Restriction map for
pF1KB7565
(Circular) (Six-base) MAPSORT of: pF1KB7565.seq Check: 7045 from: 1 to: 3740
pF1KB7565 3740 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 778 778
Size: 3740
Acc65I G'GTAC_C
Cuts at: 756 756
Size: 3740
AclI AA'CG_TT
Cuts at: 3580 3580
Size: 3740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1565 1997 2744 3012 1565
Size: 432 747 268 2293
Fragments arranged by size:
2293 747 432 268
AflIII A'CryG_T
Cuts at: 1092 2196 3173 1092
Size: 1104 977 1659
Fragments arranged by size:
1659 1104 977
AgeI A'CCGG_T
Cuts at: 2128 2128
Size: 3740
AlwNI CAG_nnn'CTG
Cuts at: 1045 2612 1045
Size: 1567 2173
ApaI G_GGCC'C
Cuts at: 631 631
Size: 3740
ApaLI G'TGCA_C
Cuts at: 2510 2510
Size: 3740
ApoI r'AATT_y
Cuts at: 226 744 3093 3349 226
Size: 518 2349 256 617
Fragments arranged by size:
2349 617 518 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3740
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
AvaI C'yCGr_G
Cuts at: 760 760
Size: 3740
BamHI G'GATC_C
Cuts at: 765 765
Size: 3740
BanI G'GyrC_C
Cuts at: 756 1170 1418 1453 756
Size: 414 248 35 3043
Fragments arranged by size:
3043 414 248 35
BanII G_rGCy'C
Cuts at: 631 754 1784 631
Size: 123 1030 2587
Fragments arranged by size:
2587 1030 123
BbeI G_GCGC'C
Cuts at: 1174 1422 1174
Size: 248 3492
BbsI GAAGACnn'nnnn_
Cuts at: 262 262
Size: 3740
BciVI GTATCCnnnnn_n'
Cuts at: 471 1638 2399 3740 471
Size: 1167 761 1341 471
Fragments arranged by size:
1341 1167 761 471
BclI T'GATC_A
Cuts at: 1260 1260
Size: 3740
BglII A'GATC_T
Cuts at: 1255 3292 1255
Size: 2037 1703
BlpI GC'TnA_GC
Cuts at: 554 852 554
Size: 298 3442
Bme1580I G_kGCm'C
Cuts at: 631 1365 1458 2514 631
Size: 734 93 1056 1857
Fragments arranged by size:
1857 1056 734 93
BmrI ACTGGGnnnn_n'
Cuts at: 952 1033 1368 3512 952
Size: 81 335 2144 1180
Fragments arranged by size:
2144 1180 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 3740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1795 2287 2585 916
Size: 879 492 298 2071
Fragments arranged by size:
2071 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 113 3444 113
Size: 3331 409
BsaAI yAC'GTr
Cuts at: 1093 1093
Size: 3740
BsaBI GATnn'nnATC
Cuts at: 6 1254 3291 6
Size: 1248 2037 455
Fragments arranged by size:
2037 1248 455
BsaHI Gr'CG_yC
Cuts at: 1171 1419 2121 3617 1171
Size: 248 702 1496 1294
Fragments arranged by size:
1496 1294 702 248
BsaWI w'CCGG_w
Cuts at: 929 1450 2128 2402 2549 2960 929
Size: 521 678 274 147 411 1709
Fragments arranged by size:
1709 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 691 694 697 700 724 691
Size: 3 3 3 24 3707
Fragments arranged by size:
3707 24 3 3 3
BseYI C'CCAG_C
Cuts at: 1165 1923 2500 2996 1165
Size: 758 577 496 1909
Fragments arranged by size:
1909 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 3740
BsiEI CG_ry'CG
Cuts at: 104 1282 1328 2536 104
Size: 1178 46 1208 1308
Fragments arranged by size:
1308 1208 1178 46
BsiHKAI G_wGCw'C
Cuts at: 188 754 1532 1722 2514 188
Size: 566 778 190 792 1414
Fragments arranged by size:
1414 792 778 566 190
BsiWI C'GTAC_G
Cuts at: 152 152
Size: 3740
Bsp1286I G_dGCh'C
Cuts at: 188 631 754 1365 1458 1532 1722 1784 2514
Size: 443 123 611 93 74 190 62 730
Cuts at: 2514 188
Size: 1414
Fragments arranged by size:
1414 730 611 443 190 123 93 74
62
BspEI T'CCGG_A
Cuts at: 2960 2960
Size: 3740
BspHI T'CATG_A
Cuts at: 2916 2916
Size: 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 181 790 1004 1306 1687 181
Size: 609 214 302 381 2234
Fragments arranged by size:
2234 609 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2032 2086 3570 3735 50
Size: 1982 54 1484 165 55
Fragments arranged by size:
1982 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1652 1652
Size: 3740
BsrFI r'CCGG_y
Cuts at: 1738 1919 2128 1738
Size: 181 209 3350
Fragments arranged by size:
3350 209 181
BssSI C'ACGA_G
Cuts at: 2011 2369 2011
Size: 358 3382
BstAPI GCAn_nnn'nTGC
Cuts at: 595 1117 595
Size: 522 3218
BstBI TT'CG_AA
Cuts at: 2101 2101
Size: 3740
BstYI r'GATC_y
Cuts at: 1 765 1232 1255 1590 1836 2848 2934 2946
Size: 764 467 23 335 246 1012 86 12
Cuts at: 2946 3292 1
Size: 346 449
Fragments arranged by size:
1012 764 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 133 107
Size: 26 3714
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 418 1690 1871 2034 418
Size: 1272 181 163 2124
Fragments arranged by size:
2124 1272 181 163
BtsI GCAGTG_nn'
Cuts at: 983 983
Size: 3740
DraI TTT'AAA
Cuts at: 739 739
Size: 3740
DrdI GACnn_nn'nnGTC
Cuts at: 211 1446 2304 211
Size: 1235 858 1647
Fragments arranged by size:
1647 1235 858
EaeI y'GGCC_r
Cuts at: 1325 1499 1890 1917 3262 1325
Size: 174 391 27 1345 1803
Fragments arranged by size:
1803 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1325 1325
Size: 3740
EarI CTCTTCn'nnn_
Cuts at: 176 213 710 1763 1973 176
Size: 37 497 1053 210 1943
Fragments arranged by size:
1943 1053 497 210 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1948 2258 2404 3550 1948
Size: 310 146 1146 2138
Fragments arranged by size:
2138 1146 310 146
EcoICRI GAG'CTC
Cuts at: 752 752
Size: 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1565 1997 2744 3012 1565
Size: 432 747 268 2293
Fragments arranged by size:
2293 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 350 350
Size: 3740
EcoO109I rG'GnC_Cy
Cuts at: 378 627 628 879 378
Size: 249 1 251 3239
Fragments arranged by size:
3239 251 249 1
EcoRI G'AATT_C
Cuts at: 226 744 226
Size: 518 3222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1759 1759
Size: 3740
FspI TGC'GCA
Cuts at: 1521 1521
Size: 3740
HaeII r_GCGC'y
Cuts at: 1174 1422 2444 3423 1174
Size: 248 1022 979 1491
Fragments arranged by size:
1491 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1582 1612 1614 1644 1582
Size: 30 2 30 3678
Fragments arranged by size:
3678 30 30 2
HincII GTy'rAC
Cuts at: 779 779
Size: 3740
KasI G'GCGC_C
Cuts at: 1170 1418 1170
Size: 248 3492
KpnI G_GTAC'C
Cuts at: 760 760
Size: 3740
MluI A'CGCG_T
Cuts at: 3173 3173
Size: 3740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2595 2411
Size: 184 3556
MscI TGG'CCA
Cuts at: 1501 1501
Size: 3740
MslI CAynn'nnrTG
Cuts at: 1729 1856 1729
Size: 127 3613
MspA1I CmG'CkG
Cuts at: 135 851 1165 1525 2538 2783 135
Size: 716 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 716 360 314 245
NaeI GCC'GGC
Cuts at: 1921 1921
Size: 3740
NarI GG'CG_CC
Cuts at: 1171 1419 1171
Size: 248 3492
NcoI C'CATG_G
Cuts at: 107 107
Size: 3740
NdeI CA'TA_TG
Cuts at: 537 537
Size: 3740
NgoMIV G'CCGG_C
Cuts at: 1919 1919
Size: 3740
NspI r_CATG'y
Cuts at: 793 1293 2200 793
Size: 500 907 2333
Fragments arranged by size:
2333 907 500
PciI A'CATG_T
Cuts at: 2196 2196
Size: 3740
PmeI GTTT'AAAC
Cuts at: 739 739
Size: 3740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3708
PpuMI rG'GwC_Cy
Cuts at: 378 378
Size: 3740
PspOMI G'GGCC_C
Cuts at: 627 627
Size: 3740
PstI C_TGCA'G
Cuts at: 787 1472 787
Size: 685 3055
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3740
PvuII CAG'CTG
Cuts at: 1165 1525 1165
Size: 360 3380
RsrII CG'GwC_CG
Cuts at: 1935 1935
Size: 3740
SacI G_AGCT'C
Cuts at: 754 754
Size: 3740
SacII CC_GC'GG
Cuts at: 136 136
Size: 3740
SalI G'TCGA_C
Cuts at: 777 777
Size: 3740
SapI GCTCTTCn'nnn_
Cuts at: 176 1763 1973 176
Size: 1587 210 1943
Fragments arranged by size:
1943 1587 210
SbfI CC_TGCA'GG
Cuts at: 787 787
Size: 3740
ScaI AGT'ACT
Cuts at: 3257 3257
Size: 3740
SfcI C'TryA_G
Cuts at: 33 783 1468 2461 2652 33
Size: 750 685 993 191 1121
Fragments arranged by size:
1121 993 750 685 191
SfoI GGC'GCC
Cuts at: 1172 1420 1172
Size: 248 3492
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
SmaI CCC'GGG
Cuts at: 762 762
Size: 3740
SmlI C'TyrA_G
Cuts at: 895 1810 2302 2564 895
Size: 915 492 262 2071
Fragments arranged by size:
2071 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3740
SphI G_CATG'C
Cuts at: 793 1293 793
Size: 500 3240
StyI C'CwwG_G
Cuts at: 107 599 874 1228 107
Size: 492 275 354 2619
Fragments arranged by size:
2619 492 354 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 1323 1456 1915 2124 484
Size: 839 133 459 209 2100
Fragments arranged by size:
2100 839 459 209 133
TatI w'GTAC_w
Cuts at: 3255 3255
Size: 3740
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 3708
Tth111I GACn'n_nGTC
Cuts at: 261 1537 261
Size: 1276 2464
XbaI T'CTAG_A
Cuts at: 771 771
Size: 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1235 1235
Size: 3740
XmaI C'CCGG_G
Cuts at: 760 760
Size: 3740
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI