Restriction map for
pF1KB7584
(Circular) (Six-base) MAPSORT of: pF1KB7584.seq Check: 1840 from: 1 to: 3974
pF1KB7584 3974 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1012 1012
Size: 3974
Acc65I G'GTAC_C
Cuts at: 990 990
Size: 3974
AclI AA'CG_TT
Cuts at: 3814 3814
Size: 3974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1799 2231 2978 3246 1799
Size: 432 747 268 2527
Fragments arranged by size:
2527 747 432 268
AfeI AGC'GCT
Cuts at: 240 240
Size: 3974
AflIII A'CryG_T
Cuts at: 1326 2430 3407 1326
Size: 1104 977 1893
Fragments arranged by size:
1893 1104 977
AgeI A'CCGG_T
Cuts at: 2362 2362
Size: 3974
AhdI GACnn_n'nnGTC
Cuts at: 432 432
Size: 3974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 814 814
Size: 3974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 634 666 634
Size: 32 3942
AlwNI CAG_nnn'CTG
Cuts at: 414 567 876 1279 2846 414
Size: 153 309 403 1567 1542
Fragments arranged by size:
1567 1542 403 309 153
ApaI G_GGCC'C
Cuts at: 944 944
Size: 3974
ApaLI G'TGCA_C
Cuts at: 2744 2744
Size: 3974
ApoI r'AATT_y
Cuts at: 978 3327 3583 978
Size: 2349 256 1369
Fragments arranged by size:
2349 1369 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
AvaI C'yCGr_G
Cuts at: 205 261 577 937 994 205
Size: 56 316 360 57 3185
Fragments arranged by size:
3185 360 316 57 56
BamHI G'GATC_C
Cuts at: 999 999
Size: 3974
BanI G'GyrC_C
Cuts at: 684 990 1404 1652 1687 684
Size: 306 414 248 35 2971
Fragments arranged by size:
2971 414 306 248 35
BanII G_rGCy'C
Cuts at: 184 944 988 2018 184
Size: 760 44 1030 2140
Fragments arranged by size:
2140 1030 760 44
BbeI G_GCGC'C
Cuts at: 1408 1656 1408
Size: 248 3726
BbsI GAAGACnn'nnnn_
Cuts at: 203 203
Size: 3974
BbvCI CC'TCA_GC
Cuts at: 908 908
Size: 3974
BciVI GTATCCnnnnn_n'
Cuts at: 1872 2633 3974 1872
Size: 761 1341 1872
Fragments arranged by size:
1872 1341 761
BclI T'GATC_A
Cuts at: 1494 1494
Size: 3974
BglI GCCn_nnn'nGGC
Cuts at: 179 592 179
Size: 413 3561
BglII A'GATC_T
Cuts at: 1489 3526 1489
Size: 2037 1937
BlpI GC'TnA_GC
Cuts at: 133 1086 133
Size: 953 3021
Bme1580I G_kGCm'C
Cuts at: 944 1599 1692 2748 944
Size: 655 93 1056 2170
Fragments arranged by size:
2170 1056 655 93
BmrI ACTGGGnnnn_n'
Cuts at: 424 555 1186 1267 1602 3746 424
Size: 131 631 81 335 2144 652
Fragments arranged by size:
2144 652 631 335 131 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 3974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 501 712 141
Size: 360 211 3403
Fragments arranged by size:
3403 360 211
Bpu10I CC'TnA_GC
Cuts at: 166 908 166
Size: 742 3232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 2029 2521 2819 1150
Size: 879 492 298 2305
Fragments arranged by size:
2305 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 3974
BsaAI yAC'GTr
Cuts at: 1327 1327
Size: 3974
BsaBI GATnn'nnATC
Cuts at: 6 1488 3525 6
Size: 1482 2037 455
Fragments arranged by size:
2037 1482 455
BsaHI Gr'CG_yC
Cuts at: 1405 1653 2355 3851 1405
Size: 248 702 1496 1528
Fragments arranged by size:
1528 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1163 1684 2362 2636 2783 3194 1163
Size: 521 678 274 147 411 1943
Fragments arranged by size:
1943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 808 838 361
Size: 30 417 30 3497
Fragments arranged by size:
3497 417 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 460
Size: 3974
BseYI C'CCAG_C
Cuts at: 245 603 829 1399 2157 2734 3230 245
Size: 358 226 570 758 577 496 989
Fragments arranged by size:
989 758 577 570 496 358 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 3974
BsiEI CG_ry'CG
Cuts at: 104 126 1516 1562 2770 104
Size: 22 1390 46 1208 1308
Fragments arranged by size:
1390 1308 1208 46 22
BsiHKAI G_wGCw'C
Cuts at: 698 988 1766 1956 2748 698
Size: 290 778 190 792 1924
Fragments arranged by size:
1924 792 778 290 190
Bsp1286I G_dGCh'C
Cuts at: 184 698 944 988 1599 1692 1766 1956 2018
Size: 514 246 44 611 93 74 190 62
Cuts at: 2018 2748 184
Size: 730 1410
Fragments arranged by size:
1410 730 611 514 246 190 93 74
62 44
BspEI T'CCGG_A
Cuts at: 3194 3194
Size: 3974
BspHI T'CATG_A
Cuts at: 3150 3150
Size: 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1024 1238 1540 1921 1024
Size: 214 302 381 3077
Fragments arranged by size:
3077 381 302 214
BsrBI CCG'CTC
Cuts at: 50 810 2266 2320 3804 3969 50
Size: 760 1456 54 1484 165 55
Fragments arranged by size:
1484 1456 760 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 1886
Size: 3974
BsrFI r'CCGG_y
Cuts at: 383 1972 2153 2362 383
Size: 1589 181 209 1995
Fragments arranged by size:
1995 1589 209 181
BssHII G'CGCG_C
Cuts at: 582 582
Size: 3974
BssSI C'ACGA_G
Cuts at: 2245 2603 2245
Size: 358 3616
BstAPI GCAn_nnn'nTGC
Cuts at: 414 1351 414
Size: 937 3037
BstBI TT'CG_AA
Cuts at: 2335 2335
Size: 3974
BstXI CCAn_nnnn'nTGG
Cuts at: 274 837 274
Size: 563 3411
BstYI r'GATC_y
Cuts at: 1 999 1466 1489 1824 2070 3082 3168 3180
Size: 998 467 23 335 246 1012 86 12
Cuts at: 3180 3526 1
Size: 346 449
Fragments arranged by size:
1012 998 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 719 719
Size: 3974
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1924 2105 2268 1924
Size: 181 163 3630
Fragments arranged by size:
3630 181 163
BtsI GCAGTG_nn'
Cuts at: 1217 1217
Size: 3974
DraI TTT'AAA
Cuts at: 973 973
Size: 3974
DrdI GACnn_nn'nnGTC
Cuts at: 1680 2538 1680
Size: 858 3116
EaeI y'GGCC_r
Cuts at: 276 530 593 1559 1733 2124 2151 3496 276
Size: 254 63 966 174 391 27 1345 754
Fragments arranged by size:
1345 966 754 391 254 174 63 27
EagI C'GGCC_G
Cuts at: 1559 1559
Size: 3974
EarI CTCTTCn'nnn_
Cuts at: 768 1997 2207 768
Size: 1229 210 2535
Fragments arranged by size:
2535 1229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2182 2492 2638 3784 2182
Size: 310 146 1146 2372
Fragments arranged by size:
2372 1146 310 146
EcoICRI GAG'CTC
Cuts at: 986 986
Size: 3974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 501 712 1799 2231 2978 3246 141
Size: 360 211 1087 432 747 268 869
Fragments arranged by size:
1087 869 747 432 360 268 211
EcoO109I rG'GnC_Cy
Cuts at: 426 941 1113 426
Size: 515 172 3287
Fragments arranged by size:
3287 515 172
EcoRI G'AATT_C
Cuts at: 978 978
Size: 3974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3974
FspI TGC'GCA
Cuts at: 466 1755 466
Size: 1289 2685
FspAI rTGC'GCAy
Cuts at: 466 466
Size: 3974
HaeII r_GCGC'y
Cuts at: 242 344 546 574 1408 1656 2678 3657 242
Size: 102 202 28 834 248 1022 979 559
Fragments arranged by size:
1022 979 834 559 248 202 102 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 418 450 1816 1846 1848 1878 244
Size: 32 142 32 1366 30 2 30 2340
Fragments arranged by size:
2340 1366 142 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1013 1013
Size: 3974
KasI G'GCGC_C
Cuts at: 1404 1652 1404
Size: 248 3726
KpnI G_GTAC'C
Cuts at: 994 994
Size: 3974
MluI A'CGCG_T
Cuts at: 3407 3407
Size: 3974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 338 2645 2829 338
Size: 2307 184 1483
Fragments arranged by size:
2307 1483 184
MscI TGG'CCA
Cuts at: 278 1735 278
Size: 1457 2517
MslI CAynn'nnrTG
Cuts at: 1963 2090 1963
Size: 127 3847
MspA1I CmG'CkG
Cuts at: 414 528 603 690 798 1085 1399 1759 2772
Size: 114 75 87 108 287 314 360 1013
Cuts at: 2772 3017 414
Size: 245 1371
Fragments arranged by size:
1371 1013 360 314 287 245 114 108
87 75
NaeI GCC'GGC
Cuts at: 2155 2155
Size: 3974
NarI GG'CG_CC
Cuts at: 1405 1653 1405
Size: 248 3726
NcoI C'CATG_G
Cuts at: 719 719
Size: 3974
NgoMIV G'CCGG_C
Cuts at: 2153 2153
Size: 3974
NspI r_CATG'y
Cuts at: 1027 1527 2434 1027
Size: 500 907 2567
Fragments arranged by size:
2567 907 500
PasI CC'CwG_GG
Cuts at: 955 955
Size: 3974
PciI A'CATG_T
Cuts at: 2430 2430
Size: 3974
PflMI CCAn_nnn'nTGG
Cuts at: 567 857 567
Size: 290 3684
PmeI GTTT'AAAC
Cuts at: 973 973
Size: 3974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 3942
PpuMI rG'GwC_Cy
Cuts at: 426 426
Size: 3974
PspOMI G'GGCC_C
Cuts at: 940 940
Size: 3974
PstI C_TGCA'G
Cuts at: 542 1021 1706 542
Size: 479 685 2810
Fragments arranged by size:
2810 685 479
PvuI CG_AT'CG
Cuts at: 104 126 104
Size: 22 3952
PvuII CAG'CTG
Cuts at: 528 603 798 1399 1759 528
Size: 75 195 601 360 2743
Fragments arranged by size:
2743 601 360 195 75
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 3974
SacI G_AGCT'C
Cuts at: 988 988
Size: 3974
SalI G'TCGA_C
Cuts at: 1011 1011
Size: 3974
SapI GCTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
SbfI CC_TGCA'GG
Cuts at: 1021 1021
Size: 3974
ScaI AGT'ACT
Cuts at: 3491 3491
Size: 3974
SfcI C'TryA_G
Cuts at: 33 234 538 1017 1702 2695 2886 33
Size: 201 304 479 685 993 191 1121
Fragments arranged by size:
1121 993 685 479 304 201 191
SfoI GGC'GCC
Cuts at: 1406 1654 1406
Size: 248 3726
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
SmaI CCC'GGG
Cuts at: 939 996 939
Size: 57 3917
SmlI C'TyrA_G
Cuts at: 1129 2044 2536 2798 1129
Size: 915 492 262 2305
Fragments arranged by size:
2305 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3974
SphI G_CATG'C
Cuts at: 1027 1527 1027
Size: 500 3474
StyI C'CwwG_G
Cuts at: 185 719 1108 1462 185
Size: 534 389 354 2697
Fragments arranged by size:
2697 534 389 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1557 1690 2149 2358 1557
Size: 133 459 209 3173
Fragments arranged by size:
3173 459 209 133
TatI w'GTAC_w
Cuts at: 3489 3489
Size: 3974
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 3942
Tth111I GACn'n_nGTC
Cuts at: 1771 1771
Size: 3974
XbaI T'CTAG_A
Cuts at: 1005 1005
Size: 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 525 1469 525
Size: 944 3030
XmaI C'CCGG_G
Cuts at: 937 994 937
Size: 57 3917
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI