Restriction map for
pF1KB7613
(Circular) (Six-base) MAPSORT of: pF1KB7613.seq Check: 872 from: 1 to: 4022
pF1KB7613 4022 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 338 338
Size: 4022
AccI GT'mk_AC
Cuts at: 395 1060 395
Size: 665 3357
Acc65I G'GTAC_C
Cuts at: 644 888 1038 644
Size: 244 150 3628
Fragments arranged by size:
3628 244 150
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 782 1847 2279 3026 3294 550
Size: 232 1065 432 747 268 1278
Fragments arranged by size:
1278 1065 747 432 268 232
AflIII A'CryG_T
Cuts at: 695 1374 2478 3455 695
Size: 679 1104 977 1262
Fragments arranged by size:
1262 1104 977 679
AgeI A'CCGG_T
Cuts at: 2410 2410
Size: 4022
AlwNI CAG_nnn'CTG
Cuts at: 136 921 1327 2894 136
Size: 785 406 1567 1264
Fragments arranged by size:
1567 1264 785 406
ApaI G_GGCC'C
Cuts at: 869 869
Size: 4022
ApaLI G'TGCA_C
Cuts at: 2792 2792
Size: 4022
ApoI r'AATT_y
Cuts at: 1026 3375 3631 1026
Size: 2349 256 1417
Fragments arranged by size:
2349 1417 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 824 1042 824
Size: 218 3804
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 628 661 628
Size: 33 3989
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 151 426 644 888 1038 1452 1700 1735 151
Size: 275 218 244 150 414 248 35 2438
Fragments arranged by size:
2438 414 275 248 244 218 150 35
BanII G_rGCy'C
Cuts at: 654 869 975 1036 2066 654
Size: 215 106 61 1030 2610
Fragments arranged by size:
2610 1030 215 106 61
BbeI G_GCGC'C
Cuts at: 1456 1704 1456
Size: 248 3774
BciVI GTATCCnnnnn_n'
Cuts at: 843 1920 2681 4022 843
Size: 1077 761 1341 843
Fragments arranged by size:
1341 1077 843 761
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglII A'GATC_T
Cuts at: 525 1537 3574 525
Size: 1012 2037 973
Fragments arranged by size:
2037 1012 973
BlpI GC'TnA_GC
Cuts at: 1134 1134
Size: 4022
Bme1580I G_kGCm'C
Cuts at: 869 1647 1740 2796 869
Size: 778 93 1056 2095
Fragments arranged by size:
2095 1056 778 93
BmrI ACTGGGnnnn_n'
Cuts at: 868 1234 1315 1650 3794 868
Size: 366 81 335 2144 1096
Fragments arranged by size:
2144 1096 366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 791 316
Size: 475 3547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1198 2077 2569 2867 193
Size: 1005 879 492 298 1348
Fragments arranged by size:
1348 1005 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3726 3726
Size: 4022
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 335 1453 1701 2403 3899 335
Size: 1118 248 702 1496 458
Fragments arranged by size:
1496 1118 702 458 248
BsaWI w'CCGG_w
Cuts at: 1011 1211 1732 2410 2684 2831 3242 1011
Size: 200 521 678 274 147 411 1791
Fragments arranged by size:
1791 678 521 411 274 200 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 248 251 260 916 248
Size: 3 9 656 3354
Fragments arranged by size:
3354 656 9 3
BseYI C'CCAG_C
Cuts at: 1447 2205 2782 3278 1447
Size: 758 577 496 2191
Fragments arranged by size:
2191 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 898
Size: 4022
BsiEI CG_ry'CG
Cuts at: 104 1564 1610 2818 104
Size: 1460 46 1208 1308
Fragments arranged by size:
1460 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 654 915 975 1036 1814 2004 2796 654
Size: 261 60 61 778 190 792 1880
Fragments arranged by size:
1880 792 778 261 190 61 60
Bsp1286I G_dGCh'C
Cuts at: 654 869 915 975 1036 1647 1740 1814 2004
Size: 215 46 60 61 611 93 74 190
Cuts at: 2004 2066 2796 654
Size: 62 730 1880
Fragments arranged by size:
1880 730 611 215 190 93 74 62
61 60 46
BspEI T'CCGG_A
Cuts at: 1011 3242 1011
Size: 2231 1791
BspHI T'CATG_A
Cuts at: 3198 3198
Size: 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1072 1286 1588 1969 1072
Size: 214 302 381 3125
Fragments arranged by size:
3125 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2314 2368 3852 4017 50
Size: 2264 54 1484 165 55
Fragments arranged by size:
2264 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1934 1934
Size: 4022
BsrFI r'CCGG_y
Cuts at: 2020 2201 2410 2020
Size: 181 209 3632
Fragments arranged by size:
3632 209 181
BsrGI T'GTAC_A
Cuts at: 167 167
Size: 4022
BssSI C'ACGA_G
Cuts at: 2293 2651 2293
Size: 358 3664
BstAPI GCAn_nnn'nTGC
Cuts at: 1399 1399
Size: 4022
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstEII G'GTnAC_C
Cuts at: 372 372
Size: 4022
BstYI r'GATC_y
Cuts at: 1 462 525 1047 1514 1537 1872 2118 3130
Size: 461 63 522 467 23 335 246 1012
Cuts at: 3130 3216 3228 3574 1
Size: 86 12 346 449
Fragments arranged by size:
1012 522 467 461 449 346 335 246
86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 406 406
Size: 4022
BtgI C'CryG_G
Cuts at: 107 626 843 107
Size: 519 217 3286
Fragments arranged by size:
3286 519 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1972 2153 2316 1972
Size: 181 163 3678
Fragments arranged by size:
3678 181 163
BtsI GCAGTG_nn'
Cuts at: 1265 1265
Size: 4022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 422 457 671 706 422
Size: 35 214 35 3738
Fragments arranged by size:
3738 214 35 35
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DraIII CAC_nnn'GTG
Cuts at: 201 201
Size: 4022
DrdI GACnn_nn'nnGTC
Cuts at: 1728 2586 1728
Size: 858 3164
EaeI y'GGCC_r
Cuts at: 192 590 1607 1781 2172 2199 3544 192
Size: 398 1017 174 391 27 1345 670
Fragments arranged by size:
1345 1017 670 398 391 174 27
EagI C'GGCC_G
Cuts at: 1607 1607
Size: 4022
EarI CTCTTCn'nnn_
Cuts at: 751 2045 2255 751
Size: 1294 210 2518
Fragments arranged by size:
2518 1294 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2230 2540 2686 3832 2230
Size: 310 146 1146 2420
Fragments arranged by size:
2420 1146 310 146
EcoICRI GAG'CTC
Cuts at: 652 973 1034 652
Size: 321 61 3640
Fragments arranged by size:
3640 321 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 550 782 791 1847 2279 3026 3294 316
Size: 234 232 9 1056 432 747 268 1044
Fragments arranged by size:
1056 1044 747 432 268 234 232 9
EcoO109I rG'GnC_Cy
Cuts at: 402 865 866 1161 402
Size: 463 1 295 3263
Fragments arranged by size:
3263 463 295 1
EcoRI G'AATT_C
Cuts at: 1026 1026
Size: 4022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 762 2041 762
Size: 1279 2743
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 1456 1704 2726 3705 1456
Size: 248 1022 979 1773
Fragments arranged by size:
1773 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 447 479 717 749 1864 1894 1896 1926 447
Size: 32 238 32 1115 30 2 30 2543
Fragments arranged by size:
2543 1115 238 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
KasI G'GCGC_C
Cuts at: 1452 1700 1452
Size: 248 3774
KpnI G_GTAC'C
Cuts at: 648 892 1042 648
Size: 244 150 3628
Fragments arranged by size:
3628 244 150
MfeI C'AATT_G
Cuts at: 507 507
Size: 4022
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2877 2693
Size: 184 3838
MscI TGG'CCA
Cuts at: 194 592 1783 194
Size: 398 1191 2433
Fragments arranged by size:
2433 1191 398
MslI CAynn'nnrTG
Cuts at: 314 547 998 2011 2138 314
Size: 233 451 1013 127 2198
Fragments arranged by size:
2198 1013 451 233 127
MspA1I CmG'CkG
Cuts at: 918 1133 1447 1807 2820 3065 918
Size: 215 314 360 1013 245 1875
Fragments arranged by size:
1875 1013 360 314 245 215
NaeI GCC'GGC
Cuts at: 2203 2203
Size: 4022
NarI GG'CG_CC
Cuts at: 1453 1701 1453
Size: 248 3774
NcoI C'CATG_G
Cuts at: 107 107
Size: 4022
NdeI CA'TA_TG
Cuts at: 678 678
Size: 4022
NgoMIV G'CCGG_C
Cuts at: 2201 2201
Size: 4022
NspI r_CATG'y
Cuts at: 394 699 1075 1575 2482 394
Size: 305 376 500 907 1934
Fragments arranged by size:
1934 907 500 376 305
PciI A'CATG_T
Cuts at: 695 2478 695
Size: 1783 2239
PflMI CCAn_nnn'nTGG
Cuts at: 201 201
Size: 4022
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3990
PpuMI rG'GwC_Cy
Cuts at: 402 402
Size: 4022
PspOMI G'GGCC_C
Cuts at: 865 865
Size: 4022
PstI C_TGCA'G
Cuts at: 671 980 1069 1754 671
Size: 309 89 685 2939
Fragments arranged by size:
2939 685 309 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 918 1447 1807 918
Size: 529 360 3133
Fragments arranged by size:
3133 529 360
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 654 975 1036 654
Size: 321 61 3640
Fragments arranged by size:
3640 321 61
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SfcI C'TryA_G
Cuts at: 33 600 667 976 1065 1750 2743 2934 33
Size: 567 67 309 89 685 993 191 1121
Fragments arranged by size:
1121 993 685 567 309 191 89 67
SfoI GGC'GCC
Cuts at: 1454 1702 1454
Size: 248 3774
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 208 824 1177 2092 2584 2846 208
Size: 616 353 915 492 262 1384
Fragments arranged by size:
1384 915 616 492 353 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 1075 1575 1075
Size: 500 3522
StuI AGG'CCT
Cuts at: 251 488 251
Size: 237 3785
StyI C'CwwG_G
Cuts at: 107 1156 1510 107
Size: 1049 354 2619
Fragments arranged by size:
2619 1049 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1605 1738 2197 2406 1605
Size: 133 459 209 3221
Fragments arranged by size:
3221 459 209 133
TatI w'GTAC_w
Cuts at: 167 3537 167
Size: 3370 652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 3990
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 815 1517 815
Size: 702 3320
XhoI C'TCGA_G
Cuts at: 824 824
Size: 4022
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
XmnI GAAnn'nnTTC
Cuts at: 662 662
Size: 4022
ZraI GAC'GTC
Cuts at: 336 336
Size: 4022
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstXI BstZ17I
ClaI EcoNI EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI