Restriction map for
pF1KB7581
(Circular) (Six-base) MAPSORT of: pF1KB7581.seq Check: 499 from: 1 to: 3638
pF1KB7581 3638 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1463 1895 2642 2910 229
Size: 1234 432 747 268 957
Fragments arranged by size:
1234 957 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AleI CACnn'nnGTG
Cuts at: 229 229
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 143 252 943 2510 143
Size: 109 691 1567 1271
Fragments arranged by size:
1567 1271 691 109
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 658 658
Size: 3638
BamHI G'GATC_C
Cuts at: 604 663 604
Size: 59 3579
BanI G'GyrC_C
Cuts at: 654 1068 1316 1351 654
Size: 414 248 35 2941
Fragments arranged by size:
2941 414 248 35
BanII G_rGCy'C
Cuts at: 589 618 652 1682 589
Size: 29 34 1030 2545
Fragments arranged by size:
2545 1030 34 29
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BbsI GAAGACnn'nnnn_
Cuts at: 164 164
Size: 3638
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 1153 3190 1153
Size: 2037 1601
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 1263 1356 2412 1263
Size: 93 1056 2489
Fragments arranged by size:
2489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 584 850 931 1266 3410 584
Size: 266 81 335 2144 812
Fragments arranged by size:
2144 812 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 399 2005 399
Size: 1606 2032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 632
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1693 2185 2483 814
Size: 879 492 298 1969
Fragments arranged by size:
1969 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 547 3342 547
Size: 2795 843
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 1069 1317 2019 3515 1069
Size: 248 702 1496 1192
Fragments arranged by size:
1496 1192 702 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 576 606 617 647 576
Size: 30 11 30 3567
Fragments arranged by size:
3567 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 412 208
Size: 204 3434
BseYI C'CCAG_C
Cuts at: 485 580 1063 1821 2398 2894 485
Size: 95 483 758 577 496 1229
Fragments arranged by size:
1229 758 577 496 483 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 531 513
Size: 18 3620
BsiEI CG_ry'CG
Cuts at: 104 1180 1226 2434 104
Size: 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 46
BsiHKAI G_wGCw'C
Cuts at: 652 1430 1620 2412 652
Size: 778 190 792 1878
Fragments arranged by size:
1878 792 778 190
BsmI GAATG_Cn'
Cuts at: 429 429
Size: 3638
Bsp1286I G_dGCh'C
Cuts at: 589 618 652 1263 1356 1430 1620 1682 2412
Size: 29 34 611 93 74 190 62 730
Cuts at: 2412 589
Size: 1815
Fragments arranged by size:
1815 730 611 190 93 74 62 34
29
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 262 1636 1817 2026 262
Size: 1374 181 209 1874
Fragments arranged by size:
1874 1374 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 1015 1015
Size: 3638
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstYI r'GATC_y
Cuts at: 1 604 663 1130 1153 1488 1734 2746 2832
Size: 603 59 467 23 335 246 1012 86
Cuts at: 2832 2844 3190 1
Size: 12 346 449
Fragments arranged by size:
1012 603 467 449 346 335 246 86
59 23 12
BtgI C'CryG_G
Cuts at: 107 227 107
Size: 120 3518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 334 1588 1769 1932 334
Size: 1254 181 163 2040
Fragments arranged by size:
2040 1254 181 163
BtsI GCAGTG_nn'
Cuts at: 881 881
Size: 3638
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 110 1223 1397 1788 1815 3160 110
Size: 1113 174 391 27 1345 588
Fragments arranged by size:
1345 1113 588 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 545 1661 1871 545
Size: 1116 210 2312
Fragments arranged by size:
2312 1116 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 632 1463 1895 2642 2910 229
Size: 403 831 432 747 268 957
Fragments arranged by size:
957 831 747 432 403 268
EcoO109I rG'GnC_Cy
Cuts at: 777 777
Size: 3638
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 1657
Size: 3638
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 128 565 1072 1320 2342 3321 128
Size: 437 507 248 1022 979 445
Fragments arranged by size:
1022 979 507 445 437 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 576 608 1480 1510 1512 1542 154
Size: 32 390 32 872 30 2 30 2250
Fragments arranged by size:
2250 872 390 32 32 30 30 2
HincII GTy'rAC
Cuts at: 677 677
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2493 2309
Size: 184 3454
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 229 1627 1754 229
Size: 1398 127 2113
Fragments arranged by size:
2113 1398 127
MspA1I CmG'CkG
Cuts at: 140 489 749 1063 1423 2436 2681 140
Size: 349 260 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 360 349 314 260 245
NaeI GCC'GGC
Cuts at: 264 1819 264
Size: 1555 2083
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 107 227 107
Size: 120 3518
NgoMIV G'CCGG_C
Cuts at: 262 1817 262
Size: 1555 2083
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PfoI T'CCnGG_A
Cuts at: 371 371
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PstI C_TGCA'G
Cuts at: 685 1370 685
Size: 685 2953
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 1063 1423 1063
Size: 360 3278
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 681 1366 2359 2550 33
Size: 648 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 191
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 793 1708 2200 2462 793
Size: 915 492 262 1969
Fragments arranged by size:
1969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StyI C'CwwG_G
Cuts at: 107 227 428 772 1126 107
Size: 120 201 344 354 2619
Fragments arranged by size:
2619 354 344 201 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1221 1354 1813 2022 1221
Size: 133 459 209 2837
Fragments arranged by size:
2837 459 209 133
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3606
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1133 1133
Size: 3638
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI