Restriction map for
pF1KB7537
(Circular) (Six-base) MAPSORT of: pF1KB7537.seq Check: 2915 from: 1 to: 3632
pF1KB7537 3632 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 284 284
Size: 3632
AccI GT'mk_AC
Cuts at: 670 670
Size: 3632
Acc65I G'GTAC_C
Cuts at: 648 648
Size: 3632
AclI AA'CG_TT
Cuts at: 3472 3472
Size: 3632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1457 1889 2636 2904 178
Size: 1279 432 747 268 906
Fragments arranged by size:
1279 906 747 432 268
AflII C'TTAA_G
Cuts at: 214 214
Size: 3632
AflIII A'CryG_T
Cuts at: 984 2088 3065 984
Size: 1104 977 1551
Fragments arranged by size:
1551 1104 977
AgeI A'CCGG_T
Cuts at: 2020 2020
Size: 3632
AlwNI CAG_nnn'CTG
Cuts at: 204 315 383 937 2504 204
Size: 111 68 554 1567 1332
Fragments arranged by size:
1567 1332 554 111 68
ApaLI G'TGCA_C
Cuts at: 2402 2402
Size: 3632
ApoI r'AATT_y
Cuts at: 636 2985 3241 636
Size: 2349 256 1027
Fragments arranged by size:
2349 1027 256
AscI GG'CGCG_CC
Cuts at: 391 391
Size: 3632
AseI AT'TA_AT
Cuts at: 20 20
Size: 3632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
AvaI C'yCGr_G
Cuts at: 652 652
Size: 3632
BamHI G'GATC_C
Cuts at: 657 657
Size: 3632
BanI G'GyrC_C
Cuts at: 576 648 1062 1310 1345 576
Size: 72 414 248 35 2863
Fragments arranged by size:
2863 414 248 72 35
BanII G_rGCy'C
Cuts at: 224 434 563 646 1676 224
Size: 210 129 83 1030 2180
Fragments arranged by size:
2180 1030 210 129 83
BbeI G_GCGC'C
Cuts at: 1066 1314 1066
Size: 248 3384
BciVI GTATCCnnnnn_n'
Cuts at: 1530 2291 3632 1530
Size: 761 1341 1530
Fragments arranged by size:
1530 1341 761
BclI T'GATC_A
Cuts at: 1152 1152
Size: 3632
BglI GCCn_nnn'nGGC
Cuts at: 388 388
Size: 3632
BglII A'GATC_T
Cuts at: 1147 3184 1147
Size: 2037 1595
BlpI GC'TnA_GC
Cuts at: 744 744
Size: 3632
Bme1580I G_kGCm'C
Cuts at: 581 1257 1350 2406 581
Size: 676 93 1056 1807
Fragments arranged by size:
1807 1056 676 93
BmrI ACTGGGnnnn_n'
Cuts at: 844 925 1260 3404 844
Size: 81 335 2144 1072
Fragments arranged by size:
2144 1072 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 448 475 190
Size: 258 27 3347
Fragments arranged by size:
3347 258 27
Bpu10I CC'TnA_GC
Cuts at: 205 205
Size: 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1687 2179 2477 808
Size: 879 492 298 1963
Fragments arranged by size:
1963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3336 3336
Size: 3632
BsaAI yAC'GTr
Cuts at: 985 985
Size: 3632
BsaBI GATnn'nnATC
Cuts at: 6 1146 3183 6
Size: 1140 2037 455
Fragments arranged by size:
2037 1140 455
BsaHI Gr'CG_yC
Cuts at: 281 1063 1311 2013 3509 281
Size: 782 248 702 1496 404
Fragments arranged by size:
1496 782 702 404 248
BsaWI w'CCGG_w
Cuts at: 573 821 1342 2020 2294 2441 2852 573
Size: 248 521 678 274 147 411 1353
Fragments arranged by size:
1353 678 521 411 274 248 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 448
Size: 30 3602
BseYI C'CCAG_C
Cuts at: 237 505 537 1057 1815 2392 2888 237
Size: 268 32 520 758 577 496 981
Fragments arranged by size:
981 758 577 520 496 268 32
BsiEI CG_ry'CG
Cuts at: 104 622 1174 1220 2428 104
Size: 518 552 46 1208 1308
Fragments arranged by size:
1308 1208 552 518 46
BsiHKAI G_wGCw'C
Cuts at: 461 646 1424 1614 2406 461
Size: 185 778 190 792 1687
Fragments arranged by size:
1687 792 778 190 185
Bsp1286I G_dGCh'C
Cuts at: 224 434 461 563 581 646 1257 1350 1424
Size: 210 27 102 18 65 611 93 74
Cuts at: 1424 1614 1676 2406 224
Size: 190 62 730 1450
Fragments arranged by size:
1450 730 611 210 190 102 93 74
65 62 27 18
BspEI T'CCGG_A
Cuts at: 2852 2852
Size: 3632
BspHI T'CATG_A
Cuts at: 2808 2808
Size: 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 682 896 1198 1579 682
Size: 214 302 381 2735
Fragments arranged by size:
2735 381 302 214
BsrBI CCG'CTC
Cuts at: 50 475 571 1924 1978 3462 3627 50
Size: 425 96 1353 54 1484 165 55
Fragments arranged by size:
1484 1353 425 165 96 55 54
BsrDI GCAATG_nn'
Cuts at: 1544 1544
Size: 3632
BsrFI r'CCGG_y
Cuts at: 179 1630 1811 2020 179
Size: 1451 181 209 1791
Fragments arranged by size:
1791 1451 209 181
BssHII G'CGCG_C
Cuts at: 183 185 187 391 443 445 501 183
Size: 2 2 204 52 2 56 3314
Fragments arranged by size:
3314 204 56 52 2 2 2
BssSI C'ACGA_G
Cuts at: 1903 2261 1903
Size: 358 3274
BstAPI GCAn_nnn'nTGC
Cuts at: 315 1009 315
Size: 694 2938
BstBI TT'CG_AA
Cuts at: 1993 1993
Size: 3632
BstYI r'GATC_y
Cuts at: 1 657 1124 1147 1482 1728 2740 2826 2838
Size: 656 467 23 335 246 1012 86 12
Cuts at: 2838 3184 1
Size: 346 449
Fragments arranged by size:
1012 656 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 335 622 107
Size: 228 287 3117
Fragments arranged by size:
3117 287 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1582 1763 1926 1582
Size: 181 163 3288
Fragments arranged by size:
3288 181 163
BtsI GCAGTG_nn'
Cuts at: 875 875
Size: 3632
DraI TTT'AAA
Cuts at: 631 631
Size: 3632
DrdI GACnn_nn'nnGTC
Cuts at: 1338 2196 1338
Size: 858 2774
EaeI y'GGCC_r
Cuts at: 476 619 1217 1391 1782 1809 3154 476
Size: 143 598 174 391 27 1345 954
Fragments arranged by size:
1345 954 598 391 174 143 27
EagI C'GGCC_G
Cuts at: 619 1217 619
Size: 598 3034
EarI CTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 483 1840 2150 2296 3442 154
Size: 329 1357 310 146 1146 344
Fragments arranged by size:
1357 1146 344 329 310 146
EcoICRI GAG'CTC
Cuts at: 644 644
Size: 3632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 190 448 475 1457 1889 2636 2904 178
Size: 12 258 27 982 432 747 268 906
Fragments arranged by size:
982 906 747 432 268 258 27 12
EcoO109I rG'GnC_Cy
Cuts at: 771 771
Size: 3632
EcoRI G'AATT_C
Cuts at: 636 636
Size: 3632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1651 1651
Size: 3632
FspI TGC'GCA
Cuts at: 148 1413 148
Size: 1265 2367
HaeII r_GCGC'y
Cuts at: 180 346 383 485 544 1066 1314 2336 3315
Size: 166 37 102 59 522 248 1022 979
Cuts at: 3315 180
Size: 497
Fragments arranged by size:
1022 979 522 497 248 166 102 59
37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 550 582 1474 1504 1506 1536 550
Size: 32 892 30 2 30 2646
Fragments arranged by size:
2646 892 32 30 30 2
HincII GTy'rAC
Cuts at: 671 671
Size: 3632
KasI G'GCGC_C
Cuts at: 1062 1310 1062
Size: 248 3384
KpnI G_GTAC'C
Cuts at: 652 652
Size: 3632
MluI A'CGCG_T
Cuts at: 3065 3065
Size: 3632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 278 2303 2487 278
Size: 2025 184 1423
Fragments arranged by size:
2025 1423 184
MscI TGG'CCA
Cuts at: 1393 1393
Size: 3632
MslI CAynn'nnrTG
Cuts at: 1621 1748 1621
Size: 127 3505
MspA1I CmG'CkG
Cuts at: 165 234 743 1057 1417 2430 2675 165
Size: 69 509 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 509 360 314 245 69
NaeI GCC'GGC
Cuts at: 181 1813 181
Size: 1632 2000
NarI GG'CG_CC
Cuts at: 1063 1311 1063
Size: 248 3384
NcoI C'CATG_G
Cuts at: 107 107
Size: 3632
NgoMIV G'CCGG_C
Cuts at: 179 1811 179
Size: 1632 2000
NspI r_CATG'y
Cuts at: 685 1185 2092 685
Size: 500 907 2225
Fragments arranged by size:
2225 907 500
PciI A'CATG_T
Cuts at: 2088 2088
Size: 3632
PfoI T'CCnGG_A
Cuts at: 284 425 284
Size: 141 3491
PmeI GTTT'AAAC
Cuts at: 631 631
Size: 3632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 3600
PstI C_TGCA'G
Cuts at: 311 679 1364 311
Size: 368 685 2579
Fragments arranged by size:
2579 685 368
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3632
PvuII CAG'CTG
Cuts at: 1057 1417 1057
Size: 360 3272
RsrII CG'GwC_CG
Cuts at: 1827 1827
Size: 3632
SacI G_AGCT'C
Cuts at: 646 646
Size: 3632
SalI G'TCGA_C
Cuts at: 669 669
Size: 3632
SapI GCTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
SbfI CC_TGCA'GG
Cuts at: 311 679 311
Size: 368 3264
ScaI AGT'ACT
Cuts at: 3149 3149
Size: 3632
SfcI C'TryA_G
Cuts at: 33 307 375 675 1360 2353 2544 33
Size: 274 68 300 685 993 191 1121
Fragments arranged by size:
1121 993 685 300 274 191 68
SfoI GGC'GCC
Cuts at: 1064 1312 1064
Size: 248 3384
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
SgrAI Cr'CCGG_yG
Cuts at: 179 179
Size: 3632
SmaI CCC'GGG
Cuts at: 654 654
Size: 3632
SmlI C'TyrA_G
Cuts at: 214 787 1702 2194 2456 214
Size: 573 915 492 262 1390
Fragments arranged by size:
1390 915 573 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3632
SphI G_CATG'C
Cuts at: 685 1185 685
Size: 500 3132
StyI C'CwwG_G
Cuts at: 107 766 1120 107
Size: 659 354 2619
Fragments arranged by size:
2619 659 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1215 1348 1807 2016 1215
Size: 133 459 209 2831
Fragments arranged by size:
2831 459 209 133
TatI w'GTAC_w
Cuts at: 3147 3147
Size: 3632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 3149 3181 448
Size: 32 2669 32 899
Fragments arranged by size:
2669 899 32 32
Tth111I GACn'n_nGTC
Cuts at: 1429 1429
Size: 3632
XbaI T'CTAG_A
Cuts at: 663 663
Size: 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 1127 486
Size: 641 2991
XmaI C'CCGG_G
Cuts at: 652 652
Size: 3632
ZraI GAC'GTC
Cuts at: 282 282
Size: 3632
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI ApaI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI XmnI