Restriction map for
pF1KB7627
(Circular) (Six-base) MAPSORT of: pF1KB7627.seq Check: 2756 from: 1 to: 4004
pF1KB7627 4004 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 166 166
Size: 4004
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1829 2261 3008 3276 1829
Size: 432 747 268 2557
Fragments arranged by size:
2557 747 432 268
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AhdI GACnn_n'nnGTC
Cuts at: 151 151
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 1309 2876 1309
Size: 1567 2437
ApaLI G'TGCA_C
Cuts at: 426 2774 426
Size: 2348 1656
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 760 1024 760
Size: 264 3740
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 1020 1434 1682 1717 1020
Size: 414 248 35 3307
Fragments arranged by size:
3307 414 248 35
BanII G_rGCy'C
Cuts at: 578 971 1018 2048 578
Size: 393 47 1030 2534
Fragments arranged by size:
2534 1030 393 47
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 430 506 980 1629 1722 2778 430
Size: 76 474 649 93 1056 1656
Fragments arranged by size:
1656 1056 649 474 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 141 855 1216 1297 1632 3776 141
Size: 714 361 81 335 2144 369
Fragments arranged by size:
2144 714 369 361 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 855 2371 855
Size: 1516 2488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 974 985 935
Size: 39 11 3954
Fragments arranged by size:
3954 39 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 691 916 3708 691
Size: 225 2792 987
Fragments arranged by size:
2792 987 225
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 233
Size: 4004
BseYI C'CCAG_C
Cuts at: 263 688 740 991 1429 2187 2764 3260 263
Size: 425 52 251 438 758 577 496 1007
Fragments arranged by size:
1007 758 577 496 438 425 251 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 315 807 213
Size: 102 492 3410
Fragments arranged by size:
3410 492 102
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 430 971 1018 1796 1986 2778 430
Size: 541 47 778 190 792 1656
Fragments arranged by size:
1656 792 778 541 190 47
BsmI GAATG_Cn'
Cuts at: 871 871
Size: 4004
Bsp1286I G_dGCh'C
Cuts at: 430 506 578 971 980 1018 1629 1722 1796
Size: 76 72 393 9 38 611 93 74
Cuts at: 1796 1986 2048 2778 430
Size: 190 62 730 1656
Fragments arranged by size:
1656 730 611 393 190 93 76 74
72 62 38 9
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 166 239 890 1054 1268 1570 1951 166
Size: 73 651 164 214 302 381 2219
Fragments arranged by size:
2219 651 381 302 214 164 73
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 612 1916 612
Size: 1304 2700
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 236 425 1381 236
Size: 189 956 2859
Fragments arranged by size:
2859 956 189
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 169 169
Size: 4004
BstYI r'GATC_y
Cuts at: 1 1029 1496 1519 1854 2100 3112 3198 3210
Size: 1028 467 23 335 246 1012 86 12
Cuts at: 3210 3556 1
Size: 346 449
Fragments arranged by size:
1028 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 509 509
Size: 4004
BtgI C'CryG_G
Cuts at: 107 812 107
Size: 705 3299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 1247
Size: 4004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 180 215 507 542 180
Size: 35 292 35 3642
Fragments arranged by size:
3642 292 35 35
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 1589 1763 2154 2181 3526 1589
Size: 174 391 27 1345 2067
Fragments arranged by size:
2067 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 583 679 2027 2237 583
Size: 96 1348 210 2350
Fragments arranged by size:
2350 1348 210 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2522 2668 3814 2212
Size: 310 146 1146 2402
Fragments arranged by size:
2402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 969 1016 969
Size: 47 3957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 974 985 1829 2261 3008 3276 935
Size: 39 11 844 432 747 268 1663
Fragments arranged by size:
1663 844 747 432 268 39 11
EcoO109I rG'GnC_Cy
Cuts at: 1143 1143
Size: 4004
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 618 2023 618
Size: 1405 2599
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 760 792 1846 1876 1878 1908 137
Size: 32 591 32 1054 30 2 30 2233
Fragments arranged by size:
2233 1054 591 32 32 30 30 2
HincII GTy'rAC
Cuts at: 327 1043 327
Size: 716 3288
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 1765 1765
Size: 4004
MslI CAynn'nnrTG
Cuts at: 1993 2120 1993
Size: 127 3877
MspA1I CmG'CkG
Cuts at: 263 1115 1429 1789 2802 3047 263
Size: 852 314 360 1013 245 1220
Fragments arranged by size:
1220 1013 852 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NcoI C'CATG_G
Cuts at: 107 812 107
Size: 705 3299
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 1057 1557 2464 1057
Size: 500 907 2597
Fragments arranged by size:
2597 907 500
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PfoI T'CCnGG_A
Cuts at: 174 174
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PspXI vC'TCGA_Gb
Cuts at: 760 760
Size: 4004
PstI C_TGCA'G
Cuts at: 407 638 775 1051 1736 407
Size: 231 137 276 685 2675
Fragments arranged by size:
2675 685 276 231 137
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 263 1429 1789 263
Size: 1166 360 2478
Fragments arranged by size:
2478 1166 360
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 971 1018 971
Size: 47 3957
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 583 2027 2237 583
Size: 1444 210 2350
Fragments arranged by size:
2350 1444 210
SbfI CC_TGCA'GG
Cuts at: 407 1051 407
Size: 644 3360
ScaI AGT'ACT
Cuts at: 130 3521 130
Size: 3391 613
SfcI C'TryA_G
Cuts at: 33 403 634 771 1047 1732 2725 2916 33
Size: 370 231 137 276 685 993 191 1121
Fragments arranged by size:
1121 993 685 370 276 231 191 137
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 760 1159 2074 2566 2828 760
Size: 399 915 492 262 1936
Fragments arranged by size:
1936 915 492 399 262
SpeI A'CTAG_T
Cuts at: 67 781 67
Size: 714 3290
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StuI AGG'CCT
Cuts at: 401 401
Size: 4004
StyI C'CwwG_G
Cuts at: 107 191 812 926 1138 1492 107
Size: 84 621 114 212 354 2619
Fragments arranged by size:
2619 621 354 212 114 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 510 702 1587 1720 2179 2388 510
Size: 192 885 133 459 209 2126
Fragments arranged by size:
2126 885 459 209 192 133
TatI w'GTAC_w
Cuts at: 128 3519 128
Size: 3391 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 400 432 3521 3553 400
Size: 32 3089 32 851
Fragments arranged by size:
3089 851 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1499 1499
Size: 4004
XhoI C'TCGA_G
Cuts at: 760 760
Size: 4004
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI