Restriction map for
pF1KB7571
(Circular) (Six-base) MAPSORT of: pF1KB7571.seq Check: 8048 from: 1 to: 3728
pF1KB7571 3728 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1553 1985 2732 3000 337
Size: 1216 432 747 268 1065
Fragments arranged by size:
1216 1065 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 553 691 1033 2600 553
Size: 138 342 1567 1681
Fragments arranged by size:
1681 1567 342 138
ApaI G_GGCC'C
Cuts at: 682 682
Size: 3728
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 224 232 675 748 224
Size: 8 443 73 3204
Fragments arranged by size:
3204 443 73 8
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 207 427 744 1158 1406 1441 207
Size: 220 317 414 248 35 2494
Fragments arranged by size:
2494 414 317 248 220 35
BanII G_rGCy'C
Cuts at: 239 622 682 742 1772 239
Size: 383 60 60 1030 2195
Fragments arranged by size:
2195 1030 383 60 60
BbeI G_GCGC'C
Cuts at: 431 1162 1410 431
Size: 731 248 2749
Fragments arranged by size:
2749 731 248
BbsI GAAGACnn'nnnn_
Cuts at: 301 407 301
Size: 106 3622
BbvCI CC'TCA_GC
Cuts at: 684 684
Size: 3728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3694
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 682 1353 1446 2502 682
Size: 671 93 1056 1908
Fragments arranged by size:
1908 1056 671 93
BmrI ACTGGGnnnn_n'
Cuts at: 273 940 1021 1356 3500 273
Size: 667 81 335 2144 501
Fragments arranged by size:
2144 667 501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 355
Size: 3728
Bpu10I CC'TnA_GC
Cuts at: 684 684
Size: 3728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 1081 1081
Size: 3728
BsaBI GATnn'nnATC
Cuts at: 6 1242 3279 6
Size: 1236 2037 455
Fragments arranged by size:
2037 1236 455
BsaHI Gr'CG_yC
Cuts at: 428 1159 1407 2109 3605 428
Size: 731 248 702 1496 551
Fragments arranged by size:
1496 731 702 551 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 328
Size: 30 3698
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 580
Size: 3728
BseYI C'CCAG_C
Cuts at: 666 1153 1911 2488 2984 666
Size: 487 758 577 496 1410
Fragments arranged by size:
1410 758 577 496 487
BsiEI CG_ry'CG
Cuts at: 104 462 1270 1316 2524 104
Size: 358 808 46 1208 1308
Fragments arranged by size:
1308 1208 808 358 46
BsiHKAI G_wGCw'C
Cuts at: 239 742 1520 1710 2502 239
Size: 503 778 190 792 1465
Fragments arranged by size:
1465 792 778 503 190
Bsp1286I G_dGCh'C
Cuts at: 239 622 682 742 1353 1446 1520 1710 1772
Size: 383 60 60 611 93 74 190 62
Cuts at: 1772 2502 239
Size: 730 1465
Fragments arranged by size:
1465 730 611 383 190 93 74 62
60 60
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 147 1726 1907 2116 147
Size: 1579 181 209 1759
Fragments arranged by size:
1759 1579 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstEII G'GTnAC_C
Cuts at: 273 273
Size: 3728
BstXI CCAn_nnnn'nTGG
Cuts at: 415 415
Size: 3728
BstYI r'GATC_y
Cuts at: 1 753 1220 1243 1578 1824 2836 2922 2934
Size: 752 467 23 335 246 1012 86 12
Cuts at: 2934 3280 1
Size: 346 449
Fragments arranged by size:
1012 752 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 328 971 328
Size: 643 3085
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 459 1313 1487 1878 1905 3250 459
Size: 854 174 391 27 1345 937
Fragments arranged by size:
1345 937 854 391 174 27
EagI C'GGCC_G
Cuts at: 459 1313 459
Size: 854 2874
EarI CTCTTCn'nnn_
Cuts at: 176 244 557 692 1751 1961 176
Size: 68 313 135 1059 210 1943
Fragments arranged by size:
1943 1059 313 210 135 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 237 740 237
Size: 503 3225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 355 1553 1985 2732 3000 337
Size: 18 1198 432 747 268 1065
Fragments arranged by size:
1198 1065 747 432 268 18
EcoO109I rG'GnC_Cy
Cuts at: 360 679 867 360
Size: 319 188 3221
Fragments arranged by size:
3221 319 188
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 232 431 673 1162 1410 2432 3411 232
Size: 199 242 489 248 1022 979 549
Fragments arranged by size:
1022 979 549 489 248 242 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 556 588 703 735 1570 1600 1602
Size: 32 4 32 115 32 835 30 2
Cuts at: 1602 1632 520
Size: 30 2616
Fragments arranged by size:
2616 835 115 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 767 767
Size: 3728
KasI G'GCGC_C
Cuts at: 427 1158 1406 427
Size: 731 248 2749
Fragments arranged by size:
2749 731 248
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2583 2399
Size: 184 3544
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 413 1717 1844 413
Size: 1304 127 2297
Fragments arranged by size:
2297 1304 127
MspA1I CmG'CkG
Cuts at: 455 839 1153 1513 2526 2771 455
Size: 384 314 360 1013 245 1412
Fragments arranged by size:
1412 1013 384 360 314 245
NaeI GCC'GGC
Cuts at: 149 1909 149
Size: 1760 1968
NarI GG'CG_CC
Cuts at: 428 1159 1407 428
Size: 731 248 2749
Fragments arranged by size:
2749 731 248
NgoMIV G'CCGG_C
Cuts at: 147 1907 147
Size: 1760 1968
NspI r_CATG'y
Cuts at: 387 781 1281 2188 387
Size: 394 500 907 1927
Fragments arranged by size:
1927 907 500 394
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PfoI T'CCnGG_A
Cuts at: 508 508
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PpuMI rG'GwC_Cy
Cuts at: 360 360
Size: 3728
PspOMI G'GGCC_C
Cuts at: 678 678
Size: 3728
PspXI vC'TCGA_Gb
Cuts at: 232 232
Size: 3728
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 1153 1513 1153
Size: 360 3368
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 239 742 239
Size: 503 3225
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 244 1751 1961 244
Size: 1507 210 2011
Fragments arranged by size:
2011 1507 210
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SexAI A'CCwGG_T
Cuts at: 362 362
Size: 3728
SfcI C'TryA_G
Cuts at: 33 771 1456 2449 2640 33
Size: 738 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 191
SfoI GGC'GCC
Cuts at: 429 1160 1408 429
Size: 731 248 2749
Fragments arranged by size:
2749 731 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SgrAI Cr'CCGG_yG
Cuts at: 147 147
Size: 3728
SmaI CCC'GGG
Cuts at: 226 677 750 226
Size: 451 73 3204
Fragments arranged by size:
3204 451 73
SmlI C'TyrA_G
Cuts at: 232 883 1798 2290 2552 232
Size: 651 915 492 262 1408
Fragments arranged by size:
1408 915 651 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
StuI AGG'CCT
Cuts at: 265 265
Size: 3728
StyI C'CwwG_G
Cuts at: 862 1216 862
Size: 354 3374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1311 1444 1903 2112 1311
Size: 133 459 209 2927
Fragments arranged by size:
2927 459 209 133
TatI w'GTAC_w
Cuts at: 128 3243 128
Size: 3115 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 456 488 3245 3277 456
Size: 32 2757 32 907
Fragments arranged by size:
2757 907 32 32
Tth111I GACn'n_nGTC
Cuts at: 279 1525 279
Size: 1246 2482
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XhoI C'TCGA_G
Cuts at: 232 232
Size: 3728
XmaI C'CCGG_G
Cuts at: 224 675 748 224
Size: 451 73 3204
Fragments arranged by size:
3204 451 73
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PsrI SacII
SanDI SfiI SnaBI SrfI SspI SwaI XmnI ZraI