Restriction map for
pF1KB7546
(Circular) (Six-base) MAPSORT of: pF1KB7546.seq Check: 2118 from: 1 to: 3905
pF1KB7546 3905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 233 233
Size: 3905
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 1730 2162 2909 3177 440
Size: 1290 432 747 268 1168
Fragments arranged by size:
1290 1168 747 432 268
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 1210 2777 1210
Size: 1567 2338
ApaI G_GGCC'C
Cuts at: 783 783
Size: 3905
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 909 3258 3514 909
Size: 2349 256 1300
Fragments arranged by size:
2349 1300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 925 925
Size: 3905
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 303 607 921 1335 1583 1618 303
Size: 304 314 414 248 35 2590
Fragments arranged by size:
2590 414 314 304 248 35
BanII G_rGCy'C
Cuts at: 783 919 1949 783
Size: 136 1030 2739
Fragments arranged by size:
2739 1030 136
BbeI G_GCGC'C
Cuts at: 307 1339 1587 307
Size: 1032 248 2625
Fragments arranged by size:
2625 1032 248
BbvCI CC'TCA_GC
Cuts at: 598 598
Size: 3905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 466 500 90
Size: 34 342 34 3495
Fragments arranged by size:
3495 342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 515 612 783 1530 1623 2679 515
Size: 97 171 747 93 1056 1741
Fragments arranged by size:
1741 1056 747 171 97 93
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1198 1533 3677 1117
Size: 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 390 2272 390
Size: 1882 2023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 535 772 328
Size: 207 237 3461
Fragments arranged by size:
3461 237 207
Bpu10I CC'TnA_GC
Cuts at: 598 885 598
Size: 287 3618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1081 1960 2452 2750 598
Size: 483 879 492 298 1753
Fragments arranged by size:
1753 879 492 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 622 1419 3456 6
Size: 616 797 2037 455
Fragments arranged by size:
2037 797 616 455
BsaHI Gr'CG_yC
Cuts at: 304 1336 1584 2286 3782 304
Size: 1032 248 702 1496 427
Fragments arranged by size:
1496 1032 702 427 248
BsaWI w'CCGG_w
Cuts at: 246 704 1094 1615 2293 2567 2714 3125 246
Size: 458 390 521 678 274 147 411 1026
Fragments arranged by size:
1026 678 521 458 411 390 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 438 438
Size: 3905
BseYI C'CCAG_C
Cuts at: 314 407 415 527 1330 2088 2665 3161 314
Size: 93 8 112 803 758 577 496 1058
Fragments arranged by size:
1058 803 758 577 496 112 93 8
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 834 919 1697 1887 2679 834
Size: 85 778 190 792 2060
Fragments arranged by size:
2060 792 778 190 85
Bsp1286I G_dGCh'C
Cuts at: 515 612 783 834 919 1530 1623 1697 1887
Size: 97 171 51 85 611 93 74 190
Cuts at: 1887 1949 2679 515
Size: 62 730 1741
Fragments arranged by size:
1741 730 611 190 171 97 93 85
74 62 51
BspEI T'CCGG_A
Cuts at: 246 704 3125 246
Size: 458 2421 1026
Fragments arranged by size:
2421 1026 458
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 667 782 955 1169 1471 1852 233
Size: 434 115 173 214 302 381 2286
Fragments arranged by size:
2286 434 381 302 214 173 115
BsrBI CCG'CTC
Cuts at: 50 767 2197 2251 3735 3900 50
Size: 717 1430 54 1484 165 55
Fragments arranged by size:
1484 1430 717 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 429 1903 2084 2293 429
Size: 1474 181 209 2041
Fragments arranged by size:
2041 1474 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 581 1282 581
Size: 701 3204
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstXI CCAn_nnnn'nTGG
Cuts at: 326 522 326
Size: 196 3709
BstYI r'GATC_y
Cuts at: 1 251 546 930 1397 1420 1755 2001 3013
Size: 250 295 384 467 23 335 246 1012
Cuts at: 3013 3099 3111 3457 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 384 346 335 295 250
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 741 1148 741
Size: 407 3498
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 378 1611 2469 378
Size: 1233 858 1814
Fragments arranged by size:
1814 1233 858
EaeI y'GGCC_r
Cuts at: 1490 1664 2055 2082 3427 1490
Size: 174 391 27 1345 1968
Fragments arranged by size:
1968 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 468 1928 2138 468
Size: 1460 210 2235
Fragments arranged by size:
2235 1460 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 440 535 772 1730 2162 2909 3177 328
Size: 112 95 237 958 432 747 268 1056
Fragments arranged by size:
1056 958 747 432 268 237 112 95
EcoNI CCTnn'n_nnAGG
Cuts at: 675 675
Size: 3905
EcoO109I rG'GnC_Cy
Cuts at: 680 1044 680
Size: 364 3541
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 307 1339 1587 2609 3588 307
Size: 1032 248 1022 979 624
Fragments arranged by size:
1032 1022 979 624 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1747 1777 1779 1809 1747
Size: 30 2 30 3843
Fragments arranged by size:
3843 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 303 1335 1583 303
Size: 1032 248 2625
Fragments arranged by size:
2625 1032 248
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MfeI C'AATT_G
Cuts at: 320 320
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 794 2576 2760 164
Size: 630 1782 184 1309
Fragments arranged by size:
1782 1309 630 184
MscI TGG'CCA
Cuts at: 1666 1666
Size: 3905
MslI CAynn'nnrTG
Cuts at: 800 1894 2021 800
Size: 1094 127 2684
Fragments arranged by size:
2684 1094 127
MspA1I CmG'CkG
Cuts at: 522 1016 1330 1690 2703 2948 522
Size: 494 314 360 1013 245 1479
Fragments arranged by size:
1479 1013 494 360 314 245
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 304 1336 1584 304
Size: 1032 248 2625
Fragments arranged by size:
2625 1032 248
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PasI CC'CwG_GG
Cuts at: 356 356
Size: 3905
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PflMI CCAn_nnn'nTGG
Cuts at: 228 228
Size: 3905
PfoI T'CCnGG_A
Cuts at: 344 344
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PspOMI G'GGCC_C
Cuts at: 779 779
Size: 3905
PstI C_TGCA'G
Cuts at: 176 748 952 1637 176
Size: 572 204 685 2444
Fragments arranged by size:
2444 685 572 204
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 522 1330 1690 522
Size: 808 360 2737
Fragments arranged by size:
2737 808 360
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 162 172 744 948 1633 2626 2817 33
Size: 129 10 572 204 685 993 191 1121
Fragments arranged by size:
1121 993 685 572 204 191 129 10
SfoI GGC'GCC
Cuts at: 305 1337 1585 305
Size: 1032 248 2625
Fragments arranged by size:
2625 1032 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 613 1060 1975 2467 2729 613
Size: 447 915 492 262 1789
Fragments arranged by size:
1789 915 492 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
StuI AGG'CCT
Cuts at: 481 565 670 481
Size: 84 105 3716
Fragments arranged by size:
3716 105 84
StyI C'CwwG_G
Cuts at: 467 542 1039 1393 467
Size: 75 497 354 2979
Fragments arranged by size:
2979 497 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 595 1488 1621 2080 2289 595
Size: 893 133 459 209 2211
Fragments arranged by size:
2211 893 459 209 133
TatI w'GTAC_w
Cuts at: 3420 3420
Size: 3905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 859 891 3422 3454 859
Size: 32 2531 32 1310
Fragments arranged by size:
2531 1310 32 32
Tth111I GACn'n_nGTC
Cuts at: 295 1702 295
Size: 1407 2498
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 284 1400 284
Size: 1116 2789
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
XmnI GAAnn'nnTTC
Cuts at: 670 727 670
Size: 57 3848
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI