Restriction map for
pF1KB7538
(Circular) (Six-base) MAPSORT of: pF1KB7538.seq Check: 6075 from: 1 to: 3887
pF1KB7538 3887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2144 2891 3159 1712
Size: 432 747 268 2440
Fragments arranged by size:
2440 747 432 268
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 1192 2759 1192
Size: 1567 2320
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 177 891 3240 3496 177
Size: 714 2349 256 568
Fragments arranged by size:
2349 714 568 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 288 907 288
Size: 619 3268
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 520 566 903 1317 1565 1600 520
Size: 46 337 414 248 35 2807
Fragments arranged by size:
2807 414 337 248 46 35
BanII G_rGCy'C
Cuts at: 252 271 584 901 1931 252
Size: 19 313 317 1030 2208
Fragments arranged by size:
2208 1030 317 313 19
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BbsI GAAGACnn'nnnn_
Cuts at: 130 130
Size: 3887
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 523 1512 1605 2661 523
Size: 989 93 1056 1749
Fragments arranged by size:
1749 1056 989 93
BmrI ACTGGGnnnn_n'
Cuts at: 858 1099 1180 1515 3659 858
Size: 241 81 335 2144 1086
Fragments arranged by size:
2144 1086 335 241 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 607 228
Size: 379 3508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3591 3591
Size: 3887
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 1318 1566 2268 3764 1318
Size: 248 702 1496 1441
Fragments arranged by size:
1496 1441 702 248
BsaWI w'CCGG_w
Cuts at: 1076 1597 2275 2549 2696 3107 1076
Size: 521 678 274 147 411 1856
Fragments arranged by size:
1856 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 498 610 498
Size: 112 3775
BseYI C'CCAG_C
Cuts at: 584 1312 2070 2647 3143 584
Size: 728 758 577 496 1328
Fragments arranged by size:
1328 758 728 577 496
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 252 901 1679 1869 2661 252
Size: 649 778 190 792 1478
Fragments arranged by size:
1478 792 778 649 190
BsmBI CGTCTCn'nnnn_
Cuts at: 362 362
Size: 3887
Bsp1286I G_dGCh'C
Cuts at: 252 271 523 584 901 1512 1605 1679 1869
Size: 19 252 61 317 611 93 74 190
Cuts at: 1869 1931 2661 252
Size: 62 730 1478
Fragments arranged by size:
1478 730 611 317 252 190 93 74
62 61 19
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 728 878 937 1151 1453 1834 728
Size: 150 59 214 302 381 2781
Fragments arranged by size:
2781 381 302 214 150 59
BsrBI CCG'CTC
Cuts at: 50 562 2179 2233 3717 3882 50
Size: 512 1617 54 1484 165 55
Fragments arranged by size:
1617 1484 512 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 1885 2066 2275 1885
Size: 181 209 3497
Fragments arranged by size:
3497 209 181
BssSI C'ACGA_G
Cuts at: 761 2158 2516 761
Size: 1397 358 2132
Fragments arranged by size:
2132 1397 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstXI CCAn_nnnn'nTGG
Cuts at: 344 592 694 848 344
Size: 248 102 154 3383
Fragments arranged by size:
3383 248 154 102
BstYI r'GATC_y
Cuts at: 1 766 912 1379 1402 1737 1983 2995 3081
Size: 765 146 467 23 335 246 1012 86
Cuts at: 3081 3093 3439 1
Size: 12 346 449
Fragments arranged by size:
1012 765 467 449 346 335 246 146
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 650 650
Size: 3887
BtgI C'CryG_G
Cuts at: 107 361 434 107
Size: 254 73 3560
Fragments arranged by size:
3560 254 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
ClaI AT'CG_AT
Cuts at: 864 864
Size: 3887
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 684 1472 1646 2037 2064 3409 684
Size: 788 174 391 27 1345 1162
Fragments arranged by size:
1345 1162 788 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 718 1910 2120 718
Size: 1192 210 2485
Fragments arranged by size:
2485 1192 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 250 899 250
Size: 649 3238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 607 1712 2144 2891 3159 228
Size: 379 1105 432 747 268 956
Fragments arranged by size:
1105 956 747 432 379 268
EcoO109I rG'GnC_Cy
Cuts at: 473 1026 473
Size: 553 3334
EcoRI G'AATT_C
Cuts at: 177 891 177
Size: 714 3173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 1321 1569 2591 3570 1321
Size: 248 1022 979 1638
Fragments arranged by size:
1638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 1729 1759 1761 1791 316
Size: 32 1381 30 2 30 2412
Fragments arranged by size:
2412 1381 32 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 686 1648 686
Size: 962 2925
MslI CAynn'nnrTG
Cuts at: 439 1876 2003 439
Size: 1437 127 2323
Fragments arranged by size:
2323 1437 127
MspA1I CmG'CkG
Cuts at: 998 1312 1672 2685 2930 998
Size: 314 360 1013 245 1955
Fragments arranged by size:
1955 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NcoI C'CATG_G
Cuts at: 107 361 434 107
Size: 254 73 3560
Fragments arranged by size:
3560 254 73
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NspI r_CATG'y
Cuts at: 333 424 940 1440 2347 333
Size: 91 516 500 907 1873
Fragments arranged by size:
1873 907 516 500 91
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PspXI vC'TCGA_Gb
Cuts at: 288 288
Size: 3887
PstI C_TGCA'G
Cuts at: 875 934 1619 875
Size: 59 685 3143
Fragments arranged by size:
3143 685 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 1312 1672 1312
Size: 360 3527
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 252 901 252
Size: 649 3238
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 718 1910 2120 718
Size: 1192 210 2485
Fragments arranged by size:
2485 1192 210
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SexAI A'CCwGG_T
Cuts at: 569 569
Size: 3887
SfcI C'TryA_G
Cuts at: 33 871 930 1615 2608 2799 33
Size: 838 59 685 993 191 1121
Fragments arranged by size:
1121 993 838 685 191 59
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 288 1042 1957 2449 2711 288
Size: 754 915 492 262 1464
Fragments arranged by size:
1464 915 754 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 107 207 361 434 1021 1375 107
Size: 100 154 73 587 354 2619
Fragments arranged by size:
2619 587 354 154 100 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 872 1470 1603 2062 2271 872
Size: 598 133 459 209 2488
Fragments arranged by size:
2488 598 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 845 1382 845
Size: 537 3350
XhoI C'TCGA_G
Cuts at: 288 288
Size: 3887
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
XmnI GAAnn'nnTTC
Cuts at: 448 448
Size: 3887
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI