Restriction map for
pF1KB7592
(Circular) (Six-base) MAPSORT of: pF1KB7592.seq Check: 6452 from: 1 to: 3986
pF1KB7592 3986 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1024 1024
Size: 3986
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 180 3826 180
Size: 3646 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 970 1811 2243 2990 3258 838
Size: 132 841 432 747 268 1566
Fragments arranged by size:
1566 841 747 432 268 132
AflIII A'CryG_T
Cuts at: 1338 2442 3419 1338
Size: 1104 977 1905
Fragments arranged by size:
1905 1104 977
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 762 1291 2858 762
Size: 529 1567 1890
Fragments arranged by size:
1890 1567 529
ApaLI G'TGCA_C
Cuts at: 2756 2756
Size: 3986
ApoI r'AATT_y
Cuts at: 267 410 602 751 990 3339 3595 267
Size: 143 192 149 239 2349 256 658
Fragments arranged by size:
2349 658 256 239 192 149 143
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 1006 1006
Size: 3986
BamHI G'GATC_C
Cuts at: 111 1011 111
Size: 900 3086
BanI G'GyrC_C
Cuts at: 1002 1416 1664 1699 1002
Size: 414 248 35 3289
Fragments arranged by size:
3289 414 248 35
BanII G_rGCy'C
Cuts at: 156 1000 2030 156
Size: 844 1030 2112
Fragments arranged by size:
2112 1030 844
BbeI G_GCGC'C
Cuts at: 1420 1668 1420
Size: 248 3738
BbsI GAAGACnn'nnnn_
Cuts at: 370 620 370
Size: 250 3736
BciVI GTATCCnnnnn_n'
Cuts at: 1884 2645 3986 1884
Size: 761 1341 1884
Fragments arranged by size:
1884 1341 761
BclI T'GATC_A
Cuts at: 273 1506 273
Size: 1233 2753
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 1098 1098
Size: 3986
Bme1580I G_kGCm'C
Cuts at: 1611 1704 2760 1611
Size: 93 1056 2837
Fragments arranged by size:
2837 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1198 1279 1614 3758 1198
Size: 81 335 2144 1426
Fragments arranged by size:
2144 1426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 3986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 962
Size: 3986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1162 2041 2533 2831 430
Size: 732 879 492 298 1585
Fragments arranged by size:
1585 879 732 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3690 3690
Size: 3986
BsaAI yAC'GTr
Cuts at: 1339 1339
Size: 3986
BsaBI GATnn'nnATC
Cuts at: 6 389 1500 3537 6
Size: 383 1111 2037 455
Fragments arranged by size:
2037 1111 455 383
BsaHI Gr'CG_yC
Cuts at: 1417 1665 2367 3863 1417
Size: 248 702 1496 1540
Fragments arranged by size:
1540 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1175 1696 2374 2648 2795 3206 1175
Size: 521 678 274 147 411 1955
Fragments arranged by size:
1955 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 395 783 811 395
Size: 388 28 3570
Fragments arranged by size:
3570 388 28
BseYI C'CCAG_C
Cuts at: 1411 2169 2746 3242 1411
Size: 758 577 496 2155
Fragments arranged by size:
2155 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1528 1574 2782 104
Size: 1424 46 1208 1308
Fragments arranged by size:
1424 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 156 1000 1778 1968 2760 156
Size: 844 778 190 792 1382
Fragments arranged by size:
1382 844 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 156 1000 1611 1704 1778 1968 2030 2760 156
Size: 844 611 93 74 190 62 730 1382
Fragments arranged by size:
1382 844 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3206 3206
Size: 3986
BspHI T'CATG_A
Cuts at: 3162 3162
Size: 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1250 1552 1933 1036
Size: 214 302 381 3089
Fragments arranged by size:
3089 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2278 2332 3816 3981 50
Size: 2228 54 1484 165 55
Fragments arranged by size:
2228 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 288 1898 288
Size: 1610 2376
BsrFI r'CCGG_y
Cuts at: 1984 2165 2374 1984
Size: 181 209 3596
Fragments arranged by size:
3596 209 181
BssSI C'ACGA_G
Cuts at: 2257 2615 2257
Size: 358 3628
BstAPI GCAn_nnn'nTGC
Cuts at: 1363 1363
Size: 3986
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstYI r'GATC_y
Cuts at: 1 111 1011 1478 1501 1836 2082 3094 3180
Size: 110 900 467 23 335 246 1012 86
Cuts at: 3180 3192 3538 1
Size: 12 346 449
Fragments arranged by size:
1012 900 467 449 346 335 246 110
86 23 12
BtgI C'CryG_G
Cuts at: 107 788 107
Size: 681 3305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 628 1229 628
Size: 601 3385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 845 880 845
Size: 35 3951
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 1692 2550 1692
Size: 858 3128
EaeI y'GGCC_r
Cuts at: 1571 1745 2136 2163 3508 1571
Size: 174 391 27 1345 2049
Fragments arranged by size:
2049 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1571 1571
Size: 3986
EarI CTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2194 2504 2650 3796 2194
Size: 310 146 1146 2384
Fragments arranged by size:
2384 1146 310 146
EcoICRI GAG'CTC
Cuts at: 154 998 154
Size: 844 3142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 962 970 1811 2243 2990 3258 838
Size: 124 8 841 432 747 268 1566
Fragments arranged by size:
1566 841 747 432 268 124 8
EcoO109I rG'GnC_Cy
Cuts at: 555 1125 555
Size: 570 3416
EcoRI G'AATT_C
Cuts at: 602 990 602
Size: 388 3598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 930 2005 930
Size: 1075 2911
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 1420 1668 2690 3669 1420
Size: 248 1022 979 1737
Fragments arranged by size:
1737 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 357 389 1828 1858 1860 1890 187
Size: 32 138 32 1439 30 2 30 2283
Fragments arranged by size:
2283 1439 138 32 32 30 30 2
HincII GTy'rAC
Cuts at: 483 1025 483
Size: 542 3444
HindIII A'AGCT_T
Cuts at: 302 302
Size: 3986
KasI G'GCGC_C
Cuts at: 1416 1664 1416
Size: 248 3738
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2657 2841 2657
Size: 184 3802
MscI TGG'CCA
Cuts at: 1747 1747
Size: 3986
MslI CAynn'nnrTG
Cuts at: 281 1975 2102 281
Size: 1694 127 2165
Fragments arranged by size:
2165 1694 127
MspA1I CmG'CkG
Cuts at: 1097 1411 1771 2784 3029 1097
Size: 314 360 1013 245 2054
Fragments arranged by size:
2054 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 3986
NarI GG'CG_CC
Cuts at: 1417 1665 1417
Size: 248 3738
NcoI C'CATG_G
Cuts at: 107 788 107
Size: 681 3305
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 3986
NspI r_CATG'y
Cuts at: 1039 1539 2446 1039
Size: 500 907 2579
Fragments arranged by size:
2579 907 500
PciI A'CATG_T
Cuts at: 2442 2442
Size: 3986
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PstI C_TGCA'G
Cuts at: 1033 1718 1033
Size: 685 3301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 1411 1771 1411
Size: 360 3626
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 156 1000 156
Size: 844 3142
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
SbfI CC_TGCA'GG
Cuts at: 1033 1033
Size: 3986
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SfcI C'TryA_G
Cuts at: 33 167 779 1029 1714 2707 2898 33
Size: 134 612 250 685 993 191 1121
Fragments arranged by size:
1121 993 685 612 250 191 134
SfoI GGC'GCC
Cuts at: 1418 1666 1418
Size: 248 3738
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 445 1141 2056 2548 2810 445
Size: 696 915 492 262 1621
Fragments arranged by size:
1621 915 696 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 1039 1539 1039
Size: 500 3486
StyI C'CwwG_G
Cuts at: 107 788 1120 1474 107
Size: 681 332 354 2619
Fragments arranged by size:
2619 681 354 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1569 1702 2161 2370 1569
Size: 133 459 209 3185
Fragments arranged by size:
3185 459 209 133
TatI w'GTAC_w
Cuts at: 533 3501 533
Size: 2968 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 3954
Tth111I GACn'n_nGTC
Cuts at: 1783 1783
Size: 3986
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1481 1481
Size: 3986
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
XmnI GAAnn'nnTTC
Cuts at: 752 752
Size: 3986
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI