Restriction map for pF1KB7546
 (Circular) (Six-base) MAPSORT of: pF1KB7546.seq  Check: 2118  from: 1  to: 3905



pF1KB7546  3905 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    233     233 
   Size:       3905
 
AccI GT'mk_AC

Cuts at:    943     943 
   Size:       3905
 
Acc65I G'GTAC_C

Cuts at:    921     921 
   Size:       3905
 
AclI AA'CG_TT

Cuts at:   3745    3745 
   Size:       3905
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    440    1730    2162    2909    3177     440 
   Size:       1290     432     747     268    1168

  Fragments arranged by size: 

                1290    1168     747     432     268
 
AflIII A'CryG_T

Cuts at:   1257    2361    3338    1257 
   Size:       1104     977    1824

  Fragments arranged by size: 

                1824    1104     977
 
AgeI A'CCGG_T

Cuts at:   2293    2293 
   Size:       3905
 
AlwNI CAG_nnn'CTG

Cuts at:   1210    2777    1210 
   Size:       1567    2338
 
ApaI G_GGCC'C

Cuts at:    783     783 
   Size:       3905
 
ApaLI G'TGCA_C

Cuts at:   2675    2675 
   Size:       3905
 
ApoI r'AATT_y

Cuts at:    909    3258    3514     909 
   Size:       2349     256    1300

  Fragments arranged by size: 

                2349    1300     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3905
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3905
 
AvaI C'yCGr_G

Cuts at:    925     925 
   Size:       3905
 
BamHI G'GATC_C

Cuts at:    930     930 
   Size:       3905
 
BanI G'GyrC_C

Cuts at:    303     607     921    1335    1583    1618     303 
   Size:        304     314     414     248      35    2590

  Fragments arranged by size: 

                2590     414     314     304     248      35
 
BanII G_rGCy'C

Cuts at:    783     919    1949     783 
   Size:        136    1030    2739

  Fragments arranged by size: 

                2739    1030     136
 
BbeI G_GCGC'C

Cuts at:    307    1339    1587     307 
   Size:       1032     248    2625

  Fragments arranged by size: 

                2625    1032     248
 
BbvCI CC'TCA_GC

Cuts at:    598     598 
   Size:       3905
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     466     500      90 
   Size:         34     342      34    3495

  Fragments arranged by size: 

                3495     342      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1803    2564    3905    1803 
   Size:        761    1341    1803

  Fragments arranged by size: 

                1803    1341     761
 
BclI T'GATC_A

Cuts at:   1425    1425 
   Size:       3905
 
BglII A'GATC_T

Cuts at:   1420    3457    1420 
   Size:       2037    1868
 
BlpI GC'TnA_GC

Cuts at:   1017    1017 
   Size:       3905
 
Bme1580I G_kGCm'C

Cuts at:    515     612     783    1530    1623    2679     515 
   Size:         97     171     747      93    1056    1741

  Fragments arranged by size: 

                1741    1056     747     171      97      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1117    1198    1533    3677    1117 
   Size:         81     335    2144    1345

  Fragments arranged by size: 

                2144    1345     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    390    2272     390 
   Size:       1882    2023
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    328     535     772     328 
   Size:        207     237    3461

  Fragments arranged by size: 

                3461     237     207
 
Bpu10I CC'TnA_GC

Cuts at:    598     885     598 
   Size:        287    3618
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    598    1081    1960    2452    2750     598 
   Size:        483     879     492     298    1753

  Fragments arranged by size: 

                1753     879     492     483     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3609    3609 
   Size:       3905
 
BsaAI yAC'GTr

Cuts at:   1258    1258 
   Size:       3905
 
BsaBI GATnn'nnATC

Cuts at:      6     622    1419    3456       6 
   Size:        616     797    2037     455

  Fragments arranged by size: 

                2037     797     616     455
 
BsaHI Gr'CG_yC

Cuts at:    304    1336    1584    2286    3782     304 
   Size:       1032     248     702    1496     427

  Fragments arranged by size: 

                1496    1032     702     427     248
 
BsaWI w'CCGG_w

Cuts at:    246     704    1094    1615    2293    2567    2714    3125     246 
   Size:        458     390     521     678     274     147     411    1026

  Fragments arranged by size: 

                1026     678     521     458     411     390     274     147
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    438     438 
   Size:       3905
 
BseYI C'CCAG_C

Cuts at:    314     407     415     527    1330    2088    2665    3161     314 
   Size:         93       8     112     803     758     577     496    1058

  Fragments arranged by size: 

                1058     803     758     577     496     112      93       8
            
 
BsiEI CG_ry'CG

Cuts at:    104    1447    1493    2701     104 
   Size:       1343      46    1208    1308

  Fragments arranged by size: 

                1343    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    834     919    1697    1887    2679     834 
   Size:         85     778     190     792    2060

  Fragments arranged by size: 

                2060     792     778     190      85
 
Bsp1286I G_dGCh'C

Cuts at:    515     612     783     834     919    1530    1623    1697    1887 
   Size:         97     171      51      85     611      93      74     190

Cuts at:   1887    1949    2679     515 
   Size:         62     730    1741

  Fragments arranged by size: 

                1741     730     611     190     171      97      93      85
                  74      62      51
 
BspEI T'CCGG_A

Cuts at:    246     704    3125     246 
   Size:        458    2421    1026

  Fragments arranged by size: 

                2421    1026     458
 
BspHI T'CATG_A

Cuts at:   3081    3081 
   Size:       3905
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    233     667     782     955    1169    1471    1852     233 
   Size:        434     115     173     214     302     381    2286

  Fragments arranged by size: 

                2286     434     381     302     214     173     115
 
BsrBI CCG'CTC

Cuts at:     50     767    2197    2251    3735    3900      50 
   Size:        717    1430      54    1484     165      55

  Fragments arranged by size: 

                1484    1430     717     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1817    1817 
   Size:       3905
 
BsrFI r'CCGG_y

Cuts at:    429    1903    2084    2293     429 
   Size:       1474     181     209    2041

  Fragments arranged by size: 

                2041    1474     209     181
 
BssSI C'ACGA_G

Cuts at:   2176    2534    2176 
   Size:        358    3547
 
BstAPI GCAn_nnn'nTGC

Cuts at:    581    1282     581 
   Size:        701    3204
 
BstBI TT'CG_AA

Cuts at:   2266    2266 
   Size:       3905
 
BstXI CCAn_nnnn'nTGG

Cuts at:    326     522     326 
   Size:        196    3709
 
BstYI r'GATC_y

Cuts at:      1     251     546     930    1397    1420    1755    2001    3013 
   Size:        250     295     384     467      23     335     246    1012

Cuts at:   3013    3099    3111    3457       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     384     346     335     295     250
                 246      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1855    2036    2199    1855 
   Size:        181     163    3561

  Fragments arranged by size: 

                3561     181     163
 
BtsI GCAGTG_nn'

Cuts at:    741    1148     741 
   Size:        407    3498
 
DraI TTT'AAA

Cuts at:    904     904 
   Size:       3905
 
DrdI GACnn_nn'nnGTC

Cuts at:    378    1611    2469     378 
   Size:       1233     858    1814

  Fragments arranged by size: 

                1814    1233     858
 
EaeI y'GGCC_r

Cuts at:   1490    1664    2055    2082    3427    1490 
   Size:        174     391      27    1345    1968

  Fragments arranged by size: 

                1968    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1490    1490 
   Size:       3905
 
EarI CTCTTCn'nnn_

Cuts at:    468    1928    2138     468 
   Size:       1460     210    2235

  Fragments arranged by size: 

                2235    1460     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2113    2423    2569    3715    2113 
   Size:        310     146    1146    2303

  Fragments arranged by size: 

                2303    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    917     917 
   Size:       3905
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    328     440     535     772    1730    2162    2909    3177     328 
   Size:        112      95     237     958     432     747     268    1056

  Fragments arranged by size: 

                1056     958     747     432     268     237     112      95
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    675     675 
   Size:       3905
 
EcoO109I rG'GnC_Cy

Cuts at:    680    1044     680 
   Size:        364    3541
 
EcoRI G'AATT_C

Cuts at:    909     909 
   Size:       3905
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1924    1924 
   Size:       3905
 
FspI TGC'GCA

Cuts at:   1686    1686 
   Size:       3905
 
HaeII r_GCGC'y

Cuts at:    307    1339    1587    2609    3588     307 
   Size:       1032     248    1022     979     624

  Fragments arranged by size: 

                1032    1022     979     624     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1747    1777    1779    1809    1747 
   Size:         30       2      30    3843

  Fragments arranged by size: 

                3843      30      30       2
 
HincII GTy'rAC

Cuts at:    944     944 
   Size:       3905
 
KasI G'GCGC_C

Cuts at:    303    1335    1583     303 
   Size:       1032     248    2625

  Fragments arranged by size: 

                2625    1032     248
 
KpnI G_GTAC'C

Cuts at:    925     925 
   Size:       3905
 
MfeI C'AATT_G

Cuts at:    320     320 
   Size:       3905
 
MluI A'CGCG_T

Cuts at:   3338    3338 
   Size:       3905
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    164     794    2576    2760     164 
   Size:        630    1782     184    1309

  Fragments arranged by size: 

                1782    1309     630     184
 
MscI TGG'CCA

Cuts at:   1666    1666 
   Size:       3905
 
MslI CAynn'nnrTG

Cuts at:    800    1894    2021     800 
   Size:       1094     127    2684

  Fragments arranged by size: 

                2684    1094     127
 
MspA1I CmG'CkG

Cuts at:    522    1016    1330    1690    2703    2948     522 
   Size:        494     314     360    1013     245    1479

  Fragments arranged by size: 

                1479    1013     494     360     314     245
 
NaeI GCC'GGC

Cuts at:   2086    2086 
   Size:       3905
 
NarI GG'CG_CC

Cuts at:    304    1336    1584     304 
   Size:       1032     248    2625

  Fragments arranged by size: 

                2625    1032     248
 
NgoMIV G'CCGG_C

Cuts at:   2084    2084 
   Size:       3905
 
NspI r_CATG'y

Cuts at:    958    1458    2365     958 
   Size:        500     907    2498

  Fragments arranged by size: 

                2498     907     500
 
PasI CC'CwG_GG

Cuts at:    356     356 
   Size:       3905
 
PciI A'CATG_T

Cuts at:   2361    2361 
   Size:       3905
 
PflMI CCAn_nnn'nTGG

Cuts at:    228     228 
   Size:       3905
 
PfoI T'CCnGG_A

Cuts at:    344     344 
   Size:       3905
 
PmeI GTTT'AAAC

Cuts at:    904     904 
   Size:       3905
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3044    3076    3044 
   Size:         32    3873
 
PspOMI G'GGCC_C

Cuts at:    779     779 
   Size:       3905
 
PstI C_TGCA'G

Cuts at:    176     748     952    1637     176 
   Size:        572     204     685    2444

  Fragments arranged by size: 

                2444     685     572     204
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3905
 
PvuII CAG'CTG

Cuts at:    522    1330    1690     522 
   Size:        808     360    2737

  Fragments arranged by size: 

                2737     808     360
 
RsrII CG'GwC_CG

Cuts at:   2100    2100 
   Size:       3905
 
SacI G_AGCT'C

Cuts at:    919     919 
   Size:       3905
 
SalI G'TCGA_C

Cuts at:    942     942 
   Size:       3905
 
SapI GCTCTTCn'nnn_

Cuts at:   1928    2138    1928 
   Size:        210    3695
 
SbfI CC_TGCA'GG

Cuts at:    952     952 
   Size:       3905
 
ScaI AGT'ACT

Cuts at:   3422    3422 
   Size:       3905
 
SfcI C'TryA_G

Cuts at:     33     162     172     744     948    1633    2626    2817      33 
   Size:        129      10     572     204     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     572     204     191     129      10
            
 
SfoI GGC'GCC

Cuts at:    305    1337    1585     305 
   Size:       1032     248    2625

  Fragments arranged by size: 

                2625    1032     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3905
 
SmaI CCC'GGG

Cuts at:    927     927 
   Size:       3905
 
SmlI C'TyrA_G

Cuts at:    613    1060    1975    2467    2729     613 
   Size:        447     915     492     262    1789

  Fragments arranged by size: 

                1789     915     492     447     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3905
 
SphI G_CATG'C

Cuts at:    958    1458     958 
   Size:        500    3405
 
StuI AGG'CCT

Cuts at:    481     565     670     481 
   Size:         84     105    3716

  Fragments arranged by size: 

                3716     105      84
 
StyI C'CwwG_G

Cuts at:    467     542    1039    1393     467 
   Size:         75     497     354    2979

  Fragments arranged by size: 

                2979     497     354      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    595    1488    1621    2080    2289     595 
   Size:        893     133     459     209    2211

  Fragments arranged by size: 

                2211     893     459     209     133
 
TatI w'GTAC_w

Cuts at:   3420    3420 
   Size:       3905
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    859     891    3422    3454     859 
   Size:         32    2531      32    1310

  Fragments arranged by size: 

                2531    1310      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    295    1702     295 
   Size:       1407    2498
 
XbaI T'CTAG_A

Cuts at:    936     936 
   Size:       3905
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    284    1400     284 
   Size:       1116    2789
 
XmaI C'CCGG_G

Cuts at:    925     925 
   Size:       3905
 
XmnI GAAnn'nnTTC

Cuts at:    670     727     670 
   Size:         57    3848
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BfrBI     BglI    BmgBI     BmtI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI
  HindIII     HpaI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     ZraI
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