Restriction map for
pF1KB7541
(Circular) (Six-base) MAPSORT of: pF1KB7541.seq Check: 1069 from: 1 to: 3647
pF1KB7541 3647 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 685 685
Size: 3647
Acc65I G'GTAC_C
Cuts at: 663 663
Size: 3647
AclI AA'CG_TT
Cuts at: 641 3487 641
Size: 2846 801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 557 1472 1904 2651 2919 357
Size: 200 915 432 747 268 1085
Fragments arranged by size:
1085 915 747 432 268 200
AflIII A'CryG_T
Cuts at: 999 2103 3080 999
Size: 1104 977 1566
Fragments arranged by size:
1566 1104 977
AgeI A'CCGG_T
Cuts at: 326 2035 326
Size: 1709 1938
AlwNI CAG_nnn'CTG
Cuts at: 270 952 2519 270
Size: 682 1567 1398
Fragments arranged by size:
1567 1398 682
ApaI G_GGCC'C
Cuts at: 152 164 152
Size: 12 3635
ApaLI G'TGCA_C
Cuts at: 2417 2417
Size: 3647
ApoI r'AATT_y
Cuts at: 651 3000 3256 651
Size: 2349 256 1042
Fragments arranged by size:
2349 1042 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
AvaI C'yCGr_G
Cuts at: 145 182 667 145
Size: 37 485 3125
Fragments arranged by size:
3125 485 37
BamHI G'GATC_C
Cuts at: 426 672 426
Size: 246 3401
BanI G'GyrC_C
Cuts at: 141 663 1077 1325 1360 141
Size: 522 414 248 35 2428
Fragments arranged by size:
2428 522 414 248 35
BanII G_rGCy'C
Cuts at: 152 164 661 1691 152
Size: 12 497 1030 2108
Fragments arranged by size:
2108 1030 497 12
BbeI G_GCGC'C
Cuts at: 145 1081 1329 145
Size: 936 248 2463
Fragments arranged by size:
2463 936 248
BciVI GTATCCnnnnn_n'
Cuts at: 1545 2306 3647 1545
Size: 761 1341 1545
Fragments arranged by size:
1545 1341 761
BclI T'GATC_A
Cuts at: 1167 1167
Size: 3647
BglI GCCn_nnn'nGGC
Cuts at: 628 628
Size: 3647
BglII A'GATC_T
Cuts at: 257 1162 3199 257
Size: 905 2037 705
Fragments arranged by size:
2037 905 705
BlpI GC'TnA_GC
Cuts at: 170 759 170
Size: 589 3058
Bme1580I G_kGCm'C
Cuts at: 152 164 1272 1365 2421 152
Size: 12 1108 93 1056 1378
Fragments arranged by size:
1378 1108 1056 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 859 940 1275 3419 859
Size: 81 335 2144 1087
Fragments arranged by size:
2144 1087 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1702 2194 2492 823
Size: 879 492 298 1978
Fragments arranged by size:
1978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3351 3351
Size: 3647
BsaAI yAC'GTr
Cuts at: 1000 1000
Size: 3647
BsaBI GATnn'nnATC
Cuts at: 6 1161 3198 6
Size: 1155 2037 455
Fragments arranged by size:
2037 1155 455
BsaHI Gr'CG_yC
Cuts at: 142 199 1078 1326 2028 3524 142
Size: 57 879 248 702 1496 265
Fragments arranged by size:
1496 879 702 265 248 57
BsaWI w'CCGG_w
Cuts at: 326 836 1357 2035 2309 2456 2867 326
Size: 510 521 678 274 147 411 1106
Fragments arranged by size:
1106 678 521 510 411 274 147
BseYI C'CCAG_C
Cuts at: 1072 1830 2407 2903 1072
Size: 758 577 496 1816
Fragments arranged by size:
1816 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 250
Size: 3647
BsiEI CG_ry'CG
Cuts at: 104 1189 1235 2443 104
Size: 1085 46 1208 1308
Fragments arranged by size:
1308 1208 1085 46
BsiHKAI G_wGCw'C
Cuts at: 661 1439 1629 2421 661
Size: 778 190 792 1887
Fragments arranged by size:
1887 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 152 164 661 1272 1365 1439 1629 1691 2421
Size: 12 497 611 93 74 190 62 730
Cuts at: 2421 152
Size: 1378
Fragments arranged by size:
1378 730 611 497 190 93 74 62
12
BspEI T'CCGG_A
Cuts at: 2867 2867
Size: 3647
BspHI T'CATG_A
Cuts at: 2823 2823
Size: 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 697 911 1213 1594 697
Size: 214 302 381 2750
Fragments arranged by size:
2750 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1939 1993 3477 3642 50
Size: 1889 54 1484 165 55
Fragments arranged by size:
1889 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 411 638 1559 411
Size: 227 921 2499
Fragments arranged by size:
2499 921 227
BsrFI r'CCGG_y
Cuts at: 326 1645 1826 2035 326
Size: 1319 181 209 1938
Fragments arranged by size:
1938 1319 209 181
BssSI C'ACGA_G
Cuts at: 1918 2276 1918
Size: 358 3289
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 1024
Size: 3647
BstBI TT'CG_AA
Cuts at: 2008 2008
Size: 3647
BstYI r'GATC_y
Cuts at: 1 257 426 672 1139 1162 1497 1743 2755
Size: 256 169 246 467 23 335 246 1012
Cuts at: 2755 2841 2853 3199 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 256 246 246
169 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 271 271
Size: 3647
BtgI C'CryG_G
Cuts at: 251 251
Size: 3647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1597 1778 1941 1597
Size: 181 163 3303
Fragments arranged by size:
3303 181 163
BtsI GCAGTG_nn'
Cuts at: 890 890
Size: 3647
DraI TTT'AAA
Cuts at: 646 646
Size: 3647
DrdI GACnn_nn'nnGTC
Cuts at: 1353 2211 1353
Size: 858 2789
EaeI y'GGCC_r
Cuts at: 125 1232 1406 1797 1824 3169 125
Size: 1107 174 391 27 1345 603
Fragments arranged by size:
1345 1107 603 391 174 27
EagI C'GGCC_G
Cuts at: 1232 1232
Size: 3647
EarI CTCTTCn'nnn_
Cuts at: 376 512 1670 1880 376
Size: 136 1158 210 2143
Fragments arranged by size:
2143 1158 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1855 2165 2311 3457 1855
Size: 310 146 1146 2045
Fragments arranged by size:
2045 1146 310 146
EcoICRI GAG'CTC
Cuts at: 659 659
Size: 3647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 557 1472 1904 2651 2919 357
Size: 200 915 432 747 268 1085
Fragments arranged by size:
1085 915 747 432 268 200
EcoO109I rG'GnC_Cy
Cuts at: 149 161 786 149
Size: 12 625 3010
Fragments arranged by size:
3010 625 12
EcoRI G'AATT_C
Cuts at: 651 651
Size: 3647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1666 1666
Size: 3647
FspI TGC'GCA
Cuts at: 1428 1428
Size: 3647
HaeII r_GCGC'y
Cuts at: 145 1081 1329 2351 3330 145
Size: 936 248 1022 979 462
Fragments arranged by size:
1022 979 936 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1489 1519 1521 1551 1489
Size: 30 2 30 3585
Fragments arranged by size:
3585 30 30 2
HincII GTy'rAC
Cuts at: 192 332 686 192
Size: 140 354 3153
Fragments arranged by size:
3153 354 140
HpaI GTT'AAC
Cuts at: 332 332
Size: 3647
KasI G'GCGC_C
Cuts at: 141 1077 1325 141
Size: 936 248 2463
Fragments arranged by size:
2463 936 248
KpnI G_GTAC'C
Cuts at: 667 667
Size: 3647
MluI A'CGCG_T
Cuts at: 3080 3080
Size: 3647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2502 2318
Size: 184 3463
MscI TGG'CCA
Cuts at: 1408 1408
Size: 3647
MslI CAynn'nnrTG
Cuts at: 1636 1763 1636
Size: 127 3520
MspA1I CmG'CkG
Cuts at: 285 573 758 1072 1432 2445 2690 285
Size: 288 185 314 360 1013 245 1242
Fragments arranged by size:
1242 1013 360 314 288 245 185
NaeI GCC'GGC
Cuts at: 1828 1828
Size: 3647
NarI GG'CG_CC
Cuts at: 142 1078 1326 142
Size: 936 248 2463
Fragments arranged by size:
2463 936 248
NcoI C'CATG_G
Cuts at: 251 251
Size: 3647
NdeI CA'TA_TG
Cuts at: 456 456
Size: 3647
NgoMIV G'CCGG_C
Cuts at: 1826 1826
Size: 3647
NspI r_CATG'y
Cuts at: 282 700 1200 2107 282
Size: 418 500 907 1822
Fragments arranged by size:
1822 907 500 418
PciI A'CATG_T
Cuts at: 2103 2103
Size: 3647
PmeI GTTT'AAAC
Cuts at: 646 646
Size: 3647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 3615
PspOMI G'GGCC_C
Cuts at: 148 160 148
Size: 12 3635
PstI C_TGCA'G
Cuts at: 694 1379 694
Size: 685 2962
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3647
PvuII CAG'CTG
Cuts at: 285 573 1072 1432 285
Size: 288 499 360 2500
Fragments arranged by size:
2500 499 360 288
RsrII CG'GwC_CG
Cuts at: 1842 1842
Size: 3647
SacI G_AGCT'C
Cuts at: 661 661
Size: 3647
SalI G'TCGA_C
Cuts at: 684 684
Size: 3647
SapI GCTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
SbfI CC_TGCA'GG
Cuts at: 694 694
Size: 3647
ScaI AGT'ACT
Cuts at: 3164 3164
Size: 3647
SfcI C'TryA_G
Cuts at: 33 340 690 1375 2368 2559 33
Size: 307 350 685 993 191 1121
Fragments arranged by size:
1121 993 685 350 307 191
SfoI GGC'GCC
Cuts at: 143 1079 1327 143
Size: 936 248 2463
Fragments arranged by size:
2463 936 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
SmaI CCC'GGG
Cuts at: 147 184 669 147
Size: 37 485 3125
Fragments arranged by size:
3125 485 37
SmlI C'TyrA_G
Cuts at: 802 1717 2209 2471 802
Size: 915 492 262 1978
Fragments arranged by size:
1978 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3647
SphI G_CATG'C
Cuts at: 700 1200 700
Size: 500 3147
SrfI GCCC'GGGC
Cuts at: 147 147
Size: 3647
StyI C'CwwG_G
Cuts at: 251 781 1135 251
Size: 530 354 2763
Fragments arranged by size:
2763 530 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1230 1363 1822 2031 1230
Size: 133 459 209 2846
Fragments arranged by size:
2846 459 209 133
TatI w'GTAC_w
Cuts at: 3162 3162
Size: 3647
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3615
Tth111I GACn'n_nGTC
Cuts at: 1444 1444
Size: 3647
XbaI T'CTAG_A
Cuts at: 678 678
Size: 3647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 1142 120
Size: 1022 2625
XmaI C'CCGG_G
Cuts at: 145 182 667 145
Size: 37 485 3125
Fragments arranged by size:
3125 485 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI