(Circular) (Six-base) MAPSORT of: pF1KB7549.seq Check: 5620 from: 1 to: 3692 pF1KB7549 3692 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 730 730 Size: 3692 Acc65I G'GTAC_C Cuts at: 708 708 Size: 3692 AclI AA'CG_TT Cuts at: 3532 3532 Size: 3692 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 376 1517 1949 2696 2964 376 Size: 1141 432 747 268 1104 Fragments arranged by size: 1141 1104 747 432 268 AflIII A'CryG_T Cuts at: 119 1044 2148 3125 119 Size: 925 1104 977 686 Fragments arranged by size: 1104 977 925 686 AgeI A'CCGG_T Cuts at: 2080 2080 Size: 3692 AhdI GACnn_n'nnGTC Cuts at: 234 234 Size: 3692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 187 593 187 Size: 406 3286 AlwNI CAG_nnn'CTG Cuts at: 240 997 2564 240 Size: 757 1567 1368 Fragments arranged by size: 1567 1368 757 ApaLI G'TGCA_C Cuts at: 2462 2462 Size: 3692 ApoI r'AATT_y Cuts at: 696 3045 3301 696 Size: 2349 256 1087 Fragments arranged by size: 2349 1087 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3692 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3692 AvaI C'yCGr_G Cuts at: 443 712 443 Size: 269 3423 BamHI G'GATC_C Cuts at: 717 717 Size: 3692 BanI G'GyrC_C Cuts at: 541 708 1122 1370 1405 541 Size: 167 414 248 35 2828 Fragments arranged by size: 2828 414 248 167 35 BanII G_rGCy'C Cuts at: 706 1736 706 Size: 1030 2662 BbeI G_GCGC'C Cuts at: 1126 1374 1126 Size: 248 3444 BbsI GAAGACnn'nnnn_ Cuts at: 313 313 Size: 3692 BciVI GTATCCnnnnn_n' Cuts at: 1590 2351 3692 1590 Size: 761 1341 1590 Fragments arranged by size: 1590 1341 761 BclI T'GATC_A Cuts at: 1212 1212 Size: 3692 BglI GCCn_nnn'nGGC Cuts at: 477 477 Size: 3692 BglII A'GATC_T Cuts at: 1207 3244 1207 Size: 2037 1655 BlpI GC'TnA_GC Cuts at: 804 804 Size: 3692 Bme1580I G_kGCm'C Cuts at: 1317 1410 2466 1317 Size: 93 1056 2543 Fragments arranged by size: 2543 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 658 904 985 1320 3464 658 Size: 246 81 335 2144 886 Fragments arranged by size: 2144 886 335 246 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2059 2059 Size: 3692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 868 1747 2239 2537 868 Size: 879 492 298 2023 Fragments arranged by size: 2023 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 550 3396 550 Size: 2846 846 BsaAI yAC'GTr Cuts at: 1045 1045 Size: 3692 BsaBI GATnn'nnATC Cuts at: 6 1206 3243 6 Size: 1200 2037 455 Fragments arranged by size: 2037 1200 455 BsaHI Gr'CG_yC Cuts at: 1123 1371 2073 3569 1123 Size: 248 702 1496 1246 Fragments arranged by size: 1496 1246 702 248 BsaWI w'CCGG_w Cuts at: 881 1402 2080 2354 2501 2912 881 Size: 521 678 274 147 411 1661 Fragments arranged by size: 1661 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 644 674 644 Size: 30 3662 BseYI C'CCAG_C Cuts at: 312 1117 1875 2452 2948 312 Size: 805 758 577 496 1056 Fragments arranged by size: 1056 805 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 400 447 589 400 Size: 47 142 3503 Fragments arranged by size: 3503 142 47 BsiEI CG_ry'CG Cuts at: 104 1234 1280 2488 104 Size: 1130 46 1208 1308 Fragments arranged by size: 1308 1208 1130 46 BsiHKAI G_wGCw'C Cuts at: 439 706 1484 1674 2466 439 Size: 267 778 190 792 1665 Fragments arranged by size: 1665 792 778 267 190 Bsp1286I G_dGCh'C Cuts at: 439 706 1317 1410 1484 1674 1736 2466 439 Size: 267 611 93 74 190 62 730 1665 Fragments arranged by size: 1665 730 611 267 190 93 74 62 BspEI T'CCGG_A Cuts at: 2912 2912 Size: 3692 BspHI T'CATG_A Cuts at: 338 2868 338 Size: 2530 1162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 372 742 956 1258 1639 372 Size: 370 214 302 381 2425 Fragments arranged by size: 2425 381 370 302 214 BsrBI CCG'CTC Cuts at: 50 131 1984 2038 3522 3687 50 Size: 81 1853 54 1484 165 55 Fragments arranged by size: 1853 1484 165 81 55 54 BsrDI GCAATG_nn' Cuts at: 1604 1604 Size: 3692 BsrFI r'CCGG_y Cuts at: 639 1690 1871 2080 639 Size: 1051 181 209 2251 Fragments arranged by size: 2251 1051 209 181 BssSI C'ACGA_G Cuts at: 1963 2321 1963 Size: 358 3334 BstAPI GCAn_nnn'nTGC Cuts at: 1069 1069 Size: 3692 BstBI TT'CG_AA Cuts at: 374 2053 374 Size: 1679 2013 BstXI CCAn_nnnn'nTGG Cuts at: 480 480 Size: 3692 BstYI r'GATC_y Cuts at: 1 572 717 1184 1207 1542 1788 2800 2886 Size: 571 145 467 23 335 246 1012 86 Cuts at: 2886 2898 3244 1 Size: 12 346 449 Fragments arranged by size: 1012 571 467 449 346 335 246 145 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1642 1823 1986 1642 Size: 181 163 3348 Fragments arranged by size: 3348 181 163 BtsI GCAGTG_nn' Cuts at: 935 935 Size: 3692 DraI TTT'AAA Cuts at: 691 691 Size: 3692 DrdI GACnn_nn'nnGTC Cuts at: 1398 2256 1398 Size: 858 2834 EaeI y'GGCC_r Cuts at: 201 1277 1451 1842 1869 3214 201 Size: 1076 174 391 27 1345 679 Fragments arranged by size: 1345 1076 679 391 174 27 EagI C'GGCC_G Cuts at: 1277 1277 Size: 3692 EarI CTCTTCn'nnn_ Cuts at: 1715 1925 1715 Size: 210 3482 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1900 2210 2356 3502 1900 Size: 310 146 1146 2090 Fragments arranged by size: 2090 1146 310 146 EcoICRI GAG'CTC Cuts at: 704 704 Size: 3692 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 376 1517 1949 2696 2964 376 Size: 1141 432 747 268 1104 Fragments arranged by size: 1141 1104 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 831 831 Size: 3692 EcoRI G'AATT_C Cuts at: 696 696 Size: 3692 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1711 1711 Size: 3692 FspI TGC'GCA Cuts at: 1473 1473 Size: 3692 HaeII r_GCGC'y Cuts at: 1126 1374 2396 3375 1126 Size: 248 1022 979 1443 Fragments arranged by size: 1443 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 220 252 557 589 1534 1564 1566 1596 220 Size: 32 305 32 945 30 2 30 2316 Fragments arranged by size: 2316 945 305 32 32 30 30 2 HincII GTy'rAC Cuts at: 731 731 Size: 3692 KasI G'GCGC_C Cuts at: 1122 1370 1122 Size: 248 3444 KpnI G_GTAC'C Cuts at: 712 712 Size: 3692 MluI A'CGCG_T Cuts at: 3125 3125 Size: 3692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 618 2363 2547 618 Size: 1745 184 1763 Fragments arranged by size: 1763 1745 184 MscI TGG'CCA Cuts at: 203 1453 203 Size: 1250 2442 MslI CAynn'nnrTG Cuts at: 472 1681 1808 472 Size: 1209 127 2356 Fragments arranged by size: 2356 1209 127 MspA1I CmG'CkG Cuts at: 803 1117 1477 2490 2735 803 Size: 314 360 1013 245 1760 Fragments arranged by size: 1760 1013 360 314 245 NaeI GCC'GGC Cuts at: 641 1873 641 Size: 1232 2460 NarI GG'CG_CC Cuts at: 1123 1371 1123 Size: 248 3444 NgoMIV G'CCGG_C Cuts at: 639 1871 639 Size: 1232 2460 NspI r_CATG'y Cuts at: 290 745 1245 2152 290 Size: 455 500 907 1830 Fragments arranged by size: 1830 907 500 455 PciI A'CATG_T Cuts at: 2148 2148 Size: 3692 PflMI CCAn_nnn'nTGG Cuts at: 240 240 Size: 3692 PmeI GTTT'AAAC Cuts at: 691 691 Size: 3692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2831 2863 2831 Size: 32 3660 PstI C_TGCA'G Cuts at: 297 739 1424 297 Size: 442 685 2565 Fragments arranged by size: 2565 685 442 PvuI CG_AT'CG Cuts at: 104 104 Size: 3692 PvuII CAG'CTG Cuts at: 1117 1477 1117 Size: 360 3332 RsrII CG'GwC_CG Cuts at: 1887 1887 Size: 3692 SacI G_AGCT'C Cuts at: 706 706 Size: 3692 SalI G'TCGA_C Cuts at: 729 729 Size: 3692 SapI GCTCTTCn'nnn_ Cuts at: 1715 1925 1715 Size: 210 3482 SbfI CC_TGCA'GG Cuts at: 739 739 Size: 3692 ScaI AGT'ACT Cuts at: 3209 3209 Size: 3692 SfcI C'TryA_G Cuts at: 33 150 293 735 1420 2413 2604 33 Size: 117 143 442 685 993 191 1121 Fragments arranged by size: 1121 993 685 442 191 143 117 SfoI GGC'GCC Cuts at: 1124 1372 1124 Size: 248 3444 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3692 SmaI CCC'GGG Cuts at: 445 714 445 Size: 269 3423 SmlI C'TyrA_G Cuts at: 847 1762 2254 2516 847 Size: 915 492 262 2023 Fragments arranged by size: 2023 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3692 SphI G_CATG'C Cuts at: 745 1245 745 Size: 500 3192 StyI C'CwwG_G Cuts at: 826 1180 826 Size: 354 3338 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1275 1408 1867 2076 1275 Size: 133 459 209 2891 Fragments arranged by size: 2891 459 209 133 TatI w'GTAC_w Cuts at: 3207 3207 Size: 3692 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3209 3241 3209 Size: 32 3660 Tth111I GACn'n_nGTC Cuts at: 1489 1489 Size: 3692 XbaI T'CTAG_A Cuts at: 723 723 Size: 3692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 309 1187 309 Size: 878 2814 XmaI C'CCGG_G Cuts at: 443 712 443 Size: 269 3423 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI