Restriction map for
pF1KB7540
(Circular) (Six-base) MAPSORT of: pF1KB7540.seq Check: 9953 from: 1 to: 3887
pF1KB7540 3887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2144 2891 3159 1712
Size: 432 747 268 2440
Fragments arranged by size:
2440 747 432 268
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 453 654 690 780 1192 2759 453
Size: 201 36 90 412 1567 1581
Fragments arranged by size:
1581 1567 412 201 90 36
ApaI G_GGCC'C
Cuts at: 381 683 842 381
Size: 302 159 3426
Fragments arranged by size:
3426 302 159
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 231 249 269 585 842 907 231
Size: 18 20 316 257 65 3211
Fragments arranged by size:
3211 316 257 65 20 18
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 265 666 903 1317 1565 1600 265
Size: 401 237 414 248 35 2552
Fragments arranged by size:
2552 414 401 248 237 35
BanII G_rGCy'C
Cuts at: 278 381 460 683 842 901 1931 278
Size: 103 79 223 159 59 1030 2234
Fragments arranged by size:
2234 1030 223 159 103 79 59
BbeI G_GCGC'C
Cuts at: 269 1321 1569 269
Size: 1052 248 2587
Fragments arranged by size:
2587 1052 248
BbsI GAAGACnn'nnnn_
Cuts at: 426 617 426
Size: 191 3696
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3853
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BfrBI ATG'CAT
Cuts at: 877 877
Size: 3887
BglI GCCn_nnn'nGGC
Cuts at: 245 245
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 381 661 669 683 842 1512 1605 2661 381
Size: 280 8 14 159 670 93 1056 1607
Fragments arranged by size:
1607 1056 670 280 159 93 14 8
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1180 1515 3659 1099
Size: 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 484 135
Size: 349 3538
Bpu10I CC'TnA_GC
Cuts at: 192 454 192
Size: 262 3625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 596 724 3591 596
Size: 128 2867 892
Fragments arranged by size:
2867 892 128
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 266 431 784 1318 1566 2268 3764 266
Size: 165 353 534 248 702 1496 389
Fragments arranged by size:
1496 702 534 389 353 248 165
BsaWI w'CCGG_w
Cuts at: 1076 1597 2275 2549 2696 3107 1076
Size: 521 678 274 147 411 1856
Fragments arranged by size:
1856 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 160 190 160
Size: 30 3857
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 108 111 108
Size: 3 3884
BseYI C'CCAG_C
Cuts at: 566 606 816 1312 2070 2647 3143 566
Size: 40 210 496 758 577 496 1310
Fragments arranged by size:
1310 758 577 496 496 210 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 3887
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 901 1679 1869 2661 901
Size: 778 190 792 2127
Fragments arranged by size:
2127 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 278 381 460 661 669 683 842 901 1512
Size: 103 79 201 8 14 159 59 611
Cuts at: 1512 1605 1679 1869 1931 2661 278
Size: 93 74 190 62 730 1504
Fragments arranged by size:
1504 730 611 201 190 159 103 93
79 74 62 59 14 8
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 402 937 1151 1453 1834 402
Size: 535 214 302 381 2455
Fragments arranged by size:
2455 535 381 302 214
BsrBI CCG'CTC
Cuts at: 50 468 2179 2233 3717 3882 50
Size: 418 1711 54 1484 165 55
Fragments arranged by size:
1711 1484 418 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 806 1885 2066 2275 806
Size: 1079 181 209 2418
Fragments arranged by size:
2418 1079 209 181
BssSI C'ACGA_G
Cuts at: 131 2158 2516 131
Size: 2027 358 1502
Fragments arranged by size:
2027 1502 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 281 281
Size: 3887
BtgI C'CryG_G
Cuts at: 164 341 404 833 164
Size: 177 63 429 3218
Fragments arranged by size:
3218 429 177 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DraIII CAC_nnn'GTG
Cuts at: 133 133
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 344 479 1472 1646 2037 2064 3409 344
Size: 135 993 174 391 27 1345 822
Fragments arranged by size:
1345 993 822 391 174 135 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 466 1910 2120 466
Size: 1444 210 2233
Fragments arranged by size:
2233 1444 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 484 1712 2144 2891 3159 135
Size: 349 1228 432 747 268 863
Fragments arranged by size:
1228 863 747 432 349 268
EcoNI CCTnn'n_nnAGG
Cuts at: 800 800
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 311 378 561 680 741 838 839 846 1026
Size: 67 183 119 61 97 1 7 180
Cuts at: 1026 311
Size: 3172
Fragments arranged by size:
3172 183 180 119 97 67 61 7
1
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FseI GG_CCGG'CC
Cuts at: 810 810
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 269 1321 1569 2591 3570 269
Size: 1052 248 1022 979 586
Fragments arranged by size:
1052 1022 979 586 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 1729 1759 1761 1791 160
Size: 32 1537 30 2 30 2256
Fragments arranged by size:
2256 1537 32 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 265 1317 1565 265
Size: 1052 248 2587
Fragments arranged by size:
2587 1052 248
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 481 1648 481
Size: 1167 2720
MslI CAynn'nnrTG
Cuts at: 1876 2003 1876
Size: 127 3760
MspA1I CmG'CkG
Cuts at: 188 570 651 998 1312 1672 2685 2930 188
Size: 382 81 347 314 360 1013 245 1145
Fragments arranged by size:
1145 1013 382 360 347 314 245 81
NaeI GCC'GGC
Cuts at: 808 2068 808
Size: 1260 2627
NarI GG'CG_CC
Cuts at: 266 1318 1566 266
Size: 1052 248 2587
Fragments arranged by size:
2587 1052 248
NcoI C'CATG_G
Cuts at: 341 404 341
Size: 63 3824
NgoMIV G'CCGG_C
Cuts at: 806 2066 806
Size: 1260 2627
NsiI A_TGCA'T
Cuts at: 879 879
Size: 3887
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PflMI CCAn_nnn'nTGG
Cuts at: 133 133
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PpuMI rG'GwC_Cy
Cuts at: 311 561 311
Size: 250 3637
PspOMI G'GGCC_C
Cuts at: 377 679 838 377
Size: 302 159 3426
Fragments arranged by size:
3426 302 159
PstI C_TGCA'G
Cuts at: 650 934 1619 650
Size: 284 685 2918
Fragments arranged by size:
2918 685 284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 188 570 651 1312 1672 188
Size: 382 81 661 360 2403
Fragments arranged by size:
2403 661 382 360 81
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SexAI A'CCwGG_T
Cuts at: 517 517
Size: 3887
SfcI C'TryA_G
Cuts at: 33 646 930 1615 2608 2799 33
Size: 613 284 685 993 191 1121
Fragments arranged by size:
1121 993 685 613 284 191
SfoI GGC'GCC
Cuts at: 267 1319 1567 267
Size: 1052 248 2587
Fragments arranged by size:
2587 1052 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 233 251 271 844 909 233
Size: 18 20 573 65 3211
Fragments arranged by size:
3211 573 65 20 18
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
SrfI GCCC'GGGC
Cuts at: 251 251
Size: 3887
StuI AGG'CCT
Cuts at: 216 216
Size: 3887
StyI C'CwwG_G
Cuts at: 341 404 422 661 1021 1375 341
Size: 63 18 239 360 354 2853
Fragments arranged by size:
2853 360 354 239 63 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1470 1603 2062 2271 1470
Size: 133 459 209 3086
Fragments arranged by size:
3086 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 1382
Size: 3887
XmaI C'CCGG_G
Cuts at: 231 249 269 842 907 231
Size: 18 20 573 65 3211
Fragments arranged by size:
3211 573 65 20 18
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI