Restriction map for
pF1KB7625
(Circular) (Six-base) MAPSORT of: pF1KB7625.seq Check: 3162 from: 1 to: 4061
pF1KB7625 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1886 2318 3065 3333 508
Size: 1378 432 747 268 1236
Fragments arranged by size:
1378 1236 747 432 268
AflII C'TTAA_G
Cuts at: 303 303
Size: 4061
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 581 2449 581
Size: 1868 2193
AleI CACnn'nnGTG
Cuts at: 1001 1001
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 1366 2933 1366
Size: 1567 2494
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 482 631 664 1051 1065 3414 3670 482
Size: 149 33 387 14 2349 256 873
Fragments arranged by size:
2349 873 387 256 149 33 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BamHI G'GATC_C
Cuts at: 147 367 1086 147
Size: 220 719 3122
Fragments arranged by size:
3122 719 220
BanI G'GyrC_C
Cuts at: 960 1077 1491 1739 1774 960
Size: 117 414 248 35 3247
Fragments arranged by size:
3247 414 248 117 35
BanII G_rGCy'C
Cuts at: 1075 2105 1075
Size: 1030 3031
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 812 812
Size: 4061
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 963 1686 1779 2835 963
Size: 723 93 1056 2189
Fragments arranged by size:
2189 1056 723 93
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 150 581 1250 1771 2449 2723 2870 3281 150
Size: 431 669 521 678 274 147 411 930
Fragments arranged by size:
930 678 669 521 431 411 274 147
BseYI C'CCAG_C
Cuts at: 248 1486 2244 2821 3317 248
Size: 1238 758 577 496 992
Fragments arranged by size:
1238 992 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 4061
BsiEI CG_ry'CG
Cuts at: 104 155 1603 1649 2857 104
Size: 51 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 51 46
BsiHKAI G_wGCw'C
Cuts at: 1075 1853 2043 2835 1075
Size: 778 190 792 2301
Fragments arranged by size:
2301 792 778 190
BsmI GAATG_Cn'
Cuts at: 354 354
Size: 4061
Bsp1286I G_dGCh'C
Cuts at: 963 1075 1686 1779 1853 2043 2105 2835 963
Size: 112 611 93 74 190 62 730 2189
Fragments arranged by size:
2189 730 611 190 112 93 74 62
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 1111 1325 1627 2008 578
Size: 533 214 302 381 2631
Fragments arranged by size:
2631 533 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 581 2059 2240 2449 581
Size: 1478 181 209 2193
Fragments arranged by size:
2193 1478 209 181
BsrGI T'GTAC_A
Cuts at: 914 914
Size: 4061
BssSI C'ACGA_G
Cuts at: 692 2332 2690 692
Size: 1640 358 2063
Fragments arranged by size:
2063 1640 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 951 1003 951
Size: 52 4009
BstYI r'GATC_y
Cuts at: 1 147 297 367 1086 1553 1576 1911 2157
Size: 146 150 70 719 467 23 335 246
Cuts at: 2157 3169 3255 3267 3613 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 719 467 449 346 335 246 150
146 86 70 23 12
BtgI C'CryG_G
Cuts at: 107 800 944 107
Size: 693 144 3224
Fragments arranged by size:
3224 693 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 897 1304 897
Size: 407 3654
DraI TTT'AAA
Cuts at: 663 1060 663
Size: 397 3664
DraIII CAC_nnn'GTG
Cuts at: 967 967
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 1646 1820 2211 2238 3583 1646
Size: 174 391 27 1345 2124
Fragments arranged by size:
2124 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2269 2579 2725 3871 127
Size: 2142 310 146 1146 317
Fragments arranged by size:
2142 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1886 2318 3065 3333 508
Size: 1378 432 747 268 1236
Fragments arranged by size:
1378 1236 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1200 1200
Size: 4061
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
EcoRV GAT'ATC
Cuts at: 441 441
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 642 1903 1933 1935 1965 610
Size: 32 1261 30 2 30 2706
Fragments arranged by size:
2706 1261 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
HindIII A'AGCT_T
Cuts at: 508 508
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 275 2732 2916 275
Size: 2457 184 1420
Fragments arranged by size:
2457 1420 184
MscI TGG'CCA
Cuts at: 1822 1822
Size: 4061
MslI CAynn'nnrTG
Cuts at: 1001 2050 2177 1001
Size: 1049 127 2885
Fragments arranged by size:
2885 1049 127
MspA1I CmG'CkG
Cuts at: 255 1172 1486 1846 2859 3104 255
Size: 917 314 360 1013 245 1212
Fragments arranged by size:
1212 1013 917 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 107 800 107
Size: 693 3368
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 2521 1114
Size: 500 907 2654
Fragments arranged by size:
2654 907 500
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PstI C_TGCA'G
Cuts at: 575 1108 1793 575
Size: 533 685 2843
Fragments arranged by size:
2843 685 533
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 1486 1846 1486
Size: 360 3701
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 530 571 670 1104 1789 2782 2973 33
Size: 497 41 99 434 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 434 191 99 41
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 303 1216 2131 2623 2885 303
Size: 913 915 492 262 1479
Fragments arranged by size:
1479 915 913 492 262
SpeI A'CTAG_T
Cuts at: 67 387 67
Size: 320 3741
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 107 677 800 1195 1549 107
Size: 570 123 395 354 2619
Fragments arranged by size:
2619 570 395 354 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1644 1777 2236 2445 1644
Size: 133 459 209 3260
Fragments arranged by size:
3260 459 209 133
TatI w'GTAC_w
Cuts at: 914 3576 914
Size: 2662 1399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4029
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 196 1556 196
Size: 1360 2701
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
XmnI GAAnn'nnTTC
Cuts at: 729 729
Size: 4061
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI