Restriction map for
pF1KB7614
(Circular) (Six-base) MAPSORT of: pF1KB7614.seq Check: 7652 from: 1 to: 4193
pF1KB7614 4193 bp
With 182 enzymes: SgfI *
June 8, 2009 13:41 ..
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1069 1209 1069
Size: 140 4053
AclI AA'CG_TT
Cuts at: 1187 4033 1187
Size: 2846 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 742 2018 2450 3197 3465 586
Size: 156 1276 432 747 268 1314
Fragments arranged by size:
1314 1276 747 432 268 156
AflIII A'CryG_T
Cuts at: 695 1545 2649 3626 695
Size: 850 1104 977 1262
Fragments arranged by size:
1262 1104 977 850
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 835 867 835
Size: 32 4161
AlwNI CAG_nnn'CTG
Cuts at: 271 1498 3065 271
Size: 1227 1567 1399
Fragments arranged by size:
1567 1399 1227
ApaI G_GGCC'C
Cuts at: 341 1061 341
Size: 720 3473
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 846 1197 3546 3802 846
Size: 351 2349 256 1237
Fragments arranged by size:
2349 1237 351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 544 913 1213 544
Size: 369 300 3524
Fragments arranged by size:
3524 369 300
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 466 950 1069 1209 1623 1871 1906 466
Size: 484 119 140 414 248 35 2753
Fragments arranged by size:
2753 484 414 248 140 119 35
BanII G_rGCy'C
Cuts at: 341 449 614 914 1061 1090 1171 1207 2237
Size: 108 165 300 147 29 81 36 1030
Cuts at: 2237 341
Size: 2297
Fragments arranged by size:
2297 1030 300 165 147 108 81 36
29
BbeI G_GCGC'C
Cuts at: 954 1627 1875 954
Size: 673 248 3272
Fragments arranged by size:
3272 673 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 535 569 535
Size: 34 4159
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BfrBI ATG'CAT
Cuts at: 1179 1179
Size: 4193
BglI GCCn_nnn'nGGC
Cuts at: 177 959 177
Size: 782 3411
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 214 682 1305 214
Size: 468 623 3102
Fragments arranged by size:
3102 623 468
Bme1580I G_kGCm'C
Cuts at: 341 365 865 1061 1818 1911 2967 341
Size: 24 500 196 757 93 1056 1567
Fragments arranged by size:
1567 1056 757 500 196 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 441 2560 441
Size: 2119 2074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 254 499 514 862 134
Size: 120 245 15 348 3465
Fragments arranged by size:
3465 348 245 120 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2248 2740 3038 1369
Size: 879 492 298 2524
Fragments arranged by size:
2524 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 373 1019 3897 373
Size: 646 2878 669
Fragments arranged by size:
2878 669 646
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 1707 3744 6
Size: 1701 2037 455
Fragments arranged by size:
2037 1701 455
BsaHI Gr'CG_yC
Cuts at: 951 1624 1872 2574 4070 951
Size: 673 248 702 1496 1074
Fragments arranged by size:
1496 1074 702 673 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2855 3002 3413 1382
Size: 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 398 428 835 865 398
Size: 30 407 30 3726
Fragments arranged by size:
3726 407 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 1101 523
Size: 578 3615
BseYI C'CCAG_C
Cuts at: 191 899 1009 1618 2376 2953 3449 191
Size: 708 110 609 758 577 496 935
Fragments arranged by size:
935 758 708 609 577 496 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 808 1085 435
Size: 373 277 3543
Fragments arranged by size:
3543 373 277
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 2967 1207
Size: 778 190 792 2433
Fragments arranged by size:
2433 792 778 190
BsmI GAATG_Cn'
Cuts at: 1179 1179
Size: 4193
Bsp1286I G_dGCh'C
Cuts at: 341 365 449 614 865 914 1061 1090 1171
Size: 24 84 165 251 49 147 29 81
Cuts at: 1171 1207 1818 1911 1985 2175 2237 2967 341
Size: 36 611 93 74 190 62 730 1567
Fragments arranged by size:
1567 730 611 251 190 165 147 93
84 81 74 62 49 36 29 24
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1243 1457 1759 2140 1243
Size: 214 302 381 3296
Fragments arranged by size:
3296 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2485 2539 4023 4188 50
Size: 2435 54 1484 165 55
Fragments arranged by size:
2435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 947 2191 2372 2581 947
Size: 1244 181 209 2559
Fragments arranged by size:
2559 1244 209 181
BssSI C'ACGA_G
Cuts at: 784 2464 2822 784
Size: 1680 358 2155
Fragments arranged by size:
2155 1680 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 907 907
Size: 4193
BstYI r'GATC_y
Cuts at: 1 1218 1685 1708 2043 2289 3301 3387 3399
Size: 1217 467 23 335 246 1012 86 12
Cuts at: 3399 3745 1
Size: 346 449
Fragments arranged by size:
1217 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 392 1127 392
Size: 735 3458
BtgI C'CryG_G
Cuts at: 107 107
Size: 4193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 879 2143 2324 2487 879
Size: 1264 181 163 2585
Fragments arranged by size:
2585 1264 181 163
BtsI GCAGTG_nn'
Cuts at: 865 874 1097 1436 865
Size: 9 223 339 3622
Fragments arranged by size:
3622 339 223 9
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 1778 1952 2343 2370 3715 1778
Size: 174 391 27 1345 2256
Fragments arranged by size:
2256 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 568 2216 2426 568
Size: 1648 210 2335
Fragments arranged by size:
2335 1648 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 2711 2857 4003 2401
Size: 310 146 1146 2591
Fragments arranged by size:
2591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 254 499 514 586 742 862 2018 2450
Size: 120 245 15 72 156 120 1156 432
Cuts at: 2450 3197 3465 134
Size: 747 268 862
Fragments arranged by size:
1156 862 747 432 268 245 156 120
120 72 15
EcoNI CCTnn'n_nnAGG
Cuts at: 233 787 1022 233
Size: 554 235 3404
Fragments arranged by size:
3404 554 235
EcoO109I rG'GnC_Cy
Cuts at: 226 338 434 1057 1058 1332 226
Size: 112 96 623 1 274 3087
Fragments arranged by size:
3087 623 274 112 96 1
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 4193
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 954 1627 1875 2897 3876 954
Size: 673 248 1022 979 1271
Fragments arranged by size:
1271 1022 979 673 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 2035 2065 2067 2097 421
Size: 32 1582 30 2 30 2517
Fragments arranged by size:
2517 1582 32 30 30 2
HincII GTy'rAC
Cuts at: 1232 1232
Size: 4193
HindIII A'AGCT_T
Cuts at: 216 216
Size: 4193
KasI G'GCGC_C
Cuts at: 950 1623 1871 950
Size: 673 248 3272
Fragments arranged by size:
3272 673 248
KpnI G_GTAC'C
Cuts at: 1073 1213 1073
Size: 140 4053
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 327 2864 3048 327
Size: 2537 184 1472
Fragments arranged by size:
2537 1472 184
MscI TGG'CCA
Cuts at: 1954 1954
Size: 4193
MslI CAynn'nnrTG
Cuts at: 2182 2309 2182
Size: 127 4066
MspA1I CmG'CkG
Cuts at: 1304 1618 1978 2991 3236 1304
Size: 314 360 1013 245 2261
Fragments arranged by size:
2261 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 951 1624 1872 951
Size: 673 248 3272
Fragments arranged by size:
3272 673 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NsiI A_TGCA'T
Cuts at: 1181 1181
Size: 4193
NspI r_CATG'y
Cuts at: 699 1246 1746 2653 699
Size: 547 500 907 2239
Fragments arranged by size:
2239 907 547 500
PasI CC'CwG_GG
Cuts at: 585 618 1054 585
Size: 33 436 3724
Fragments arranged by size:
3724 436 33
PciI A'CATG_T
Cuts at: 695 2649 695
Size: 1954 2239
PflMI CCAn_nnn'nTGG
Cuts at: 157 1081 157
Size: 924 3269
PfoI T'CCnGG_A
Cuts at: 231 231
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 835 867 3332 3364 835
Size: 32 2465 32 1664
Fragments arranged by size:
2465 1664 32 32
PshAI GACnn'nnGTC
Cuts at: 1035 1035
Size: 4193
PspOMI G'GGCC_C
Cuts at: 337 1057 337
Size: 720 3473
PspXI vC'TCGA_Gb
Cuts at: 544 544
Size: 4193
PstI C_TGCA'G
Cuts at: 536 680 722 860 881 1240 1925 536
Size: 144 42 138 21 359 685 2804
Fragments arranged by size:
2804 685 359 144 138 42 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 1618 1978 1618
Size: 360 3833
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SfcI C'TryA_G
Cuts at: 33 532 676 718 856 877 1236 1921 2914
Size: 499 144 42 138 21 359 685 993
Cuts at: 2914 3105 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 499 359 191 144 138
42 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 177 177
Size: 4193
SfoI GGC'GCC
Cuts at: 952 1625 1873 952
Size: 673 248 3272
Fragments arranged by size:
3272 673 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 544 913 1348 2263 2755 3017 544
Size: 369 435 915 492 262 1720
Fragments arranged by size:
1720 915 492 435 369 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 107 222 366 1327 1681 107
Size: 115 144 961 354 2619
Fragments arranged by size:
2619 961 354 144 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 717 1104 1776 1909 2368 2577 717
Size: 387 672 133 459 209 2333
Fragments arranged by size:
2333 672 459 387 209 133
TatI w'GTAC_w
Cuts at: 1106 3708 1106
Size: 2602 1591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 398 430 3710 3742 398
Size: 32 3280 32 849
Fragments arranged by size:
3280 849 32 32
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 229 1688 229
Size: 1459 2734
XhoI C'TCGA_G
Cuts at: 544 913 544
Size: 369 3824
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
XmnI GAAnn'nnTTC
Cuts at: 640 755 850 1139 640
Size: 115 95 289 3694
Fragments arranged by size:
3694 289 115 95
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PmlI PpuMI PsiI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI ZraI