Restriction map for
pF1KB7620
(Circular) (Six-base) MAPSORT of: pF1KB7620.seq Check: 4720 from: 1 to: 4214
pF1KB7620 4214 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 796 796
Size: 4214
AccI GT'mk_AC
Cuts at: 1252 1252
Size: 4214
Acc65I G'GTAC_C
Cuts at: 1230 1230
Size: 4214
AclI AA'CG_TT
Cuts at: 4054 4054
Size: 4214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 892 1013 2039 2471 3218 3486 429
Size: 463 121 1026 432 747 268 1157
Fragments arranged by size:
1157 1026 747 463 432 268 121
AflIII A'CryG_T
Cuts at: 1566 2670 3647 1566
Size: 1104 977 2133
Fragments arranged by size:
2133 1104 977
AgeI A'CCGG_T
Cuts at: 2602 2602
Size: 4214
AhdI GACnn_n'nnGTC
Cuts at: 956 956
Size: 4214
AleI CACnn'nnGTG
Cuts at: 317 1085 317
Size: 768 3446
AlwNI CAG_nnn'CTG
Cuts at: 1102 1519 3086 1102
Size: 417 1567 2230
Fragments arranged by size:
2230 1567 417
ApaI G_GGCC'C
Cuts at: 779 845 779
Size: 66 4148
ApaLI G'TGCA_C
Cuts at: 2984 2984
Size: 4214
ApoI r'AATT_y
Cuts at: 1218 3567 3823 1218
Size: 2349 256 1609
Fragments arranged by size:
2349 1609 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 547 1234 547
Size: 687 3527
BamHI G'GATC_C
Cuts at: 1239 1239
Size: 4214
BanI G'GyrC_C
Cuts at: 534 618 748 892 1230 1644 1892 1927 534
Size: 84 130 144 338 414 248 35 2821
Fragments arranged by size:
2821 414 338 248 144 130 84 35
BanII G_rGCy'C
Cuts at: 779 836 845 1228 2258 779
Size: 57 9 383 1030 2735
Fragments arranged by size:
2735 1030 383 57 9
BbeI G_GCGC'C
Cuts at: 1648 1896 1648
Size: 248 3966
BbsI GAAGACnn'nnnn_
Cuts at: 1132 1132
Size: 4214
BciVI GTATCCnnnnn_n'
Cuts at: 2112 2873 4214 2112
Size: 761 1341 2112
Fragments arranged by size:
2112 1341 761
BclI T'GATC_A
Cuts at: 1734 1734
Size: 4214
BglI GCCn_nnn'nGGC
Cuts at: 361 840 361
Size: 479 3735
BglII A'GATC_T
Cuts at: 1729 3766 1729
Size: 2037 2177
BlpI GC'TnA_GC
Cuts at: 577 1326 577
Size: 749 3465
Bme1580I G_kGCm'C
Cuts at: 779 845 1052 1839 1932 2988 779
Size: 66 207 787 93 1056 2005
Fragments arranged by size:
2005 1056 787 207 93 66
BmrI ACTGGGnnnn_n'
Cuts at: 110 799 1426 1507 1842 3986 110
Size: 689 627 81 335 2144 338
Fragments arranged by size:
2144 689 627 338 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 641 742 848 865 950 1127 352
Size: 289 101 106 17 85 177 3439
Fragments arranged by size:
3439 289 177 106 101 85 17
Bpu10I CC'TnA_GC
Cuts at: 1204 1204
Size: 4214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1390 2269 2761 3059 253
Size: 1137 879 492 298 1408
Fragments arranged by size:
1408 1137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 163 170 3918 163
Size: 7 3748 459
Fragments arranged by size:
3748 459 7
BsaAI yAC'GTr
Cuts at: 1567 1567
Size: 4214
BsaBI GATnn'nnATC
Cuts at: 6 1728 3765 6
Size: 1722 2037 455
Fragments arranged by size:
2037 1722 455
BsaHI Gr'CG_yC
Cuts at: 1645 1893 2595 4091 1645
Size: 248 702 1496 1768
Fragments arranged by size:
1768 1496 702 248
BsaWI w'CCGG_w
Cuts at: 615 1403 1924 2602 2876 3023 3434 615
Size: 788 521 678 274 147 411 1395
Fragments arranged by size:
1395 788 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 423 809 1019 423
Size: 386 210 3618
Fragments arranged by size:
3618 386 210
BseYI C'CCAG_C
Cuts at: 500 1147 1152 1639 2397 2974 3470 500
Size: 647 5 487 758 577 496 1244
Fragments arranged by size:
1244 758 647 577 496 487 5
BsiEI CG_ry'CG
Cuts at: 104 1756 1802 3010 104
Size: 1652 46 1208 1308
Fragments arranged by size:
1652 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 190 1228 2006 2196 2988 190
Size: 1038 778 190 792 1416
Fragments arranged by size:
1416 1038 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 190 779 836 845 1052 1228 1839 1932 2006
Size: 589 57 9 207 176 611 93 74
Cuts at: 2006 2196 2258 2988 190
Size: 190 62 730 1416
Fragments arranged by size:
1416 730 611 589 207 190 176 93
74 62 57 9
BspEI T'CCGG_A
Cuts at: 3434 3434
Size: 4214
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 238 717 796 1264 1478 1780 2161 238
Size: 479 79 468 214 302 381 2291
Fragments arranged by size:
2291 479 468 381 302 214 79
BsrBI CCG'CTC
Cuts at: 50 221 2506 2560 4044 4209 50
Size: 171 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2126 2126
Size: 4214
BsrFI r'CCGG_y
Cuts at: 2212 2393 2602 2212
Size: 181 209 3824
Fragments arranged by size:
3824 209 181
BssSI C'ACGA_G
Cuts at: 936 1000 1031 2485 2843 936
Size: 64 31 1454 358 2307
Fragments arranged by size:
2307 1454 358 64 31
BstAPI GCAn_nnn'nTGC
Cuts at: 1591 1591
Size: 4214
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstXI CCAn_nnnn'nTGG
Cuts at: 889 1129 889
Size: 240 3974
BstYI r'GATC_y
Cuts at: 1 796 1067 1239 1706 1729 2064 2310 3322
Size: 795 271 172 467 23 335 246 1012
Cuts at: 3322 3408 3420 3766 1
Size: 86 12 346 449
Fragments arranged by size:
1012 795 467 449 346 335 271 246
172 86 23 12
BtgI C'CryG_G
Cuts at: 107 249 516 107
Size: 142 267 3805
Fragments arranged by size:
3805 267 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2164 2345 2508 2164
Size: 181 163 3870
Fragments arranged by size:
3870 181 163
BtsI GCAGTG_nn'
Cuts at: 138 1457 138
Size: 1319 2895
DraI TTT'AAA
Cuts at: 1213 1213
Size: 4214
DrdI GACnn_nn'nnGTC
Cuts at: 1920 2778 1920
Size: 858 3356
EaeI y'GGCC_r
Cuts at: 1179 1799 1973 2364 2391 3736 1179
Size: 620 174 391 27 1345 1657
Fragments arranged by size:
1657 1345 620 391 174 27
EagI C'GGCC_G
Cuts at: 1799 1799
Size: 4214
EarI CTCTTCn'nnn_
Cuts at: 298 911 1107 2237 2447 298
Size: 613 196 1130 210 2065
Fragments arranged by size:
2065 1130 613 210 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2422 2732 2878 4024 2422
Size: 310 146 1146 2612
Fragments arranged by size:
2612 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1226 1226
Size: 4214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 429 641 742 848 865 892 950 1013
Size: 77 212 101 106 17 27 58 63
Cuts at: 1013 1127 2039 2471 3218 3486 352
Size: 114 912 432 747 268 1080
Fragments arranged by size:
1080 912 747 432 268 212 114 106
101 77 63 58 27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 295 369 1106 295
Size: 74 737 3403
Fragments arranged by size:
3403 737 74
EcoO109I rG'GnC_Cy
Cuts at: 471 636 776 841 842 1353 471
Size: 165 140 65 1 511 3332
Fragments arranged by size:
3332 511 165 140 65 1
EcoRI G'AATT_C
Cuts at: 1218 1218
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 1995 1995
Size: 4214
HaeII r_GCGC'y
Cuts at: 285 339 1648 1896 2918 3897 285
Size: 54 1309 248 1022 979 602
Fragments arranged by size:
1309 1022 979 602 248 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 804 836 942 974 2056 2086 2088 2118 804
Size: 32 106 32 1082 30 2 30 2900
Fragments arranged by size:
2900 1082 106 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1253 1253
Size: 4214
KasI G'GCGC_C
Cuts at: 1644 1892 1644
Size: 248 3966
KpnI G_GTAC'C
Cuts at: 1234 1234
Size: 4214
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 250 2885 3069 250
Size: 2635 184 1395
Fragments arranged by size:
2635 1395 184
MscI TGG'CCA
Cuts at: 1975 1975
Size: 4214
MslI CAynn'nnrTG
Cuts at: 317 1085 2203 2330 317
Size: 768 1118 127 2201
Fragments arranged by size:
2201 1118 768 127
MspA1I CmG'CkG
Cuts at: 239 762 975 991 1325 1639 1999 3012 3257
Size: 523 213 16 334 314 360 1013 245
Cuts at: 3257 239
Size: 1196
Fragments arranged by size:
1196 1013 523 360 334 314 245 213
16
NaeI GCC'GGC
Cuts at: 2395 2395
Size: 4214
NarI GG'CG_CC
Cuts at: 1645 1893 1645
Size: 248 3966
NcoI C'CATG_G
Cuts at: 107 249 516 107
Size: 142 267 3805
Fragments arranged by size:
3805 267 142
NgoMIV G'CCGG_C
Cuts at: 2393 2393
Size: 4214
NspI r_CATG'y
Cuts at: 126 1267 1767 2674 126
Size: 1141 500 907 1666
Fragments arranged by size:
1666 1141 907 500
PasI CC'CwG_GG
Cuts at: 161 161
Size: 4214
PciI A'CATG_T
Cuts at: 2670 2670
Size: 4214
PfoI T'CCnGG_A
Cuts at: 329 607 329
Size: 278 3936
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 4182
PpuMI rG'GwC_Cy
Cuts at: 471 636 471
Size: 165 4049
PspOMI G'GGCC_C
Cuts at: 775 841 775
Size: 66 4148
PstI C_TGCA'G
Cuts at: 980 1261 1946 980
Size: 281 685 3248
Fragments arranged by size:
3248 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 762 975 991 1639 1999 762
Size: 213 16 648 360 2977
Fragments arranged by size:
2977 648 360 213 16
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 1228 1228
Size: 4214
SalI G'TCGA_C
Cuts at: 1251 1251
Size: 4214
SanDI GG'GwC_CC
Cuts at: 636 636
Size: 4214
SapI GCTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 809 3731 809
Size: 2922 1292
SfcI C'TryA_G
Cuts at: 33 913 976 1257 1942 2935 3126 33
Size: 880 63 281 685 993 191 1121
Fragments arranged by size:
1121 993 880 685 281 191 63
SfoI GGC'GCC
Cuts at: 1646 1894 1646
Size: 248 3966
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 549 1236 549
Size: 687 3527
SmlI C'TyrA_G
Cuts at: 268 1369 2284 2776 3038 268
Size: 1101 915 492 262 1444
Fragments arranged by size:
1444 1101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4214
SphI G_CATG'C
Cuts at: 1267 1767 1267
Size: 500 3714
StuI AGG'CCT
Cuts at: 925 925
Size: 4214
StyI C'CwwG_G
Cuts at: 107 249 516 631 837 1348 1702 107
Size: 142 267 115 206 511 354 2619
Fragments arranged by size:
2619 511 354 267 206 142 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 818 1797 1930 2389 2598 818
Size: 979 133 459 209 2434
Fragments arranged by size:
2434 979 459 209 133
TatI w'GTAC_w
Cuts at: 807 3729 807
Size: 2922 1292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3731 3763 3731
Size: 32 4182
Tth111I GACn'n_nGTC
Cuts at: 2011 2011
Size: 4214
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1709 1709
Size: 4214
XmaI C'CCGG_G
Cuts at: 547 1234 547
Size: 687 3527
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI