Restriction map for
pF1KB7547
(Circular) (Six-base) MAPSORT of: pF1KB7547.seq Check: 6106 from: 1 to: 3668
pF1KB7547 3668 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 259 259
Size: 3668
AccI GT'mk_AC
Cuts at: 285 706 285
Size: 421 3247
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 1493 1925 2672 2940 106
Size: 1387 432 747 268 834
Fragments arranged by size:
1387 834 747 432 268
AflIII A'CryG_T
Cuts at: 288 1020 2124 3101 288
Size: 732 1104 977 855
Fragments arranged by size:
1104 977 855 732
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 267 267
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 239 973 2540 239
Size: 734 1567 1367
Fragments arranged by size:
1567 1367 734
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 639 672 3021 3277 639
Size: 33 2349 256 1030
Fragments arranged by size:
2349 1030 256 33
AseI AT'TA_AT
Cuts at: 20 220 20
Size: 200 3468
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 139 688 139
Size: 549 3119
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 684 1098 1346 1381 684
Size: 414 248 35 2971
Fragments arranged by size:
2971 414 248 35
BanII G_rGCy'C
Cuts at: 682 1712 682
Size: 1030 2638
BbeI G_GCGC'C
Cuts at: 1102 1350 1102
Size: 248 3420
BbsI GAAGACnn'nnnn_
Cuts at: 441 530 560 441
Size: 89 30 3549
Fragments arranged by size:
3549 89 30
BciVI GTATCCnnnnn_n'
Cuts at: 1566 2327 3668 1566
Size: 761 1341 1566
Fragments arranged by size:
1566 1341 761
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BglII A'GATC_T
Cuts at: 1183 3220 1183
Size: 2037 1631
BlpI GC'TnA_GC
Cuts at: 134 780 134
Size: 646 3022
Bme1580I G_kGCm'C
Cuts at: 1293 1386 2442 1293
Size: 93 1056 2519
Fragments arranged by size:
2519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 299 880 961 1296 3440 299
Size: 581 81 335 2144 527
Fragments arranged by size:
2144 581 527 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1723 2215 2513 844
Size: 879 492 298 1999
Fragments arranged by size:
1999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3372 3372
Size: 3668
BsaAI yAC'GTr
Cuts at: 1021 1021
Size: 3668
BsaBI GATnn'nnATC
Cuts at: 6 1182 3219 6
Size: 1176 2037 455
Fragments arranged by size:
2037 1176 455
BsaHI Gr'CG_yC
Cuts at: 1099 1347 2049 3545 1099
Size: 248 702 1496 1222
Fragments arranged by size:
1496 1222 702 248
BsaWI w'CCGG_w
Cuts at: 857 1378 2056 2330 2477 2888 857
Size: 521 678 274 147 411 1637
Fragments arranged by size:
1637 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 115 1093 1851 2428 2924 115
Size: 978 758 577 496 859
Fragments arranged by size:
978 859 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1210 1256 2464 104
Size: 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 682 1460 1650 2442 682
Size: 778 190 792 1908
Fragments arranged by size:
1908 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 682 1293 1386 1460 1650 1712 2442 682
Size: 611 93 74 190 62 730 1908
Fragments arranged by size:
1908 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 3668
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 259 718 932 1234 1615 259
Size: 459 214 302 381 2312
Fragments arranged by size:
2312 459 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1580 1580
Size: 3668
BsrFI r'CCGG_y
Cuts at: 1666 1847 2056 1666
Size: 181 209 3278
Fragments arranged by size:
3278 209 181
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 1045 1045
Size: 3668
BstBI TT'CG_AA
Cuts at: 361 2029 361
Size: 1668 2000
BstYI r'GATC_y
Cuts at: 1 693 1160 1183 1518 1764 2776 2862 2874
Size: 692 467 23 335 246 1012 86 12
Cuts at: 2874 3220 1
Size: 346 449
Fragments arranged by size:
1012 692 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 911 911
Size: 3668
ClaI AT'CG_AT
Cuts at: 512 512
Size: 3668
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2232 1374
Size: 858 2810
EaeI y'GGCC_r
Cuts at: 1253 1427 1818 1845 3190 1253
Size: 174 391 27 1345 1731
Fragments arranged by size:
1731 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 680 680
Size: 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 1493 1925 2672 2940 106
Size: 1387 432 747 268 834
Fragments arranged by size:
1387 834 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 236 602 807 236
Size: 366 205 3097
Fragments arranged by size:
3097 366 205
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 416 464 1687 416
Size: 48 1223 2397
Fragments arranged by size:
2397 1223 48
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 134 1102 1350 2372 3351 134
Size: 968 248 1022 979 451
Fragments arranged by size:
1022 979 968 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1510 1540 1542 1572 1510
Size: 30 2 30 3606
Fragments arranged by size:
3606 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 1098 1346 1098
Size: 248 3420
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MfeI C'AATT_G
Cuts at: 173 173
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2523 2339
Size: 184 3484
MscI TGG'CCA
Cuts at: 1429 1429
Size: 3668
MslI CAynn'nnrTG
Cuts at: 1657 1784 1657
Size: 127 3541
MspA1I CmG'CkG
Cuts at: 779 1093 1453 2466 2711 779
Size: 314 360 1013 245 1736
Fragments arranged by size:
1736 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1849 1849
Size: 3668
NarI GG'CG_CC
Cuts at: 1099 1347 1099
Size: 248 3420
NgoMIV G'CCGG_C
Cuts at: 1847 1847
Size: 3668
NspI r_CATG'y
Cuts at: 292 721 1221 2128 292
Size: 429 500 907 1832
Fragments arranged by size:
1832 907 500 429
PasI CC'CwG_GG
Cuts at: 239 239
Size: 3668
PciI A'CATG_T
Cuts at: 288 2124 288
Size: 1836 1832
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PpuMI rG'GwC_Cy
Cuts at: 602 602
Size: 3668
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 435 467 435
Size: 32 3636
PstI C_TGCA'G
Cuts at: 256 715 1400 256
Size: 459 685 2524
Fragments arranged by size:
2524 685 459
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3668
PvuII CAG'CTG
Cuts at: 1093 1453 1093
Size: 360 3308
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 682 682
Size: 3668
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 252 711 1396 2389 2580 33
Size: 219 459 685 993 191 1121
Fragments arranged by size:
1121 993 685 459 219 191
SfoI GGC'GCC
Cuts at: 1100 1348 1100
Size: 248 3420
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 690 690
Size: 3668
SmlI C'TyrA_G
Cuts at: 823 1738 2230 2492 823
Size: 915 492 262 1999
Fragments arranged by size:
1999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StyI C'CwwG_G
Cuts at: 802 1156 802
Size: 354 3314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 353 1251 1384 1843 2052 353
Size: 898 133 459 209 1969
Fragments arranged by size:
1969 898 459 209 133
TatI w'GTAC_w
Cuts at: 3183 3183
Size: 3668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 3636
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 546 699 546
Size: 153 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1163
Size: 3668
XmaI C'CCGG_G
Cuts at: 688 688
Size: 3668
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI