Restriction map for
pF1KB7550
(Circular) (Six-base) MAPSORT of: pF1KB7550.seq Check: 1599 from: 1 to: 3914
pF1KB7550 3914 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 472 472
Size: 3914
AatII G_ACGT'C
Cuts at: 536 536
Size: 3914
AccI GT'mk_AC
Cuts at: 952 952
Size: 3914
Acc65I G'GTAC_C
Cuts at: 930 930
Size: 3914
AclI AA'CG_TT
Cuts at: 3754 3754
Size: 3914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 823 1739 2171 2918 3186 380
Size: 443 916 432 747 268 1108
Fragments arranged by size:
1108 916 747 443 432 268
AfeI AGC'GCT
Cuts at: 664 664
Size: 3914
AflIII A'CryG_T
Cuts at: 597 716 746 1266 2370 3347 597
Size: 119 30 520 1104 977 1164
Fragments arranged by size:
1164 1104 977 520 119 30
AgeI A'CCGG_T
Cuts at: 2302 2302
Size: 3914
AleI CACnn'nnGTG
Cuts at: 616 616
Size: 3914
AlwNI CAG_nnn'CTG
Cuts at: 1219 2786 1219
Size: 1567 2347
ApaI G_GGCC'C
Cuts at: 335 335
Size: 3914
ApaLI G'TGCA_C
Cuts at: 2684 2684
Size: 3914
ApoI r'AATT_y
Cuts at: 918 3267 3523 918
Size: 2349 256 1309
Fragments arranged by size:
2349 1309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3914
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
AvaI C'yCGr_G
Cuts at: 328 934 328
Size: 606 3308
BamHI G'GATC_C
Cuts at: 939 939
Size: 3914
BanI G'GyrC_C
Cuts at: 542 930 1344 1592 1627 542
Size: 388 414 248 35 2829
Fragments arranged by size:
2829 414 388 248 35
BanII G_rGCy'C
Cuts at: 173 335 928 1958 173
Size: 162 593 1030 2129
Fragments arranged by size:
2129 1030 593 162
BbeI G_GCGC'C
Cuts at: 1348 1596 1348
Size: 248 3666
BbsI GAAGACnn'nnnn_
Cuts at: 368 368
Size: 3914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3880
BciVI GTATCCnnnnn_n'
Cuts at: 1812 2573 3914 1812
Size: 761 1341 1812
Fragments arranged by size:
1812 1341 761
BclI T'GATC_A
Cuts at: 1434 1434
Size: 3914
BglI GCCn_nnn'nGGC
Cuts at: 785 785
Size: 3914
BglII A'GATC_T
Cuts at: 194 1429 3466 194
Size: 1235 2037 642
Fragments arranged by size:
2037 1235 642
BlpI GC'TnA_GC
Cuts at: 1026 1026
Size: 3914
Bme1580I G_kGCm'C
Cuts at: 335 1539 1632 2688 335
Size: 1204 93 1056 1561
Fragments arranged by size:
1561 1204 1056 93
BmgBI CAC'GTC
Cuts at: 600 792 600
Size: 192 3722
BmrI ACTGGGnnnn_n'
Cuts at: 440 1126 1207 1542 3686 440
Size: 686 81 335 2144 668
Fragments arranged by size:
2144 686 668 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 3914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 694 183
Size: 511 3403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 670 1090 1969 2461 2759 574
Size: 96 420 879 492 298 1729
Fragments arranged by size:
1729 879 492 420 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 389 446 3618 389
Size: 57 3172 685
Fragments arranged by size:
3172 685 57
BsaAI yAC'GTr
Cuts at: 149 201 554 747 1267 149
Size: 52 353 193 520 2796
Fragments arranged by size:
2796 520 353 193 52
BsaBI GATnn'nnATC
Cuts at: 6 1428 3465 6
Size: 1422 2037 455
Fragments arranged by size:
2037 1422 455
BsaHI Gr'CG_yC
Cuts at: 533 1345 1593 2295 3791 533
Size: 812 248 702 1496 656
Fragments arranged by size:
1496 812 702 656 248
BsaWI w'CCGG_w
Cuts at: 1103 1624 2302 2576 2723 3134 1103
Size: 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 769 196
Size: 573 3341
BseYI C'CCAG_C
Cuts at: 347 1339 2097 2674 3170 347
Size: 992 758 577 496 1091
Fragments arranged by size:
1091 992 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 534 654 724 750 781 534
Size: 120 70 26 31 3667
Fragments arranged by size:
3667 120 70 31 26
BsiEI CG_ry'CG
Cuts at: 104 1456 1502 2710 104
Size: 1352 46 1208 1308
Fragments arranged by size:
1352 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 173 812 928 1706 1896 2688 173
Size: 639 116 778 190 792 1399
Fragments arranged by size:
1399 792 778 639 190 116
BsmBI CGTCTCn'nnnn_
Cuts at: 147 147
Size: 3914
Bsp1286I G_dGCh'C
Cuts at: 173 335 812 928 1539 1632 1706 1896 1958
Size: 162 477 116 611 93 74 190 62
Cuts at: 1958 2688 173
Size: 730 1399
Fragments arranged by size:
1399 730 611 477 190 162 116 93
74 62
BspEI T'CCGG_A
Cuts at: 3134 3134
Size: 3914
BspHI T'CATG_A
Cuts at: 3090 3090
Size: 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 472 964 1178 1480 1861 472
Size: 492 214 302 381 2525
Fragments arranged by size:
2525 492 381 302 214
BsrBI CCG'CTC
Cuts at: 50 729 2206 2260 3744 3909 50
Size: 679 1477 54 1484 165 55
Fragments arranged by size:
1484 1477 679 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1826 1826
Size: 3914
BsrFI r'CCGG_y
Cuts at: 1912 2093 2302 1912
Size: 181 209 3524
Fragments arranged by size:
3524 209 181
BssSI C'ACGA_G
Cuts at: 2185 2543 2185
Size: 358 3556
BstAPI GCAn_nnn'nTGC
Cuts at: 557 1291 557
Size: 734 3180
BstBI TT'CG_AA
Cuts at: 2275 2275
Size: 3914
BstEII G'GTnAC_C
Cuts at: 426 426
Size: 3914
BstXI CCAn_nnnn'nTGG
Cuts at: 852 852
Size: 3914
BstYI r'GATC_y
Cuts at: 1 194 692 939 1406 1429 1764 2010 3022
Size: 193 498 247 467 23 335 246 1012
Cuts at: 3022 3108 3120 3466 1
Size: 86 12 346 449
Fragments arranged by size:
1012 498 467 449 346 335 247 246
193 86 23 12
BtgI C'CryG_G
Cuts at: 399 696 857 399
Size: 297 161 3456
Fragments arranged by size:
3456 297 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1864 2045 2208 1864
Size: 181 163 3570
Fragments arranged by size:
3570 181 163
BtsI GCAGTG_nn'
Cuts at: 1157 1157
Size: 3914
DraI TTT'AAA
Cuts at: 913 913
Size: 3914
DrdI GACnn_nn'nnGTC
Cuts at: 1620 2478 1620
Size: 858 3056
EaeI y'GGCC_r
Cuts at: 260 854 1499 1673 2064 2091 3436 260
Size: 594 645 174 391 27 1345 738
Fragments arranged by size:
1345 738 645 594 391 174 27
EagI C'GGCC_G
Cuts at: 1499 1499
Size: 3914
EarI CTCTTCn'nnn_
Cuts at: 1937 2147 1937
Size: 210 3704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2122 2432 2578 3724 2122
Size: 310 146 1146 2312
Fragments arranged by size:
2312 1146 310 146
EcoICRI GAG'CTC
Cuts at: 171 926 171
Size: 755 3159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 380 694 823 1739 2171 2918 3186 183
Size: 197 314 129 916 432 747 268 911
Fragments arranged by size:
916 911 747 432 314 268 197 129
EcoNI CCTnn'n_nnAGG
Cuts at: 889 889
Size: 3914
EcoO109I rG'GnC_Cy
Cuts at: 332 1053 332
Size: 721 3193
EcoRI G'AATT_C
Cuts at: 918 918
Size: 3914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3914
FspI TGC'GCA
Cuts at: 412 835 1695 412
Size: 423 860 2631
Fragments arranged by size:
2631 860 423
HaeII r_GCGC'y
Cuts at: 666 1348 1596 2618 3597 666
Size: 682 248 1022 979 983
Fragments arranged by size:
1022 983 979 682 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1756 1786 1788 1818 1756
Size: 30 2 30 3852
Fragments arranged by size:
3852 30 30 2
HincII GTy'rAC
Cuts at: 953 953
Size: 3914
KasI G'GCGC_C
Cuts at: 1344 1592 1344
Size: 248 3666
KpnI G_GTAC'C
Cuts at: 934 934
Size: 3914
MluI A'CGCG_T
Cuts at: 716 3347 716
Size: 2631 1283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2769 2585
Size: 184 3730
MscI TGG'CCA
Cuts at: 1675 1675
Size: 3914
MslI CAynn'nnrTG
Cuts at: 418 616 1903 2030 418
Size: 198 1287 127 2302
Fragments arranged by size:
2302 1287 198 127
MspA1I CmG'CkG
Cuts at: 282 351 828 1025 1339 1699 2712 2957 282
Size: 69 477 197 314 360 1013 245 1239
Fragments arranged by size:
1239 1013 477 360 314 245 197 69
NaeI GCC'GGC
Cuts at: 2095 2095
Size: 3914
NarI GG'CG_CC
Cuts at: 1345 1593 1345
Size: 248 3666
NcoI C'CATG_G
Cuts at: 399 696 399
Size: 297 3617
NgoMIV G'CCGG_C
Cuts at: 2093 2093
Size: 3914
NspI r_CATG'y
Cuts at: 504 967 1467 2374 504
Size: 463 500 907 2044
Fragments arranged by size:
2044 907 500 463
PciI A'CATG_T
Cuts at: 2370 2370
Size: 3914
PmeI GTTT'AAAC
Cuts at: 913 913
Size: 3914
PmlI CAC'GTG
Cuts at: 149 149
Size: 3914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 3882
PspOMI G'GGCC_C
Cuts at: 331 331
Size: 3914
PstI C_TGCA'G
Cuts at: 893 961 1646 893
Size: 68 685 3161
Fragments arranged by size:
3161 685 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3914
PvuII CAG'CTG
Cuts at: 351 1339 1699 351
Size: 988 360 2566
Fragments arranged by size:
2566 988 360
RsrII CG'GwC_CG
Cuts at: 2109 2109
Size: 3914
SacI G_AGCT'C
Cuts at: 173 928 173
Size: 755 3159
SalI G'TCGA_C
Cuts at: 951 951
Size: 3914
SapI GCTCTTCn'nnn_
Cuts at: 1937 2147 1937
Size: 210 3704
SbfI CC_TGCA'GG
Cuts at: 961 961
Size: 3914
ScaI AGT'ACT
Cuts at: 3431 3431
Size: 3914
SexAI A'CCwGG_T
Cuts at: 905 905
Size: 3914
SfcI C'TryA_G
Cuts at: 33 315 865 889 957 1642 2635 2826 33
Size: 282 550 24 68 685 993 191 1121
Fragments arranged by size:
1121 993 685 550 282 191 68 24
SfoI GGC'GCC
Cuts at: 1346 1594 1346
Size: 248 3666
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
SmaI CCC'GGG
Cuts at: 330 936 330
Size: 606 3308
SmlI C'TyrA_G
Cuts at: 589 685 1069 1984 2476 2738 589
Size: 96 384 915 492 262 1765
Fragments arranged by size:
1765 915 492 384 262 96
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3914
SphI G_CATG'C
Cuts at: 504 967 1467 504
Size: 463 500 2951
Fragments arranged by size:
2951 500 463
StuI AGG'CCT
Cuts at: 484 652 484
Size: 168 3746
StyI C'CwwG_G
Cuts at: 399 432 696 1048 1402 399
Size: 33 264 352 354 2911
Fragments arranged by size:
2911 354 352 264 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1497 1630 2089 2298 1497
Size: 133 459 209 3113
Fragments arranged by size:
3113 459 209 133
TatI w'GTAC_w
Cuts at: 3429 3429
Size: 3914
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 3882
Tth111I GACn'n_nGTC
Cuts at: 1711 1711
Size: 3914
XbaI T'CTAG_A
Cuts at: 945 945
Size: 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1409 1409
Size: 3914
XmaI C'CCGG_G
Cuts at: 328 934 328
Size: 606 3308
XmnI GAAnn'nnTTC
Cuts at: 484 568 484
Size: 84 3830
ZraI GAC'GTC
Cuts at: 534 534
Size: 3914
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI