Restriction map for
pF1KB7601
(Circular) (Six-base) MAPSORT of: pF1KB7601.seq Check: 4052 from: 1 to: 3989
pF1KB7601 3989 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 630 644 667 745 630
Size: 14 23 78 3874
Fragments arranged by size:
3874 78 23 14
AccI GT'mk_AC
Cuts at: 386 1027 386
Size: 641 3348
Acc65I G'GTAC_C
Cuts at: 1005 1005
Size: 3989
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2246 2993 3261 1814
Size: 432 747 268 2542
Fragments arranged by size:
2542 747 432 268
AflIII A'CryG_T
Cuts at: 1341 2445 3422 1341
Size: 1104 977 1908
Fragments arranged by size:
1908 1104 977
AgeI A'CCGG_T
Cuts at: 902 2377 902
Size: 1475 2514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 3989
AlwNI CAG_nnn'CTG
Cuts at: 770 1294 2861 770
Size: 524 1567 1898
Fragments arranged by size:
1898 1567 524
ApaI G_GGCC'C
Cuts at: 496 870 982 496
Size: 374 112 3503
Fragments arranged by size:
3503 374 112
ApaLI G'TGCA_C
Cuts at: 2759 2759
Size: 3989
ApoI r'AATT_y
Cuts at: 993 3342 3598 993
Size: 2349 256 1384
Fragments arranged by size:
2349 1384 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 236 392 545 552 975 1009 236
Size: 156 153 7 423 34 3216
Fragments arranged by size:
3216 423 156 153 34 7
BamHI G'GATC_C
Cuts at: 1014 1014
Size: 3989
BanI G'GyrC_C
Cuts at: 193 222 614 694 905 1005 1419 1667 1702
Size: 29 392 80 211 100 414 248 35
Cuts at: 1702 193
Size: 2480
Fragments arranged by size:
2480 414 392 248 211 100 80 35
29
BanII G_rGCy'C
Cuts at: 496 513 552 870 982 1003 2033 496
Size: 17 39 318 112 21 1030 2452
Fragments arranged by size:
2452 1030 318 112 39 21 17
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BbsI GAAGACnn'nnnn_
Cuts at: 645 645
Size: 3989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3955
BciVI GTATCCnnnnn_n'
Cuts at: 1887 2648 3989 1887
Size: 761 1341 1887
Fragments arranged by size:
1887 1341 761
BclI T'GATC_A
Cuts at: 1509 1509
Size: 3989
BglI GCCn_nnn'nGGC
Cuts at: 403 607 403
Size: 204 3785
BglII A'GATC_T
Cuts at: 743 1504 3541 743
Size: 761 2037 1191
Fragments arranged by size:
2037 1191 761
BlpI GC'TnA_GC
Cuts at: 533 1101 533
Size: 568 3421
Bme1580I G_kGCm'C
Cuts at: 227 263 496 699 870 982 1614 1707 2763
Size: 36 233 203 171 112 632 93 1056
Cuts at: 2763 227
Size: 1453
Fragments arranged by size:
1453 1056 632 233 203 171 112 93
36
BmrI ACTGGGnnnn_n'
Cuts at: 1201 1282 1617 3761 1201
Size: 81 335 2144 1429
Fragments arranged by size:
2144 1429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 3989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 190 747 123
Size: 67 557 3365
Fragments arranged by size:
3365 557 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1165 2044 2536 2834 685
Size: 480 879 492 298 1840
Fragments arranged by size:
1840 879 492 480 298
BsaI GGTCTCn'nnnn_
Cuts at: 3693 3693
Size: 3989
BsaAI yAC'GTr
Cuts at: 1342 1342
Size: 3989
BsaBI GATnn'nnATC
Cuts at: 6 709 1503 3540 6
Size: 703 794 2037 455
Fragments arranged by size:
2037 794 703 455
BsaHI Gr'CG_yC
Cuts at: 365 890 1420 1668 2370 3866 365
Size: 525 530 248 702 1496 488
Fragments arranged by size:
1496 702 530 525 488 248
BsaWI w'CCGG_w
Cuts at: 513 902 1178 1699 2377 2651 2798 3209 513
Size: 389 276 521 678 274 147 411 1293
Fragments arranged by size:
1293 678 521 411 389 276 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 282 973 220
Size: 62 691 3236
Fragments arranged by size:
3236 691 62
BseYI C'CCAG_C
Cuts at: 275 1414 2172 2749 3245 275
Size: 1139 758 577 496 1019
Fragments arranged by size:
1139 1019 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 717 900 610
Size: 107 183 3699
Fragments arranged by size:
3699 183 107
BsiEI CG_ry'CG
Cuts at: 104 299 852 1531 1577 2785 104
Size: 195 553 679 46 1208 1308
Fragments arranged by size:
1308 1208 679 553 195 46
BsiHKAI G_wGCw'C
Cuts at: 1003 1781 1971 2763 1003
Size: 778 190 792 2229
Fragments arranged by size:
2229 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 166 166
Size: 3989
Bsp1286I G_dGCh'C
Cuts at: 227 263 496 513 552 699 870 982 1003
Size: 36 233 17 39 147 171 112 21
Cuts at: 1003 1614 1707 1781 1971 2033 2763 227
Size: 611 93 74 190 62 730 1453
Fragments arranged by size:
1453 730 611 233 190 171 147 112
93 74 62 39 36 21 17
BspEI T'CCGG_A
Cuts at: 513 3209 513
Size: 2696 1293
BspHI T'CATG_A
Cuts at: 3165 3165
Size: 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 630 644 667 745 1039 1253 1555 1936
Size: 175 14 23 78 294 214 302 381
Cuts at: 1936 455
Size: 2508
Fragments arranged by size:
2508 381 302 294 214 175 78 23
14
BsrBI CCG'CTC
Cuts at: 50 290 537 854 2281 2335 3819 3984 50
Size: 240 247 317 1427 54 1484 165 55
Fragments arranged by size:
1484 1427 317 247 240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1901 1901
Size: 3989
BsrFI r'CCGG_y
Cuts at: 161 500 617 902 908 1987 2168 2377 161
Size: 339 117 285 6 1079 181 209 1773
Fragments arranged by size:
1773 1079 339 285 209 181 117 6
BssSI C'ACGA_G
Cuts at: 2260 2618 2260
Size: 358 3631
BstAPI GCAn_nnn'nTGC
Cuts at: 668 1366 668
Size: 698 3291
BstBI TT'CG_AA
Cuts at: 2350 2350
Size: 3989
BstEII G'GTnAC_C
Cuts at: 265 265
Size: 3989
BstYI r'GATC_y
Cuts at: 1 743 1014 1481 1504 1839 2085 3097 3183
Size: 742 271 467 23 335 246 1012 86
Cuts at: 3183 3195 3541 1
Size: 12 346 449
Fragments arranged by size:
1012 742 467 449 346 335 271 246
86 23 12
BtgI C'CryG_G
Cuts at: 328 328
Size: 3989
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1939 2120 2283 1939
Size: 181 163 3645
Fragments arranged by size:
3645 181 163
BtsI GCAGTG_nn'
Cuts at: 454 573 627 828 1232 454
Size: 119 54 201 404 3211
Fragments arranged by size:
3211 404 201 119 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 729 764 729
Size: 35 3954
DraI TTT'AAA
Cuts at: 988 988
Size: 3989
DraIII CAC_nnn'GTG
Cuts at: 476 476
Size: 3989
DrdI GACnn_nn'nnGTC
Cuts at: 1695 2553 1695
Size: 858 3131
EaeI y'GGCC_r
Cuts at: 296 599 608 1574 1748 2139 2166 3511 296
Size: 303 9 966 174 391 27 1345 774
Fragments arranged by size:
1345 966 774 391 303 174 27 9
EagI C'GGCC_G
Cuts at: 296 1574 296
Size: 1278 2711
EarI CTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2197 2507 2653 3799 2197
Size: 310 146 1146 2387
Fragments arranged by size:
2387 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1001 1001
Size: 3989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 190 747 1814 2246 2993 3261 123
Size: 67 557 1067 432 747 268 851
Fragments arranged by size:
1067 851 747 557 432 268 67
EcoNI CCTnn'n_nnAGG
Cuts at: 762 762
Size: 3989
EcoO109I rG'GnC_Cy
Cuts at: 150 249 350 767 839 979 1128 150
Size: 99 101 417 72 140 149 3011
Fragments arranged by size:
3011 417 149 140 101 99 72
EcoRI G'AATT_C
Cuts at: 993 993
Size: 3989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3989
FspI TGC'GCA
Cuts at: 1770 1770
Size: 3989
HaeII r_GCGC'y
Cuts at: 915 1423 1671 2693 3672 915
Size: 508 248 1022 979 1232
Fragments arranged by size:
1232 1022 979 508 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 1831 1861 1863 1893 277
Size: 32 1522 30 2 30 2373
Fragments arranged by size:
2373 1522 32 30 30 2
HincII GTy'rAC
Cuts at: 387 1028 387
Size: 641 3348
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 1009 1009
Size: 3989
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 881 2660 2844 881
Size: 1779 184 2026
Fragments arranged by size:
2026 1779 184
MscI TGG'CCA
Cuts at: 610 1750 610
Size: 1140 2849
MslI CAynn'nnrTG
Cuts at: 1978 2105 1978
Size: 127 3862
MspA1I CmG'CkG
Cuts at: 624 1100 1414 1774 2787 3032 624
Size: 476 314 360 1013 245 1581
Fragments arranged by size:
1581 1013 476 360 314 245
NaeI GCC'GGC
Cuts at: 910 2170 910
Size: 1260 2729
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NgoMIV G'CCGG_C
Cuts at: 908 2168 908
Size: 1260 2729
NspI r_CATG'y
Cuts at: 939 1042 1542 2449 939
Size: 103 500 907 2479
Fragments arranged by size:
2479 907 500 103
PasI CC'CwG_GG
Cuts at: 281 281
Size: 3989
PciI A'CATG_T
Cuts at: 2445 2445
Size: 3989
PflMI CCAn_nnn'nTGG
Cuts at: 476 597 770 476
Size: 121 173 3695
Fragments arranged by size:
3695 173 121
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3957
PpuMI rG'GwC_Cy
Cuts at: 150 150
Size: 3989
PspOMI G'GGCC_C
Cuts at: 492 866 978 492
Size: 374 112 3503
Fragments arranged by size:
3503 374 112
PstI C_TGCA'G
Cuts at: 641 782 835 1036 1721 641
Size: 141 53 201 685 2909
Fragments arranged by size:
2909 685 201 141 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 624 1414 1774 624
Size: 790 360 2839
Fragments arranged by size:
2839 790 360
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 1003 1003
Size: 3989
SalI G'TCGA_C
Cuts at: 385 1026 385
Size: 641 3348
SanDI GG'GwC_CC
Cuts at: 150 150
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
SbfI CC_TGCA'GG
Cuts at: 641 1036 641
Size: 395 3594
ScaI AGT'ACT
Cuts at: 667 3506 667
Size: 2839 1150
SfcI C'TryA_G
Cuts at: 33 637 778 831 1032 1717 2710 2901 33
Size: 604 141 53 201 685 993 191 1121
Fragments arranged by size:
1121 993 685 604 201 191 141 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 403 607 403
Size: 204 3785
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 554 977 1011 554
Size: 423 34 3532
Fragments arranged by size:
3532 423 34
SmlI C'TyrA_G
Cuts at: 700 1144 2059 2551 2813 700
Size: 444 915 492 262 1876
Fragments arranged by size:
1876 915 492 444 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 1042 1542 1042
Size: 500 3489
SrfI GCCC'GGGC
Cuts at: 977 977
Size: 3989
StuI AGG'CCT
Cuts at: 757 757
Size: 3989
StyI C'CwwG_G
Cuts at: 368 488 1123 1477 368
Size: 120 635 354 2880
Fragments arranged by size:
2880 635 354 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 682 811 1572 1705 2164 2373 682
Size: 129 761 133 459 209 2298
Fragments arranged by size:
2298 761 459 209 133 129
TatI w'GTAC_w
Cuts at: 665 680 3504 665
Size: 15 2824 1150
Fragments arranged by size:
2824 1150 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 3957
Tth111I GACn'n_nGTC
Cuts at: 1786 1786
Size: 3989
XbaI T'CTAG_A
Cuts at: 1020 1020
Size: 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1484 1484
Size: 3989
XmaI C'CCGG_G
Cuts at: 552 975 1009 552
Size: 423 34 3532
Fragments arranged by size:
3532 423 34
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI