Restriction map for
pF1KB7551
(Circular) (Six-base) MAPSORT of: pF1KB7551.seq Check: 9715 from: 1 to: 3704
pF1KB7551 3704 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1529 1961 2708 2976 437
Size: 1092 432 747 268 1165
Fragments arranged by size:
1165 1092 747 432 268
AflIII A'CryG_T
Cuts at: 1056 2160 3137 1056
Size: 1104 977 1623
Fragments arranged by size:
1623 1104 977
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AlwNI CAG_nnn'CTG
Cuts at: 1009 2576 1009
Size: 1567 2137
ApaLI G'TGCA_C
Cuts at: 2474 2474
Size: 3704
ApoI r'AATT_y
Cuts at: 365 546 708 3057 3313 365
Size: 181 162 2349 256 756
Fragments arranged by size:
2349 756 256 181 162
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 724 724
Size: 3704
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 720 1134 1382 1417 720
Size: 414 248 35 3007
Fragments arranged by size:
3007 414 248 35
BanII G_rGCy'C
Cuts at: 718 1748 718
Size: 1030 2674
BbeI G_GCGC'C
Cuts at: 1138 1386 1138
Size: 248 3456
BbsI GAAGACnn'nnnn_
Cuts at: 593 593
Size: 3704
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 816 816
Size: 3704
Bme1580I G_kGCm'C
Cuts at: 1329 1422 2478 1329
Size: 93 1056 2555
Fragments arranged by size:
2555 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 3704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 601
Size: 3704
Bpu10I CC'TnA_GC
Cuts at: 527 527
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1759 2251 2549 880
Size: 879 492 298 2035
Fragments arranged by size:
2035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 406 3408 406
Size: 3002 702
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 1135 1383 2085 3581 1135
Size: 248 702 1496 1258
Fragments arranged by size:
1496 1258 702 248
BsaWI w'CCGG_w
Cuts at: 893 1414 2092 2366 2513 2924 893
Size: 521 678 274 147 411 1673
Fragments arranged by size:
1673 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1129 1887 2464 2960 1129
Size: 758 577 496 1873
Fragments arranged by size:
1873 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 1246 1292 2500 104
Size: 12 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46 12
BsiHKAI G_wGCw'C
Cuts at: 718 1496 1686 2478 718
Size: 778 190 792 1944
Fragments arranged by size:
1944 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 718 1329 1422 1496 1686 1748 2478 718
Size: 611 93 74 190 62 730 1944
Fragments arranged by size:
1944 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 218 2880 218
Size: 2662 1042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 968 1270 1651 754
Size: 214 302 381 2807
Fragments arranged by size:
2807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1996 2050 3534 3699 50
Size: 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 1616
Size: 3704
BsrFI r'CCGG_y
Cuts at: 1702 1883 2092 1702
Size: 181 209 3314
Fragments arranged by size:
3314 209 181
BssSI C'ACGA_G
Cuts at: 1975 2333 1975
Size: 358 3346
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 1081
Size: 3704
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstXI CCAn_nnnn'nTGG
Cuts at: 408 408
Size: 3704
BstYI r'GATC_y
Cuts at: 1 729 1196 1219 1554 1800 2812 2898 2910
Size: 728 467 23 335 246 1012 86 12
Cuts at: 2910 3256 1
Size: 346 449
Fragments arranged by size:
1012 728 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 185 185
Size: 3704
BtgI C'CryG_G
Cuts at: 107 107
Size: 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 947 947
Size: 3704
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 113 146 635 1289 1463 1854 1881 3226 113
Size: 33 489 654 174 391 27 1345 591
Fragments arranged by size:
1345 654 591 489 391 174 33 27
EagI C'GGCC_G
Cuts at: 113 1289 113
Size: 1176 2528
EarI CTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2222 2368 3514 1912
Size: 310 146 1146 2102
Fragments arranged by size:
2102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 601 1529 1961 2708 2976 437
Size: 164 928 432 747 268 1165
Fragments arranged by size:
1165 928 747 432 268 164
EcoO109I rG'GnC_Cy
Cuts at: 843 843
Size: 3704
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1723 1723
Size: 3704
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 1138 1386 2408 3387 1138
Size: 248 1022 979 1455
Fragments arranged by size:
1455 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1546 1576 1578 1608 1546
Size: 30 2 30 3642
Fragments arranged by size:
3642 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
HindIII A'AGCT_T
Cuts at: 449 449
Size: 3704
KasI G'GCGC_C
Cuts at: 1134 1382 1134
Size: 248 3456
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 518 2375 2559 518
Size: 1857 184 1663
Fragments arranged by size:
1857 1663 184
MscI TGG'CCA
Cuts at: 148 637 1465 148
Size: 489 828 2387
Fragments arranged by size:
2387 828 489
MslI CAynn'nnrTG
Cuts at: 1693 1820 1693
Size: 127 3577
MspA1I CmG'CkG
Cuts at: 815 1129 1489 2502 2747 815
Size: 314 360 1013 245 1772
Fragments arranged by size:
1772 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 1135 1383 1135
Size: 248 3456
NcoI C'CATG_G
Cuts at: 107 107
Size: 3704
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 381 757 1257 2164 381
Size: 376 500 907 1921
Fragments arranged by size:
1921 907 500 376
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PsiI TTA'TAA
Cuts at: 647 647
Size: 3704
PstI C_TGCA'G
Cuts at: 246 751 1436 246
Size: 505 685 2514
Fragments arranged by size:
2514 685 505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 1129 1489 1129
Size: 360 3344
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 242 747 1432 2425 2616 33
Size: 209 505 685 993 191 1121
Fragments arranged by size:
1121 993 685 505 209 191
SfoI GGC'GCC
Cuts at: 1136 1384 1136
Size: 248 3456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 859 1774 2266 2528 859
Size: 915 492 262 2035
Fragments arranged by size:
2035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
StyI C'CwwG_G
Cuts at: 107 838 1192 107
Size: 731 354 2619
Fragments arranged by size:
2619 731 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1287 1420 1879 2088 1287
Size: 133 459 209 2903
Fragments arranged by size:
2903 459 209 133
TatI w'GTAC_w
Cuts at: 3219 3219
Size: 3704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 405 423 1199 405
Size: 18 776 2910
Fragments arranged by size:
2910 776 18
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI