Restriction map for
pF1KB7624
(Circular) (Six-base) MAPSORT of: pF1KB7624.seq Check: 1367 from: 1 to: 4217
pF1KB7624 4217 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1255 1255
Size: 4217
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 593 2042 2474 3221 3489 430
Size: 163 1449 432 747 268 1158
Fragments arranged by size:
1449 1158 747 432 268 163
AflIII A'CryG_T
Cuts at: 263 681 1209 1569 2673 3650 263
Size: 418 528 360 1104 977 830
Fragments arranged by size:
1104 977 830 528 418 360
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 485 485
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 1522 3089 1522
Size: 1567 2650
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 249 433 560 586 852 1221 3570 3826 249
Size: 184 127 26 266 369 2349 256 640
Fragments arranged by size:
2349 640 369 266 256 184 127 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 1237 1237
Size: 4217
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 1233 1647 1895 1930 1233
Size: 414 248 35 3520
Fragments arranged by size:
3520 414 248 35
BanII G_rGCy'C
Cuts at: 203 1231 2261 203
Size: 1028 1030 2159
Fragments arranged by size:
2159 1030 1028
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BbvCI CC'TCA_GC
Cuts at: 721 721
Size: 4217
BciVI GTATCCnnnnn_n'
Cuts at: 2115 2876 4217 2115
Size: 761 1341 2115
Fragments arranged by size:
2115 1341 761
BclI T'GATC_A
Cuts at: 660 1737 660
Size: 1077 3140
BglII A'GATC_T
Cuts at: 1732 3769 1732
Size: 2037 2180
BlpI GC'TnA_GC
Cuts at: 151 1329 151
Size: 1178 3039
Bme1580I G_kGCm'C
Cuts at: 1842 1935 2991 1842
Size: 93 1056 3068
Fragments arranged by size:
3068 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1429 1510 1845 3989 1429
Size: 81 335 2144 1657
Fragments arranged by size:
2144 1657 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 225
Size: 4217
Bpu10I CC'TnA_GC
Cuts at: 721 721
Size: 4217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2272 2764 3062 1393
Size: 879 492 298 2548
Fragments arranged by size:
2548 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3921 3921
Size: 4217
BsaAI yAC'GTr
Cuts at: 1570 1570
Size: 4217
BsaBI GATnn'nnATC
Cuts at: 6 665 1731 3768 6
Size: 659 1066 2037 455
Fragments arranged by size:
2037 1066 659 455
BsaHI Gr'CG_yC
Cuts at: 1648 1896 2598 4094 1648
Size: 248 702 1496 1771
Fragments arranged by size:
1771 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1406 1927 2605 2879 3026 3437 1406
Size: 521 678 274 147 411 2186
Fragments arranged by size:
2186 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 107 1215 107
Size: 1108 3109
BseYI C'CCAG_C
Cuts at: 1642 2400 2977 3473 1642
Size: 758 577 496 2386
Fragments arranged by size:
2386 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1111
Size: 4217
BsiEI CG_ry'CG
Cuts at: 104 1759 1805 3013 104
Size: 1655 46 1208 1308
Fragments arranged by size:
1655 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 1231 2009 2199 2991 203
Size: 1028 778 190 792 1429
Fragments arranged by size:
1429 1028 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 160 160
Size: 4217
Bsp1286I G_dGCh'C
Cuts at: 203 1231 1842 1935 2009 2199 2261 2991 203
Size: 1028 611 93 74 190 62 730 1429
Fragments arranged by size:
1429 1028 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3437 3437
Size: 4217
BspHI T'CATG_A
Cuts at: 657 3393 657
Size: 2736 1481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1267 1481 1783 2164 1267
Size: 214 302 381 3320
Fragments arranged by size:
3320 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2509 2563 4047 4212 50
Size: 2459 54 1484 165 55
Fragments arranged by size:
2459 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 530 1092 2129 530
Size: 562 1037 2618
Fragments arranged by size:
2618 1037 562
BsrFI r'CCGG_y
Cuts at: 2215 2396 2605 2215
Size: 181 209 3827
Fragments arranged by size:
3827 209 181
BssSI C'ACGA_G
Cuts at: 2488 2846 2488
Size: 358 3859
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstYI r'GATC_y
Cuts at: 1 1242 1709 1732 2067 2313 3325 3411 3423
Size: 1241 467 23 335 246 1012 86 12
Cuts at: 3423 3769 1
Size: 346 449
Fragments arranged by size:
1241 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 787 1184 1460 787
Size: 397 276 3544
Fragments arranged by size:
3544 397 276
DraI TTT'AAA
Cuts at: 431 1216 431
Size: 785 3432
DrdI GACnn_nn'nnGTC
Cuts at: 1923 2781 1923
Size: 858 3359
EaeI y'GGCC_r
Cuts at: 1802 1976 2367 2394 3739 1802
Size: 174 391 27 1345 2280
Fragments arranged by size:
2280 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1802 1802
Size: 4217
EarI CTCTTCn'nnn_
Cuts at: 978 1148 1192 2240 2450 978
Size: 170 44 1048 210 2745
Fragments arranged by size:
2745 1048 210 170 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 2735 2881 4027 2425
Size: 310 146 1146 2615
Fragments arranged by size:
2615 1146 310 146
EcoICRI GAG'CTC
Cuts at: 201 1229 201
Size: 1028 3189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 430 593 2042 2474 3221 3489 225
Size: 205 163 1449 432 747 268 953
Fragments arranged by size:
1449 953 747 432 268 205 163
EcoO109I rG'GnC_Cy
Cuts at: 594 1356 594
Size: 762 3455
EcoRI G'AATT_C
Cuts at: 1221 1221
Size: 4217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 4217
FspI TGC'GCA
Cuts at: 1998 1998
Size: 4217
HaeII r_GCGC'y
Cuts at: 148 1651 1899 2921 3900 148
Size: 1503 248 1022 979 465
Fragments arranged by size:
1503 1022 979 465 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 976 1008 2059 2089 2091 2121 976
Size: 32 1051 30 2 30 3072
Fragments arranged by size:
3072 1051 32 30 30 2
HincII GTy'rAC
Cuts at: 382 1256 382
Size: 874 3343
HindIII A'AGCT_T
Cuts at: 278 278
Size: 4217
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2888 3072 2888
Size: 184 4033
MscI TGG'CCA
Cuts at: 1978 1978
Size: 4217
MslI CAynn'nnrTG
Cuts at: 467 2206 2333 467
Size: 1739 127 2351
Fragments arranged by size:
2351 1739 127
MspA1I CmG'CkG
Cuts at: 1328 1642 2002 3015 3260 1328
Size: 314 360 1013 245 2285
Fragments arranged by size:
2285 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2398 2398
Size: 4217
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NcoI C'CATG_G
Cuts at: 107 107
Size: 4217
NgoMIV G'CCGG_C
Cuts at: 2396 2396
Size: 4217
NspI r_CATG'y
Cuts at: 267 685 1270 1770 2677 267
Size: 418 585 500 907 1807
Fragments arranged by size:
1807 907 585 500 418
PciI A'CATG_T
Cuts at: 263 681 2673 263
Size: 418 1992 1807
Fragments arranged by size:
1992 1807 418
PfoI T'CCnGG_A
Cuts at: 161 161
Size: 4217
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PpuMI rG'GwC_Cy
Cuts at: 594 594
Size: 4217
PstI C_TGCA'G
Cuts at: 131 1264 1949 131
Size: 1133 685 2399
Fragments arranged by size:
2399 1133 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 1642 2002 1642
Size: 360 3857
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 203 1231 203
Size: 1028 3189
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 3734 3734
Size: 4217
SfcI C'TryA_G
Cuts at: 33 127 1260 1945 2938 3129 33
Size: 94 1133 685 993 191 1121
Fragments arranged by size:
1133 1121 993 685 191 94
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 1239 1239
Size: 4217
SmlI C'TyrA_G
Cuts at: 1372 2287 2779 3041 1372
Size: 915 492 262 2548
Fragments arranged by size:
2548 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
SspI AAT'ATT
Cuts at: 753 753
Size: 4217
StuI AGG'CCT
Cuts at: 913 913
Size: 4217
StyI C'CwwG_G
Cuts at: 107 1351 1705 107
Size: 1244 354 2619
Fragments arranged by size:
2619 1244 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 1800 1933 2392 2601 148
Size: 1652 133 459 209 1764
Fragments arranged by size:
1764 1652 459 209 133
TatI w'GTAC_w
Cuts at: 3732 3732
Size: 4217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4185
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 331 1712 331
Size: 1381 2836
XmaI C'CCGG_G
Cuts at: 1237 1237
Size: 4217
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI