Restriction map for
pF1KB7559
(Circular) (Six-base) MAPSORT of: pF1KB7559.seq Check: 33 from: 1 to: 3731
pF1KB7559 3731 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 346 590 346
Size: 244 3487
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 747 747
Size: 3731
AclI AA'CG_TT
Cuts at: 3571 3571
Size: 3731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1556 1988 2735 3003 1556
Size: 432 747 268 2284
Fragments arranged by size:
2284 747 432 268
AfeI AGC'GCT
Cuts at: 280 280
Size: 3731
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AhdI GACnn_n'nnGTC
Cuts at: 240 240
Size: 3731
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 625 2501 625
Size: 1876 1855
ApoI r'AATT_y
Cuts at: 735 3084 3340 735
Size: 2349 256 1126
Fragments arranged by size:
2349 1126 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 660 751 660
Size: 91 3640
BamHI G'GATC_C
Cuts at: 756 756
Size: 3731
BanI G'GyrC_C
Cuts at: 516 747 1161 1409 1444 516
Size: 231 414 248 35 2803
Fragments arranged by size:
2803 414 248 231 35
BanII G_rGCy'C
Cuts at: 121 406 745 1775 121
Size: 285 339 1030 2077
Fragments arranged by size:
2077 1030 339 285
BbeI G_GCGC'C
Cuts at: 1165 1413 1165
Size: 248 3483
BbsI GAAGACnn'nnnn_
Cuts at: 672 672
Size: 3731
BciVI GTATCCnnnnn_n'
Cuts at: 1629 2390 3731 1629
Size: 761 1341 1629
Fragments arranged by size:
1629 1341 761
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglII A'GATC_T
Cuts at: 1246 3283 1246
Size: 2037 1694
BlpI GC'TnA_GC
Cuts at: 405 475 843 405
Size: 70 368 3293
Fragments arranged by size:
3293 368 70
Bme1580I G_kGCm'C
Cuts at: 295 462 521 629 1356 1449 2505 295
Size: 167 59 108 727 93 1056 1521
Fragments arranged by size:
1521 1056 727 167 108 93 59
BmrI ACTGGGnnnn_n'
Cuts at: 224 625 943 1024 1359 3503 224
Size: 401 318 81 335 2144 452
Fragments arranged by size:
2144 452 401 335 318 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 3731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 474
Size: 3731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 907 1786 2278 2576 481
Size: 426 879 492 298 1636
Fragments arranged by size:
1636 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 663 3435 663
Size: 2772 959
BsaAI yAC'GTr
Cuts at: 624 1084 624
Size: 460 3271
BsaBI GATnn'nnATC
Cuts at: 6 1245 3282 6
Size: 1239 2037 455
Fragments arranged by size:
2037 1239 455
BsaHI Gr'CG_yC
Cuts at: 1162 1410 2112 3608 1162
Size: 248 702 1496 1285
Fragments arranged by size:
1496 1285 702 248
BsaWI w'CCGG_w
Cuts at: 132 669 920 1441 2119 2393 2540 2951 132
Size: 537 251 521 678 274 147 411 912
Fragments arranged by size:
912 678 537 521 411 274 251 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 265 268 286 289 151
Size: 114 3 18 3 3593
Fragments arranged by size:
3593 114 18 3 3
BseYI C'CCAG_C
Cuts at: 340 1156 1914 2491 2987 340
Size: 816 758 577 496 1084
Fragments arranged by size:
1084 816 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 629 745 1523 1713 2505 629
Size: 116 778 190 792 1855
Fragments arranged by size:
1855 792 778 190 116
Bsp1286I G_dGCh'C
Cuts at: 121 295 406 462 521 629 745 1356 1449
Size: 174 111 56 59 108 116 611 93
Cuts at: 1449 1523 1713 1775 2505 121
Size: 74 190 62 730 1347
Fragments arranged by size:
1347 730 611 190 174 116 111 108
93 74 62 59 56
BspEI T'CCGG_A
Cuts at: 132 2951 132
Size: 2819 912
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 346 590 781 995 1297 1678 346
Size: 244 191 214 302 381 2399
Fragments arranged by size:
2399 381 302 244 214 191
BsrBI CCG'CTC
Cuts at: 50 2023 2077 3561 3726 50
Size: 1973 54 1484 165 55
Fragments arranged by size:
1973 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1643 1643
Size: 3731
BsrFI r'CCGG_y
Cuts at: 298 1729 1910 2119 298
Size: 1431 181 209 1910
Fragments arranged by size:
1910 1431 209 181
BssSI C'ACGA_G
Cuts at: 2002 2360 2002
Size: 358 3373
BstAPI GCAn_nnn'nTGC
Cuts at: 594 1108 594
Size: 514 3217
BstBI TT'CG_AA
Cuts at: 2092 2092
Size: 3731
BstYI r'GATC_y
Cuts at: 1 756 1223 1246 1581 1827 2839 2925 2937
Size: 755 467 23 335 246 1012 86 12
Cuts at: 2937 3283 1
Size: 346 449
Fragments arranged by size:
1012 755 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 113 201 113
Size: 88 3643
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 558 1681 1862 2025 558
Size: 1123 181 163 2264
Fragments arranged by size:
2264 1123 181 163
BtsI GCAGTG_nn'
Cuts at: 974 974
Size: 3731
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DraIII CAC_nnn'GTG
Cuts at: 633 633
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 395 440 646 1316 1490 1881 1908 3253 395
Size: 45 206 670 174 391 27 1345 873
Fragments arranged by size:
1345 873 670 391 206 174 45 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1939 2249 2395 3541 1939
Size: 310 146 1146 2129
Fragments arranged by size:
2129 1146 310 146
EcoICRI GAG'CTC
Cuts at: 743 743
Size: 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 1556 1988 2735 3003 474
Size: 1082 432 747 268 1202
Fragments arranged by size:
1202 1082 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 186 489 186
Size: 303 3428
EcoO109I rG'GnC_Cy
Cuts at: 258 870 258
Size: 612 3119
EcoRI G'AATT_C
Cuts at: 735 735
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1750 1750
Size: 3731
FspI TGC'GCA
Cuts at: 1512 1512
Size: 3731
HaeII r_GCGC'y
Cuts at: 282 1165 1413 2435 3414 282
Size: 883 248 1022 979 599
Fragments arranged by size:
1022 979 883 599 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 226 258 1573 1603 1605 1635 226
Size: 32 1315 30 2 30 2322
Fragments arranged by size:
2322 1315 32 30 30 2
HincII GTy'rAC
Cuts at: 770 770
Size: 3731
KasI G'GCGC_C
Cuts at: 1161 1409 1161
Size: 248 3483
KpnI G_GTAC'C
Cuts at: 751 751
Size: 3731
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2586 2402
Size: 184 3547
MscI TGG'CCA
Cuts at: 648 1492 648
Size: 844 2887
MslI CAynn'nnrTG
Cuts at: 1720 1847 1720
Size: 127 3604
MspA1I CmG'CkG
Cuts at: 162 203 432 591 724 842 1156 1516 2529
Size: 41 229 159 133 118 314 360 1013
Cuts at: 2529 2774 162
Size: 245 1119
Fragments arranged by size:
1119 1013 360 314 245 229 159 133
118 41
NaeI GCC'GGC
Cuts at: 300 1912 300
Size: 1612 2119
NarI GG'CG_CC
Cuts at: 1162 1410 1162
Size: 248 3483
NgoMIV G'CCGG_C
Cuts at: 298 1910 298
Size: 1612 2119
NspI r_CATG'y
Cuts at: 784 1284 2191 784
Size: 500 907 2324
Fragments arranged by size:
2324 907 500
PasI CC'CwG_GG
Cuts at: 512 512
Size: 3731
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 3699
PpuMI rG'GwC_Cy
Cuts at: 258 258
Size: 3731
PstI C_TGCA'G
Cuts at: 161 778 1463 161
Size: 617 685 2429
Fragments arranged by size:
2429 685 617
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 591 1156 1516 591
Size: 565 360 2806
Fragments arranged by size:
2806 565 360
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 745 745
Size: 3731
SacII CC_GC'GG
Cuts at: 204 204
Size: 3731
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 778 778
Size: 3731
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SfcI C'TryA_G
Cuts at: 33 157 774 1459 2452 2643 33
Size: 124 617 685 993 191 1121
Fragments arranged by size:
1121 993 685 617 191 124
SfoI GGC'GCC
Cuts at: 1163 1411 1163
Size: 248 3483
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 496 886 1801 2293 2555 496
Size: 390 915 492 262 1672
Fragments arranged by size:
1672 915 492 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3731
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StyI C'CwwG_G
Cuts at: 398 865 1219 398
Size: 467 354 2910
Fragments arranged by size:
2910 467 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 504 644 1314 1447 1906 2115 504
Size: 140 670 133 459 209 2120
Fragments arranged by size:
2120 670 459 209 140 133
TatI w'GTAC_w
Cuts at: 3246 3246
Size: 3731
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 3699
Tth111I GACn'n_nGTC
Cuts at: 1528 1528
Size: 3731
XbaI T'CTAG_A
Cuts at: 762 762
Size: 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1226 1226
Size: 3731
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI