Restriction map for
pF1KB7543
(Circular) (Six-base) MAPSORT of: pF1KB7543.seq Check: 1550 from: 1 to: 3659
pF1KB7543 3659 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 365 697 365
Size: 332 3327
Acc65I G'GTAC_C
Cuts at: 675 675
Size: 3659
AclI AA'CG_TT
Cuts at: 3499 3499
Size: 3659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 1484 1916 2663 2931 186
Size: 1298 432 747 268 914
Fragments arranged by size:
1298 914 747 432 268
AflIII A'CryG_T
Cuts at: 1011 2115 3092 1011
Size: 1104 977 1578
Fragments arranged by size:
1578 1104 977
AgeI A'CCGG_T
Cuts at: 2047 2047
Size: 3659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 530 530
Size: 3659
AlwNI CAG_nnn'CTG
Cuts at: 621 631 964 2531 621
Size: 10 333 1567 1749
Fragments arranged by size:
1749 1567 333 10
ApaLI G'TGCA_C
Cuts at: 2429 2429
Size: 3659
ApoI r'AATT_y
Cuts at: 562 663 3012 3268 562
Size: 101 2349 256 953
Fragments arranged by size:
2349 953 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 3659
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
AvaI C'yCGr_G
Cuts at: 679 679
Size: 3659
BamHI G'GATC_C
Cuts at: 684 684
Size: 3659
BanI G'GyrC_C
Cuts at: 675 1089 1337 1372 675
Size: 414 248 35 2962
Fragments arranged by size:
2962 414 248 35
BanII G_rGCy'C
Cuts at: 504 673 1703 504
Size: 169 1030 2460
Fragments arranged by size:
2460 1030 169
BbeI G_GCGC'C
Cuts at: 1093 1341 1093
Size: 248 3411
BbsI GAAGACnn'nnnn_
Cuts at: 646 646
Size: 3659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3625
BciVI GTATCCnnnnn_n'
Cuts at: 1557 2318 3659 1557
Size: 761 1341 1557
Fragments arranged by size:
1557 1341 761
BclI T'GATC_A
Cuts at: 1179 1179
Size: 3659
BfrBI ATG'CAT
Cuts at: 517 517
Size: 3659
BglI GCCn_nnn'nGGC
Cuts at: 257 257
Size: 3659
BglII A'GATC_T
Cuts at: 1174 3211 1174
Size: 2037 1622
BlpI GC'TnA_GC
Cuts at: 169 771 169
Size: 602 3057
Bme1580I G_kGCm'C
Cuts at: 125 1284 1377 2433 125
Size: 1159 93 1056 1351
Fragments arranged by size:
1351 1159 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 871 952 1287 3431 871
Size: 81 335 2144 1099
Fragments arranged by size:
2144 1099 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 3659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 502 289
Size: 213 3446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1714 2206 2504 835
Size: 879 492 298 1990
Fragments arranged by size:
1990 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 587 3363 587
Size: 2776 883
BsaAI yAC'GTr
Cuts at: 1012 1012
Size: 3659
BsaBI GATnn'nnATC
Cuts at: 6 1173 3210 6
Size: 1167 2037 455
Fragments arranged by size:
2037 1167 455
BsaHI Gr'CG_yC
Cuts at: 1090 1338 2040 3536 1090
Size: 248 702 1496 1213
Fragments arranged by size:
1496 1213 702 248
BsaWI w'CCGG_w
Cuts at: 848 1369 2047 2321 2468 2879 848
Size: 521 678 274 147 411 1628
Fragments arranged by size:
1628 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 413 443 413
Size: 30 3629
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 508 166
Size: 342 3317
BseYI C'CCAG_C
Cuts at: 503 1084 1842 2419 2915 503
Size: 581 758 577 496 1247
Fragments arranged by size:
1247 758 581 577 496
BsiEI CG_ry'CG
Cuts at: 104 261 1201 1247 2455 104
Size: 157 940 46 1208 1308
Fragments arranged by size:
1308 1208 940 157 46
BsiHKAI G_wGCw'C
Cuts at: 673 1451 1641 2433 673
Size: 778 190 792 1899
Fragments arranged by size:
1899 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 125 504 673 1284 1377 1451 1641 1703 2433
Size: 379 169 611 93 74 190 62 730
Cuts at: 2433 125
Size: 1351
Fragments arranged by size:
1351 730 611 379 190 169 93 74
62
BspEI T'CCGG_A
Cuts at: 2879 2879
Size: 3659
BspHI T'CATG_A
Cuts at: 2835 2835
Size: 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 709 923 1225 1606 709
Size: 214 302 381 2762
Fragments arranged by size:
2762 381 302 214
BsrBI CCG'CTC
Cuts at: 50 263 1951 2005 3489 3654 50
Size: 213 1688 54 1484 165 55
Fragments arranged by size:
1688 1484 213 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1571 1571
Size: 3659
BsrFI r'CCGG_y
Cuts at: 209 1657 1838 2047 209
Size: 1448 181 209 1821
Fragments arranged by size:
1821 1448 209 181
BssSI C'ACGA_G
Cuts at: 217 1930 2288 217
Size: 1713 358 1588
Fragments arranged by size:
1713 1588 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1036 1036
Size: 3659
BstBI TT'CG_AA
Cuts at: 2020 2020
Size: 3659
BstYI r'GATC_y
Cuts at: 1 684 1151 1174 1509 1755 2767 2853 2865
Size: 683 467 23 335 246 1012 86 12
Cuts at: 2865 3211 1
Size: 346 449
Fragments arranged by size:
1012 683 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1609 1790 1953 1609
Size: 181 163 3315
Fragments arranged by size:
3315 181 163
BtsI GCAGTG_nn'
Cuts at: 902 902
Size: 3659
DraI TTT'AAA
Cuts at: 658 658
Size: 3659
DrdI GACnn_nn'nnGTC
Cuts at: 1365 2223 1365
Size: 858 2801
EaeI y'GGCC_r
Cuts at: 258 281 317 1244 1418 1809 1836 3181 258
Size: 23 36 927 174 391 27 1345 736
Fragments arranged by size:
1345 927 736 391 174 36 27 23
EagI C'GGCC_G
Cuts at: 258 1244 258
Size: 986 2673
EarI CTCTTCn'nnn_
Cuts at: 533 1682 1892 533
Size: 1149 210 2300
Fragments arranged by size:
2300 1149 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1867 2177 2323 3469 1867
Size: 310 146 1146 2057
Fragments arranged by size:
2057 1146 310 146
EcoICRI GAG'CTC
Cuts at: 671 671
Size: 3659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 289 502 1484 1916 2663 2931 186
Size: 103 213 982 432 747 268 914
Fragments arranged by size:
982 914 747 432 268 213 103
EcoO109I rG'GnC_Cy
Cuts at: 798 798
Size: 3659
EcoRI G'AATT_C
Cuts at: 663 663
Size: 3659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1678 1678
Size: 3659
FspI TGC'GCA
Cuts at: 1440 1440
Size: 3659
HaeII r_GCGC'y
Cuts at: 1093 1341 2363 3342 1093
Size: 248 1022 979 1410
Fragments arranged by size:
1410 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 347 379 1501 1531 1533 1563 247
Size: 32 68 32 1122 30 2 30 2343
Fragments arranged by size:
2343 1122 68 32 32 30 30 2
HincII GTy'rAC
Cuts at: 366 698 366
Size: 332 3327
KasI G'GCGC_C
Cuts at: 1089 1337 1089
Size: 248 3411
KpnI G_GTAC'C
Cuts at: 679 679
Size: 3659
MluI A'CGCG_T
Cuts at: 3092 3092
Size: 3659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2514 2330
Size: 184 3475
MscI TGG'CCA
Cuts at: 283 1420 283
Size: 1137 2522
MslI CAynn'nnrTG
Cuts at: 1648 1775 1648
Size: 127 3532
MspA1I CmG'CkG
Cuts at: 323 618 770 1084 1444 2457 2702 323
Size: 295 152 314 360 1013 245 1280
Fragments arranged by size:
1280 1013 360 314 295 245 152
NaeI GCC'GGC
Cuts at: 1840 1840
Size: 3659
NarI GG'CG_CC
Cuts at: 1090 1338 1090
Size: 248 3411
NgoMIV G'CCGG_C
Cuts at: 1838 1838
Size: 3659
NsiI A_TGCA'T
Cuts at: 519 519
Size: 3659
NspI r_CATG'y
Cuts at: 712 1212 2119 712
Size: 500 907 2252
Fragments arranged by size:
2252 907 500
PciI A'CATG_T
Cuts at: 2115 2115
Size: 3659
PflMI CCAn_nnn'nTGG
Cuts at: 462 462
Size: 3659
PmeI GTTT'AAAC
Cuts at: 658 658
Size: 3659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 3627
PstI C_TGCA'G
Cuts at: 706 1391 706
Size: 685 2974
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3659
PvuII CAG'CTG
Cuts at: 323 618 1084 1444 323
Size: 295 466 360 2538
Fragments arranged by size:
2538 466 360 295
RsrII CG'GwC_CG
Cuts at: 1854 1854
Size: 3659
SacI G_AGCT'C
Cuts at: 673 673
Size: 3659
SalI G'TCGA_C
Cuts at: 364 696 364
Size: 332 3327
SapI GCTCTTCn'nnn_
Cuts at: 1682 1892 1682
Size: 210 3449
SbfI CC_TGCA'GG
Cuts at: 706 706
Size: 3659
ScaI AGT'ACT
Cuts at: 3176 3176
Size: 3659
SfcI C'TryA_G
Cuts at: 33 702 1387 2380 2571 33
Size: 669 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 191
SfoI GGC'GCC
Cuts at: 1091 1339 1091
Size: 248 3411
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
SmaI CCC'GGG
Cuts at: 681 681
Size: 3659
SmlI C'TyrA_G
Cuts at: 814 1729 2221 2483 814
Size: 915 492 262 1990
Fragments arranged by size:
1990 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3659
SphI G_CATG'C
Cuts at: 712 1212 712
Size: 500 3159
StuI AGG'CCT
Cuts at: 532 532
Size: 3659
StyI C'CwwG_G
Cuts at: 793 1147 793
Size: 354 3305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1242 1375 1834 2043 1242
Size: 133 459 209 2858
Fragments arranged by size:
2858 459 209 133
TatI w'GTAC_w
Cuts at: 191 3174 191
Size: 2983 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 3627
Tth111I GACn'n_nGTC
Cuts at: 1456 1456
Size: 3659
XbaI T'CTAG_A
Cuts at: 690 690
Size: 3659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 463 1154 463
Size: 691 2968
XmaI C'CCGG_G
Cuts at: 679 679
Size: 3659
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI