Restriction map for
pF1KB7615
(Circular) (Six-base) MAPSORT of: pF1KB7615.seq Check: 5063 from: 1 to: 4025
pF1KB7615 4025 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 582 582
Size: 4025
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1850 2282 3029 3297 1850
Size: 432 747 268 2578
Fragments arranged by size:
2578 747 432 268
AflIII A'CryG_T
Cuts at: 1377 2481 3458 1377
Size: 1104 977 1944
Fragments arranged by size:
1944 1104 977
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AhdI GACnn_n'nnGTC
Cuts at: 213 213
Size: 4025
AleI CACnn'nnGTG
Cuts at: 989 989
Size: 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 878 878
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 369 431 567 1330 2897 369
Size: 62 136 763 1567 1497
Fragments arranged by size:
1567 1497 763 136 62
ApaI G_GGCC'C
Cuts at: 458 458
Size: 4025
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 721 1029 3378 3634 721
Size: 308 2349 256 1112
Fragments arranged by size:
2349 1112 308 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 223 869 1045 223
Size: 646 176 3203
Fragments arranged by size:
3203 646 176
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 308 383 1041 1455 1703 1738 308
Size: 75 658 414 248 35 2595
Fragments arranged by size:
2595 658 414 248 75 35
BanII G_rGCy'C
Cuts at: 230 458 514 1039 2069 230
Size: 228 56 525 1030 2186
Fragments arranged by size:
2186 1030 525 228 56
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BbsI GAAGACnn'nnnn_
Cuts at: 392 983 392
Size: 591 3434
BciVI GTATCCnnnnn_n'
Cuts at: 256 1923 2684 4025 256
Size: 1667 761 1341 256
Fragments arranged by size:
1667 1341 761 256
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BglII A'GATC_T
Cuts at: 202 1540 3577 202
Size: 1338 2037 650
Fragments arranged by size:
2037 1338 650
BlpI GC'TnA_GC
Cuts at: 1137 1137
Size: 4025
Bme1580I G_kGCm'C
Cuts at: 311 458 1650 1743 2799 311
Size: 147 1192 93 1056 1537
Fragments arranged by size:
1537 1192 1056 147 93
BmrI ACTGGGnnnn_n'
Cuts at: 536 1237 1318 1653 3797 536
Size: 701 81 335 2144 764
Fragments arranged by size:
2144 764 701 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 434 2392 434
Size: 1958 2067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 470 498 931 421
Size: 49 28 433 3515
Fragments arranged by size:
3515 433 49 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 2080 2572 2870 1201
Size: 879 492 298 2356
Fragments arranged by size:
2356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1214 1735 2413 2687 2834 3245 1214
Size: 521 678 274 147 411 1994
Fragments arranged by size:
1994 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 157
Size: 30 3995
BseYI C'CCAG_C
Cuts at: 1450 2208 2785 3281 1450
Size: 758 577 496 2194
Fragments arranged by size:
2194 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 956
Size: 4025
BsiEI CG_ry'CG
Cuts at: 104 558 1567 1613 2821 104
Size: 454 1009 46 1208 1308
Fragments arranged by size:
1308 1208 1009 454 46
BsiHKAI G_wGCw'C
Cuts at: 230 493 1039 1817 2007 2799 230
Size: 263 546 778 190 792 1456
Fragments arranged by size:
1456 792 778 546 263 190
Bsp1286I G_dGCh'C
Cuts at: 230 311 458 493 514 1039 1650 1743 1817
Size: 81 147 35 21 525 611 93 74
Cuts at: 1817 2007 2069 2799 230
Size: 190 62 730 1456
Fragments arranged by size:
1456 730 611 525 190 147 93 81
74 62 35 21
BspEI T'CCGG_A
Cuts at: 3245 3245
Size: 4025
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 582 1075 1289 1591 1972 582
Size: 493 214 302 381 2635
Fragments arranged by size:
2635 493 381 302 214
BsrBI CCG'CTC
Cuts at: 50 120 2317 2371 3855 4020 50
Size: 70 2197 54 1484 165 55
Fragments arranged by size:
2197 1484 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 2023 2204 2413 2023
Size: 181 209 3635
Fragments arranged by size:
3635 209 181
BssHII G'CGCG_C
Cuts at: 539 539
Size: 4025
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 162 724 1402 162
Size: 562 678 2785
Fragments arranged by size:
2785 678 562
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstYI r'GATC_y
Cuts at: 1 202 780 1050 1517 1540 1875 2121 3133
Size: 201 578 270 467 23 335 246 1012
Cuts at: 3133 3219 3231 3577 1
Size: 86 12 346 449
Fragments arranged by size:
1012 578 467 449 346 335 270 246
201 86 23 12
BtgI C'CryG_G
Cuts at: 107 149 195 107
Size: 42 46 3937
Fragments arranged by size:
3937 46 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 776 1268 776
Size: 492 3533
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DraIII CAC_nnn'GTG
Cuts at: 412 412
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 555 1610 1784 2175 2202 3547 555
Size: 1055 174 391 27 1345 1033
Fragments arranged by size:
1345 1055 1033 391 174 27
EagI C'GGCC_G
Cuts at: 555 1610 555
Size: 1055 2970
EarI CTCTTCn'nnn_
Cuts at: 269 504 829 2048 2258 269
Size: 235 325 1219 210 2036
Fragments arranged by size:
2036 1219 325 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 598 837 2233 2543 2689 3835 598
Size: 239 1396 310 146 1146 788
Fragments arranged by size:
1396 1146 788 310 239 146
EcoICRI GAG'CTC
Cuts at: 228 1037 228
Size: 809 3216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 470 498 931 1850 2282 3029 3297 421
Size: 49 28 433 919 432 747 268 1149
Fragments arranged by size:
1149 919 747 433 432 268 49 28
EcoO109I rG'GnC_Cy
Cuts at: 454 455 1164 454
Size: 1 709 3315
Fragments arranged by size:
3315 709 1
EcoRI G'AATT_C
Cuts at: 1029 1029
Size: 4025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 4025
FspI TGC'GCA
Cuts at: 136 1013 1806 136
Size: 877 793 2355
Fragments arranged by size:
2355 877 793
FspAI rTGC'GCAy
Cuts at: 1013 1013
Size: 4025
HaeII r_GCGC'y
Cuts at: 1459 1707 2729 3708 1459
Size: 248 1022 979 1776
Fragments arranged by size:
1776 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 985 1017 1867 1897 1899 1929 199
Size: 32 754 32 850 30 2 30 2295
Fragments arranged by size:
2295 850 754 32 32 30 30 2
HincII GTy'rAC
Cuts at: 657 1064 657
Size: 407 3618
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 518 2696 2880 518
Size: 2178 184 1663
Fragments arranged by size:
2178 1663 184
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 989 2014 2141 989
Size: 1025 127 2873
Fragments arranged by size:
2873 1025 127
MspA1I CmG'CkG
Cuts at: 1136 1450 1810 2823 3068 1136
Size: 314 360 1013 245 2093
Fragments arranged by size:
2093 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NcoI C'CATG_G
Cuts at: 107 195 107
Size: 88 3937
NdeI CA'TA_TG
Cuts at: 212 212
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NspI r_CATG'y
Cuts at: 285 1012 1078 1578 2485 285
Size: 727 66 500 907 1825
Fragments arranged by size:
1825 907 727 500 66
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 104 136 3164 3196 104
Size: 32 3028 32 933
Fragments arranged by size:
3028 933 32 32
PspOMI G'GGCC_C
Cuts at: 454 454
Size: 4025
PstI C_TGCA'G
Cuts at: 1072 1757 1072
Size: 685 3340
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 230 1039 230
Size: 809 3216
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 965 1068 1753 2746 2937 33
Size: 932 103 685 993 191 1121
Fragments arranged by size:
1121 993 932 685 191 103
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 1180 2095 2587 2849 1180
Size: 915 492 262 2356
Fragments arranged by size:
2356 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1012 1078 1578 1012
Size: 66 500 3459
Fragments arranged by size:
3459 500 66
StyI C'CwwG_G
Cuts at: 107 195 1159 1513 107
Size: 88 964 354 2619
Fragments arranged by size:
2619 964 354 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1608 1741 2200 2409 1608
Size: 133 459 209 3224
Fragments arranged by size:
3224 459 209 133
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 3542 3574 157
Size: 32 3353 32 608
Fragments arranged by size:
3353 608 32 32
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 445 1520 445
Size: 1075 2950
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI