Restriction map for
pF1KB7542
(Circular) (Six-base) MAPSORT of: pF1KB7542.seq Check: 9109 from: 1 to: 3887
pF1KB7542 3887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 761 761
Size: 3887
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 778 1712 2144 2891 3159 460
Size: 318 934 432 747 268 1188
Fragments arranged by size:
1188 934 747 432 318 268
AflIII A'CryG_T
Cuts at: 230 317 1239 2343 3320 230
Size: 87 922 1104 977 797
Fragments arranged by size:
1104 977 922 797 87
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AleI CACnn'nnGTG
Cuts at: 652 652
Size: 3887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 395 427 395
Size: 32 3855
AlwNI CAG_nnn'CTG
Cuts at: 1192 2759 1192
Size: 1567 2320
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 253 891 3240 3496 253
Size: 638 2349 256 644
Fragments arranged by size:
2349 644 638 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 907 907
Size: 3887
BamHI G'GATC_C
Cuts at: 339 912 339
Size: 573 3314
BanI G'GyrC_C
Cuts at: 133 903 1317 1565 1600 133
Size: 770 414 248 35 2420
Fragments arranged by size:
2420 770 414 248 35
BanII G_rGCy'C
Cuts at: 635 901 1931 635
Size: 266 1030 2591
Fragments arranged by size:
2591 1030 266
BbeI G_GCGC'C
Cuts at: 137 1321 1569 137
Size: 1184 248 2455
Fragments arranged by size:
2455 1184 248
BbvCI CC'TCA_GC
Cuts at: 194 857 194
Size: 663 3224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 561 595 90
Size: 34 437 34 3382
Fragments arranged by size:
3382 437 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 539 1407 539
Size: 868 3019
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 629 999 629
Size: 370 3517
Bme1580I G_kGCm'C
Cuts at: 1512 1605 2661 1512
Size: 93 1056 2738
Fragments arranged by size:
2738 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1180 1515 3659 1099
Size: 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 81
BmtI G_CTAG'C
Cuts at: 208 208
Size: 3887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 892 2254 892
Size: 1362 2525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 268
Size: 3887
Bpu10I CC'TnA_GC
Cuts at: 194 857 194
Size: 663 3224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 715 3591 715
Size: 2876 1011
BsaAI yAC'GTr
Cuts at: 684 1240 684
Size: 556 3331
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 134 1318 1566 2268 3764 134
Size: 1184 248 702 1496 257
Fragments arranged by size:
1496 1184 702 257 248
BsaWI w'CCGG_w
Cuts at: 281 1076 1597 2275 2549 2696 3107 281
Size: 795 521 678 274 147 411 1061
Fragments arranged by size:
1061 795 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 823 853 823
Size: 30 3857
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 874 874
Size: 3887
BseYI C'CCAG_C
Cuts at: 378 1312 2070 2647 3143 378
Size: 934 758 577 496 1122
Fragments arranged by size:
1122 934 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 325
Size: 3887
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 635 901 1679 1869 2661 635
Size: 266 778 190 792 1861
Fragments arranged by size:
1861 792 778 266 190
Bsp1286I G_dGCh'C
Cuts at: 635 901 1512 1605 1679 1869 1931 2661 635
Size: 266 611 93 74 190 62 730 1861
Fragments arranged by size:
1861 730 611 266 190 93 74 62
BspEI T'CCGG_A
Cuts at: 281 3107 281
Size: 2826 1061
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 761 937 1151 1453 1834 761
Size: 176 214 302 381 2814
Fragments arranged by size:
2814 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 1885 2066 2275 1885
Size: 181 209 3497
Fragments arranged by size:
3497 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstXI CCAn_nnnn'nTGG
Cuts at: 336 336
Size: 3887
BstYI r'GATC_y
Cuts at: 1 278 339 912 1379 1402 1737 1983 2995
Size: 277 61 573 467 23 335 246 1012
Cuts at: 2995 3081 3093 3439 1
Size: 86 12 346 449
Fragments arranged by size:
1012 573 467 449 346 335 277 246
86 61 23 12
BtgI C'CryG_G
Cuts at: 117 650 117
Size: 533 3354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 123 1837 2018 2181 123
Size: 1714 181 163 1829
Fragments arranged by size:
1829 1714 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DraIII CAC_nnn'GTG
Cuts at: 651 651
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 1472 1646 2037 2064 3409 1472
Size: 174 391 27 1345 1950
Fragments arranged by size:
1950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 829 1910 2120 829
Size: 1081 210 2596
Fragments arranged by size:
2596 1081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 633 899 633
Size: 266 3621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 460 778 1712 2144 2891 3159 268
Size: 192 318 934 432 747 268 996
Fragments arranged by size:
996 934 747 432 318 268 192
EcoNI CCTnn'n_nnAGG
Cuts at: 757 757
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 789 1026 789
Size: 237 3650
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 727 1906 727
Size: 1179 2708
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 137 439 1321 1569 2591 3570 137
Size: 302 882 248 1022 979 454
Fragments arranged by size:
1022 979 882 454 302 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 823 855 1729 1759 1761 1791 170
Size: 32 621 32 874 30 2 30 2266
Fragments arranged by size:
2266 874 621 32 32 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 133 1317 1565 133
Size: 1184 248 2455
Fragments arranged by size:
2455 1184 248
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 2558 2742 206
Size: 2352 184 1351
Fragments arranged by size:
2352 1351 184
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 334 449 592 652 1876 2003 334
Size: 115 143 60 1224 127 2218
Fragments arranged by size:
2218 1224 143 127 115 60
MspA1I CmG'CkG
Cuts at: 119 345 628 663 856 998 1312 1672 2685
Size: 226 283 35 193 142 314 360 1013
Cuts at: 2685 2930 119
Size: 245 1076
Fragments arranged by size:
1076 1013 360 314 283 245 226 193
142 35
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 134 1318 1566 134
Size: 1184 248 2455
Fragments arranged by size:
2455 1184 248
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NheI G'CTAG_C
Cuts at: 204 204
Size: 3887
NspI r_CATG'y
Cuts at: 234 321 940 1440 2347 234
Size: 87 619 500 907 1774
Fragments arranged by size:
1774 907 619 500 87
PasI CC'CwG_GG
Cuts at: 793 793
Size: 3887
PciI A'CATG_T
Cuts at: 230 317 2343 230
Size: 87 2026 1774
Fragments arranged by size:
2026 1774 87
PfoI T'CCnGG_A
Cuts at: 311 311
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PstI C_TGCA'G
Cuts at: 662 934 1619 662
Size: 272 685 2930
Fragments arranged by size:
2930 685 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 628 663 856 1312 1672 628
Size: 35 193 456 360 2843
Fragments arranged by size:
2843 456 360 193 35
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 635 901 635
Size: 266 3621
SacII CC_GC'GG
Cuts at: 120 120
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 658 930 1615 2608 2799 33
Size: 625 272 685 993 191 1121
Fragments arranged by size:
1121 993 685 625 272 191
SfoI GGC'GCC
Cuts at: 135 1319 1567 135
Size: 1184 248 2455
Fragments arranged by size:
2455 1184 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 1021 1375 1021
Size: 354 3533
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 739 1470 1603 2062 2271 739
Size: 731 133 459 209 2355
Fragments arranged by size:
2355 731 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 1382
Size: 3887
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BfrBI BglI BmgBI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI