Restriction map for
pF1KB7619
(Circular) (Six-base) MAPSORT of: pF1KB7619.seq Check: 9746 from: 1 to: 4037
pF1KB7619 4037 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1075 1075
Size: 4037
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1862 2294 3041 3309 772
Size: 1090 432 747 268 1500
Fragments arranged by size:
1500 1090 747 432 268
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 184 216 184
Size: 32 4005
AlwNI CAG_nnn'CTG
Cuts at: 1342 2909 1342
Size: 1567 2470
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 243 376 977 1041 3390 3646 243
Size: 133 601 64 2349 256 634
Fragments arranged by size:
2349 634 601 256 133 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 1057 1057
Size: 4037
BamHI G'GATC_C
Cuts at: 511 1062 511
Size: 551 3486
BanI G'GyrC_C
Cuts at: 756 1053 1467 1715 1750 756
Size: 297 414 248 35 3043
Fragments arranged by size:
3043 414 297 248 35
BanII G_rGCy'C
Cuts at: 713 1051 2081 713
Size: 338 1030 2669
Fragments arranged by size:
2669 1030 338
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BbsI GAAGACnn'nnnn_
Cuts at: 617 617
Size: 4037
BbvCI CC'TCA_GC
Cuts at: 423 423
Size: 4037
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BfrBI ATG'CAT
Cuts at: 218 218
Size: 4037
BglII A'GATC_T
Cuts at: 456 820 1552 3589 456
Size: 364 732 2037 904
Fragments arranged by size:
2037 904 732 364
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 1662 1755 2811 1662
Size: 93 1056 2888
Fragments arranged by size:
2888 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 838 1249 1330 1665 3809 838
Size: 411 81 335 2144 1066
Fragments arranged by size:
2144 1066 411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 974
Size: 4037
Bpu10I CC'TnA_GC
Cuts at: 423 423
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2092 2584 2882 1213
Size: 879 492 298 2368
Fragments arranged by size:
2368 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 350 3741 350
Size: 3391 646
BsaAI yAC'GTr
Cuts at: 959 1390 959
Size: 431 3606
BsaBI GATnn'nnATC
Cuts at: 6 812 1551 3588 6
Size: 806 739 2037 455
Fragments arranged by size:
2037 806 739 455
BsaHI Gr'CG_yC
Cuts at: 1468 1716 2418 3914 1468
Size: 248 702 1496 1591
Fragments arranged by size:
1591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 703
Size: 4037
BseYI C'CCAG_C
Cuts at: 237 1462 2220 2797 3293 237
Size: 1225 758 577 496 981
Fragments arranged by size:
1225 981 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1051 1829 2019 2811 1051
Size: 778 190 792 2277
Fragments arranged by size:
2277 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 713 1051 1662 1755 1829 2019 2081 2811 713
Size: 338 611 93 74 190 62 730 1939
Fragments arranged by size:
1939 730 611 338 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 257 638 3213 257
Size: 381 2575 1081
Fragments arranged by size:
2575 1081 381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1301 1603 1984 1087
Size: 214 302 381 3140
Fragments arranged by size:
3140 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 2035 2216 2425 2035
Size: 181 209 3647
Fragments arranged by size:
3647 209 181
BssSI C'ACGA_G
Cuts at: 2308 2666 2308
Size: 358 3679
BstAPI GCAn_nnn'nTGC
Cuts at: 786 1414 786
Size: 628 3409
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 170 170
Size: 4037
BstYI r'GATC_y
Cuts at: 1 456 511 820 1062 1529 1552 1887 2133
Size: 455 55 309 242 467 23 335 246
Cuts at: 2133 3145 3231 3243 3589 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 455 449 346 335 309 246
242 86 55 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 1280 1280
Size: 4037
DraI TTT'AAA
Cuts at: 975 1036 975
Size: 61 3976
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 518 1622 1796 2187 2214 3559 518
Size: 1104 174 391 27 1345 996
Fragments arranged by size:
1345 1104 996 391 174 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 440 779 2060 2270 440
Size: 339 1281 210 2207
Fragments arranged by size:
2207 1281 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 974 1862 2294 3041 3309 772
Size: 202 888 432 747 268 1500
Fragments arranged by size:
1500 888 747 432 268 202
EcoNI CCTnn'n_nnAGG
Cuts at: 718 718
Size: 4037
EcoO109I rG'GnC_Cy
Cuts at: 1176 1176
Size: 4037
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 4037
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 1471 1719 2741 3720 1471
Size: 248 1022 979 1788
Fragments arranged by size:
1788 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 441 473 688 720 756 788 805
Size: 32 133 32 215 32 36 32 17
Cuts at: 805 837 1879 1909 1911 1941 276
Size: 32 1042 30 2 30 2372
Fragments arranged by size:
2372 1042 215 133 36 32 32 32
32 32 30 30 17 2
HincII GTy'rAC
Cuts at: 487 855 1076 487
Size: 368 221 3448
Fragments arranged by size:
3448 368 221
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 293 725 741 2708 2892 293
Size: 432 16 1967 184 1438
Fragments arranged by size:
1967 1438 432 184 16
MscI TGG'CCA
Cuts at: 520 1798 520
Size: 1278 2759
MslI CAynn'nnrTG
Cuts at: 2026 2153 2026
Size: 127 3910
MspA1I CmG'CkG
Cuts at: 1148 1462 1822 2835 3080 1148
Size: 314 360 1013 245 2105
Fragments arranged by size:
2105 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NcoI C'CATG_G
Cuts at: 107 107
Size: 4037
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NsiI A_TGCA'T
Cuts at: 220 220
Size: 4037
NspI r_CATG'y
Cuts at: 1090 1590 2497 1090
Size: 500 907 2630
Fragments arranged by size:
2630 907 500
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PfoI T'CCnGG_A
Cuts at: 321 321
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PstI C_TGCA'G
Cuts at: 609 782 1084 1769 609
Size: 173 302 685 2877
Fragments arranged by size:
2877 685 302 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 1462 1822 1462
Size: 360 3677
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 605 778 858 1080 1765 2758 2949 33
Size: 572 173 80 222 685 993 191 1121
Fragments arranged by size:
1121 993 685 572 222 191 173 80
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 1192 2107 2599 2861 1192
Size: 915 492 262 2368
Fragments arranged by size:
2368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
SspI AAT'ATT
Cuts at: 672 929 672
Size: 257 3780
StyI C'CwwG_G
Cuts at: 107 1171 1525 107
Size: 1064 354 2619
Fragments arranged by size:
2619 1064 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 783 1620 1753 2212 2421 783
Size: 837 133 459 209 2399
Fragments arranged by size:
2399 837 459 209 133
TatI w'GTAC_w
Cuts at: 952 3552 952
Size: 2600 1437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1532 1532
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI