Restriction map for
pF1KB7563
(Circular) (Six-base) MAPSORT of: pF1KB7563.seq Check: 1681 from: 1 to: 3734
pF1KB7563 3734 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 652 652
Size: 3734
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1559 1991 2738 3006 268
Size: 1291 432 747 268 996
Fragments arranged by size:
1291 996 747 432 268
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AlwNI CAG_nnn'CTG
Cuts at: 1039 2606 1039
Size: 1567 2167
ApaLI G'TGCA_C
Cuts at: 2504 2504
Size: 3734
ApoI r'AATT_y
Cuts at: 544 738 3087 3343 544
Size: 194 2349 256 935
Fragments arranged by size:
2349 935 256 194
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 156 754 156
Size: 598 3136
BamHI G'GATC_C
Cuts at: 759 759
Size: 3734
BanI G'GyrC_C
Cuts at: 750 1164 1412 1447 750
Size: 414 248 35 3037
Fragments arranged by size:
3037 414 248 35
BanII G_rGCy'C
Cuts at: 748 1778 748
Size: 1030 2704
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BbsI GAAGACnn'nnnn_
Cuts at: 121 706 121
Size: 585 3149
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 1359 1452 2508 1359
Size: 93 1056 2585
Fragments arranged by size:
2585 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BmtI G_CTAG'C
Cuts at: 526 526
Size: 3734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 453 2101 453
Size: 1648 2086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 200 1165 1413 2115 3611 200
Size: 965 248 702 1496 323
Fragments arranged by size:
1496 965 702 323 248
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2396 2543 2954 923
Size: 521 678 274 147 411 1703
Fragments arranged by size:
1703 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 359 359
Size: 3734
BseYI C'CCAG_C
Cuts at: 1159 1917 2494 2990 1159
Size: 758 577 496 1903
Fragments arranged by size:
1903 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2530 104
Size: 1172 46 1208 1308
Fragments arranged by size:
1308 1208 1172 46
BsiHKAI G_wGCw'C
Cuts at: 748 1526 1716 2508 748
Size: 778 190 792 1974
Fragments arranged by size:
1974 792 778 190
BsiWI C'GTAC_G
Cuts at: 119 119
Size: 3734
Bsp1286I G_dGCh'C
Cuts at: 748 1359 1452 1526 1716 1778 2508 748
Size: 611 93 74 190 62 730 1974
Fragments arranged by size:
1974 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 181 652 784 998 1300 1681 181
Size: 471 132 214 302 381 2234
Fragments arranged by size:
2234 471 381 302 214 132
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 1732 1913 2122 1732
Size: 181 209 3344
Fragments arranged by size:
3344 209 181
BssSI C'ACGA_G
Cuts at: 605 2005 2363 605
Size: 1400 358 1976
Fragments arranged by size:
1976 1400 358
BstAPI GCAn_nnn'nTGC
Cuts at: 173 1111 173
Size: 938 2796
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstYI r'GATC_y
Cuts at: 1 759 1226 1249 1584 1830 2842 2928 2940
Size: 758 467 23 335 246 1012 86 12
Cuts at: 2940 3286 1
Size: 346 449
Fragments arranged by size:
1012 758 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 350 107
Size: 243 3491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 243 733 243
Size: 490 3244
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 1319 1493 1884 1911 3256 1319
Size: 174 391 27 1345 1797
Fragments arranged by size:
1797 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 136 422 444 578 1757 1967 136
Size: 286 22 134 1179 210 1903
Fragments arranged by size:
1903 1179 286 210 134 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1559 1991 2738 3006 268
Size: 1291 432 747 268 996
Fragments arranged by size:
1291 996 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 873 873
Size: 3734
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 1168 1416 2438 3417 1168
Size: 248 1022 979 1485
Fragments arranged by size:
1485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3672
Fragments arranged by size:
3672 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
HindIII A'AGCT_T
Cuts at: 280 280
Size: 3734
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MfeI C'AATT_G
Cuts at: 527 527
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 1723 1850 1723
Size: 127 3607
MspA1I CmG'CkG
Cuts at: 301 845 1159 1519 2532 2777 301
Size: 544 314 360 1013 245 1258
Fragments arranged by size:
1258 1013 544 360 314 245
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NcoI C'CATG_G
Cuts at: 107 350 107
Size: 243 3491
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NheI G'CTAG_C
Cuts at: 522 522
Size: 3734
NspI r_CATG'y
Cuts at: 436 787 1287 2194 436
Size: 351 500 907 1976
Fragments arranged by size:
1976 907 500 351
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PshAI GACnn'nnGTC
Cuts at: 498 498
Size: 3734
PstI C_TGCA'G
Cuts at: 781 1466 781
Size: 685 3049
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 422 1757 1967 422
Size: 1335 210 2189
Fragments arranged by size:
2189 1335 210
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 339 777 1462 2455 2646 33
Size: 306 438 685 993 191 1121
Fragments arranged by size:
1121 993 685 438 306 191
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 436 787 1287 436
Size: 351 500 2883
Fragments arranged by size:
2883 500 351
SspI AAT'ATT
Cuts at: 402 402
Size: 3734
StyI C'CwwG_G
Cuts at: 107 350 868 1222 107
Size: 243 518 354 2619
Fragments arranged by size:
2619 518 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1317 1450 1909 2118 1317
Size: 133 459 209 2933
Fragments arranged by size:
2933 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3734
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BpmI Bpu10I BsaXI BsgI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI