Restriction map for
pF1KB7554
(Circular) (Six-base) MAPSORT of: pF1KB7554.seq Check: 6150 from: 1 to: 3923
pF1KB7554 3923 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 749 1228 2795 749
Size: 479 1567 1877
Fragments arranged by size:
1877 1567 479
ApaI G_GGCC'C
Cuts at: 233 403 233
Size: 170 3753
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 571 927 3276 3532 571
Size: 356 2349 256 962
Fragments arranged by size:
2349 962 356 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 124 601 943 124
Size: 477 342 3104
Fragments arranged by size:
3104 477 342
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 139 150 939 1353 1601 1636 139
Size: 11 789 414 248 35 2426
Fragments arranged by size:
2426 789 414 248 35 11
BanII G_rGCy'C
Cuts at: 233 323 403 490 937 1967 233
Size: 90 80 87 447 1030 2189
Fragments arranged by size:
2189 1030 447 90 87 80
BbeI G_GCGC'C
Cuts at: 143 1357 1605 143
Size: 1214 248 2461
Fragments arranged by size:
2461 1214 248
BbsI GAAGACnn'nnnn_
Cuts at: 845 845
Size: 3923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3889
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 753 870 1443 753
Size: 117 573 3233
Fragments arranged by size:
3233 573 117
BglI GCCn_nnn'nGGC
Cuts at: 367 367
Size: 3923
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 634 1035 634
Size: 401 3522
Bme1580I G_kGCm'C
Cuts at: 233 403 1548 1641 2697 233
Size: 170 1145 93 1056 1459
Fragments arranged by size:
1459 1145 1056 170 93
BmrI ACTGGGnnnn_n'
Cuts at: 767 1135 1216 1551 3695 767
Size: 368 81 335 2144 995
Fragments arranged by size:
2144 995 368 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 583 935 504
Size: 79 352 3492
Fragments arranged by size:
3492 352 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2470 2768 1099
Size: 879 492 298 2254
Fragments arranged by size:
2254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 869 1437 3474 6
Size: 863 568 2037 455
Fragments arranged by size:
2037 863 568 455
BsaHI Gr'CG_yC
Cuts at: 140 196 1354 1602 2304 3800 140
Size: 56 1158 248 702 1496 263
Fragments arranged by size:
1496 1158 702 263 248 56
BsaWI w'CCGG_w
Cuts at: 302 1112 1633 2311 2585 2732 3143 302
Size: 810 521 678 274 147 411 1082
Fragments arranged by size:
1082 810 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 699 264
Size: 435 3488
BseYI C'CCAG_C
Cuts at: 1348 2106 2683 3179 1348
Size: 758 577 496 2092
Fragments arranged by size:
2092 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 937 1715 1905 2697 937
Size: 778 190 792 2163
Fragments arranged by size:
2163 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 571 571
Size: 3923
Bsp1286I G_dGCh'C
Cuts at: 233 323 403 490 937 1548 1641 1715 1905
Size: 90 80 87 447 611 93 74 190
Cuts at: 1905 1967 2697 233
Size: 62 730 1459
Fragments arranged by size:
1459 730 611 447 190 93 90 87
80 74 62
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1187 1489 1870 973
Size: 214 302 381 3026
Fragments arranged by size:
3026 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 129 1921 2102 2311 129
Size: 1792 181 209 1741
Fragments arranged by size:
1792 1741 209 181
BssSI C'ACGA_G
Cuts at: 289 2194 2552 289
Size: 1905 358 1660
Fragments arranged by size:
1905 1660 358
BstAPI GCAn_nnn'nTGC
Cuts at: 447 768 1300 447
Size: 321 532 3070
Fragments arranged by size:
3070 532 321
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstXI CCAn_nnnn'nTGG
Cuts at: 561 561
Size: 3923
BstYI r'GATC_y
Cuts at: 1 948 1415 1438 1773 2019 3031 3117 3129
Size: 947 467 23 335 246 1012 86 12
Cuts at: 3129 3475 1
Size: 346 449
Fragments arranged by size:
1012 947 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 143 225 418 143
Size: 82 193 3648
Fragments arranged by size:
3648 193 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 368 525 617 1508 1682 2073 2100 3445 368
Size: 157 92 891 174 391 27 1345 846
Fragments arranged by size:
1345 891 846 391 174 157 92 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 345 442 587 2131 2441 2587 3733 345
Size: 97 145 1544 310 146 1146 535
Fragments arranged by size:
1544 1146 535 310 146 145 97
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 583 935 1748 2180 2927 3195 504
Size: 79 352 813 432 747 268 1232
Fragments arranged by size:
1232 813 747 432 352 268 79
EcoO109I rG'GnC_Cy
Cuts at: 399 400 1062 399
Size: 1 662 3260
Fragments arranged by size:
3260 662 1
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 143 1357 1605 2627 3606 143
Size: 1214 248 1022 979 460
Fragments arranged by size:
1214 1022 979 460 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 597 629 832 864 1765 1795 1797 1827 597
Size: 32 203 32 901 30 2 30 2693
Fragments arranged by size:
2693 901 203 32 32 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 139 1353 1601 139
Size: 1214 248 2461
Fragments arranged by size:
2461 1214 248
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 2594 2778 566
Size: 2028 184 1711
Fragments arranged by size:
2028 1711 184
MscI TGG'CCA
Cuts at: 619 1684 619
Size: 1065 2858
MslI CAynn'nnrTG
Cuts at: 1912 2039 1912
Size: 127 3796
MspA1I CmG'CkG
Cuts at: 156 174 227 373 420 1034 1348 1708 2721
Size: 18 53 146 47 614 314 360 1013
Cuts at: 2721 2966 156
Size: 245 1113
Fragments arranged by size:
1113 1013 614 360 314 245 146 53
47 18
NaeI GCC'GGC
Cuts at: 131 2104 131
Size: 1973 1950
NarI GG'CG_CC
Cuts at: 140 1354 1602 140
Size: 1214 248 2461
Fragments arranged by size:
2461 1214 248
NgoMIV G'CCGG_C
Cuts at: 129 2102 129
Size: 1973 1950
NspI r_CATG'y
Cuts at: 976 1476 2383 976
Size: 500 907 2516
Fragments arranged by size:
2516 907 500
PasI CC'CwG_GG
Cuts at: 465 465
Size: 3923
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PspOMI G'GGCC_C
Cuts at: 229 399 229
Size: 170 3753
PstI C_TGCA'G
Cuts at: 632 970 1655 632
Size: 338 685 2900
Fragments arranged by size:
2900 685 338
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 1348 1708 1348
Size: 360 3563
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SacII CC_GC'GG
Cuts at: 228 421 228
Size: 193 3730
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SexAI A'CCwGG_T
Cuts at: 655 655
Size: 3923
SfcI C'TryA_G
Cuts at: 33 628 966 1651 2644 2835 33
Size: 595 338 685 993 191 1121
Fragments arranged by size:
1121 993 685 595 338 191
SfoI GGC'GCC
Cuts at: 141 1355 1603 141
Size: 1214 248 2461
Fragments arranged by size:
2461 1214 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 126 945 126
Size: 819 3104
SmlI C'TyrA_G
Cuts at: 1078 1993 2485 2747 1078
Size: 915 492 262 2254
Fragments arranged by size:
2254 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StuI AGG'CCT
Cuts at: 504 504
Size: 3923
StyI C'CwwG_G
Cuts at: 1057 1411 1057
Size: 354 3569
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1506 1639 2098 2307 1506
Size: 133 459 209 3122
Fragments arranged by size:
3122 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 3891
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 124 943 124
Size: 819 3104
XmnI GAAnn'nnTTC
Cuts at: 571 571
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI