Restriction map for
pF1KB7561
(Circular) (Six-base) MAPSORT of: pF1KB7561.seq Check: 2431 from: 1 to: 3734
pF1KB7561 3734 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 475 772 475
Size: 297 3437
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 505 1559 1991 2738 3006 425
Size: 80 1054 432 747 268 1153
Fragments arranged by size:
1153 1054 747 432 268 80
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AlwNI CAG_nnn'CTG
Cuts at: 1039 2606 1039
Size: 1567 2167
ApaLI G'TGCA_C
Cuts at: 2504 2504
Size: 3734
ApoI r'AATT_y
Cuts at: 526 716 738 3087 3343 526
Size: 190 22 2349 256 917
Fragments arranged by size:
2349 917 256 190 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 754 754
Size: 3734
BamHI G'GATC_C
Cuts at: 759 759
Size: 3734
BanI G'GyrC_C
Cuts at: 419 750 1164 1412 1447 419
Size: 331 414 248 35 2706
Fragments arranged by size:
2706 414 331 248 35
BanII G_rGCy'C
Cuts at: 748 1778 748
Size: 1030 2704
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 3734
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 228 1254 228
Size: 1026 2708
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 1359 1452 2508 1359
Size: 93 1056 2585
Fragments arranged by size:
2585 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 278 1248 3285 6
Size: 272 970 2037 455
Fragments arranged by size:
2037 970 455 272
BsaHI Gr'CG_yC
Cuts at: 1165 1413 2115 3611 1165
Size: 248 702 1496 1288
Fragments arranged by size:
1496 1288 702 248
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2396 2543 2954 923
Size: 521 678 274 147 411 1703
Fragments arranged by size:
1703 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 622
Size: 3734
BseYI C'CCAG_C
Cuts at: 1159 1917 2494 2990 1159
Size: 758 577 496 1903
Fragments arranged by size:
1903 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2530 104
Size: 1172 46 1208 1308
Fragments arranged by size:
1308 1208 1172 46
BsiHKAI G_wGCw'C
Cuts at: 748 1526 1716 2508 748
Size: 778 190 792 1974
Fragments arranged by size:
1974 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 748 1359 1452 1526 1716 1778 2508 748
Size: 611 93 74 190 62 730 1974
Fragments arranged by size:
1974 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 201 231 2910 201
Size: 30 2679 1025
Fragments arranged by size:
2679 1025 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 1732 1913 2122 1732
Size: 181 209 3344
Fragments arranged by size:
3344 209 181
BssSI C'ACGA_G
Cuts at: 2005 2363 2005
Size: 358 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 349 1111 349
Size: 762 2972
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3734
BstYI r'GATC_y
Cuts at: 1 759 1226 1249 1584 1830 2842 2928 2940
Size: 758 467 23 335 246 1012 86 12
Cuts at: 2940 3286 1
Size: 346 449
Fragments arranged by size:
1012 758 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 1319 1493 1884 1911 3256 1319
Size: 174 391 27 1345 1797
Fragments arranged by size:
1797 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 590 1757 1967 590
Size: 1167 210 2357
Fragments arranged by size:
2357 1167 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 505 1559 1991 2738 3006 425
Size: 80 1054 432 747 268 1153
Fragments arranged by size:
1153 1054 747 432 268 80
EcoO109I rG'GnC_Cy
Cuts at: 873 873
Size: 3734
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 1168 1416 2438 3417 1168
Size: 248 1022 979 1485
Fragments arranged by size:
1485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3672
Fragments arranged by size:
3672 30 30 2
HincII GTy'rAC
Cuts at: 658 773 658
Size: 115 3619
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 112 1723 1850 112
Size: 1611 127 1996
Fragments arranged by size:
1996 1611 127
MspA1I CmG'CkG
Cuts at: 634 845 1159 1519 2532 2777 634
Size: 211 314 360 1013 245 1591
Fragments arranged by size:
1591 1013 360 314 245 211
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NspI r_CATG'y
Cuts at: 787 1287 2194 787
Size: 500 907 2327
Fragments arranged by size:
2327 907 500
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PsiI TTA'TAA
Cuts at: 397 397
Size: 3734
PstI C_TGCA'G
Cuts at: 165 781 1466 165
Size: 616 685 2433
Fragments arranged by size:
2433 685 616
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 634 1159 1519 634
Size: 525 360 2849
Fragments arranged by size:
2849 525 360
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 590 1757 1967 590
Size: 1167 210 2357
Fragments arranged by size:
2357 1167 210
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 161 578 777 1462 2455 2646 33
Size: 128 417 199 685 993 191 1121
Fragments arranged by size:
1121 993 685 417 199 191 128
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StyI C'CwwG_G
Cuts at: 868 1222 868
Size: 354 3380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1317 1450 1909 2118 1317
Size: 133 459 209 2933
Fragments arranged by size:
2933 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 631 1229 631
Size: 598 3136
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
XmnI GAAnn'nnTTC
Cuts at: 362 458 362
Size: 96 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI