Restriction map for
pF1KB7539
(Circular) (Six-base) MAPSORT of: pF1KB7539.seq Check: 3850 from: 1 to: 3644
pF1KB7539 3644 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 682 682
Size: 3644
Acc65I G'GTAC_C
Cuts at: 660 660
Size: 3644
AclI AA'CG_TT
Cuts at: 3484 3484
Size: 3644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1469 1901 2648 2916 298
Size: 1171 432 747 268 1026
Fragments arranged by size:
1171 1026 747 432 268
AflIII A'CryG_T
Cuts at: 996 2100 3077 996
Size: 1104 977 1563
Fragments arranged by size:
1563 1104 977
AgeI A'CCGG_T
Cuts at: 2032 2032
Size: 3644
AleI CACnn'nnGTG
Cuts at: 508 508
Size: 3644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 633 633
Size: 3644
AlwNI CAG_nnn'CTG
Cuts at: 949 2516 949
Size: 1567 2077
ApaI G_GGCC'C
Cuts at: 156 156
Size: 3644
ApaLI G'TGCA_C
Cuts at: 2414 2414
Size: 3644
ApoI r'AATT_y
Cuts at: 478 648 2997 3253 478
Size: 170 2349 256 869
Fragments arranged by size:
2349 869 256 170
AseI AT'TA_AT
Cuts at: 20 20
Size: 3644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
AvaI C'yCGr_G
Cuts at: 155 664 155
Size: 509 3135
BamHI G'GATC_C
Cuts at: 669 669
Size: 3644
BanI G'GyrC_C
Cuts at: 602 660 1074 1322 1357 602
Size: 58 414 248 35 2889
Fragments arranged by size:
2889 414 248 58 35
BanII G_rGCy'C
Cuts at: 156 377 658 1688 156
Size: 221 281 1030 2112
Fragments arranged by size:
2112 1030 281 221
BbeI G_GCGC'C
Cuts at: 1078 1326 1078
Size: 248 3396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 377 411 377
Size: 34 3610
BciVI GTATCCnnnnn_n'
Cuts at: 1542 2303 3644 1542
Size: 761 1341 1542
Fragments arranged by size:
1542 1341 761
BclI T'GATC_A
Cuts at: 1164 1164
Size: 3644
BglII A'GATC_T
Cuts at: 1159 3196 1159
Size: 2037 1607
BlpI GC'TnA_GC
Cuts at: 756 756
Size: 3644
Bme1580I G_kGCm'C
Cuts at: 156 605 1269 1362 2418 156
Size: 449 664 93 1056 1382
Fragments arranged by size:
1382 1056 664 449 93
BmrI ACTGGGnnnn_n'
Cuts at: 159 856 937 1272 3416 159
Size: 697 81 335 2144 387
Fragments arranged by size:
2144 697 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1699 2191 2489 820
Size: 879 492 298 1975
Fragments arranged by size:
1975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3348 3348
Size: 3644
BsaAI yAC'GTr
Cuts at: 997 997
Size: 3644
BsaBI GATnn'nnATC
Cuts at: 6 308 1158 3195 6
Size: 302 850 2037 455
Fragments arranged by size:
2037 850 455 302
BsaHI Gr'CG_yC
Cuts at: 1075 1323 2025 3521 1075
Size: 248 702 1496 1198
Fragments arranged by size:
1496 1198 702 248
BsaWI w'CCGG_w
Cuts at: 833 1354 2032 2306 2453 2864 833
Size: 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1069 1827 2404 2900 1069
Size: 758 577 496 1813
Fragments arranged by size:
1813 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 486
Size: 3644
BsiEI CG_ry'CG
Cuts at: 104 1186 1232 2440 104
Size: 1082 46 1208 1308
Fragments arranged by size:
1308 1208 1082 46
BsiHKAI G_wGCw'C
Cuts at: 658 1436 1626 2418 658
Size: 778 190 792 1884
Fragments arranged by size:
1884 792 778 190
BsmI GAATG_Cn'
Cuts at: 622 622
Size: 3644
BsmBI CGTCTCn'nnnn_
Cuts at: 257 257
Size: 3644
Bsp1286I G_dGCh'C
Cuts at: 156 377 605 658 1269 1362 1436 1626 1688
Size: 221 228 53 611 93 74 190 62
Cuts at: 1688 2418 156
Size: 730 1382
Fragments arranged by size:
1382 730 611 228 221 190 93 74
62 53
BspEI T'CCGG_A
Cuts at: 2864 2864
Size: 3644
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 694 908 1210 1591 694
Size: 214 302 381 2747
Fragments arranged by size:
2747 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1936 1990 3474 3639 50
Size: 1886 54 1484 165 55
Fragments arranged by size:
1886 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 123 353 400 1556 123
Size: 230 47 1156 2211
Fragments arranged by size:
2211 1156 230 47
BsrFI r'CCGG_y
Cuts at: 1642 1823 2032 1642
Size: 181 209 3254
Fragments arranged by size:
3254 209 181
BssSI C'ACGA_G
Cuts at: 431 1915 2273 431
Size: 1484 358 1802
Fragments arranged by size:
1802 1484 358
BstAPI GCAn_nnn'nTGC
Cuts at: 122 1021 122
Size: 899 2745
BstBI TT'CG_AA
Cuts at: 2005 2005
Size: 3644
BstYI r'GATC_y
Cuts at: 1 669 1136 1159 1494 1740 2752 2838 2850
Size: 668 467 23 335 246 1012 86 12
Cuts at: 2850 3196 1
Size: 346 449
Fragments arranged by size:
1012 668 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 180 201 180
Size: 21 3623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1594 1775 1938 1594
Size: 181 163 3300
Fragments arranged by size:
3300 181 163
BtsI GCAGTG_nn'
Cuts at: 887 887
Size: 3644
DraI TTT'AAA
Cuts at: 643 643
Size: 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1350 2208 1350
Size: 858 2786
EaeI y'GGCC_r
Cuts at: 144 348 1229 1403 1794 1821 3166 144
Size: 204 881 174 391 27 1345 622
Fragments arranged by size:
1345 881 622 391 204 174 27
EagI C'GGCC_G
Cuts at: 1229 1229
Size: 3644
EarI CTCTTCn'nnn_
Cuts at: 260 1667 1877 260
Size: 1407 210 2027
Fragments arranged by size:
2027 1407 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1852 2162 2308 3454 1852
Size: 310 146 1146 2042
Fragments arranged by size:
2042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 656 656
Size: 3644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1469 1901 2648 2916 298
Size: 1171 432 747 268 1026
Fragments arranged by size:
1171 1026 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 783 783
Size: 3644
EcoRI G'AATT_C
Cuts at: 478 648 478
Size: 170 3474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3644
FspI TGC'GCA
Cuts at: 1425 1425
Size: 3644
HaeII r_GCGC'y
Cuts at: 1078 1326 2348 3327 1078
Size: 248 1022 979 1395
Fragments arranged by size:
1395 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1486 1516 1518 1548 1486
Size: 30 2 30 3582
Fragments arranged by size:
3582 30 30 2
HincII GTy'rAC
Cuts at: 576 683 576
Size: 107 3537
KasI G'GCGC_C
Cuts at: 1074 1322 1074
Size: 248 3396
KpnI G_GTAC'C
Cuts at: 664 664
Size: 3644
MfeI C'AATT_G
Cuts at: 623 623
Size: 3644
MluI A'CGCG_T
Cuts at: 3077 3077
Size: 3644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2499 2315
Size: 184 3460
MscI TGG'CCA
Cuts at: 146 350 1405 146
Size: 204 1055 2385
Fragments arranged by size:
2385 1055 204
MslI CAynn'nnrTG
Cuts at: 449 508 615 1633 1760 449
Size: 59 107 1018 127 2333
Fragments arranged by size:
2333 1018 127 107 59
MspA1I CmG'CkG
Cuts at: 587 755 1069 1429 2442 2687 587
Size: 168 314 360 1013 245 1544
Fragments arranged by size:
1544 1013 360 314 245 168
NaeI GCC'GGC
Cuts at: 1825 1825
Size: 3644
NarI GG'CG_CC
Cuts at: 1075 1323 1075
Size: 248 3396
NgoMIV G'CCGG_C
Cuts at: 1823 1823
Size: 3644
NspI r_CATG'y
Cuts at: 697 1197 2104 697
Size: 500 907 2237
Fragments arranged by size:
2237 907 500
PciI A'CATG_T
Cuts at: 2100 2100
Size: 3644
PmeI GTTT'AAAC
Cuts at: 643 643
Size: 3644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 3612
PspOMI G'GGCC_C
Cuts at: 152 152
Size: 3644
PstI C_TGCA'G
Cuts at: 559 691 1376 559
Size: 132 685 2827
Fragments arranged by size:
2827 685 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3644
PvuII CAG'CTG
Cuts at: 1069 1429 1069
Size: 360 3284
RsrII CG'GwC_CG
Cuts at: 1839 1839
Size: 3644
SacI G_AGCT'C
Cuts at: 658 658
Size: 3644
SalI G'TCGA_C
Cuts at: 681 681
Size: 3644
SapI GCTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
SbfI CC_TGCA'GG
Cuts at: 691 691
Size: 3644
ScaI AGT'ACT
Cuts at: 3161 3161
Size: 3644
SfcI C'TryA_G
Cuts at: 33 519 555 632 687 1372 2365 2556 33
Size: 486 36 77 55 685 993 191 1121
Fragments arranged by size:
1121 993 685 486 191 77 55 36
SfoI GGC'GCC
Cuts at: 1076 1324 1076
Size: 248 3396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
SmaI CCC'GGG
Cuts at: 157 666 157
Size: 509 3135
SmlI C'TyrA_G
Cuts at: 799 1714 2206 2468 799
Size: 915 492 262 1975
Fragments arranged by size:
1975 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3644
SphI G_CATG'C
Cuts at: 697 1197 697
Size: 500 3144
StyI C'CwwG_G
Cuts at: 299 359 778 1132 299
Size: 60 419 354 2811
Fragments arranged by size:
2811 419 354 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1227 1360 1819 2028 1227
Size: 133 459 209 2843
Fragments arranged by size:
2843 459 209 133
TatI w'GTAC_w
Cuts at: 3159 3159
Size: 3644
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3612
Tth111I GACn'n_nGTC
Cuts at: 1441 1441
Size: 3644
XbaI T'CTAG_A
Cuts at: 675 675
Size: 3644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1139 1139
Size: 3644
XmaI C'CCGG_G
Cuts at: 155 664 155
Size: 509 3135
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI