Restriction map for
pF1KB7590
(Circular) (Six-base) MAPSORT of: pF1KB7590.seq Check: 153 from: 1 to: 3983
pF1KB7590 3983 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2987 3255 1808
Size: 432 747 268 2536
Fragments arranged by size:
2536 747 432 268
AfeI AGC'GCT
Cuts at: 475 475
Size: 3983
AflIII A'CryG_T
Cuts at: 421 1335 2439 3416 421
Size: 914 1104 977 988
Fragments arranged by size:
1104 988 977 914
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 348 364 348
Size: 16 3967
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 451 483 451
Size: 32 3951
AlwNI CAG_nnn'CTG
Cuts at: 959 1288 2855 959
Size: 329 1567 2087
Fragments arranged by size:
2087 1567 329
ApaI G_GGCC'C
Cuts at: 142 317 699 142
Size: 175 382 3426
Fragments arranged by size:
3426 382 175
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 987 3336 3592 987
Size: 2349 256 1378
Fragments arranged by size:
2349 1378 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 310 1003 310
Size: 693 3290
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 121 754 999 1413 1661 1696 121
Size: 633 245 414 248 35 2408
Fragments arranged by size:
2408 633 414 248 245 35
BanII G_rGCy'C
Cuts at: 142 317 699 997 2027 142
Size: 175 382 298 1030 2098
Fragments arranged by size:
2098 1030 382 298 175
BbeI G_GCGC'C
Cuts at: 125 1417 1665 125
Size: 1292 248 2443
Fragments arranged by size:
2443 1292 248
BbvCI CC'TCA_GC
Cuts at: 255 621 813 255
Size: 366 192 3425
Fragments arranged by size:
3425 366 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 619 653 619
Size: 34 3949
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglI GCCn_nnn'nGGC
Cuts at: 631 731 752 631
Size: 100 21 3862
Fragments arranged by size:
3862 100 21
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 142 317 692 699 757 1608 1701 2757 142
Size: 175 375 7 58 851 93 1056 1368
Fragments arranged by size:
1368 1056 851 375 175 93 58 7
BmrI ACTGGGnnnn_n'
Cuts at: 907 1195 1276 1611 3755 907
Size: 288 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 288 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 261 478 622 685 246
Size: 15 217 144 63 3544
Fragments arranged by size:
3544 217 144 63 15
Bpu10I CC'TnA_GC
Cuts at: 255 621 813 255
Size: 366 192 3425
Fragments arranged by size:
3425 366 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2530 2828 1159
Size: 879 492 298 2314
Fragments arranged by size:
2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 179 529 590 3687 179
Size: 350 61 3097 475
Fragments arranged by size:
3097 475 350 61
BsaAI yAC'GTr
Cuts at: 424 1336 424
Size: 912 3071
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 122 613 1414 1662 2364 3860 122
Size: 491 801 248 702 1496 245
Fragments arranged by size:
1496 801 702 491 248 245
BsaWI w'CCGG_w
Cuts at: 1172 1693 2371 2645 2792 3203 1172
Size: 521 678 274 147 411 1952
Fragments arranged by size:
1952 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 451 481 451
Size: 30 3953
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 609 771 609
Size: 162 3821
BseYI C'CCAG_C
Cuts at: 1408 2166 2743 3239 1408
Size: 758 577 496 2152
Fragments arranged by size:
2152 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 135 1525 1571 2779 104
Size: 31 1390 46 1208 1308
Fragments arranged by size:
1390 1308 1208 46 31
BsiHKAI G_wGCw'C
Cuts at: 997 1775 1965 2757 997
Size: 778 190 792 2223
Fragments arranged by size:
2223 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 142 317 692 699 757 997 1608 1701 1775
Size: 175 375 7 58 240 611 93 74
Cuts at: 1775 1965 2027 2757 142
Size: 190 62 730 1368
Fragments arranged by size:
1368 730 611 375 240 190 175 93
74 62 58 7
BspEI T'CCGG_A
Cuts at: 3203 3203
Size: 3983
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 747 1033 1247 1549 1930 747
Size: 286 214 302 381 2800
Fragments arranged by size:
2800 381 302 286 214
BsrBI CCG'CTC
Cuts at: 50 199 870 2275 2329 3813 3978 50
Size: 149 671 1405 54 1484 165 55
Fragments arranged by size:
1484 1405 671 165 149 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 407 674 886 1981 2162 2371 407
Size: 267 212 1095 181 209 2019
Fragments arranged by size:
2019 1095 267 212 209 181
BssHII G'CGCG_C
Cuts at: 145 145
Size: 3983
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstEII G'GTnAC_C
Cuts at: 766 766
Size: 3983
BstXI CCAn_nnnn'nTGG
Cuts at: 926 926
Size: 3983
BstYI r'GATC_y
Cuts at: 1 876 1008 1475 1498 1833 2079 3091 3177
Size: 875 132 467 23 335 246 1012 86
Cuts at: 3177 3189 3535 1
Size: 12 346 449
Fragments arranged by size:
1012 875 467 449 346 335 246 132
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 389 762 389
Size: 373 3610
BtgI C'CryG_G
Cuts at: 107 107
Size: 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 128 1933 2114 2277 128
Size: 1805 181 163 1834
Fragments arranged by size:
1834 1805 181 163
BtsI GCAGTG_nn'
Cuts at: 330 434 826 1226 330
Size: 104 392 400 3087
Fragments arranged by size:
3087 400 392 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 224 259 224
Size: 35 3948
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 225 245 1568 1742 2133 2160 3505 225
Size: 20 1323 174 391 27 1345 703
Fragments arranged by size:
1345 1323 703 391 174 27 20
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 169 2191 2501 2647 3793 169
Size: 2022 310 146 1146 359
Fragments arranged by size:
2022 1146 359 310 146
EcoICRI GAG'CTC
Cuts at: 995 995
Size: 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 261 478 622 685 1808 2240 2987 3255
Size: 15 217 144 63 1123 432 747 268
Cuts at: 3255 246
Size: 974
Fragments arranged by size:
1123 974 747 432 268 217 144 63
15
EcoNI CCTnn'n_nnAGG
Cuts at: 388 388
Size: 3983
EcoO109I rG'GnC_Cy
Cuts at: 138 139 251 393 695 696 1122 138
Size: 1 112 142 302 1 426 2999
Fragments arranged by size:
2999 426 302 142 112 1 1
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 125 180 477 715 953 1417 1665 2687 3666
Size: 55 297 238 238 464 248 1022 979
Cuts at: 3666 125
Size: 442
Fragments arranged by size:
1022 979 464 442 297 248 238 238
55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1825 1855 1857 1887 1825
Size: 30 2 30 3921
Fragments arranged by size:
3921 30 30 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
KasI G'GCGC_C
Cuts at: 121 1413 1661 121
Size: 1292 248 2443
Fragments arranged by size:
2443 1292 248
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 671 2654 2838 671
Size: 1983 184 1816
Fragments arranged by size:
1983 1816 184
MscI TGG'CCA
Cuts at: 227 1744 227
Size: 1517 2466
MslI CAynn'nnrTG
Cuts at: 346 1972 2099 346
Size: 1626 127 2230
Fragments arranged by size:
2230 1626 127
MspA1I CmG'CkG
Cuts at: 194 447 522 801 847 926 959 1094 1408
Size: 253 75 279 46 79 33 135 314
Cuts at: 1408 1768 2781 3026 194
Size: 360 1013 245 1151
Fragments arranged by size:
1151 1013 360 314 279 253 245 135
79 75 46 33
NaeI GCC'GGC
Cuts at: 676 888 2164 676
Size: 212 1276 2495
Fragments arranged by size:
2495 1276 212
NarI GG'CG_CC
Cuts at: 122 1414 1662 122
Size: 1292 248 2443
Fragments arranged by size:
2443 1292 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 674 886 2162 674
Size: 212 1276 2495
Fragments arranged by size:
2495 1276 212
NspI r_CATG'y
Cuts at: 1036 1536 2443 1036
Size: 500 907 2576
Fragments arranged by size:
2576 907 500
PasI CC'CwG_GG
Cuts at: 692 692
Size: 3983
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PflMI CCAn_nnn'nTGG
Cuts at: 486 486
Size: 3983
PfoI T'CCnGG_A
Cuts at: 872 872
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 483 3122 3154 451
Size: 32 2639 32 1280
Fragments arranged by size:
2639 1280 32 32
PpuMI rG'GwC_Cy
Cuts at: 251 251
Size: 3983
PspOMI G'GGCC_C
Cuts at: 138 313 695 138
Size: 175 382 3426
Fragments arranged by size:
3426 382 175
PstI C_TGCA'G
Cuts at: 1030 1715 1030
Size: 685 3298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 447 522 847 926 959 1408 1768 447
Size: 75 325 79 33 449 360 2662
Fragments arranged by size:
2662 449 360 325 79 75 33
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 997 997
Size: 3983
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 1026 1711 2704 2895 33
Size: 993 685 993 191 1121
Fragments arranged by size:
1121 993 993 685 191
SfoI GGC'GCC
Cuts at: 123 1415 1663 123
Size: 1292 248 2443
Fragments arranged by size:
2443 1292 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 312 1005 312
Size: 693 3290
SmlI C'TyrA_G
Cuts at: 1138 2053 2545 2807 1138
Size: 915 492 262 2314
Fragments arranged by size:
2314 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
SrfI GCCC'GGGC
Cuts at: 312 312
Size: 3983
StuI AGG'CCT
Cuts at: 634 734 634
Size: 100 3883
StyI C'CwwG_G
Cuts at: 107 1117 1471 107
Size: 1010 354 2619
Fragments arranged by size:
2619 1010 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1566 1699 2158 2367 1566
Size: 133 459 209 3182
Fragments arranged by size:
3182 459 209 133
TatI w'GTAC_w
Cuts at: 3498 3498
Size: 3983
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 310 1003 310
Size: 693 3290
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI