Restriction map for
pF1KB7618
(Circular) (Six-base) MAPSORT of: pF1KB7618.seq Check: 312 from: 1 to: 4199
pF1KB7618 4199 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1237 1237
Size: 4199
Acc65I G'GTAC_C
Cuts at: 713 1215 713
Size: 502 3697
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 2024 2456 3203 3471 1015
Size: 1009 432 747 268 1743
Fragments arranged by size:
1743 1009 747 432 268
AflIII A'CryG_T
Cuts at: 944 1551 2655 3632 944
Size: 607 1104 977 1511
Fragments arranged by size:
1511 1104 977 607
AgeI A'CCGG_T
Cuts at: 609 2587 609
Size: 1978 2221
AlwNI CAG_nnn'CTG
Cuts at: 676 1504 3071 676
Size: 828 1567 1804
Fragments arranged by size:
1804 1567 828
ApaI G_GGCC'C
Cuts at: 163 229 361 559 626 163
Size: 66 132 198 67 3736
Fragments arranged by size:
3736 198 132 67 66
ApaLI G'TGCA_C
Cuts at: 2969 2969
Size: 4199
ApoI r'AATT_y
Cuts at: 1203 3552 3808 1203
Size: 2349 256 1594
Fragments arranged by size:
2349 1594 256
AscI GG'CGCG_CC
Cuts at: 374 374
Size: 4199
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 228 283 354 436 465 504 533 583 618
Size: 55 71 82 29 39 29 50 35
Cuts at: 618 639 829 850 957 1219 228
Size: 21 190 21 107 262 3208
Fragments arranged by size:
3208 262 190 107 82 71 55 50
39 35 29 29 21 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 598 631 598
Size: 33 4166
BamHI G'GATC_C
Cuts at: 867 1224 867
Size: 357 3842
BanI G'GyrC_C
Cuts at: 396 628 688 713 732 1215 1629 1877 1912
Size: 232 60 25 19 483 414 248 35
Cuts at: 1912 396
Size: 2683
Fragments arranged by size:
2683 483 414 248 232 60 35 25
19
BanII G_rGCy'C
Cuts at: 163 229 361 368 534 540 559 590 626
Size: 66 132 7 166 6 19 31 36
Cuts at: 626 773 988 1213 2243 163
Size: 147 215 225 1030 2119
Fragments arranged by size:
2119 1030 225 215 166 147 132 66
36 31 19 7 6
BbeI G_GCGC'C
Cuts at: 400 632 692 736 1633 1881 400
Size: 232 60 44 897 248 2718
Fragments arranged by size:
2718 897 248 232 60 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 831 865 831
Size: 34 4165
BciVI GTATCCnnnnn_n'
Cuts at: 2097 2858 4199 2097
Size: 761 1341 2097
Fragments arranged by size:
2097 1341 761
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglI GCCn_nnn'nGGC
Cuts at: 415 415
Size: 4199
BglII A'GATC_T
Cuts at: 1714 3751 1714
Size: 2037 2162
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 163 229 361 559 626 752 844 1824 1917
Size: 66 132 198 67 126 92 980 93
Cuts at: 1917 2973 163
Size: 1056 1389
Fragments arranged by size:
1389 1056 980 198 132 126 93 92
67 66
BmgBI CAC'GTC
Cuts at: 800 800
Size: 4199
BmrI ACTGGGnnnn_n'
Cuts at: 1411 1492 1827 3971 1411
Size: 81 335 2144 1639
Fragments arranged by size:
2144 1639 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 519 2566 519
Size: 2047 2152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 757 1080 554
Size: 203 323 3673
Fragments arranged by size:
3673 323 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1375 2254 2746 3044 175
Size: 1200 879 492 298 1330
Fragments arranged by size:
1330 1200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 115 880 3903 115
Size: 765 3023 411
Fragments arranged by size:
3023 765 411
BsaAI yAC'GTr
Cuts at: 264 947 1043 1144 1552 264
Size: 683 96 101 408 2911
Fragments arranged by size:
2911 683 408 101 96
BsaBI GATnn'nnATC
Cuts at: 6 1713 3750 6
Size: 1707 2037 455
Fragments arranged by size:
2037 1707 455
BsaHI Gr'CG_yC
Cuts at: 317 397 545 629 682 689 733 1630 1878
Size: 80 148 84 53 7 44 897 248
Cuts at: 1878 2580 4076 317
Size: 702 1496 440
Fragments arranged by size:
1496 897 702 440 248 148 84 80
53 44 7
BsaWI w'CCGG_w
Cuts at: 609 1097 1388 1909 2587 2861 3008 3419 609
Size: 488 291 521 678 274 147 411 1389
Fragments arranged by size:
1389 678 521 488 411 291 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 331 627 328
Size: 3 296 3900
Fragments arranged by size:
3900 296 3
BseYI C'CCAG_C
Cuts at: 400 478 558 1624 2382 2959 3455 400
Size: 78 80 1066 758 577 496 1144
Fragments arranged by size:
1144 1066 758 577 496 80 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1023 1023
Size: 4199
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 2995 104
Size: 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 988 1213 1991 2181 2973 988
Size: 225 778 190 792 2214
Fragments arranged by size:
2214 792 778 225 190
Bsp1286I G_dGCh'C
Cuts at: 163 229 361 368 534 540 559 590 626
Size: 66 132 7 166 6 19 31 36
Cuts at: 626 752 773 844 988 1213 1824 1917 1991
Size: 126 21 71 144 225 611 93 74
Cuts at: 1991 2181 2243 2973 163
Size: 190 62 730 1389
Fragments arranged by size:
1389 730 611 225 190 166 144 132
126 93 74 71 66 62 36 31
21 19 7 6
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 3375 3375
Size: 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 394 1249 1463 1765 2146 394
Size: 855 214 302 381 2447
Fragments arranged by size:
2447 855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2491 2545 4029 4194 50
Size: 2441 54 1484 165 55
Fragments arranged by size:
2441 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2111 2111
Size: 4199
BsrFI r'CCGG_y
Cuts at: 609 631 2197 2378 2587 609
Size: 22 1566 181 209 2221
Fragments arranged by size:
2221 1566 209 181 22
BssHII G'CGCG_C
Cuts at: 374 922 950 1069 374
Size: 548 28 119 3504
Fragments arranged by size:
3504 548 119 28
BssSI C'ACGA_G
Cuts at: 2470 2828 2470
Size: 358 3841
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 1576
Size: 4199
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 597 597
Size: 4199
BstYI r'GATC_y
Cuts at: 1 333 867 1224 1691 1714 2049 2295 3307
Size: 332 534 357 467 23 335 246 1012
Cuts at: 3307 3393 3405 3751 1
Size: 86 12 346 449
Fragments arranged by size:
1012 534 467 449 357 346 335 332
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 245 655 765 107
Size: 138 410 110 3541
Fragments arranged by size:
3541 410 138 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 736 2149 2330 2493 736
Size: 1413 181 163 2442
Fragments arranged by size:
2442 1413 181 163
BtsI GCAGTG_nn'
Cuts at: 1442 1442
Size: 4199
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 1905 2763 1905
Size: 858 3341
EaeI y'GGCC_r
Cuts at: 110 1784 1958 2349 2376 3721 110
Size: 1674 174 391 27 1345 588
Fragments arranged by size:
1674 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 203 287 766 2222 2432 203
Size: 84 479 1456 210 1970
Fragments arranged by size:
1970 1456 479 210 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 416 2407 2717 2863 4009 416
Size: 1991 310 146 1146 606
Fragments arranged by size:
1991 1146 606 310 146
EcoICRI GAG'CTC
Cuts at: 986 1211 986
Size: 225 3974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 757 1015 1080 2024 2456 3203 3471 554
Size: 203 258 65 944 432 747 268 1282
Fragments arranged by size:
1282 944 747 432 268 258 203 65
EcoO109I rG'GnC_Cy
Cuts at: 160 232 327 622 778 826 1338 160
Size: 72 95 295 156 48 512 3021
Fragments arranged by size:
3021 512 295 156 95 72 48
EcoRI G'AATT_C
Cuts at: 1203 1203
Size: 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 4199
FspI TGC'GCA
Cuts at: 1009 1980 1009
Size: 971 3228
HaeII r_GCGC'y
Cuts at: 204 400 598 604 632 692 736 1119 1188
Size: 196 198 6 28 60 44 383 69
Cuts at: 1188 1633 1881 2903 3882 204
Size: 445 248 1022 979 521
Fragments arranged by size:
1022 979 521 445 383 248 198 196
69 60 44 28 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 375 407 2041 2071 2073 2103 375
Size: 32 1634 30 2 30 2471
Fragments arranged by size:
2471 1634 32 30 30 2
HincII GTy'rAC
Cuts at: 1238 1238
Size: 4199
KasI G'GCGC_C
Cuts at: 396 628 688 732 1629 1877 396
Size: 232 60 44 897 248 2718
Fragments arranged by size:
2718 897 248 232 60 44
KpnI G_GTAC'C
Cuts at: 717 1219 717
Size: 502 3697
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3054 2870
Size: 184 4015
MscI TGG'CCA
Cuts at: 112 1960 112
Size: 1848 2351
MslI CAynn'nnrTG
Cuts at: 2188 2315 2188
Size: 127 4072
MspA1I CmG'CkG
Cuts at: 657 767 1127 1310 1624 1984 2997 3242 657
Size: 110 360 183 314 360 1013 245 1614
Fragments arranged by size:
1614 1013 360 360 314 245 183 110
NaeI GCC'GGC
Cuts at: 633 2380 633
Size: 1747 2452
NarI GG'CG_CC
Cuts at: 397 629 689 733 1630 1878 397
Size: 232 60 44 897 248 2718
Fragments arranged by size:
2718 897 248 232 60 44
NcoI C'CATG_G
Cuts at: 107 107
Size: 4199
NdeI CA'TA_TG
Cuts at: 453 453
Size: 4199
NgoMIV G'CCGG_C
Cuts at: 631 2378 631
Size: 1747 2452
NspI r_CATG'y
Cuts at: 1252 1752 2659 1252
Size: 500 907 2792
Fragments arranged by size:
2792 907 500
PasI CC'CwG_GG
Cuts at: 156 156
Size: 4199
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PfoI T'CCnGG_A
Cuts at: 167 167
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PmlI CAC'GTG
Cuts at: 264 947 1043 1144 264
Size: 683 96 101 3319
Fragments arranged by size:
3319 683 101 96
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PpuMI rG'GwC_Cy
Cuts at: 232 327 778 826 232
Size: 95 451 48 3605
Fragments arranged by size:
3605 451 95 48
PspOMI G'GGCC_C
Cuts at: 159 225 357 555 622 159
Size: 66 132 198 67 3736
Fragments arranged by size:
3736 198 132 67 66
PstI C_TGCA'G
Cuts at: 864 1246 1931 864
Size: 382 685 3132
Fragments arranged by size:
3132 685 382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 1624 1984 1624
Size: 360 3839
RsrII CG'GwC_CG
Cuts at: 659 2394 659
Size: 1735 2464
SacI G_AGCT'C
Cuts at: 988 1213 988
Size: 225 3974
SacII CC_GC'GG
Cuts at: 658 768 658
Size: 110 4089
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SanDI GG'GwC_CC
Cuts at: 778 826 778
Size: 48 4151
SapI GCTCTTCn'nnn_
Cuts at: 766 2222 2432 766
Size: 1456 210 2533
Fragments arranged by size:
2533 1456 210
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 860 1242 1927 2920 3111 33
Size: 827 382 685 993 191 1121
Fragments arranged by size:
1121 993 827 685 382 191
SfoI GGC'GCC
Cuts at: 398 630 690 734 1631 1879 398
Size: 232 60 44 897 248 2718
Fragments arranged by size:
2718 897 248 232 60 44
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 230 285 467 535 620 831 959 1221 230
Size: 55 182 68 85 211 128 262 3208
Fragments arranged by size:
3208 262 211 182 128 85 68 55
SmlI C'TyrA_G
Cuts at: 190 1354 2269 2761 3023 190
Size: 1164 915 492 262 1366
Fragments arranged by size:
1366 1164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
SrfI GCCC'GGGC
Cuts at: 535 535
Size: 4199
StuI AGG'CCT
Cuts at: 409 409
Size: 4199
StyI C'CwwG_G
Cuts at: 107 590 744 1333 1687 107
Size: 483 154 589 354 2619
Fragments arranged by size:
2619 589 483 354 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 501 1782 1915 2374 2583 501
Size: 1281 133 459 209 2117
Fragments arranged by size:
2117 1281 459 209 133
TatI w'GTAC_w
Cuts at: 3714 3714
Size: 4199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4167
Tth111I GACn'n_nGTC
Cuts at: 1996 1996
Size: 4199
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 1694 327
Size: 1367 2832
XmaI C'CCGG_G
Cuts at: 228 283 465 533 618 829 957 1219 228
Size: 55 182 68 85 211 128 262 3208
Fragments arranged by size:
3208 262 211 182 128 85 68 55
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AvrII BbsI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PflMI PshAI PsiI
PspXI PsrI SexAI SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI