Restriction map for
pF1KB7621
(Circular) (Six-base) MAPSORT of: pF1KB7621.seq Check: 2080 from: 1 to: 4052
pF1KB7621 4052 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 702 985 1357 2924 702
Size: 283 372 1567 1830
Fragments arranged by size:
1830 1567 372 283
ApaI G_GGCC'C
Cuts at: 548 548
Size: 4052
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 288 1072 288
Size: 784 3268
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 186 316 358 1068 1482 1730 1765 186
Size: 130 42 710 414 248 35 2473
Fragments arranged by size:
2473 710 414 248 130 42 35
BanII G_rGCy'C
Cuts at: 548 782 950 1066 2096 548
Size: 234 168 116 1030 2504
Fragments arranged by size:
2504 1030 234 168 116
BbeI G_GCGC'C
Cuts at: 190 320 362 1486 1734 190
Size: 130 42 1124 248 2508
Fragments arranged by size:
2508 1124 248 130 42
BbsI GAAGACnn'nnnn_
Cuts at: 956 956
Size: 4052
BbvCI CC'TCA_GC
Cuts at: 917 917
Size: 4052
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglI GCCn_nnn'nGGC
Cuts at: 170 300 170
Size: 130 3922
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 548 1677 1770 2826 548
Size: 1129 93 1056 1774
Fragments arranged by size:
1774 1129 1056 93
BmgBI CAC'GTC
Cuts at: 138 138
Size: 4052
BmrI ACTGGGnnnn_n'
Cuts at: 1025 1264 1345 1680 3824 1025
Size: 239 81 335 2144 1253
Fragments arranged by size:
2144 1253 335 239 81
BmtI G_CTAG'C
Cuts at: 766 766
Size: 4052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 730 724
Size: 6 4046
Bpu10I CC'TnA_GC
Cuts at: 917 917
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 972 1228 2107 2599 2897 972
Size: 256 879 492 298 2127
Fragments arranged by size:
2127 879 492 298 256
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 114 240 1405 114
Size: 126 1165 2761
Fragments arranged by size:
2761 1165 126
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 187 317 359 1483 1731 2433 3929 187
Size: 130 42 1124 248 702 1496 310
Fragments arranged by size:
1496 1124 702 310 248 130 42
BsaWI w'CCGG_w
Cuts at: 746 957 1241 1762 2440 2714 2861 3272 746
Size: 211 284 521 678 274 147 411 1526
Fragments arranged by size:
1526 678 521 411 284 274 211 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 388 418 703 733 139
Size: 30 219 30 285 30 3458
Fragments arranged by size:
3458 285 219 30 30 30
BseYI C'CCAG_C
Cuts at: 774 911 1477 2235 2812 3308 774
Size: 137 566 758 577 496 1518
Fragments arranged by size:
1518 758 577 566 496 137
BsiEI CG_ry'CG
Cuts at: 104 248 368 413 488 1594 1640 2848 104
Size: 144 120 45 75 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 144 120 75 46 45
BsiHKAI G_wGCw'C
Cuts at: 1066 1844 2034 2826 1066
Size: 778 190 792 2292
Fragments arranged by size:
2292 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 548 782 950 1066 1677 1770 1844 2034 2096
Size: 234 168 116 611 93 74 190 62
Cuts at: 2096 2826 548
Size: 730 1774
Fragments arranged by size:
1774 730 611 234 190 168 116 93
74 62
BspEI T'CCGG_A
Cuts at: 746 3272 746
Size: 2526 1526
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 783 1102 1316 1618 1999 783
Size: 319 214 302 381 2836
Fragments arranged by size:
2836 381 319 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 303 621 2050 2231 2440 303
Size: 318 1429 181 209 1915
Fragments arranged by size:
1915 1429 318 209 181
BsrGI T'GTAC_A
Cuts at: 683 683
Size: 4052
BssHII G'CGCG_C
Cuts at: 278 278
Size: 4052
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstEII G'GTnAC_C
Cuts at: 232 232
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 381 381
Size: 4052
BstYI r'GATC_y
Cuts at: 1 1077 1544 1567 1902 2148 3160 3246 3258
Size: 1076 467 23 335 246 1012 86 12
Cuts at: 3258 3604 1
Size: 346 449
Fragments arranged by size:
1076 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 362 380 419 362
Size: 18 39 3995
Fragments arranged by size:
3995 39 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 245 301 365 410 485 585 1637 1811 2202
Size: 56 64 45 75 100 1052 174 391
Cuts at: 2202 2229 3574 245
Size: 27 1345 723
Fragments arranged by size:
1345 1052 723 391 174 100 75 64
56 45 27
EagI C'GGCC_G
Cuts at: 245 365 410 485 1637 245
Size: 120 45 75 1152 2660
Fragments arranged by size:
2660 1152 120 75 45
EarI CTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 609 881 2260 2570 2716 3862 609
Size: 272 1379 310 146 1146 799
Fragments arranged by size:
1379 1146 799 310 272 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 730 1877 2309 3056 3324 724
Size: 6 1147 432 747 268 1452
Fragments arranged by size:
1452 1147 747 432 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 130 920 130
Size: 790 3262
EcoO109I rG'GnC_Cy
Cuts at: 925 1191 925
Size: 266 3786
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 190 320 362 1486 1734 2756 3735 190
Size: 130 42 1124 248 1022 979 507
Fragments arranged by size:
1124 1022 979 507 248 130 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 559 591 1894 1924 1926 1956 559
Size: 32 1303 30 2 30 2655
Fragments arranged by size:
2655 1303 32 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 186 316 358 1482 1730 186
Size: 130 42 1124 248 2508
Fragments arranged by size:
2508 1124 248 130 42
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 1813 1813
Size: 4052
MslI CAynn'nnrTG
Cuts at: 112 2041 2168 112
Size: 1929 127 1996
Fragments arranged by size:
1996 1929 127
MspA1I CmG'CkG
Cuts at: 253 259 364 559 573 591 699 1163 1477
Size: 6 105 195 14 18 108 464 314
Cuts at: 1477 1837 2850 3095 253
Size: 360 1013 245 1210
Fragments arranged by size:
1210 1013 464 360 314 245 195 108
105 18 14 6
NaeI GCC'GGC
Cuts at: 305 623 2233 305
Size: 318 1610 2124
Fragments arranged by size:
2124 1610 318
NarI GG'CG_CC
Cuts at: 187 317 359 1483 1731 187
Size: 130 42 1124 248 2508
Fragments arranged by size:
2508 1124 248 130 42
NcoI C'CATG_G
Cuts at: 419 419
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 303 621 2231 303
Size: 318 1610 2124
Fragments arranged by size:
2124 1610 318
NheI G'CTAG_C
Cuts at: 762 762
Size: 4052
NotI GC'GGCC_GC
Cuts at: 245 365 245
Size: 120 3932
NspI r_CATG'y
Cuts at: 147 1105 1605 2512 147
Size: 958 500 907 1687
Fragments arranged by size:
1687 958 907 500
PasI CC'CwG_GG
Cuts at: 997 997
Size: 4052
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PflMI CCAn_nnn'nTGG
Cuts at: 702 702
Size: 4052
PfoI T'CCnGG_A
Cuts at: 125 326 125
Size: 201 3851
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PmlI CAC'GTG
Cuts at: 240 240
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PpuMI rG'GwC_Cy
Cuts at: 925 925
Size: 4052
PspOMI G'GGCC_C
Cuts at: 544 544
Size: 4052
PstI C_TGCA'G
Cuts at: 258 1099 1784 258
Size: 841 685 2526
Fragments arranged by size:
2526 841 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 253 573 1477 1837 253
Size: 320 904 360 2468
Fragments arranged by size:
2468 904 360 320
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SacII CC_GC'GG
Cuts at: 365 365
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 254 426 726 1095 1780 2773 2964 33
Size: 221 172 300 369 685 993 191 1121
Fragments arranged by size:
1121 993 685 369 300 221 191 172
SfiI GGCCn_nnn'nGGCC
Cuts at: 300 300
Size: 4052
SfoI GGC'GCC
Cuts at: 188 318 360 1484 1732 188
Size: 130 42 1124 248 2508
Fragments arranged by size:
2508 1124 248 130 42
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 290 1074 290
Size: 784 3268
SmlI C'TyrA_G
Cuts at: 951 1207 2122 2614 2876 951
Size: 256 915 492 262 2127
Fragments arranged by size:
2127 915 492 262 256
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 419 1186 1540 419
Size: 767 354 2931
Fragments arranged by size:
2931 767 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1057 1635 1768 2227 2436 1057
Size: 578 133 459 209 2673
Fragments arranged by size:
2673 578 459 209 133
TatI w'GTAC_w
Cuts at: 683 3567 683
Size: 2884 1168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 288 1072 288
Size: 784 3268
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BseRI BsgI BsiWI
BsmI BsmBI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NruI NsiI
PacI PshAI PsiI PspXI PsrI SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI