Restriction map for
pF1KB7564
(Circular) (Six-base) MAPSORT of: pF1KB7564.seq Check: 2780 from: 1 to: 3938
pF1KB7564 3938 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 976 976
Size: 3938
Acc65I G'GTAC_C
Cuts at: 954 954
Size: 3938
AclI AA'CG_TT
Cuts at: 3778 3778
Size: 3938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 709 1763 2195 2942 3210 613
Size: 96 1054 432 747 268 1341
Fragments arranged by size:
1341 1054 747 432 268 96
AfeI AGC'GCT
Cuts at: 667 667
Size: 3938
AflIII A'CryG_T
Cuts at: 1290 2394 3371 1290
Size: 1104 977 1857
Fragments arranged by size:
1857 1104 977
AgeI A'CCGG_T
Cuts at: 2326 2326
Size: 3938
AleI CACnn'nnGTG
Cuts at: 467 467
Size: 3938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 734 734
Size: 3938
AlwNI CAG_nnn'CTG
Cuts at: 1243 2810 1243
Size: 1567 2371
ApaI G_GGCC'C
Cuts at: 376 778 376
Size: 402 3536
ApaLI G'TGCA_C
Cuts at: 552 2708 552
Size: 2156 1782
ApoI r'AATT_y
Cuts at: 942 3291 3547 942
Size: 2349 256 1333
Fragments arranged by size:
2349 1333 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3938
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
AvaI C'yCGr_G
Cuts at: 238 337 352 958 238
Size: 99 15 606 3218
Fragments arranged by size:
3218 606 99 15
AvrII C'CTAG_G
Cuts at: 213 213
Size: 3938
BamHI G'GATC_C
Cuts at: 963 963
Size: 3938
BanI G'GyrC_C
Cuts at: 954 1368 1616 1651 954
Size: 414 248 35 3241
Fragments arranged by size:
3241 414 248 35
BanII G_rGCy'C
Cuts at: 173 359 376 778 952 1982 173
Size: 186 17 402 174 1030 2129
Fragments arranged by size:
2129 1030 402 186 174 17
BbeI G_GCGC'C
Cuts at: 1372 1620 1372
Size: 248 3690
BbsI GAAGACnn'nnnn_
Cuts at: 111 111
Size: 3938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3904
BciVI GTATCCnnnnn_n'
Cuts at: 1836 2597 3938 1836
Size: 761 1341 1836
Fragments arranged by size:
1836 1341 761
BclI T'GATC_A
Cuts at: 1458 1458
Size: 3938
BglII A'GATC_T
Cuts at: 1453 3490 1453
Size: 2037 1901
BlpI GC'TnA_GC
Cuts at: 1050 1050
Size: 3938
Bme1580I G_kGCm'C
Cuts at: 376 556 778 816 1563 1656 2712 376
Size: 180 222 38 747 93 1056 1602
Fragments arranged by size:
1602 1056 747 222 180 93 38
BmgBI CAC'GTC
Cuts at: 603 603
Size: 3938
BmrI ACTGGGnnnn_n'
Cuts at: 199 1150 1231 1566 3710 199
Size: 951 81 335 2144 427
Fragments arranged by size:
2144 951 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 336 2305 336
Size: 1969 1969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 697 323
Size: 374 3564
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1114 1993 2485 2783 505
Size: 609 879 492 298 1660
Fragments arranged by size:
1660 879 609 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 153 3642 153
Size: 3489 449
BsaAI yAC'GTr
Cuts at: 443 804 1291 443
Size: 361 487 3090
Fragments arranged by size:
3090 487 361
BsaBI GATnn'nnATC
Cuts at: 6 1452 3489 6
Size: 1446 2037 455
Fragments arranged by size:
2037 1446 455
BsaHI Gr'CG_yC
Cuts at: 365 1369 1617 2319 3815 365
Size: 1004 248 702 1496 488
Fragments arranged by size:
1496 1004 702 488 248
BsaWI w'CCGG_w
Cuts at: 1127 1648 2326 2600 2747 3158 1127
Size: 521 678 274 147 411 1907
Fragments arranged by size:
1907 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 778 808 778
Size: 30 3908
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 150 320 931 934 937 147
Size: 3 170 611 3 3 3148
Fragments arranged by size:
3148 611 170 3 3 3
BseYI C'CCAG_C
Cuts at: 161 662 1363 2121 2698 3194 161
Size: 501 701 758 577 496 905
Fragments arranged by size:
905 758 701 577 501 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 727 784 465
Size: 262 57 3619
Fragments arranged by size:
3619 262 57
BsiEI CG_ry'CG
Cuts at: 104 410 1480 1526 2734 104
Size: 306 1070 46 1208 1308
Fragments arranged by size:
1308 1208 1070 306 46
BsiHKAI G_wGCw'C
Cuts at: 173 556 952 1730 1920 2712 173
Size: 383 396 778 190 792 1399
Fragments arranged by size:
1399 792 778 396 383 190
BsmI GAATG_Cn'
Cuts at: 620 620
Size: 3938
Bsp1286I G_dGCh'C
Cuts at: 173 359 376 556 778 816 952 1563 1656
Size: 186 17 180 222 38 136 611 93
Cuts at: 1656 1730 1920 1982 2712 173
Size: 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 222 190 186 180 136
93 74 62 38 17
BspEI T'CCGG_A
Cuts at: 3158 3158
Size: 3938
BspHI T'CATG_A
Cuts at: 3114 3114
Size: 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 806 988 1202 1504 1885 806
Size: 182 214 302 381 2859
Fragments arranged by size:
2859 381 302 214 182
BsrBI CCG'CTC
Cuts at: 50 2230 2284 3768 3933 50
Size: 2180 54 1484 165 55
Fragments arranged by size:
2180 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1850 1850
Size: 3938
BsrFI r'CCGG_y
Cuts at: 1936 2117 2326 1936
Size: 181 209 3548
Fragments arranged by size:
3548 209 181
BssSI C'ACGA_G
Cuts at: 2209 2567 2209
Size: 358 3580
BstAPI GCAn_nnn'nTGC
Cuts at: 504 560 644 1315 504
Size: 56 84 671 3127
Fragments arranged by size:
3127 671 84 56
BstBI TT'CG_AA
Cuts at: 2299 2299
Size: 3938
BstYI r'GATC_y
Cuts at: 1 963 1430 1453 1788 2034 3046 3132 3144
Size: 962 467 23 335 246 1012 86 12
Cuts at: 3144 3490 1
Size: 346 449
Fragments arranged by size:
1012 962 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 699 699
Size: 3938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1888 2069 2232 1888
Size: 181 163 3594
Fragments arranged by size:
3594 181 163
BtsI GCAGTG_nn'
Cuts at: 1181 1181
Size: 3938
DraI TTT'AAA
Cuts at: 937 937
Size: 3938
DrdI GACnn_nn'nnGTC
Cuts at: 1644 2502 1644
Size: 858 3080
EaeI y'GGCC_r
Cuts at: 1523 1697 2088 2115 3460 1523
Size: 174 391 27 1345 2001
Fragments arranged by size:
2001 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1523 1523
Size: 3938
EarI CTCTTCn'nnn_
Cuts at: 124 589 1961 2171 124
Size: 465 1372 210 1891
Fragments arranged by size:
1891 1372 465 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2146 2456 2602 3748 2146
Size: 310 146 1146 2336
Fragments arranged by size:
2336 1146 310 146
EcoICRI GAG'CTC
Cuts at: 171 950 171
Size: 779 3159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 613 697 709 1763 2195 2942 3210 323
Size: 290 84 12 1054 432 747 268 1051
Fragments arranged by size:
1054 1051 747 432 290 268 84 12
EcoNI CCTnn'n_nnAGG
Cuts at: 868 913 868
Size: 45 3893
EcoO109I rG'GnC_Cy
Cuts at: 372 373 775 789 1077 372
Size: 1 402 14 288 3233
Fragments arranged by size:
3233 402 288 14 1
EcoRI G'AATT_C
Cuts at: 942 942
Size: 3938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3938
FspI TGC'GCA
Cuts at: 1719 1719
Size: 3938
HaeII r_GCGC'y
Cuts at: 405 669 1372 1620 2642 3621 405
Size: 264 703 248 1022 979 722
Fragments arranged by size:
1022 979 722 703 264 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 407 439 1780 1810 1812 1842 407
Size: 32 1341 30 2 30 2503
Fragments arranged by size:
2503 1341 32 30 30 2
HincII GTy'rAC
Cuts at: 977 977
Size: 3938
KasI G'GCGC_C
Cuts at: 1368 1616 1368
Size: 248 3690
KpnI G_GTAC'C
Cuts at: 958 958
Size: 3938
MluI A'CGCG_T
Cuts at: 3371 3371
Size: 3938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2609 2793 2609
Size: 184 3754
MscI TGG'CCA
Cuts at: 1699 1699
Size: 3938
MslI CAynn'nnrTG
Cuts at: 205 467 1927 2054 205
Size: 262 1460 127 2089
Fragments arranged by size:
2089 1460 262 127
MspA1I CmG'CkG
Cuts at: 161 272 278 467 732 1049 1363 1723 2736
Size: 111 6 189 265 317 314 360 1013
Cuts at: 2736 2981 161
Size: 245 1118
Fragments arranged by size:
1118 1013 360 317 314 265 245 189
111 6
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 3938
NarI GG'CG_CC
Cuts at: 1369 1617 1369
Size: 248 3690
NcoI C'CATG_G
Cuts at: 699 699
Size: 3938
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 3938
NspI r_CATG'y
Cuts at: 507 991 1491 2398 507
Size: 484 500 907 2047
Fragments arranged by size:
2047 907 500 484
PciI A'CATG_T
Cuts at: 2394 2394
Size: 3938
PfoI T'CCnGG_A
Cuts at: 173 173
Size: 3938
PmeI GTTT'AAAC
Cuts at: 937 937
Size: 3938
PmlI CAC'GTG
Cuts at: 443 804 443
Size: 361 3577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3906
PpuMI rG'GwC_Cy
Cuts at: 789 789
Size: 3938
PspOMI G'GGCC_C
Cuts at: 372 774 372
Size: 402 3536
PstI C_TGCA'G
Cuts at: 500 872 985 1670 500
Size: 372 113 685 2768
Fragments arranged by size:
2768 685 372 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3938
PvuII CAG'CTG
Cuts at: 161 467 1363 1723 161
Size: 306 896 360 2376
Fragments arranged by size:
2376 896 360 306
RsrII CG'GwC_CG
Cuts at: 153 2133 153
Size: 1980 1958
SacI G_AGCT'C
Cuts at: 173 952 173
Size: 779 3159
SalI G'TCGA_C
Cuts at: 975 975
Size: 3938
SapI GCTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
SbfI CC_TGCA'GG
Cuts at: 985 985
Size: 3938
ScaI AGT'ACT
Cuts at: 3455 3455
Size: 3938
SexAI A'CCwGG_T
Cuts at: 533 533
Size: 3938
SfcI C'TryA_G
Cuts at: 33 496 868 981 1666 2659 2850 33
Size: 463 372 113 685 993 191 1121
Fragments arranged by size:
1121 993 685 463 372 191 113
SfoI GGC'GCC
Cuts at: 1370 1618 1370
Size: 248 3690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
SmaI CCC'GGG
Cuts at: 960 960
Size: 3938
SmlI C'TyrA_G
Cuts at: 520 1093 2008 2500 2762 520
Size: 573 915 492 262 1696
Fragments arranged by size:
1696 915 573 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3938
SphI G_CATG'C
Cuts at: 507 991 1491 507
Size: 484 500 2954
Fragments arranged by size:
2954 500 484
StyI C'CwwG_G
Cuts at: 213 699 1072 1426 213
Size: 486 373 354 2725
Fragments arranged by size:
2725 486 373 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 676 796 1521 1654 2113 2322 141
Size: 535 120 725 133 459 209 1757
Fragments arranged by size:
1757 725 535 459 209 133 120
TatI w'GTAC_w
Cuts at: 3453 3453
Size: 3938
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 3906
Tth111I GACn'n_nGTC
Cuts at: 1735 1735
Size: 3938
XbaI T'CTAG_A
Cuts at: 969 969
Size: 3938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 1433 120
Size: 1313 2625
XmaI C'CCGG_G
Cuts at: 958 958
Size: 3938
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI BaeI BbvCI
BfrBI BglI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI