Restriction map for
pF1KB7556
(Circular) (Six-base) MAPSORT of: pF1KB7556.seq Check: 2819 from: 1 to: 3926
pF1KB7556 3926 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 964 964
Size: 3926
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1751 2183 2930 3198 1751
Size: 432 747 268 2479
Fragments arranged by size:
2479 747 432 268
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 255 730 1231 2798 255
Size: 475 501 1567 1383
Fragments arranged by size:
1567 1383 501 475
ApaI G_GGCC'C
Cuts at: 290 363 290
Size: 73 3853
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 930 3279 3535 930
Size: 2349 256 1321
Fragments arranged by size:
2349 1321 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 135 946 135
Size: 811 3115
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 219 670 942 1356 1604 1639 219
Size: 451 272 414 248 35 2506
Fragments arranged by size:
2506 451 414 272 248 35
BanII G_rGCy'C
Cuts at: 142 290 363 940 1970 142
Size: 148 73 577 1030 2098
Fragments arranged by size:
2098 1030 577 148 73
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbsI GAAGACnn'nnnn_
Cuts at: 801 801
Size: 3926
BbvCI CC'TCA_GC
Cuts at: 843 843
Size: 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 602 636 602
Size: 34 3892
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 1038 1038
Size: 3926
Bme1580I G_kGCm'C
Cuts at: 290 363 1551 1644 2700 290
Size: 73 1188 93 1056 1516
Fragments arranged by size:
1516 1188 1056 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 359
Size: 3926
Bpu10I CC'TnA_GC
Cuts at: 830 843 830
Size: 13 3913
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 869 3630 869
Size: 2761 1165
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 1357 1605 2307 3803 1357
Size: 248 702 1496 1480
Fragments arranged by size:
1496 1480 702 248
BsaWI w'CCGG_w
Cuts at: 479 1115 1636 2314 2588 2735 3146 479
Size: 636 521 678 274 147 411 1259
Fragments arranged by size:
1259 678 636 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 244 274 244
Size: 30 3896
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 577 901 436
Size: 141 324 3461
Fragments arranged by size:
3461 324 141
BseYI C'CCAG_C
Cuts at: 200 289 799 820 1351 2109 2686 3182 200
Size: 89 510 21 531 758 577 496 944
Fragments arranged by size:
944 758 577 531 510 496 89 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 736 492
Size: 244 3682
BsiEI CG_ry'CG
Cuts at: 104 185 1468 1514 2722 104
Size: 81 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 81 46
BsiHKAI G_wGCw'C
Cuts at: 142 940 1718 1908 2700 142
Size: 798 778 190 792 1368
Fragments arranged by size:
1368 798 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 142 290 363 940 1551 1644 1718 1908 1970
Size: 148 73 577 611 93 74 190 62
Cuts at: 1970 2700 142
Size: 730 1368
Fragments arranged by size:
1368 730 611 577 190 148 93 74
73 62
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 767 3102 767
Size: 2335 1591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 976 1190 1492 1873 976
Size: 214 302 381 3029
Fragments arranged by size:
3029 381 302 214
BsrBI CCG'CTC
Cuts at: 50 447 2218 2272 3756 3921 50
Size: 397 1771 54 1484 165 55
Fragments arranged by size:
1771 1484 397 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1838
Size: 3926
BsrFI r'CCGG_y
Cuts at: 1924 2105 2314 1924
Size: 181 209 3536
Fragments arranged by size:
3536 209 181
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 1303 1303
Size: 3926
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstYI r'GATC_y
Cuts at: 1 951 1418 1441 1776 2022 3034 3120 3132
Size: 950 467 23 335 246 1012 86 12
Cuts at: 3132 3478 1
Size: 346 449
Fragments arranged by size:
1012 950 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 743 743
Size: 3926
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 835 1876 2057 2220 835
Size: 1041 181 163 2541
Fragments arranged by size:
2541 1041 181 163
BtsI GCAGTG_nn'
Cuts at: 265 748 1169 265
Size: 483 421 3022
Fragments arranged by size:
3022 483 421
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DraIII CAC_nnn'GTG
Cuts at: 219 219
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 182 343 464 1511 1685 2076 2103 3448 182
Size: 161 121 1047 174 391 27 1345 660
Fragments arranged by size:
1345 1047 660 391 174 161 121 27
EagI C'GGCC_G
Cuts at: 182 1511 182
Size: 1329 2597
EarI CTCTTCn'nnn_
Cuts at: 413 1949 2159 413
Size: 1536 210 2180
Fragments arranged by size:
2180 1536 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 526 2134 2444 2590 3736 526
Size: 1608 310 146 1146 716
Fragments arranged by size:
1608 1146 716 310 146
EcoICRI GAG'CTC
Cuts at: 140 938 140
Size: 798 3128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 1751 2183 2930 3198 359
Size: 1392 432 747 268 1087
Fragments arranged by size:
1392 1087 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 286 360 727 1065 286
Size: 74 367 338 3147
Fragments arranged by size:
3147 367 338 74
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 429 588 1360 1608 2630 3609 429
Size: 159 772 248 1022 979 746
Fragments arranged by size:
1022 979 772 746 248 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 303 335 523 555 868 900 1768
Size: 32 40 32 188 32 313 32 868
Cuts at: 1768 1798 1800 1830 231
Size: 30 2 30 2327
Fragments arranged by size:
2327 868 313 188 40 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 928 2597 2781 928
Size: 1669 184 2073
Fragments arranged by size:
2073 1669 184
MscI TGG'CCA
Cuts at: 1687 1687
Size: 3926
MslI CAynn'nnrTG
Cuts at: 766 1915 2042 766
Size: 1149 127 2650
Fragments arranged by size:
2650 1149 127
MspA1I CmG'CkG
Cuts at: 872 1037 1351 1711 2724 2969 872
Size: 165 314 360 1013 245 1829
Fragments arranged by size:
1829 1013 360 314 245 165
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 979 1479 2386 979
Size: 500 907 2519
Fragments arranged by size:
2519 907 500
PasI CC'CwG_GG
Cuts at: 363 724 363
Size: 361 3565
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PfoI T'CCnGG_A
Cuts at: 142 629 142
Size: 487 3439
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PspOMI G'GGCC_C
Cuts at: 286 359 286
Size: 73 3853
PstI C_TGCA'G
Cuts at: 260 509 551 572 973 1658 260
Size: 249 42 21 401 685 2528
Fragments arranged by size:
2528 685 401 249 42 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 1351 1711 1351
Size: 360 3566
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 142 940 142
Size: 798 3128
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 509 973 509
Size: 464 3462
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SexAI A'CCwGG_T
Cuts at: 215 215
Size: 3926
SfcI C'TryA_G
Cuts at: 33 256 505 547 568 969 1654 2647 2838
Size: 223 249 42 21 401 685 993 191
Cuts at: 2838 33
Size: 1121
Fragments arranged by size:
1121 993 685 401 249 223 191 42
21
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 948 948
Size: 3926
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StuI AGG'CCT
Cuts at: 306 393 306
Size: 87 3839
StyI C'CwwG_G
Cuts at: 641 894 1060 1414 641
Size: 253 166 354 3153
Fragments arranged by size:
3153 354 253 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 511 1509 1642 2101 2310 511
Size: 998 133 459 209 2127
Fragments arranged by size:
2127 998 459 209 133
TatI w'GTAC_w
Cuts at: 323 3441 323
Size: 3118 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 3443 3475 244
Size: 32 3167 32 695
Fragments arranged by size:
3167 695 32 32
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1421 1421
Size: 3926
XmaI C'CCGG_G
Cuts at: 946 946
Size: 3926
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI