeI G_GCGC'C
Cuts at: 245 257 275 388 431 1318 1566 245
Size: 12 18 113 43 887 248 2563
Fragments arranged by size:
2563 887 248 113 43 18 12
BbsI GAAGACnn'nnnn_
Cuts at: 201 201
Size: 3884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3850
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2543 3884 1782
Size: 761 1341 1782
Fragments arranged by size:
1782 1341 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglII A'GATC_T
Cuts at: 1399 3436 1399
Size: 2037 1847
BlpI GC'TnA_GC
Cuts at: 996 996
Size: 3884
Bme1580I G_kGCm'C
Cuts at: 397 475 535 673 701 1509 1602 2658 397
Size: 78 60 138 28 808 93 1056 1623
Fragments arranged by size:
1623 1056 808 138 93 78 60 28
BmgBI CAC'GTC
Cuts at: 666 666
Size: 3884
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2431 2729 1060
Size: 879 492 298 2215
Fragments arranged by size:
2215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 643 3588 643
Size: 2945 939
BsaAI yAC'GTr
Cuts at: 375 1237 375
Size: 862 3022
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 242 254 272 385 428 454 811 826 1315
Size: 12 18 113 43 26 357 15 489
Cuts at: 1315 1563 2265 3761 242
Size: 248 702 1496 365
Fragments arranged by size:
1496 702 489 365 357 248 113 43
26 18 15 12
BsaWI w'CCGG_w
Cuts at: 831 1073 1594 2272 2546 2693 3104 831
Size: 242 521 678 274 147 411 1611
Fragments arranged by size:
1611 678 521 411 274 242 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 712 742 640
Size: 30 42 30 3782
Fragments arranged by size:
3782 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 724 793 249
Size: 475 69 3340
Fragments arranged by size:
3340 475 69
BseYI C'CCAG_C
Cuts at: 423 431 1309 2067 2644 3140 423
Size: 8 878 758 577 496 1167
Fragments arranged by size:
1167 878 758 577 496 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 355 397 556 205
Size: 150 42 159 3533
Fragments arranged by size:
3533 159 150 42
BsiEI CG_ry'CG
Cuts at: 104 154 320 1426 1472 2680 104
Size: 50 166 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 166 50 46
BsiHKAI G_wGCw'C
Cuts at: 442 701 898 1676 1866 2658 442
Size: 259 197 778 190 792 1668
Fragments arranged by size:
1668 792 778 259 197 190
Bsp1286I G_dGCh'C
Cuts at: 397 442 475 535 673 701 898 1509 1602
Size: 45 33 60 138 28 197 611 93
Cuts at: 1602 1676 1866 1928 2658 397
Size: 74 190 62 730 1623
Fragments arranged by size:
1623 730 611 197 190 138 93 74
62 60 45 33 28
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 934 1148 1450 1831 934
Size: 214 302 381 2987
Fragments arranged by size:
2987 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2176 2230 3714 3879 50
Size: 2126 54 1484 165 55
Fragments arranged by size:
2126 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1796 1796
Size: 3884
BsrFI r'CCGG_y
Cuts at: 177 1882 2063 2272 177
Size: 1705 181 209 1789
Fragments arranged by size:
1789 1705 209 181
BssSI C'ACGA_G
Cuts at: 2155 2513 2155
Size: 358 3526
BstAPI GCAn_nnn'nTGC
Cuts at: 510 1261 510
Size: 751 3133
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstYI r'GATC_y
Cuts at: 1 909 1376 1399 1734 1980 2992 3078 3090
Size: 908 467 23 335 246 1012 86 12
Cuts at: 3090 3436 1
Size: 346 449
Fragments arranged by size:
1012 908 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 290 320 389 290
Size: 30 69 3785
Fragments arranged by size:
3785 69 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1834 2015 2178 1834
Size: 181 163 3540
Fragments arranged by size:
3540 181 163
BtsI GCAGTG_nn'
Cuts at: 442 1127 442
Size: 685 3199
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 816 1590 2448 816
Size: 774 858 2252
Fragments arranged by size:
2252 858 774
EaeI y'GGCC_r
Cuts at: 151 317 1469 1643 2034 2061 3406 151
Size: 166 1152 174 391 27 1345 629
Fragments arranged by size:
1345 1152 629 391 174 166 27
EagI C'GGCC_G
Cuts at: 151 317 1469 151
Size: 166 1152 2566
Fragments arranged by size:
2566 1152 166
EarI CTCTTCn'nnn_
Cuts at: 646 832 839 1907 2117 646
Size: 186 7 1068 210 2413
Fragments arranged by size:
2413 1068 210 186 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 597 2092 2402 2548 3694 597
Size: 1495 310 146 1146 787
Fragments arranged by size:
1495 1146 787 310 146
EcoICRI GAG'CTC
Cuts at: 896 896
Size: 3884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2141 2888 3156 1709
Size: 432 747 268 2437
Fragments arranged by size:
2437 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 643 706 643
Size: 63 3821
EcoO109I rG'GnC_Cy
Cuts at: 471 472 1023 471
Size: 1 551 3332
Fragments arranged by size:
3332 551 1
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 245 257 275 388 431 1318 1566 2588 3567
Size: 12 18 113 43 887 248 1022 979
Cuts at: 3567 245
Size: 562
Fragments arranged by size:
1022 979 887 562 248 113 43 18
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 853 885 1726 1756 1758 1788 853
Size: 32 841 30 2 30 2949
Fragments arranged by size:
2949 841 32 30 30 2
HincII GTy'rAC
Cuts at: 923 923
Size: 3884
KasI G'GCGC_C
Cuts at: 241 253 271 384 427 1314 1562 241
Size: 12 18 113 43 887 248 2563
Fragments arranged by size:
2563 887 248 113 43 18 12
KpnI G_GTAC'C
Cuts at: 502 904 502
Size: 402 3482
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 764 2555 2739 764
Size: 1791 184 1909
Fragments arranged by size:
1909 1791 184
MscI TGG'CCA
Cuts at: 1645 1645
Size: 3884
MslI CAynn'nnrTG
Cuts at: 526 1873 2000 526
Size: 1347 127 2410
Fragments arranged by size:
2410 1347 127
MspA1I CmG'CkG
Cuts at: 248 322 329 369 423 570 684 995 1309
Size: 74 7 40 54 147 114 311 314
Cuts at: 1309 1669 2682 2927 248
Size: 360 1013 245 1205
Fragments arranged by size:
1205 1013 360 314 311 245 147 114
74 54 40 7
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 242 254 272 385 428 1315 1563 242
Size: 12 18 113 43 887 248 2563
Fragments arranged by size:
2563 887 248 113 43 18 12
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 123 937 1437 2344 123
Size: 814 500 907 1663
Fragments arranged by size:
1663 907 814 500
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PfoI T'CCnGG_A
Cuts at: 644 644
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PmlI CAC'GTG
Cuts at: 375 375
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3852
PspOMI G'GGCC_C
Cuts at: 393 471 393
Size: 78 3806
PstI C_TGCA'G
Cuts at: 557 931 1616 557
Size: 374 685 2825
Fragments arranged by size:
2825 685 374
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 369 570 684 1309 1669 369
Size: 201 114 625 360 2584
Fragments arranged by size:
2584 625 360 201 114
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 898 898
Size: 3884
SacII CC_GC'GG
Cuts at: 323 323
Size: 3884
SalI G'TCGA_C
Cuts at: 921 921
Size: 3884
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 931 931
Size: 3884
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 553 927 1612 2605 2796 33
Size: 520 374 685 993 191 1121
Fragments arranged by size:
1121 993 685 520 374 191
SfoI GGC'GCC
Cuts at: 243 255 273 386 429 1316 1564 243
Size: 12 18 113 43 887 248 2563
Fragments arranged by size:
2563 887 248 113 43 18 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 631 1039 1954 2446 2708 631
Size: 408 915 492 262 1807
Fragments arranged by size:
1807 915 492 408 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
StyI C'CwwG_G
Cuts at: 1018 1372 1018
Size: 354 3530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 1600 2059 2268 1467
Size: 133 459 209 3083
Fragments arranged by size:
3083 459 209 133
TatI w'GTAC_w
Cuts at: 3399 3399
Size: 3884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 3852
Tth111I GACn'n_nGTC
Cuts at: 813 1681 813
Size: 868 3016
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1379 1379
Size: 3884
XhoI C'TCGA_G
Cuts at: 631 631
Size: 3884
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
XmnI GAAnn'nnTTC
Cuts at: 640 640
Size: 3884
ZraI GAC'GTC
Cuts at: 812 812
Size: 3884
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PpuMI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI