Restriction map for
pF1KB7587
(Circular) (Six-base) MAPSORT of: pF1KB7587.seq Check: 5931 from: 1 to: 3785
pF1KB7587 3785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 779 3625 779
Size: 2846 939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1610 2042 2789 3057 439
Size: 1171 432 747 268 1167
Fragments arranged by size:
1171 1167 747 432 268
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 303 453 510 1090 2657 303
Size: 150 57 580 1567 1431
Fragments arranged by size:
1567 1431 580 150 57
ApaI G_GGCC'C
Cuts at: 254 569 254
Size: 315 3470
ApaLI G'TGCA_C
Cuts at: 632 2555 632
Size: 1923 1862
ApoI r'AATT_y
Cuts at: 789 3138 3394 789
Size: 2349 256 1180
Fragments arranged by size:
2349 1180 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 238 280 805 238
Size: 42 525 3218
Fragments arranged by size:
3218 525 42
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 579 801 1215 1463 1498 579
Size: 222 414 248 35 2866
Fragments arranged by size:
2866 414 248 222 35
BanII G_rGCy'C
Cuts at: 227 238 254 569 799 1829 227
Size: 11 16 315 230 1030 2183
Fragments arranged by size:
2183 1030 315 230 16 11
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglI GCCn_nnn'nGGC
Cuts at: 412 412
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 514 897 514
Size: 383 3402
Bme1580I G_kGCm'C
Cuts at: 254 569 584 636 1410 1503 2559 254
Size: 315 15 52 774 93 1056 1480
Fragments arranged by size:
1480 1056 774 315 93 52 15
BmrI ACTGGGnnnn_n'
Cuts at: 997 1078 1413 3557 997
Size: 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 3785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 487 658 172
Size: 315 171 3299
Fragments arranged by size:
3299 315 171
Bpu10I CC'TnA_GC
Cuts at: 232 232
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 373 961 1840 2332 2630 277
Size: 96 588 879 492 298 1432
Fragments arranged by size:
1432 879 588 492 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 1216 1464 2166 3662 1216
Size: 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 592 622 688 718 592
Size: 30 66 30 3659
Fragments arranged by size:
3659 66 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 547
Size: 3785
BseYI C'CCAG_C
Cuts at: 270 569 583 1210 1968 2545 3041 270
Size: 299 14 627 758 577 496 1014
Fragments arranged by size:
1014 758 627 577 496 299 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 685
Size: 3785
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 227 287 636 799 1577 1767 2559 227
Size: 60 349 163 778 190 792 1453
Fragments arranged by size:
1453 792 778 349 190 163 60
BsmI GAATG_Cn'
Cuts at: 595 595
Size: 3785
Bsp1286I G_dGCh'C
Cuts at: 227 238 254 287 569 584 636 799 1410
Size: 11 16 33 282 15 52 163 611
Cuts at: 1410 1503 1577 1767 1829 2559 227
Size: 93 74 190 62 730 1453
Fragments arranged by size:
1453 730 611 282 190 163 93 74
62 52 33 16 15 11
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 707 835 1049 1351 1732 182
Size: 525 128 214 302 381 2235
Fragments arranged by size:
2235 525 381 302 214 128
BsrBI CCG'CTC
Cuts at: 50 216 2077 2131 3615 3780 50
Size: 166 1861 54 1484 165 55
Fragments arranged by size:
1861 1484 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 350 1783 1964 2173 350
Size: 1433 181 209 1962
Fragments arranged by size:
1962 1433 209 181
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 197 2146 197
Size: 1949 1836
BstYI r'GATC_y
Cuts at: 1 467 810 1277 1300 1635 1881 2893 2979
Size: 466 343 467 23 335 246 1012 86
Cuts at: 2979 2991 3337 1
Size: 12 346 449
Fragments arranged by size:
1012 467 466 449 346 343 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 307 344 107
Size: 200 37 3548
Fragments arranged by size:
3548 200 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 483 636 1028 483
Size: 153 392 3240
Fragments arranged by size:
3240 392 153
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 362 1370 1544 1935 1962 3307 362
Size: 1008 174 391 27 1345 840
Fragments arranged by size:
1345 1008 840 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 415 1808 2018 415
Size: 1393 210 2182
Fragments arranged by size:
2182 1393 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 225 797 225
Size: 572 3213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 439 487 658 1610 2042 2789 3057 172
Size: 267 48 171 952 432 747 268 900
Fragments arranged by size:
952 900 747 432 268 267 171 48
EcoNI CCTnn'n_nnAGG
Cuts at: 526 526
Size: 3785
EcoO109I rG'GnC_Cy
Cuts at: 243 250 251 275 507 566 924 243
Size: 7 1 24 232 59 358 3104
Fragments arranged by size:
3104 358 232 59 24 7 1
EcoRI G'AATT_C
Cuts at: 789 789
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 475 1566 475
Size: 1091 2694
HaeII r_GCGC'y
Cuts at: 501 608 650 1219 1467 2489 3468 501
Size: 107 42 569 248 1022 979 818
Fragments arranged by size:
1022 979 818 569 248 107 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1627 1657 1659 1689 1627
Size: 30 2 30 3723
Fragments arranged by size:
3723 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2456 2640 2456
Size: 184 3601
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 733 1774 1901 733
Size: 1041 127 2617
Fragments arranged by size:
2617 1041 127
MspA1I CmG'CkG
Cuts at: 450 896 1210 1570 2583 2828 450
Size: 446 314 360 1013 245 1407
Fragments arranged by size:
1407 1013 446 360 314 245
NaeI GCC'GGC
Cuts at: 1966 1966
Size: 3785
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NcoI C'CATG_G
Cuts at: 107 107
Size: 3785
NgoMIV G'CCGG_C
Cuts at: 1964 1964
Size: 3785
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PasI CC'CwG_GG
Cuts at: 247 655 247
Size: 408 3377
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PpuMI rG'GwC_Cy
Cuts at: 275 275
Size: 3785
PspOMI G'GGCC_C
Cuts at: 250 565 250
Size: 315 3470
PstI C_TGCA'G
Cuts at: 616 832 1517 616
Size: 216 685 2884
Fragments arranged by size:
2884 685 216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 227 799 227
Size: 572 3213
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SanDI GG'GwC_CC
Cuts at: 275 275
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 612 683 828 1513 2506 2697 33
Size: 579 71 145 685 993 191 1121
Fragments arranged by size:
1121 993 685 579 191 145 71
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 256 388 940 1855 2347 2609 256
Size: 132 552 915 492 262 1432
Fragments arranged by size:
1432 915 552 492 262 132
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StuI AGG'CCT
Cuts at: 406 406
Size: 3785
StyI C'CwwG_G
Cuts at: 107 458 919 1273 107
Size: 351 461 354 2619
Fragments arranged by size:
2619 461 354 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 488 3300 488
Size: 2812 973
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 3302 3334 592
Size: 32 2678 32 1043
Fragments arranged by size:
2678 1043 32 32
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI