Restriction map for
pF1KB7569
(Circular) (Six-base) MAPSORT of: pF1KB7569.seq Check: 2796 from: 1 to: 3746
pF1KB7569 3746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AleI CACnn'nnGTG
Cuts at: 541 541
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 1051 2618 1051
Size: 1567 2179
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 181 189 280 766 181
Size: 8 91 486 3161
Fragments arranged by size:
3161 486 91 8
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 535 762 1176 1424 1459 535
Size: 227 414 248 35 2822
Fragments arranged by size:
2822 414 248 227 35
BanII G_rGCy'C
Cuts at: 281 760 1790 281
Size: 479 1030 2237
Fragments arranged by size:
2237 1030 479
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BbsI GAAGACnn'nnnn_
Cuts at: 329 329
Size: 3746
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 486 858 486
Size: 372 3374
Bme1580I G_kGCm'C
Cuts at: 182 1371 1464 2520 182
Size: 1189 93 1056 1408
Fragments arranged by size:
1408 1189 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 147 2113 147
Size: 1966 1780
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 427 1260 3297 6
Size: 421 833 2037 455
Fragments arranged by size:
2037 833 455 421
BsaHI Gr'CG_yC
Cuts at: 1177 1425 2127 3623 1177
Size: 248 702 1496 1300
Fragments arranged by size:
1496 1300 702 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 466
Size: 3746
BseYI C'CCAG_C
Cuts at: 1171 1929 2506 3002 1171
Size: 758 577 496 1915
Fragments arranged by size:
1915 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 173 1288 1334 2542 104
Size: 69 1115 46 1208 1308
Fragments arranged by size:
1308 1208 1115 69 46
BsiHKAI G_wGCw'C
Cuts at: 281 760 1538 1728 2520 281
Size: 479 778 190 792 1507
Fragments arranged by size:
1507 792 778 479 190
BsmBI CGTCTCn'nnnn_
Cuts at: 160 227 160
Size: 67 3679
Bsp1286I G_dGCh'C
Cuts at: 182 281 760 1371 1464 1538 1728 1790 2520
Size: 99 479 611 93 74 190 62 730
Cuts at: 2520 182
Size: 1408
Fragments arranged by size:
1408 730 611 479 190 99 93 74
62
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 485 2038 2092 3576 3741 50
Size: 435 1553 54 1484 165 55
Fragments arranged by size:
1553 1484 435 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 1744 1925 2134 1744
Size: 181 209 3356
Fragments arranged by size:
3356 209 181
BssSI C'ACGA_G
Cuts at: 121 2017 2375 121
Size: 1896 358 1492
Fragments arranged by size:
1896 1492 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 704 771 1238 1261 1596 1842 2854 2940
Size: 703 67 467 23 335 246 1012 86
Cuts at: 2940 2952 3298 1
Size: 12 346 449
Fragments arranged by size:
1012 703 467 449 346 335 246 86
67 23 12
Bsu36I CC'TnA_GG
Cuts at: 497 497
Size: 3746
BtgI C'CryG_G
Cuts at: 107 107
Size: 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 156 1452 2310 156
Size: 1296 858 1592
Fragments arranged by size:
1592 1296 858
EaeI y'GGCC_r
Cuts at: 170 1331 1505 1896 1923 3268 170
Size: 1161 174 391 27 1345 648
Fragments arranged by size:
1345 1161 648 391 174 27
EagI C'GGCC_G
Cuts at: 170 1331 170
Size: 1161 2585
EarI CTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2264 2410 3556 1954
Size: 310 146 1146 2144
Fragments arranged by size:
2144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 279 758 279
Size: 479 3267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 186 885 186
Size: 699 3047
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 1180 1428 2450 3429 1180
Size: 248 1022 979 1497
Fragments arranged by size:
1497 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 158 2417 2601 140
Size: 18 2259 184 1285
Fragments arranged by size:
2259 1285 184 18
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 541 1735 1862 541
Size: 1194 127 2425
Fragments arranged by size:
2425 1194 127
MspA1I CmG'CkG
Cuts at: 175 265 459 857 1171 1531 2544 2789 175
Size: 90 194 398 314 360 1013 245 1132
Fragments arranged by size:
1132 1013 398 360 314 245 194 90
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NcoI C'CATG_G
Cuts at: 107 107
Size: 3746
NdeI CA'TA_TG
Cuts at: 665 665
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PpuMI rG'GwC_Cy
Cuts at: 186 186
Size: 3746
PstI C_TGCA'G
Cuts at: 264 348 793 1478 264
Size: 84 445 685 2532
Fragments arranged by size:
2532 685 445 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 459 1171 1531 459
Size: 712 360 2674
Fragments arranged by size:
2674 712 360
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 281 760 281
Size: 479 3267
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SanDI GG'GwC_CC
Cuts at: 186 186
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 260 344 789 1474 2467 2658 33
Size: 227 84 445 685 993 191 1121
Fragments arranged by size:
1121 993 685 445 227 191 84
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 183 191 768 183
Size: 8 577 3161
Fragments arranged by size:
3161 577 8
SmlI C'TyrA_G
Cuts at: 280 901 1816 2308 2570 280
Size: 621 915 492 262 1456
Fragments arranged by size:
1456 915 621 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 581 880 1234 107
Size: 474 299 354 2619
Fragments arranged by size:
2619 474 354 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 529 1241 529
Size: 712 3034
XhoI C'TCGA_G
Cuts at: 280 280
Size: 3746
XmaI C'CCGG_G
Cuts at: 181 189 766 181
Size: 8 577 3161
Fragments arranged by size:
3161 577 8
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI