(Circular) (Six-base) MAPSORT of: pF1KB7546.seq Check: 2118 from: 1 to: 3905 pF1KB7546 3905 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 233 233 Size: 3905 AccI GT'mk_AC Cuts at: 943 943 Size: 3905 Acc65I G'GTAC_C Cuts at: 921 921 Size: 3905 AclI AA'CG_TT Cuts at: 3745 3745 Size: 3905 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 440 1730 2162 2909 3177 440 Size: 1290 432 747 268 1168 Fragments arranged by size: 1290 1168 747 432 268 AflIII A'CryG_T Cuts at: 1257 2361 3338 1257 Size: 1104 977 1824 Fragments arranged by size: 1824 1104 977 AgeI A'CCGG_T Cuts at: 2293 2293 Size: 3905 AlwNI CAG_nnn'CTG Cuts at: 1210 2777 1210 Size: 1567 2338 ApaI G_GGCC'C Cuts at: 783 783 Size: 3905 ApaLI G'TGCA_C Cuts at: 2675 2675 Size: 3905 ApoI r'AATT_y Cuts at: 909 3258 3514 909 Size: 2349 256 1300 Fragments arranged by size: 2349 1300 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3905 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3905 AvaI C'yCGr_G Cuts at: 925 925 Size: 3905 BamHI G'GATC_C Cuts at: 930 930 Size: 3905 BanI G'GyrC_C Cuts at: 303 607 921 1335 1583 1618 303 Size: 304 314 414 248 35 2590 Fragments arranged by size: 2590 414 314 304 248 35 BanII G_rGCy'C Cuts at: 783 919 1949 783 Size: 136 1030 2739 Fragments arranged by size: 2739 1030 136 BbeI G_GCGC'C Cuts at: 307 1339 1587 307 Size: 1032 248 2625 Fragments arranged by size: 2625 1032 248 BbvCI CC'TCA_GC Cuts at: 598 598 Size: 3905 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 466 500 90 Size: 34 342 34 3495 Fragments arranged by size: 3495 342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1803 2564 3905 1803 Size: 761 1341 1803 Fragments arranged by size: 1803 1341 761 BclI T'GATC_A Cuts at: 1425 1425 Size: 3905 BglII A'GATC_T Cuts at: 1420 3457 1420 Size: 2037 1868 BlpI GC'TnA_GC Cuts at: 1017 1017 Size: 3905 Bme1580I G_kGCm'C Cuts at: 515 612 783 1530 1623 2679 515 Size: 97 171 747 93 1056 1741 Fragments arranged by size: 1741 1056 747 171 97 93 BmrI ACTGGGnnnn_n' Cuts at: 1117 1198 1533 3677 1117 Size: 81 335 2144 1345 Fragments arranged by size: 2144 1345 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 390 2272 390 Size: 1882 2023 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 328 535 772 328 Size: 207 237 3461 Fragments arranged by size: 3461 237 207 Bpu10I CC'TnA_GC Cuts at: 598 885 598 Size: 287 3618 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 598 1081 1960 2452 2750 598 Size: 483 879 492 298 1753 Fragments arranged by size: 1753 879 492 483 298 BsaI GGTCTCn'nnnn_ Cuts at: 3609 3609 Size: 3905 BsaAI yAC'GTr Cuts at: 1258 1258 Size: 3905 BsaBI GATnn'nnATC Cuts at: 6 622 1419 3456 6 Size: 616 797 2037 455 Fragments arranged by size: 2037 797 616 455 BsaHI Gr'CG_yC Cuts at: 304 1336 1584 2286 3782 304 Size: 1032 248 702 1496 427 Fragments arranged by size: 1496 1032 702 427 248 BsaWI w'CCGG_w Cuts at: 246 704 1094 1615 2293 2567 2714 3125 246 Size: 458 390 521 678 274 147 411 1026 Fragments arranged by size: 1026 678 521 458 411 390 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 438 438 Size: 3905 BseYI C'CCAG_C Cuts at: 314 407 415 527 1330 2088 2665 3161 314 Size: 93 8 112 803 758 577 496 1058 Fragments arranged by size: 1058 803 758 577 496 112 93 8 BsiEI CG_ry'CG Cuts at: 104 1447 1493 2701 104 Size: 1343 46 1208 1308 Fragments arranged by size: 1343 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 834 919 1697 1887 2679 834 Size: 85 778 190 792 2060 Fragments arranged by size: 2060 792 778 190 85 Bsp1286I G_dGCh'C Cuts at: 515 612 783 834 919 1530 1623 1697 1887 Size: 97 171 51 85 611 93 74 190 Cuts at: 1887 1949 2679 515 Size: 62 730 1741 Fragments arranged by size: 1741 730 611 190 171 97 93 85 74 62 51 BspEI T'CCGG_A Cuts at: 246 704 3125 246 Size: 458 2421 1026 Fragments arranged by size: 2421 1026 458 BspHI T'CATG_A Cuts at: 3081 3081 Size: 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 233 667 782 955 1169 1471 1852 233 Size: 434 115 173 214 302 381 2286 Fragments arranged by size: 2286 434 381 302 214 173 115 BsrBI CCG'CTC Cuts at: 50 767 2197 2251 3735 3900 50 Size: 717 1430 54 1484 165 55 Fragments arranged by size: 1484 1430 717 165 55 54 BsrDI GCAATG_nn' Cuts at: 1817 1817 Size: 3905 BsrFI r'CCGG_y Cuts at: 429 1903 2084 2293 429 Size: 1474 181 209 2041 Fragments arranged by size: 2041 1474 209 181 BssSI C'ACGA_G Cuts at: 2176 2534 2176 Size: 358 3547 BstAPI GCAn_nnn'nTGC Cuts at: 581 1282 581 Size: 701 3204 BstBI TT'CG_AA Cuts at: 2266 2266 Size: 3905 BstXI CCAn_nnnn'nTGG Cuts at: 326 522 326 Size: 196 3709 BstYI r'GATC_y Cuts at: 1 251 546 930 1397 1420 1755 2001 3013 Size: 250 295 384 467 23 335 246 1012 Cuts at: 3013 3099 3111 3457 1 Size: 86 12 346 449 Fragments arranged by size: 1012 467 449 384 346 335 295 250 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1855 2036 2199 1855 Size: 181 163 3561 Fragments arranged by size: 3561 181 163 BtsI GCAGTG_nn' Cuts at: 741 1148 741 Size: 407 3498 DraI TTT'AAA Cuts at: 904 904 Size: 3905 DrdI GACnn_nn'nnGTC Cuts at: 378 1611 2469 378 Size: 1233 858 1814 Fragments arranged by size: 1814 1233 858 EaeI y'GGCC_r Cuts at: 1490 1664 2055 2082 3427 1490 Size: 174 391 27 1345 1968 Fragments arranged by size: 1968 1345 391 174 27 EagI C'GGCC_G Cuts at: 1490 1490 Size: 3905 EarI CTCTTCn'nnn_ Cuts at: 468 1928 2138 468 Size: 1460 210 2235 Fragments arranged by size: 2235 1460 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2113 2423 2569 3715 2113 Size: 310 146 1146 2303 Fragments arranged by size: 2303 1146 310 146 EcoICRI GAG'CTC Cuts at: 917 917 Size: 3905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 328 440 535 772 1730 2162 2909 3177 328 Size: 112 95 237 958 432 747 268 1056 Fragments arranged by size: 1056 958 747 432 268 237 112 95 EcoNI CCTnn'n_nnAGG Cuts at: 675 675 Size: 3905 EcoO109I rG'GnC_Cy Cuts at: 680 1044 680 Size: 364 3541 EcoRI G'AATT_C Cuts at: 909 909 Size: 3905 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1924 1924 Size: 3905 FspI TGC'GCA Cuts at: 1686 1686 Size: 3905 HaeII r_GCGC'y Cuts at: 307 1339 1587 2609 3588 307 Size: 1032 248 1022 979 624 Fragments arranged by size: 1032 1022 979 624 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1747 1777 1779 1809 1747 Size: 30 2 30 3843 Fragments arranged by size: 3843 30 30 2 HincII GTy'rAC Cuts at: 944 944 Size: 3905 KasI G'GCGC_C Cuts at: 303 1335 1583 303 Size: 1032 248 2625 Fragments arranged by size: 2625 1032 248 KpnI G_GTAC'C Cuts at: 925 925 Size: 3905 MfeI C'AATT_G Cuts at: 320 320 Size: 3905 MluI A'CGCG_T Cuts at: 3338 3338 Size: 3905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 164 794 2576 2760 164 Size: 630 1782 184 1309 Fragments arranged by size: 1782 1309 630 184 MscI TGG'CCA Cuts at: 1666 1666 Size: 3905 MslI CAynn'nnrTG Cuts at: 800 1894 2021 800 Size: 1094 127 2684 Fragments arranged by size: 2684 1094 127 MspA1I CmG'CkG Cuts at: 522 1016 1330 1690 2703 2948 522 Size: 494 314 360 1013 245 1479 Fragments arranged by size: 1479 1013 494 360 314 245 NaeI GCC'GGC Cuts at: 2086 2086 Size: 3905 NarI GG'CG_CC Cuts at: 304 1336 1584 304 Size: 1032 248 2625 Fragments arranged by size: 2625 1032 248 NgoMIV G'CCGG_C Cuts at: 2084 2084 Size: 3905 NspI r_CATG'y Cuts at: 958 1458 2365 958 Size: 500 907 2498 Fragments arranged by size: 2498 907 500 PasI CC'CwG_GG Cuts at: 356 356 Size: 3905 PciI A'CATG_T Cuts at: 2361 2361 Size: 3905 PflMI CCAn_nnn'nTGG Cuts at: 228 228 Size: 3905 PfoI T'CCnGG_A Cuts at: 344 344 Size: 3905 PmeI GTTT'AAAC Cuts at: 904 904 Size: 3905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3044 3076 3044 Size: 32 3873 PspOMI G'GGCC_C Cuts at: 779 779 Size: 3905 PstI C_TGCA'G Cuts at: 176 748 952 1637 176 Size: 572 204 685 2444 Fragments arranged by size: 2444 685 572 204 PvuI CG_AT'CG Cuts at: 104 104 Size: 3905 PvuII CAG'CTG Cuts at: 522 1330 1690 522 Size: 808 360 2737 Fragments arranged by size: 2737 808 360 RsrII CG'GwC_CG Cuts at: 2100 2100 Size: 3905 SacI G_AGCT'C Cuts at: 919 919 Size: 3905 SalI G'TCGA_C Cuts at: 942 942 Size: 3905 SapI GCTCTTCn'nnn_ Cuts at: 1928 2138 1928 Size: 210 3695 SbfI CC_TGCA'GG Cuts at: 952 952 Size: 3905 ScaI AGT'ACT Cuts at: 3422 3422 Size: 3905 SfcI C'TryA_G Cuts at: 33 162 172 744 948 1633 2626 2817 33 Size: 129 10 572 204 685 993 191 1121 Fragments arranged by size: 1121 993 685 572 204 191 129 10 SfoI GGC'GCC Cuts at: 305 1337 1585 305 Size: 1032 248 2625 Fragments arranged by size: 2625 1032 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3905 SmaI CCC'GGG Cuts at: 927 927 Size: 3905 SmlI C'TyrA_G Cuts at: 613 1060 1975 2467 2729 613 Size: 447 915 492 262 1789 Fragments arranged by size: 1789 915 492 447 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3905 SphI G_CATG'C Cuts at: 958 1458 958 Size: 500 3405 StuI AGG'CCT Cuts at: 481 565 670 481 Size: 84 105 3716 Fragments arranged by size: 3716 105 84 StyI C'CwwG_G Cuts at: 467 542 1039 1393 467 Size: 75 497 354 2979 Fragments arranged by size: 2979 497 354 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 595 1488 1621 2080 2289 595 Size: 893 133 459 209 2211 Fragments arranged by size: 2211 893 459 209 133 TatI w'GTAC_w Cuts at: 3420 3420 Size: 3905 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 859 891 3422 3454 859 Size: 32 2531 32 1310 Fragments arranged by size: 2531 1310 32 32 Tth111I GACn'n_nGTC Cuts at: 295 1702 295 Size: 1407 2498 XbaI T'CTAG_A Cuts at: 936 936 Size: 3905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 284 1400 284 Size: 1116 2789 XmaI C'CCGG_G Cuts at: 925 925 Size: 3905 XmnI GAAnn'nnTTC Cuts at: 670 727 670 Size: 57 3848 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI