Restriction map for
pF1KB7602
(Circular) (Six-base) MAPSORT of: pF1KB7602.seq Check: 5489 from: 1 to: 4175
pF1KB7602 4175 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 1213 1213
Size: 4175
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 2432 3179 3447 2000
Size: 432 747 268 2728
Fragments arranged by size:
2728 747 432 268
AfeI AGC'GCT
Cuts at: 730 730
Size: 4175
AflIII A'CryG_T
Cuts at: 244 1527 2631 3608 244
Size: 1283 1104 977 811
Fragments arranged by size:
1283 1104 977 811
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 559 568 1480 3047 559
Size: 9 912 1567 1687
Fragments arranged by size:
1687 1567 912 9
ApaI G_GGCC'C
Cuts at: 167 167
Size: 4175
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 265 462 1195 265
Size: 197 733 3245
Fragments arranged by size:
3245 733 197
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 684 745 909 1075 1191 1605 1853 1888 684
Size: 61 164 166 116 414 248 35 2971
Fragments arranged by size:
2971 414 248 166 164 116 61 35
BanII G_rGCy'C
Cuts at: 167 463 469 643 1189 2219 167
Size: 296 6 174 546 1030 2123
Fragments arranged by size:
2123 1030 546 296 174 6
BbeI G_GCGC'C
Cuts at: 749 1079 1609 1857 749
Size: 330 530 248 3067
Fragments arranged by size:
3067 530 330 248
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BglI GCCn_nnn'nGGC
Cuts at: 791 791
Size: 4175
BglII A'GATC_T
Cuts at: 1690 3727 1690
Size: 2037 2138
BlpI GC'TnA_GC
Cuts at: 663 898 1287 663
Size: 235 389 3551
Fragments arranged by size:
3551 389 235
Bme1580I G_kGCm'C
Cuts at: 167 1800 1893 2949 167
Size: 1633 93 1056 1393
Fragments arranged by size:
1633 1393 1056 93
BmgBI CAC'GTC
Cuts at: 261 261
Size: 4175
BmrI ACTGGGnnnn_n'
Cuts at: 1387 1468 1803 3947 1387
Size: 81 335 2144 1615
Fragments arranged by size:
2144 1615 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 657 431
Size: 226 3949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1351 2230 2722 3020 310
Size: 1041 879 492 298 1465
Fragments arranged by size:
1465 1041 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3879 3879
Size: 4175
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 532 746 1076 1606 1854 2556 4052 532
Size: 214 330 530 248 702 1496 655
Fragments arranged by size:
1496 702 655 530 330 248 214
BsaWI w'CCGG_w
Cuts at: 1364 1885 2563 2837 2984 3395 1364
Size: 521 678 274 147 411 2144
Fragments arranged by size:
2144 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1099 1129 1099
Size: 30 4145
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 813 906 328
Size: 485 93 3597
Fragments arranged by size:
3597 485 93
BseYI C'CCAG_C
Cuts at: 150 426 552 1600 2358 2935 3431 150
Size: 276 126 1048 758 577 496 894
Fragments arranged by size:
1048 894 758 577 496 276 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 166
Size: 4175
BsiEI CG_ry'CG
Cuts at: 104 599 1717 1763 2971 104
Size: 495 1118 46 1208 1308
Fragments arranged by size:
1308 1208 1118 495 46
BsiHKAI G_wGCw'C
Cuts at: 266 463 1189 1967 2157 2949 266
Size: 197 726 778 190 792 1492
Fragments arranged by size:
1492 792 778 726 197 190
Bsp1286I G_dGCh'C
Cuts at: 167 266 463 469 643 1189 1800 1893 1967
Size: 99 197 6 174 546 611 93 74
Cuts at: 1967 2157 2219 2949 167
Size: 190 62 730 1393
Fragments arranged by size:
1393 730 611 546 197 190 174 99
93 74 62 6
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 344 1225 1439 1741 2122 344
Size: 881 214 302 381 2397
Fragments arranged by size:
2397 881 381 302 214
BsrBI CCG'CTC
Cuts at: 50 404 410 2467 2521 4005 4170 50
Size: 354 6 2057 54 1484 165 55
Fragments arranged by size:
2057 1484 354 165 55 54 6
BsrDI GCAATG_nn'
Cuts at: 2087 2087
Size: 4175
BsrFI r'CCGG_y
Cuts at: 2173 2354 2563 2173
Size: 181 209 3785
Fragments arranged by size:
3785 209 181
BssSI C'ACGA_G
Cuts at: 717 2446 2804 717
Size: 1729 358 2088
Fragments arranged by size:
2088 1729 358
BstAPI GCAn_nnn'nTGC
Cuts at: 568 1552 568
Size: 984 3191
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstYI r'GATC_y
Cuts at: 1 1200 1667 1690 2025 2271 3283 3369 3381
Size: 1199 467 23 335 246 1012 86 12
Cuts at: 3381 3727 1
Size: 346 449
Fragments arranged by size:
1199 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 975 975
Size: 4175
BtgI C'CryG_G
Cuts at: 170 191 211 905 920 170
Size: 21 20 694 15 3425
Fragments arranged by size:
3425 694 21 20 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 483 1418 483
Size: 935 3240
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DraIII CAC_nnn'GTG
Cuts at: 719 719
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 158 203 480 596 698 783 801 1760 1934
Size: 45 277 116 102 85 18 959 174
Cuts at: 1934 2325 2352 3697 158
Size: 391 27 1345 636
Fragments arranged by size:
1345 959 636 391 277 174 116 102
85 45 27 18
EagI C'GGCC_G
Cuts at: 596 1760 596
Size: 1164 3011
EarI CTCTTCn'nnn_
Cuts at: 280 546 831 2198 2408 280
Size: 266 285 1367 210 2047
Fragments arranged by size:
2047 1367 285 266 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 534 1083 2383 2693 2839 3985 534
Size: 549 1300 310 146 1146 724
Fragments arranged by size:
1300 1146 724 549 310 146
EcoICRI GAG'CTC
Cuts at: 461 1187 461
Size: 726 3449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 657 2000 2432 3179 3447 431
Size: 226 1343 432 747 268 1159
Fragments arranged by size:
1343 1159 747 432 268 226
EcoO109I rG'GnC_Cy
Cuts at: 1314 1314
Size: 4175
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 953 1956 953
Size: 1003 3172
FspAI rTGC'GCAy
Cuts at: 953 953
Size: 4175
HaeII r_GCGC'y
Cuts at: 274 325 670 732 749 868 1079 1609 1857
Size: 51 345 62 17 119 211 530 248
Cuts at: 1857 2879 3858 274
Size: 1022 979 591
Fragments arranged by size:
1022 979 591 530 345 248 211 119
62 51 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 341 373 838 870 1132 1164 2017 2047 2049
Size: 32 465 32 262 32 853 30 2
Cuts at: 2049 2079 341
Size: 30 2437
Fragments arranged by size:
2437 853 465 262 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 745 1075 1605 1853 745
Size: 330 530 248 3067
Fragments arranged by size:
3067 530 330 248
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 244 3608 244
Size: 3364 811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3030 2846
Size: 184 3991
MscI TGG'CCA
Cuts at: 482 1936 482
Size: 1454 2721
MslI CAynn'nnrTG
Cuts at: 304 947 2164 2291 304
Size: 643 1217 127 2188
Fragments arranged by size:
2188 1217 643 127
MspA1I CmG'CkG
Cuts at: 213 222 255 499 556 565 622 1081 1286
Size: 9 33 244 57 9 57 459 205
Cuts at: 1286 1600 1960 2973 3218 213
Size: 314 360 1013 245 1170
Fragments arranged by size:
1170 1013 459 360 314 245 244 205
57 57 33 9 9
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 746 1076 1606 1854 746
Size: 330 530 248 3067
Fragments arranged by size:
3067 530 330 248
NcoI C'CATG_G
Cuts at: 170 905 920 170
Size: 735 15 3425
Fragments arranged by size:
3425 735 15
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NotI GC'GGCC_GC
Cuts at: 596 596
Size: 4175
NspI r_CATG'y
Cuts at: 952 1228 1728 2635 952
Size: 276 500 907 2492
Fragments arranged by size:
2492 907 500 276
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PflMI CCAn_nnn'nTGG
Cuts at: 1167 1167
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PspOMI G'GGCC_C
Cuts at: 163 163
Size: 4175
PspXI vC'TCGA_Gb
Cuts at: 265 265
Size: 4175
PstI C_TGCA'G
Cuts at: 564 744 853 1222 1907 564
Size: 180 109 369 685 2832
Fragments arranged by size:
2832 685 369 180 109
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 255 499 622 1600 1960 255
Size: 244 123 978 360 2470
Fragments arranged by size:
2470 978 360 244 123
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 463 1189 463
Size: 726 3449
SacII CC_GC'GG
Cuts at: 214 214
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 280 546 2198 2408 280
Size: 266 1652 210 2047
Fragments arranged by size:
2047 1652 266 210
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 560 740 849 1218 1903 2896 3087 33
Size: 527 180 109 369 685 993 191 1121
Fragments arranged by size:
1121 993 685 527 369 191 180 109
SfoI GGC'GCC
Cuts at: 747 1077 1607 1855 747
Size: 330 530 248 3067
Fragments arranged by size:
3067 530 330 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 265 325 1330 2245 2737 2999 265
Size: 60 1005 915 492 262 1441
Fragments arranged by size:
1441 1005 915 492 262 60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StyI C'CwwG_G
Cuts at: 170 804 905 920 1011 1309 1663 170
Size: 634 101 15 91 298 354 2682
Fragments arranged by size:
2682 634 354 298 101 91 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 200 959 1758 1891 2350 2559 200
Size: 759 799 133 459 209 1816
Fragments arranged by size:
1816 799 759 459 209 133
TatI w'GTAC_w
Cuts at: 613 3690 613
Size: 3077 1098
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4143
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 346 1206 346
Size: 860 3315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1018 1670 1018
Size: 652 3523
XhoI C'TCGA_G
Cuts at: 265 265
Size: 4175
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PflMI PmeI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoNI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI