Restriction map for
pF1KB7549
(Circular) (Six-base) MAPSORT of: pF1KB7549.seq Check: 5620 from: 1 to: 3692
pF1KB7549 3692 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 730 730
Size: 3692
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1517 1949 2696 2964 376
Size: 1141 432 747 268 1104
Fragments arranged by size:
1141 1104 747 432 268
AflIII A'CryG_T
Cuts at: 119 1044 2148 3125 119
Size: 925 1104 977 686
Fragments arranged by size:
1104 977 925 686
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AhdI GACnn_n'nnGTC
Cuts at: 234 234
Size: 3692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 187 593 187
Size: 406 3286
AlwNI CAG_nnn'CTG
Cuts at: 240 997 2564 240
Size: 757 1567 1368
Fragments arranged by size:
1567 1368 757
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 696 3045 3301 696
Size: 2349 256 1087
Fragments arranged by size:
2349 1087 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 443 712 443
Size: 269 3423
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 541 708 1122 1370 1405 541
Size: 167 414 248 35 2828
Fragments arranged by size:
2828 414 248 167 35
BanII G_rGCy'C
Cuts at: 706 1736 706
Size: 1030 2662
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BbsI GAAGACnn'nnnn_
Cuts at: 313 313
Size: 3692
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglI GCCn_nnn'nGGC
Cuts at: 477 477
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 1317 1410 2466 1317
Size: 93 1056 2543
Fragments arranged by size:
2543 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 658 904 985 1320 3464 658
Size: 246 81 335 2144 886
Fragments arranged by size:
2144 886 335 246 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1747 2239 2537 868
Size: 879 492 298 2023
Fragments arranged by size:
2023 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 550 3396 550
Size: 2846 846
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 881 1402 2080 2354 2501 2912 881
Size: 521 678 274 147 411 1661
Fragments arranged by size:
1661 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 644 674 644
Size: 30 3662
BseYI C'CCAG_C
Cuts at: 312 1117 1875 2452 2948 312
Size: 805 758 577 496 1056
Fragments arranged by size:
1056 805 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 447 589 400
Size: 47 142 3503
Fragments arranged by size:
3503 142 47
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 439 706 1484 1674 2466 439
Size: 267 778 190 792 1665
Fragments arranged by size:
1665 792 778 267 190
Bsp1286I G_dGCh'C
Cuts at: 439 706 1317 1410 1484 1674 1736 2466 439
Size: 267 611 93 74 190 62 730 1665
Fragments arranged by size:
1665 730 611 267 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2912 2912
Size: 3692
BspHI T'CATG_A
Cuts at: 338 2868 338
Size: 2530 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 372 742 956 1258 1639 372
Size: 370 214 302 381 2425
Fragments arranged by size:
2425 381 370 302 214
BsrBI CCG'CTC
Cuts at: 50 131 1984 2038 3522 3687 50
Size: 81 1853 54 1484 165 55
Fragments arranged by size:
1853 1484 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 639 1690 1871 2080 639
Size: 1051 181 209 2251
Fragments arranged by size:
2251 1051 209 181
BssSI C'ACGA_G
Cuts at: 1963 2321 1963
Size: 358 3334
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 374 2053 374
Size: 1679 2013
BstXI CCAn_nnnn'nTGG
Cuts at: 480 480
Size: 3692
BstYI r'GATC_y
Cuts at: 1 572 717 1184 1207 1542 1788 2800 2886
Size: 571 145 467 23 335 246 1012 86
Cuts at: 2886 2898 3244 1
Size: 12 346 449
Fragments arranged by size:
1012 571 467 449 346 335 246 145
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 935 935
Size: 3692
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2256 1398
Size: 858 2834
EaeI y'GGCC_r
Cuts at: 201 1277 1451 1842 1869 3214 201
Size: 1076 174 391 27 1345 679
Fragments arranged by size:
1345 1076 679 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1517 1949 2696 2964 376
Size: 1141 432 747 268 1104
Fragments arranged by size:
1141 1104 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 831 831
Size: 3692
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 1126 1374 2396 3375 1126
Size: 248 1022 979 1443
Fragments arranged by size:
1443 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 557 589 1534 1564 1566 1596 220
Size: 32 305 32 945 30 2 30 2316
Fragments arranged by size:
2316 945 305 32 32 30 30 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 618 2363 2547 618
Size: 1745 184 1763
Fragments arranged by size:
1763 1745 184
MscI TGG'CCA
Cuts at: 203 1453 203
Size: 1250 2442
MslI CAynn'nnrTG
Cuts at: 472 1681 1808 472
Size: 1209 127 2356
Fragments arranged by size:
2356 1209 127
MspA1I CmG'CkG
Cuts at: 803 1117 1477 2490 2735 803
Size: 314 360 1013 245 1760
Fragments arranged by size:
1760 1013 360 314 245
NaeI GCC'GGC
Cuts at: 641 1873 641
Size: 1232 2460
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NgoMIV G'CCGG_C
Cuts at: 639 1871 639
Size: 1232 2460
NspI r_CATG'y
Cuts at: 290 745 1245 2152 290
Size: 455 500 907 1830
Fragments arranged by size:
1830 907 500 455
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PflMI CCAn_nnn'nTGG
Cuts at: 240 240
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PstI C_TGCA'G
Cuts at: 297 739 1424 297
Size: 442 685 2565
Fragments arranged by size:
2565 685 442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 1117 1477 1117
Size: 360 3332
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 150 293 735 1420 2413 2604 33
Size: 117 143 442 685 993 191 1121
Fragments arranged by size:
1121 993 685 442 191 143 117
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 445 714 445
Size: 269 3423
SmlI C'TyrA_G
Cuts at: 847 1762 2254 2516 847
Size: 915 492 262 2023
Fragments arranged by size:
2023 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StyI C'CwwG_G
Cuts at: 826 1180 826
Size: 354 3338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 1408 1867 2076 1275
Size: 133 459 209 2891
Fragments arranged by size:
2891 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 309 1187 309
Size: 878 2814
XmaI C'CCGG_G
Cuts at: 443 712 443
Size: 269 3423
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI