Restriction map for
pF1KB7567
(Circular) (Six-base) MAPSORT of: pF1KB7567.seq Check: 2432 from: 1 to: 3743
pF1KB7567 3743 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 482 577 632 1568 2000 2747 3015 266
Size: 216 95 55 936 432 747 268 994
Fragments arranged by size:
994 936 747 432 268 216 95 55
AfeI AGC'GCT
Cuts at: 724 724
Size: 3743
AflIII A'CryG_T
Cuts at: 1095 2199 3176 1095
Size: 1104 977 1662
Fragments arranged by size:
1662 1104 977
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AlwNI CAG_nnn'CTG
Cuts at: 1048 2615 1048
Size: 1567 2176
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 747 3096 3352 747
Size: 2349 256 1138
Fragments arranged by size:
2349 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 473 763 473
Size: 290 3453
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 759 1173 1421 1456 759
Size: 414 248 35 3046
Fragments arranged by size:
3046 414 248 35
BanII G_rGCy'C
Cuts at: 131 317 570 757 1787 131
Size: 186 253 187 1030 2087
Fragments arranged by size:
2087 1030 253 187 186
BbeI G_GCGC'C
Cuts at: 1177 1425 1177
Size: 248 3495
BciVI GTATCCnnnnn_n'
Cuts at: 684 1641 2402 3743 684
Size: 957 761 1341 684
Fragments arranged by size:
1341 957 761 684
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglII A'GATC_T
Cuts at: 204 1258 3295 204
Size: 1054 2037 652
Fragments arranged by size:
2037 1054 652
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 1368 1461 2517 1368
Size: 93 1056 2594
Fragments arranged by size:
2594 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 283 955 1036 1371 3515 283
Size: 672 81 335 2144 511
Fragments arranged by size:
2144 672 511 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 585 205
Size: 380 3363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1798 2290 2588 919
Size: 879 492 298 2074
Fragments arranged by size:
2074 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 178 3447 178
Size: 3269 474
BsaAI yAC'GTr
Cuts at: 1096 1096
Size: 3743
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 1174 1422 2124 3620 1174
Size: 248 702 1496 1297
Fragments arranged by size:
1496 1297 702 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2405 2552 2963 932
Size: 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 265
Size: 3743
BseYI C'CCAG_C
Cuts at: 1168 1926 2503 2999 1168
Size: 758 577 496 1912
Fragments arranged by size:
1912 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 1285 1331 2539 104
Size: 12 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46 12
BsiHKAI G_wGCw'C
Cuts at: 131 317 570 757 1535 1725 2517 131
Size: 186 253 187 778 190 792 1357
Fragments arranged by size:
1357 792 778 253 190 187 186
BsmI GAATG_Cn'
Cuts at: 419 419
Size: 3743
BsmBI CGTCTCn'nnnn_
Cuts at: 112 112
Size: 3743
Bsp1286I G_dGCh'C
Cuts at: 131 317 570 757 1368 1461 1535 1725 1787
Size: 186 253 187 611 93 74 190 62
Cuts at: 1787 2517 131
Size: 730 1357
Fragments arranged by size:
1357 730 611 253 190 187 186 93
74 62
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 793 1007 1309 1690 793
Size: 214 302 381 2846
Fragments arranged by size:
2846 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1655 1655
Size: 3743
BsrFI r'CCGG_y
Cuts at: 1741 1922 2131 1741
Size: 181 209 3353
Fragments arranged by size:
3353 209 181
BssSI C'ACGA_G
Cuts at: 173 2014 2372 173
Size: 1841 358 1544
Fragments arranged by size:
1841 1544 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstYI r'GATC_y
Cuts at: 1 204 768 1235 1258 1593 1839 2851 2937
Size: 203 564 467 23 335 246 1012 86
Cuts at: 2937 2949 3295 1
Size: 12 346 449
Fragments arranged by size:
1012 564 467 449 346 335 246 203
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3743
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3399
Fragments arranged by size:
3399 181 163
BtsI GCAGTG_nn'
Cuts at: 986 986
Size: 3743
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2307 1449
Size: 858 2885
EaeI y'GGCC_r
Cuts at: 1328 1502 1893 1920 3265 1328
Size: 174 391 27 1345 1806
Fragments arranged by size:
1806 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 242 415 597 700 1766 1976 242
Size: 173 182 103 1066 210 2009
Fragments arranged by size:
2009 1066 210 182 173 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1951 2261 2407 3553 1951
Size: 310 146 1146 2141
Fragments arranged by size:
2141 1146 310 146
EcoICRI GAG'CTC
Cuts at: 129 315 568 755 129
Size: 186 253 187 3117
Fragments arranged by size:
3117 253 187 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 266 482 577 585 632 1568 2000 2747
Size: 61 216 95 8 47 936 432 747
Cuts at: 2747 3015 205
Size: 268 933
Fragments arranged by size:
936 933 747 432 268 216 95 61
47 8
EcoO109I rG'GnC_Cy
Cuts at: 882 882
Size: 3743
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 184 1762 184
Size: 1578 2165
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3743
HaeII r_GCGC'y
Cuts at: 726 1177 1425 2447 3426 726
Size: 451 248 1022 979 1043
Fragments arranged by size:
1043 1022 979 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1585 1615 1617 1647 1585
Size: 30 2 30 3681
Fragments arranged by size:
3681 30 30 2
HincII GTy'rAC
Cuts at: 543 782 543
Size: 239 3504
HindIII A'AGCT_T
Cuts at: 142 142
Size: 3743
HpaI GTT'AAC
Cuts at: 543 543
Size: 3743
KasI G'GCGC_C
Cuts at: 1173 1421 1173
Size: 248 3495
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 529 2414 2598 529
Size: 1885 184 1674
Fragments arranged by size:
1885 1674 184
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 1732 1859 1732
Size: 127 3616
MspA1I CmG'CkG
Cuts at: 670 854 1168 1528 2541 2786 670
Size: 184 314 360 1013 245 1627
Fragments arranged by size:
1627 1013 360 314 245 184
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3743
NarI GG'CG_CC
Cuts at: 1174 1422 1174
Size: 248 3495
NcoI C'CATG_G
Cuts at: 107 107
Size: 3743
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3743
NspI r_CATG'y
Cuts at: 244 796 1296 2203 244
Size: 552 500 907 1784
Fragments arranged by size:
1784 907 552 500
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3711
PstI C_TGCA'G
Cuts at: 790 1475 790
Size: 685 3058
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 670 1168 1528 670
Size: 498 360 2885
Fragments arranged by size:
2885 498 360
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 131 317 570 757 131
Size: 186 253 187 3117
Fragments arranged by size:
3117 253 187 186
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SfcI C'TryA_G
Cuts at: 33 786 1471 2464 2655 33
Size: 753 685 993 191 1121
Fragments arranged by size:
1121 993 753 685 191
SfoI GGC'GCC
Cuts at: 1175 1423 1175
Size: 248 3495
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 765 765
Size: 3743
SmlI C'TyrA_G
Cuts at: 898 1813 2305 2567 898
Size: 915 492 262 2074
Fragments arranged by size:
2074 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 796 1296 796
Size: 500 3243
StyI C'CwwG_G
Cuts at: 107 188 877 1231 107
Size: 81 689 354 2619
Fragments arranged by size:
2619 689 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 1326 1459 1918 2127 130
Size: 1196 133 459 209 1746
Fragments arranged by size:
1746 1196 459 209 133
TatI w'GTAC_w
Cuts at: 270 3258 270
Size: 2988 755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 3711
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3743
XmaI C'CCGG_G
Cuts at: 763 763
Size: 3743
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI